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Citations for New England Biolabs :
3951 - 4000 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Tagmented samples were amplified by the addition of 2.5μL each of 10μM barcoded forward and reverse primers (Picelli et al., 2014) and 15μL Q5 2x HiFI MasterMix (New England Biolabs) using the following thermocycling conditions ...
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bioRxiv - Microbiology 2020Quote: ... The resulting backbone was subsequently gel-purified and assembled with the gBlocks fragments using a 2x Gibson mastermix (NEB). Gibson assembly was possible due to a 23 bp sequence shared between the KpnI-XbaI-cut pB1H2-w2 backbone and the gBlocks fragment ...
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bioRxiv - Molecular Biology 2023Quote: ... Illumina Truseq adaptors were ligated to the DNA fragments by adding 25μl of 2X Quick Ligase buffer (NEB, M2200), 1μl of adaptors (1μl of a 1:250 dilution of Illumina stock solution) ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmid DNA assembly of landing pad constructs was completed using NEBuilder HiFi 2X Mastermix in accordance with the manufacturer’s protocol (New England Biolabs), as well as Primerize for attB and attP site assembly ...
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bioRxiv - Molecular Biology 2024Quote: ... the beads were resuspended in 2X B&W buffer supplemented with 0.4 U/µL RNase Inhibitor (Murine) (NEB, M0314S) to a bead concentration of 5 ug/µL ...
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bioRxiv - Immunology 2021Quote: ... Luna Universal Probe One-step RT-qPCR kit (New England Biolabs, Ipswich, MA) was used to amplify the envelope (E ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was quantified by qPCR using NEBNext universal and index primers (NEB E7335S). Libraries were indexed using NEBNext High-Fidelity PCR Master Mix (NEB M0541S ...
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bioRxiv - Molecular Biology 2020Quote: ... and quantified by qPCR using NEBNext Library Quant kit for Illumina (NEB, E7630). Equal molarity of each library were pooled and subjected to sequencing on an Illumina HiSeq 4000 platform at the McGill University and Génome Québec Innovation Centre to generate 50 bp paired-end reads.
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bioRxiv - Immunology 2020Quote: ... we used the Luna Universal Probe Onestep RT-qPCR kit (New England Biolabs) with 1 ug of RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... The Luna Universal Probe One-Step RT-qPCR Kit (Cat. No. E3006S, NEB) was used as the relevant master mix ...
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bioRxiv - Evolutionary Biology 2022Quote: ... using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs). mcr-1 and trfA (reference ...
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bioRxiv - Plant Biology 2022Quote: ... 5 µL reaction volume contained 2.5 µL Luna® Universal qPCR mastermix (NEB). RT-qPCR analyses were performed using the Real-time PCR Roche Lightcycler 480 and the expression of PP2AA3 (At1G13320 ...
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bioRxiv - Microbiology 2022Quote: ... the NEB Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) was used and the qPCR was conducted on the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific)44 ...
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bioRxiv - Microbiology 2023Quote: ... The Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... For these analyses Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX opus real-time 384 system (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... After quantification by qPCR using the NEBnext library quant kit for Illumina (NEB), pooled libraries were sequenced on an Illumina HiSeq 2500 platform using a 50 bp single read on a high output standard v4 flow cell with a 10–20% PhiX spike-in ...
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bioRxiv - Bioengineering 2023Quote: The Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs, USA) was used ...
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bioRxiv - Cell Biology 2019Quote: ... cells were labeled with Oregon-Green SNAP substrate (New England Biolabs) at 4 µM final concentration for labeling of the newly synthesized CENP-A molecules ...
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bioRxiv - Cell Biology 2022Quote: ... SNAP-Cell Oregon Green (New England Biolabs Inc., Boston, MA, USA) was used to label and visualize Cx36 expressing cells ...
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bioRxiv - Neuroscience 2022Quote: ... qRT-PCR reactions (25μL) contained 12.5μL of Sybr Select or Luna (NEB) Mastermix and 0.25 μM primers ...
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bioRxiv - Microbiology 2021Quote: ... 8 μL of RNA was added per section to step 2.1 through to 2.11.11 of the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... we used either a DNA ladder (Low Molecular Weight DNA Ladder or 1 kb Plus DNA Ladder, NEB) or an RNA ladder (RiboRuler Low Range or High Range RNA Ladder ...
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bioRxiv - Molecular Biology 2022Quote: Low-yield IVT reaction conditions were carried out with 70 nM T7 RNA polymerase (M0251, New England Biolabs), 20 mM or 10 mM rNTP ...
