Labshake search
Citations for New England Biolabs :
4201 - 4250 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... DNA was repaired (FFPE DNA Repair Mix, New England BioLabs® (NEB)) ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 3 µl Ultra II End-prep enzyme mix (New England Biolabs). Samples were incubated at 20°C for 5 minutes ...
-
bioRxiv - Immunology 2020Quote: ... or Ig Lambda using Gibson assembly enzyme mix (New England Biolabs #E2621L) after confirming paired amplified product on 96-well E gel (ThermoFisher #G720801) ...
-
bioRxiv - Genomics 2021Quote: ... 0.5 μL of 10 mM Deoxynucleotide (dNTP) solution mix (New England Biolabs) was added to each reaction and nuclease -free water was used to make up the remaining reaction volume ...
-
bioRxiv - Genomics 2019Quote: ... or 5μL NEB RNase H reaction mix (10U NEB RNase H [NEB M0297S] and 1uL 10X NEB RNase H reaction buffer ...
-
bioRxiv - Bioengineering 2022Quote: ... 1X of a restriction enzyme mix [1/2 EcoRI-HF® (NEB #R3101 ...
-
bioRxiv - Developmental Biology 2022Quote: ... while the other was treated overnight with Protein Deglycosylation Mix II (NEB) using non-denaturing reaction conditions according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... All PTet plasmids were generated using Gibson reactions (NEB HiFi Assembly Mix) from PCR products ...
-
bioRxiv - Cell Biology 2022Quote: ... three volumes of ligation mix [1.33× T4 DNA ligase buffer (B0202, NEB), 1.11% Triton X-100 ...
-
bioRxiv - Cell Biology 2022Quote: ... and Illumina adaptor ligation mix [0.075x End repair reaction buffer (E6050, NEB), 0.32× Ligation master mix (E7595 ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA fragments were first treated with End repair enzyme mix (NEB, E5060) for 30 min at 20°C in 50μl volume ...
-
bioRxiv - Microbiology 2022Quote: ... and 3 μL of Ultra-prep enzyme mix (New England Biolabs, UK), and completed with molecular biology grade water ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 3 μl NEBNext Ultra II End Prep Enzyme Mix (NEB, E7546), incubated for 10 min at room temperature followed by 10 min at 65 °C ...
-
bioRxiv - Genetics 2022Quote: ... The ligation mix was transformed using NEB stable competent cells (NEB, C3040). Correct insertion of sgRNA sequence was confirmed by Sanger sequencing.
-
bioRxiv - Genomics 2022Quote: ... reactions were incubated in ExoDigest Mix (100U Exonuclease III (New England Biolabs), 1x NEBuffer ...
-
bioRxiv - Genomics 2024Quote: ... and 1X BsmBI-v2 Golden Gate Enzyme Mix (New England Biolabs E1602S) with the thermal cycling protocol ...
-
bioRxiv - Microbiology 2024Quote: ... and ligated using Instant Ligase Sticky-end Ligase Mix (New England Biolabs). Ligations were transformed into NEB10β competent cells following the manufacturer recommendations (New England Biolabs) ...
-
bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized using a random primer mix (New England Biolabs, S1330S) and ProtoScript® II reverse transcriptase (New England Biolabs ...
-
bioRxiv - Microbiology 2023Quote: ... Point mutations were introduced using the KLD Enzyme mix (New England Biolabs) (Table S7 ...
-
bioRxiv - Bioengineering 2022Quote: ... and inserted into the backbone using HiFi cloning mix (New England Biolabs). All constructs were verified with Sanger sequencing ...
-
bioRxiv - Microbiology 2022Quote: ... 2 μL of NEBNext FFPE DNA Repair Mix (New England Biolabs, UK), 3.5 μL Ultra II End-prep reaction buffer ...
-
bioRxiv - Biophysics 2023Quote: ... We performed a BsaI Golden Gate assembly (NEB, Golden Gate Assembly Mix) in T4 DNA Ligase Buffer in 20 µL volumes using equimolar pDT2 and the Cy5/biotin duplex at a final concentration of 6 nM each ...
-
bioRxiv - Biochemistry 2023Quote: ... The reactions were treated by Nucleoside Digestion Mix (New England Biolabs, Inc.) to release the nucleosides and the dinucleotide cap structure (m7GpppG or m7GpppA) ...
-
bioRxiv - Bioengineering 2023Quote: ... The PCR products were treated with a KLD enzyme mix (NEB M0554S), which phosphorylates the 5’ ends of the linearized PCR products ...
-
bioRxiv - Neuroscience 2023Quote: ... and 1 µL of Template Switching RT Enzyme Mix (NEB Catalog# M0466L). After mixing ...
-
bioRxiv - Immunology 2023Quote: ... The PCR product was phosphorylated and ligated with KLD enzyme mix (NEB) before transformation into competent cells followed by sequence verification (1st BASE ...
-
bioRxiv - Genomics 2023Quote: ... 10 μL of Digestion-3 mix (5 U NotI-HF (NEB, R3189L) in 1X CutSmart buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 1 μL of 10 mM dNTP mix (NEB N0447S, Ipswich, MA). This initial premix was heated at 65°C for 5 minutes ...
