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Citations for New England Biolabs :
4101 - 4150 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were then amplified with Q5 PCR mix (New England Biolabs) for a total of 16-25 cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... cell lysates were first incubated with Deglycosylation Mix Buffer 2 (NEB) at 75°C for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... digested to nucleosides with the Nucleoside Digestion Mix (NEB, Ipswich MA) and the reaction products were quantified with LC-MS/MS.
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bioRxiv - Bioengineering 2023Quote: ... 200 uM of deoxynucleotide (dNTP) solution mix (New England Biolabs (NEB), cat# N0447L) ...
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bioRxiv - Biophysics 2023Quote: ... and added to 10 µl of 2xHiFi mix (New England Biolabs). We incubated the reaction at 50°C for 60 min ...
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bioRxiv - Bioengineering 2023Quote: ... 200 uM of deoxynucleotide (dNTP) solution mix (New England Biolabs (NEB), cat# N0447L) ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL ligation mix (2 µL 10x T4 ligase buffer, NEB, 1 µL T4 RNA ligase 2 ...
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bioRxiv - Genomics 2023Quote: ... 5 μL of Digestion-2 mix (NdeI (5 U, NEB, R0111L) and/or BglII (5 U ...
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bioRxiv - Genomics 2023Quote: ... 2.5 μL of Digestion-1 mix (MseI (12.5 U, NEB, R0525M) and/ or MboI (12.5 U ...
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bioRxiv - Immunology 2023Quote: ... and amplified with NEBNext High Fidelity PCR Mix (New England Biolabs). Library quality was assessed using a TapeStation instrument ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.96 μL of 25 mM rNTP mix (NEB N0466S, Ipswich, MA), 0.12 μL of 100 μM 6U FAM reporter (IDT) ...
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bioRxiv - Cancer Biology 2023Quote: ... For nucleosomes labeling reaction mix contained: NEBuffer™ 2 (NEB B7202), protease and HDAC inhibitors (as detailed above) ...
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bioRxiv - Molecular Biology 2023Quote: ... the ligation mix with 20 U T4 RNA Ligase buffer (NEB) was added and incubated for 3 hours at 25 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µl of Golden Gate Enzyme Mix (BsaI-HFv2, NEB M2616AA), and adding dH2O to reach a total volume of 20 µl ...
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bioRxiv - Synthetic Biology 2024Quote: ... interORF and ORF2p were then assembled using Hifi Assembly mix (NEB) into pCMV vector with the CMV promoter region removed ...
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bioRxiv - Molecular Biology 2024Quote: ... and NEBNext Ultra II End Prep Enzyme Mix (New England Biolabs) at 20°C for 30 minutes followed by 65°C for 30 minutes ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Final concentration was measured by qPCR using the NEBNext Library Quant Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... further verified by qPCR using NEBNext Library Quant Kit for Illumina (New England Biolabs #E7630S) on Stratagene Mx3000P instrument and its profile analysed by Agilent Tape station 2200 ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... using the Luna Universal One-Step RT-qPCR Kit (New England BioLabs, #E3005L or #E3006E) according to manufacturer’s instructions and then analyzed with QuantStudio Design & Analysis Software v1.5.1 (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... The recovered DNA was analyzed by quantitative real-time PCR using LunaScript qPCR mastermix (NEB) and the ABI StepOnePlus qPCR machine and primers in Table 3 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX384 (Bio-Rad).
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bioRxiv - Biophysics 2021Quote: ... We used the Luna Universal One-step RT-qPCR kit (E3005S, New England Biolabs, MA) to determine the relative abundance of target mRNA between two samples ...
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bioRxiv - Molecular Biology 2021Quote: ... Common amplicon libraries were quantified with qPCR using NEBNext Library Quant kit (New England Biolabs) and pooled to equal concentrations ...
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bioRxiv - Microbiology 2020Quote: ... using Luna Universal Probe One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA concentrations were quantified by qPCR with the NEBNext Library Quant Kit for Illumina (NEB). Libraries were pooled and sequenced for 28-90 paired-end nucleotide reads to an average depth of ∼70,000 mapped fragments per cell.
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bioRxiv - Immunology 2022Quote: ... Libraries were quantified by qPCR using the NEBNext Library Quant kit for Illumina (NewEngland Biolabs) according to manufacturer’s protocol and pooled at equal molarity (1nM) ...
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bioRxiv - Microbiology 2022Quote: ... and 16s rRNA transcripts by the Luna Universal One-Step RT-qPCR kit (NEB, E3005), according to the manufacturer’s instructions on an Applied Biosystems 7500 Fast Real-Time PCR System ...
