Labshake search
Citations for New England Biolabs :
4151 - 4200 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Libraries were size selected for cDNA target fragments of 300 bp on 2% Low Range Ultra Agarose followed by PCR amplification using Phusion DNA polymerase (NEB) for 15 cycles ...
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bioRxiv - Immunology 2022Quote: ... The cDNA libraries were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina 96 reactions (New England Biolabs). Libraries were quantified using the KAPA Library Quantification Kit (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... Library preparation was performed using NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, Ipswich, MA, USA) and SMRTbell Express Template Prep Kit 2.0 (PacBio) ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were selected for cDNA target fragments of 300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles ...
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bioRxiv - Immunology 2024Quote: ... Oxford Genomics Centre prepared bulk RNA libraries using an NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced the samples at a depth of 25 million reads per sample on a NovaSeq6000 (Illumina) ...
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared by the West Virginia University Genomics Core using the NEBNext Low Input/Single Cell RNA-Seq kit (New England Biolabs). Libraries were sequenced to 30 million reads on a Nextseq 2000 (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were then size selected for cDNA target fragments of 200–300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were then size-selected for cDNA target fragments of 200–300 bp on 2% Low Range Ultra Agarose followed by PCR amplification using Phusion DNA polymerase (NEB) for 15 PCR cycles ...
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bioRxiv - Biochemistry 2023Quote: ... Libraries were size selected for cDNA target fragments of 300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles ...
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bioRxiv - Immunology 2023Quote: ... Live CD19+ B cells and CD4+PD1+CXCR5- Tph cells were purified by high-speed sorting (FACSAria II) and were directly processed using NEBNext Single Cell/Low Input RNA Library Prep Kit (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 100 cells were isolated directly into low protein binding eppendorfs containing 2 ul NEBNext Single Cell Lysis Buffer (NEB, E5530S). Samples were kept on dry ice until transfer to −80 C for overnight storage.
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bioRxiv - Immunology 2023Quote: ... The RNA sequencing libraries were generated using NEB Next Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) according to manufacturer’s protocol and sequenced on a NovaSeq 6000 sequencer (Illumina ...
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bioRxiv - Immunology 2023Quote: RNA-seq libraries were generated following the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB #E6420S) protocol for cells ...
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bioRxiv - Physiology 2023Quote: ... between 400 and 3000 pg of total RNA were used to generate barcoded RNA-seq libraries using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Isoseq library preparation was performed on 300 ng of RNA (RIN ≥ 9.3) of each cell type (isolated as described above) using NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification (NEB), Iso-Seq Express Oligo Kit (PacBio ...
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bioRxiv - Immunology 2023Quote: RNA of the carriers and non-carriers were constructed into transcriptome libraries using NEBNext Single Cell/Low Input RNA Library Prep Kit (New England BioLabs) and sequenced using NextSeq2000 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... Approximately 5-10 ng of input RNA was used to produce libraries with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) which were sequenced on an Illumina HiSeq 3000 to produce 50 bp single-end reads ...
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bioRxiv - Immunology 2024Quote: ... 10ng RNA was used to prepare libraries using Single Cell/Low Input RNA Library Prep Kit for Illumina (New England BioLabs). Libraries with different indexes were pooled and sequenced by an Illumina NovaSeq 6000 as paired-end reads extending 150 bases.
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bioRxiv - Neuroscience 2024Quote: ... RNA input was normalized and NGS libraries were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and remaining enzymes and master mixes were ordered from New England Biosciences (NEB, USA). Plasmids were routinely isolated using the Qiaprep Spin Miniprep kit (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... The doubly-digested vectors were assembled with a single fragment containing the ORF containing 5’ and 3’ adapters for Gibson assembly using 2x NEB Hifi Mastermix (NEB, Ipswich, MA). The finished constructs were transformed into BL21 Rosetta (DE3 ...
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bioRxiv - Microbiology 2021Quote: ... the purified cDNA was PCR amplified and barcoded using ONT’s PCR Barcoding Expansion 1-12 (EXP-PBC001) or PCR Barcoding Expansion 1-96 (EXP-PBC096) kits with LongAmp Taq 2x Mastermix (NEB, Ipswich, MA).
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bioRxiv - Plant Biology 2022Quote: ... The doubly digested vectors were assembled with a single fragment containing the ORF containing 5′ and 3′ adapters for Gibson assembly using 2x NEB Hifi Mastermix (NEB, Ipswich, MA). The finished constructs were transformed into BL21 Rosetta (DE3 ...
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bioRxiv - Microbiology 2022Quote: Single-stranded cDNA was used as a template for PCR amplification to amplify all eight genes using segment specific primers using high-fidelity Phusion 2X DNA polymerase (New England BioLabs, Inc., USA). Primer sequences are available in the GitHub repository accompanying this manuscript 33 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The purified fragments were treated with KLD enzyme mix (New England Biolabs) and transformed into E ...