-
bioRxiv - Genomics 2023Quote: ... 1 μl 10 mM mix of dGTP and dTTP (NEB #N0442S, #N0443S), 5 μl 10x T4 DNA Ligase Buffer ...
-
bioRxiv - Plant Biology 2023Quote: ... Adapter Mix II (ONT, Oxford, UK) and Quick T4 DNA Ligase (NEB) were added and incubated for 10 minutes at room temperature and purified with 0.5X AMPure XP beads (Beckman Coulter) ...
-
bioRxiv - Microbiology 2023Quote: ... then insertion gene fragments with NEBuilder HIFI DNA Assembly Mix (NEB, E5520S). For transfection and replication ...
-
bioRxiv - Molecular Biology 2023Quote: ... All assembly reactions were done using NEBuilder HiFi DNA assembly mix (NEB). The resulting plasmid ...
-
bioRxiv - Molecular Biology 2023Quote: ... All assembly reactions were done using NEBuilder HiFi DNA assembly mix (NEB). The constructs were digested with XhoI and introduced into the BTU1 locus of the new MAC of conjugating two SPT6 KO homozygous heterokaryon strains at 10 hpm by electroporation ...
-
bioRxiv - Plant Biology 2023Quote: ... linearized pEAQ-HT vector27 using HiFi DNA assembly mix (New England BioLabs). Constructs were transformed into 10-beta competent E ...
-
bioRxiv - Genomics 2024Quote: Mix gently and add 5 ul of 20mg/ml proteinase K (NEB) solution to each tube and incubate for 3 hr at 45°C being careful there is no condensation.
-
Tuning the performance of a TphR-based terephthalate biosensor with a design of experiments approachbioRxiv - Synthetic Biology 2024Quote: ... and plasmid assembly was performed using NEBuilder HiFi DNA Assembly Mix (NEB), followed by transformation into chemically competent E ...
-
bioRxiv - Systems Biology 2024Quote: ... and 3 μL of NEBNext Ultra II End Prep Enzyme Mix (NEB) were added to each sample and mixed well by pipetting up and down ...
-
bioRxiv - Biochemistry 2020Quote: ... beads were resuspended in 15 μL low salt buffer and the DNA was released by adding 600 units of Micrococcal nuclease (NEB M0247S) for 5 minutes with shaking (1250 rpm ...
-
bioRxiv - Microbiology 2020Quote: ... was subjected to first strand and second strand cDNA synthesis followed by adaptor ligation and enrichment with a low-cycle according to the instructions of NEBNext” UltraTM RNA Library Prep Kit for Illumina (NEB, USA). The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit”2.0 (Life Technologies ...
-
bioRxiv - Cancer Biology 2021Quote: Libraries were prepared using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs). Quality control and quantification of the libraries were performed using the Bioanalyzer DNA 1000 kit (Agilent ...
-
bioRxiv - Neuroscience 2022Quote: ... 100 sorted cells were collected into individual wells of 96-well plate containing 5 μl of lysis buffer of NEB Next single-cell low input RNA library prep kit for Illumina (New England Biolabs #E6420). Plates were frozen immediately on dry ice and stored at −80 °C ...
-
bioRxiv - Bioengineering 2022Quote: ... The samples were run in a 5% native PAGE gel in an ice bath along with low range ssRNA ladder (New England Biolabs, Inc.). The gels were prepared using Acrylamide ...
-
bioRxiv - Immunology 2019Quote: ... and libraries were prepared from 2 ng of total RNA using the NEBNext low input kit (New England Biolabs, Hitchen, U.K.). Libraries were assessed for correct size distribution on the Agilent 2200 TapeStation and quantified by Qubit DNA High Sensitivity assay (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA libraries were prepared from total RNA samples with an RIN ≥ 8.0 using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England BioLabs). An Illumina NovaSeq 6000 machine with an S4 flow cell was used to generate pairwise 100 bp reads (performed by the Yale Center for Genome Analysis).
-
bioRxiv - Microbiology 2021Quote: ... followed by adaptor ligation and enrichment with a low-cycle according to the instructions of NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA). The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit2.0 (Life Technologies ...
-
bioRxiv - Cell Biology 2022Quote: ... Sequencing libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs #E6420) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2022Quote: ... were used for RNAseq library preparation with the NEBNext Single Cell / Low Input RNA Library Prep Kit for Illumina (NEB, E6420S), following the manufacturer’s protocol for single cells ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA-seq libraries were prepared with 10 ng of total RNA using NEBNext® single cell/low input RNA library preparation Kit (New England Biolabs). Single-end sequencing was performed on all the samples with a 75 bp sequencing depth on a NextSeq machine at the RNomics platform of the Université de Sherbrooke (Canada) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and eluted in 10 μL of Ultra Pure™ DNase/RNase free distilled water: the purified amplicon and the low molecular weight DNA ladder (NEB) were run on 2% agarose gel in 1x TBE for 1 h 40 min at 100 V ...
-
bioRxiv - Molecular Biology 2022Quote: ... Full-length cDNA was synthesized and amplified using the NEBNext® Single Cell/Low Input cDNA Synthesis and Amplification Module (New England Biolabs) and the Iso-Seq Express Oligo Kit (Pacific Biosciences) ...