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bioRxiv - Biochemistry 2023Quote: ... For assessment of HAC1spliced mRNA the Luna Universal Probe One-Step RT-qPCR Kit (NEB) was used with 2 µl of a 50 ng/ µl RNA sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were run using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX384 (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, USA) and a BIORAD CFX Opus 384 system ...
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bioRxiv - Genomics 2021Quote: PacBio Iso-Seq templates were prepared using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England BioLabs) and PacBio Iso-Seq Express Template Switching Oligos (TSO ...
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bioRxiv - Molecular Biology 2021Quote: ... Library preparation from human islets total RNA (200ng) was performed using a NEBNext Low Input RNA Library Prep kit (NEB). Sequencing was performed on a HiSeq4000 using 75 bp paired end reads according to Illumina specifications ...
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bioRxiv - Genomics 2019Quote: ... The 5 µL of each PCR cycle sample along with the 20 µL digested sample, and titration of the low molecular ladder (100 ng, 200 ng, 400 ng) (NEB) were run on a 2% Agarose gel ...
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bioRxiv - Genomics 2019Quote: ... Ligated RNA was enriched with biotin-labeled products by another round of Streptavidin bead binding and washing (two washes each of High, Binding and Low salt buffers and one wash of 1x Thermo Pol Buffer (NEB)) ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA libraries were made from RNA using NEB’s Next Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB E6420). Libraries were sequenced on an Illumina HiSeq 4000 with single reads of 50 bases.
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bioRxiv - Biochemistry 2021Quote: ... The floated fraction corresponding to a mixture of high- and low-density EVs (42) was treated with MNase (NEB, M0247S) to degrade any nucleic acid not contained within the EVs and deactivated with 25 mM ethylene glycol-bis(β-aminoethyl either)-N,N,N′,N′-tetraacetic acid (EGTA ...
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bioRxiv - Synthetic Biology 2021Quote: ... gel run at 120 V for 45 min by comparing with a dsDNA ladder (Low Molecular Weight DNA Ladder, NEB).
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bioRxiv - Plant Biology 2020Quote: GBS libraries for the low-resolution mapping were prepared from genomic DNA digested with PstI and MspI (New England Biolabs) as described by Wendler et al ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was ligated to a low-copy plasmid vector pWSK29 (BamHI/Sall) by Gibson assembly (New England Biolabs). Similarly ...
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bioRxiv - Neuroscience 2020Quote: ... a gel slice corresponding to 60 to 80 nt was isolated (based on size markers in an adjacent lane: low range ssRNA ladder, NEB). The reverse transcribed product in the gel slice was eluted ...
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bioRxiv - Neuroscience 2020Quote: ... a gel slice corresponding to 80 to 150 nt was isolated (based on size markers in an adjacent lane: low range ssRNA ladder, NEB). The reverse transcribed product in the gel slice was eluted ...
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bioRxiv - Neuroscience 2021Quote: ... Strand-specific RNASeq libraries were created using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB #6420L ...
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bioRxiv - Neuroscience 2021Quote: ... Products were identified with gel electrophoresis using a molecular weight marker (Low molecular weight marker, New England Biolabs, Frankfurt, Germany). As a positive control ...
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bioRxiv - Neuroscience 2020Quote: ... The size of libraries was selected for cDNA target fragments of 200–300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 cycles ...
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bioRxiv - Cell Biology 2021Quote: Plate-based scRNA-seq was performed with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (catalog no. E6420L; New England Biolabs). Total cell fractions from dissociated gut sections of donors BRC2033-2034 ...
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bioRxiv - Genetics 2020Quote: ... three selected guide RNAs based on their high Guide Design Tool scores and low off-target probabilities were assessed by the T7 endonuclease I (NEB) assay per manufacturer’s direction ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then transferred to a fresh low-bind tube containing pre-washed 5 µl amylose magnetic beads (NEB) and rotated for 45 min at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... The products were analyzed by electrophoresis in a non-denaturing 12% polyacrylamide gel against a 5’-labeled Low Molecular Weight DNA Ladder (New England Biolabs) and quantitated with a phosphorimager (Typhoon FLA 9500 ...
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bioRxiv - Molecular Biology 2022Quote: ... and analyzed by electrophoresis in a non-denaturing 12% polyacrylamide gel with 5’-labeled Low Molecular Weight DNA Ladder (New England Biolabs) run in a parallel lane ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μl of the Phire PCR reaction was verified on a 2% agarose gel with a low molecular weight ladder (N3233S, NEB). 15 μl of PCR products were pooled and purified using PCR Purification Kit (D4013 ...