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bioRxiv - Molecular Biology 2020Quote: ... 70 μl of end-repair mix was added (1× Ligation buffer (NEB), 357 μM dNTPs ...
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bioRxiv - Genomics 2019Quote: ... For that the mix containing 2 µL of RNAse H (NEB, #M0297S), 1 µL of E ...
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bioRxiv - Developmental Biology 2020Quote: ... The ligation mix was transformed using NEB stable competent cells (NEB, C3040) for single colony isolation ...
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bioRxiv - Cancer Biology 2021Quote: ... A mix of 5 µL of 10X NEB2.1 buffer (New England Biolabs), 1.25 µL of 1 mM dATP ...
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bioRxiv - Systems Biology 2020Quote: ... Nick repair was performed by using PreCR Repair Mix (New England Biolabs). Lambda exonuclease- and RecJf exonuclease-treated chromatin was eluted from the beads and overnight incubation at 65 °C reversed the protein-DNA cross-link ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl of 10-mM deoxynucleoside triphosphate mix (NEB, Ipswich, Massachusetts, USA), 1 μl of each 25-μM primer ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR product was then added to a KLD enzyme mix (NEB) reaction and was incubated at room temperature for 1 hour ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... Nick repair was performed by using PreCR Repair Mix (New England Biolabs). Lambda exonuclease- and RecJf exonuclease-treated chromatin was eluted from the beads and overnight incubation at 65 °C reversed the protein-DNA cross-link ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM CTP and 0.6 μl T7 polymerase mix (New England Biolabs.) This target mix was then loaded on to the chip by placing 10 μL of the mix on the sticky side of the PCR film (MicroAmp ...
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bioRxiv - Immunology 2021Quote: ... and the fragment was assembled using Hi-Fi DNA Assembly Mix (NEB) into the donor vector pADN171XS7 ...
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bioRxiv - Molecular Biology 2020Quote: ... a mix of 4 μl First strand Synthesis Reaction Buffer (NEB-kit), 0.5 μl Murine RNase Inhibitor (NEB-kit ...
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bioRxiv - Synthetic Biology 2021Quote: ... using NEBuilder® HiFi DNA Assembly Mix (New England Biolabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Nick repair was performed by using PreCR Repair Mix (New England Biolabs). Lambda exonuclease-and RecJf exonuclease-treated chromatin was eluted from the beads and overnight incubation at 65 °C reversed the protein-DNA cross-link ...
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bioRxiv - Genomics 2022Quote: ... 500 nL of 5hmC-blocking mix (1 U T4-BGT (NEB, M0357L), 6x UDP-glucose ...
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bioRxiv - Bioengineering 2020Quote: ... using NEBuilder HiFi DNA Assembly Mater Mix (New England BioLabs, Ipswich, MA). The 202 bp and 266 bp promoters were cloned and amplified with specific primers (Suppl ...
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bioRxiv - Developmental Biology 2022Quote: ... we reverse-transcribed cDNA with a random primer mix (NEB, MA, USA) and GoScript™ Reverse Transcriptase (Promega ...
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bioRxiv - Systems Biology 2019Quote: ... Nick repair was performed by using PreCR Repair Mix (New England Biolabs). Lambda exonuclease- and RecJf exonuclease-treated chromatin was eluted from the beads and overnight incubation at 65°C reversed the protein-DNA cross-link ...
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bioRxiv - Molecular Biology 2019Quote: ... 10–20% were sequentially digested with a mix of restriction enzymes (NEB) according to their buffer requirements and temperatures ...
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bioRxiv - Synthetic Biology 2020Quote: ... and assembled using the Gibson Assembly mix (New England Biolabs, Ipswich, MA). pWHE601-C45 was then used as template to generate an amplicon that includes one cassette and C45 using ADO17 and ADO18 primers ...
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bioRxiv - Immunology 2019Quote: ... PCR products and vector were ligated using high fidelity assembly mix (NEB) into expression plasmids adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK2 vectors (Invivogen) ...
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bioRxiv - Genomics 2019Quote: ... DNA repair was performed using NEBNext FFPE DNA Repair Mix (M6630, NEB). End-prep was performed using NEBNext Ultra II End Repair/dA-Tailing Module (E7546L ...
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bioRxiv - Genetics 2021Quote: ... a 30 µl mix with 1.6 µl of T4 DNA ligase (NEB) was added to each well and placed on a thermocycler (16°C 60 min followed by 80°C 30 min for enzyme inactivation) ...
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bioRxiv - Genetics 2021Quote: ... DNA repair was performed using NEBNext FFPE DNA Repair Mix (M6630L, NEB). End repair was performed using NEBNext Ultra II End Repair/dA-Tailing Module (E7546L ...
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bioRxiv - Bioengineering 2020Quote: ... Single amino acid substitutions were introduced utilizing the KLD Enzyme Mix (NEB) following PCR amplification with mutagenic primers (Genewiz) ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM CTP and 0.6 μl T7 polymerase mix (New England Biolabs)