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bioRxiv - Genomics 2020Quote: ... cDNA was reverse transcribed using the NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module (NEB E6421S) from 238 ng total RNA ...
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bioRxiv - Systems Biology 2022Quote: ... cDNA was synthesized using the NEB Single Cell/Low Input cDNA Synthesis and Amplification Module (New England Biolabs).
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bioRxiv - Cancer Biology 2020Quote: ... Library preparation for whole genome sequencing used enzymatic fragmentation and the NEBNext Ultra II low input kit (NEB) with 150bp paired end on either Illumina HiSeqX or Novaseq machines ...
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bioRxiv - Evolutionary Biology 2021Quote: ... putative methyltransferases were cloned into the BamHI/SalI sites of the low copy vector pACYC184 (New England Biolabs) with an upstream Shine-Dalgarno consensus sequence (5’-AGGAGG-3’) ...
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bioRxiv - Immunology 2022Quote: ... Library preparation and sequencing was completed using NEBNext® Single Cell/Low Input RNA Library Prep Kit (NEB) and NextSeq 550 (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... A mixture containing 1 μl of a 4-fold dilution of Low Range ssRNA Ladder (New England Biolabs) and 0.75 pmol each of RPIX_SC1_Bridge ...
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bioRxiv - Cell Biology 2024Quote: ... RNA-seq libraries were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB). Pooled libraries were sequenced with paired end sequencing on a Novoseq 6000 by Novogene.
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were generated using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2023Quote: ... The NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module kit (New England BioLabs Inc. MA, USA) was used following manufacturers protocols for cDNA synthesis and amplification from 300 ng of RNA ...
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bioRxiv - Microbiology 2024Quote: ... The library preparation was elaborated using the NEBNext® SingleCell/Low Input cDNA Synthesis kit (New England Biolabs). The prepared libraries were then sequenced ...
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bioRxiv - Biochemistry 2021Quote: ... according to the LAMP method described earlier with additions to the earlier described CRISPR Mix (Modification: 1 μL NTP Buffer Mix (From NEB #E2050, no additional MgCl).
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bioRxiv - Evolutionary Biology 2021Quote: ... and 30 μl Phusion DNA polymerase mix (New England Biolabs). The reaction was incubated at 72°C 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... a mix containing 2 μl of RNAse H (NEB, #M0297S), 1 μl of E ...
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bioRxiv - Genomics 2020Quote: ... and amplicons were repaired with NEBNext FFPE Repair Mix (NEB), followed by the DNA ends preparation using NEBNext End repair/ dA-tailing Module (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... and then incubated with restriction enzyme mix (HindIII (NEB, R01045), EcoRI (ThermoFisher ...
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bioRxiv - Genomics 2019Quote: ... This duplex was purified and assembled using Gibson mix (NEB) with p426-crRNA-CAN1.Y that had been cut with NheI and Acc65I to replace the CAN1.Y guide target region with a stuffer fragment that could be released by BaeI (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... using Gibson Assembly reagent mix (New England Biolabs; Beverley, MA) [69] ...
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bioRxiv - Genomics 2021Quote: ... 50 μL of Q5 DNA polymerase reaction mix (NEB, M0491S) was used ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1.5 μl of 3’Ligation Enzyme Mix (NEB-kit) were added and incubated for 18 h at 16°C over-night ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1.25 μl of 5’Ligation Enzyme Mix (NEB-kit) followed by a 1 h incubation at 25°C ...
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bioRxiv - Plant Biology 2022Quote: Nucleic acids were digested using the Nucleoside Digestion Mix (NEB) with the following modifications ...
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bioRxiv - Molecular Biology 2020Quote: ... 1μl of Luna WarmStart RT Enzyme Mix (New England Biolabs) and 3.9 μl water ...
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bioRxiv - Genomics 2022Quote: ... Each reaction contained 10 µL of Q5 Reaction Mix (NEB), 10 µL of Q5 GC Enhancer Buffer (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... using NEB HiFi Assembly Mix (New England Biolabs, Ipswitch, MA). All primers and synthetic DNAs for this study are shown in Table 1.
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bioRxiv - Genomics 2019Quote: ... and nick-repaired with NEBNext FFPE DNA Repair Mix (NEB). Subsequently ...
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bioRxiv - Genomics 2019Quote: ... the nick-repaired DNA (NEBNext FFPE DNA Repair Mix, NEB) was sheared in a Covaris g-TUBE (Covaris ...
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bioRxiv - Genomics 2019Quote: ... 2 µl of NEBNext FFPE DNA Repair Mix (M6630, NEB), 3.5 µl of NEBNext Ultra II End Prep Reaction Buffer ...