Labshake search
Citations for New England Biolabs :
4251 - 4300 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... or a low input method using NEB Next® Ultra RNA Library Prep Kit for Illumina® (NEB, Cat. No. 7530). First-strand cDNA synthesis and tailing by reverse transcription were performed using SMART (Switching Mechanism at 5’ End of RNA Template ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... Libraries were prepared for both the INPUT and IP RNA using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina) (NEB, #E6420S) following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... NEBNext Low Input RNA libraries were prepared manually following the manufacturer’s protocol (NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® Instruction Manual Version 5.0_5/20, NEB #E6420L). Samples were normalized to 1ng total RNA material per library in 8μl of nuclease-free water ...
-
bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized and amplified using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, Ipswich, MA), Iso-Seq Express Oligo Kit (Pacific Biosciences ...
-
bioRxiv - Genomics 2023Quote: ... PacBio Iso-Seq libraries were prepared using the NEBNext® Single Cell/Low Input cDNA Synthesis and Amplification Module (New England BioLabs) and the SMRTbell Express Template Prep Kit 2.0 following standard protocol and sequenced on the PacBio Sequel II at the University of Queensland Sequencing Facility.
-
bioRxiv - Neuroscience 2023Quote: ... RNA libraries were prepared using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs) and sequenced using the Illumina NOVAseqSP sequencer.
-
bioRxiv - Evolutionary Biology 2023Quote: RNA libraries from at least three replicate individuals per sex for each lineage (Table S1) were prepared from gametes using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs). Mature gametophytes release gametes just after the medium renewal ...
-
Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... prior to mRNA library preparation using the Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs). Libraries were sequenced on an Illumina HiSeq 2500 V4 system ...
-
bioRxiv - Cell Biology 2023Quote: ... RNA from Luminal mature and progenitor cells was amplified using the “NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification” (NEB). For RNA-Seq 20,0000 to 50,000 sorted basal and luminal cells from the shTrps1 ubiquitous mouse model (Fig S4 ...
-
bioRxiv - Developmental Biology 2023Quote: Libraries for RNA sequencing were generated using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB #E6420) in conjunction with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
-
bioRxiv - Bioengineering 2024Quote: Samples were resuspended in 5 µl 1X NEBNext Cell Lysis Buffer of the NEBNext Single Cell/Low Input RNA Library Prep Kit (NEB, E6420). After 5 min incubation at RT ...
-
bioRxiv - Genomics 2024Quote: ... Supplementary Table 1) using a modified protocol of the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, UK) (Supplementary Methods and Supplementary Table 18 ...
-
bioRxiv - Immunology 2022Quote: ... Particle titers were determined by isolation of genomic RNA and quantitation by RT-qPCR (NEB # E3031) using an Applied Biosystems vii7A™ Real-Time PCR system ...
-
bioRxiv - Biochemistry 2020Quote: ... The samples were prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Quantitative RT-PCR was performed with an Mx3000P qPCR system (Agilent ...
-
bioRxiv - Microbiology 2020Quote: ... The indexed libraries were quantified by qPCR using the NEBNext Library Quant Kit for Illumia (NEB, mixed and sequenced on a MiSeq instrument (Illumina) ...
-
bioRxiv - Plant Biology 2021Quote: ... Real-time quantitative PCR was performed on cDNA with Luna Universal qPCR Mastermix (New England Biolabs) in a CFX384 thermal cycler (Bio-Rad) ...
-
bioRxiv - Pathology 2022Quote: ... qRT-PCR analysis was carried out using the Luna Universal One-Step RT-qPCR kit (NEB) using 100 ng RNA as input according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: RNA for qPCR was extracted using the Monarch Total RNA Miniprep kit (T2010, New England Biolabs), and reverse transcription performed using LunaScript RT SuperMix (E3010 ...
-
bioRxiv - Molecular Biology 2019Quote: ... The final pool was quantified by qPCR with the NEBNext Library Quant Kit for Illumina (NEB) and sequenced paired-end (PE ...
-
bioRxiv - Genomics 2021Quote: ... One-step qRT-PCR was performed using the Luna Universal One-Step RT-qPCR Kit (NEB) and 5 ng RNA per reaction in technical duplicate with a StepOnePlus Real-Time PCR system (Applied Biosystems ...
-
bioRxiv - Synthetic Biology 2024Quote: ... library concentrations were quantified with qPCR using the NEBNext Library Quant Kit for Illumina (NEB, E7630S), and pre- and post-selection libraries were combined as two pools ...
-
bioRxiv - Molecular Biology 2023Quote: ... The samples were prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Quantitative RT-PCR was performed with an Mx3000P qPCR system (Agilent ...
-
bioRxiv - Molecular Biology 2024Quote: ... Quantitative RT-PCR was conducted with the Luna Universal One-Step RT-qPCR Kit (NEB, E3005) on a Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and neomycin resistance were PCR-amplified from extant constructs present in our laboratory using NEB Q5 Hot-Start 2X Mastermix according to the manufacturer’s protocol (New England Biolabs, Ipswitch, MA, USA; M0494L). HDR-donor assemblies were completed using NEBuilder HiFi 2X Mastermix in accordance with the manufacturer’s protocol (New England Biolabs ...
-
bioRxiv - Cancer Biology 2023Quote: ... Washed Dynabeads containing the DNA-complexes were directly resuspended in 20 µL USER reaction buffer containing 10 µL StickTogether DNA Ligase Buffer 2x (NEB, cat. no. B0535S), 1.5 µL USER Enzyme (NEB ...
-
bioRxiv - Immunology 2019Quote: Plate based scRNA-seq was performed with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs Inc, E6420L). Cells from donor 364b were snap frozen in 10% DMSO in 90% BSA ...
-
bioRxiv - Systems Biology 2022Quote: ... The sample preparation was performed according to the protocol “NEBNext Single Cell/Low Input RNA Library Prep kit for Illumina”(NEB #E6420S/L). Briefly ...
-
bioRxiv - Microbiology 2024Quote: ... 5-10 µg of DNase-treated RNA samples were denatured for 3 min at 70°C and separated on a 15% (w/v) TBE-urea gel (Novex®) with a low range ssRNA ladder (New England Biolabs, USA). The gel was stained with 1x SYBR gold dye (Thermo Fisher Scientific GmbH ...
-
bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 7 ng of RNA per sample using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, E6420). Libraries were pooled and sequenced using a P3-100 kit from Illumina in a NextSeq2000 sequencer following manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 2 ng of RNA per sample using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, E6420). Libraries were pooled and sequenced using a P3-100 kit from Illumina in a NextSeq2000 sequencer following manufacturer’s instructions.
-
bioRxiv - Genomics 2023Quote: First stand cDNA synthesis and cDNA amplification were performed with a NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module (New England Biolabs, MA, USA) and Iso-Seq Express Oligo Kit (Pacific Biosciences (PacBio) ...
-
bioRxiv - Cell Biology 2023Quote: ... and library preparation was performed with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs, catalog # E6420) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Libraries were selected for cDNA target fragments of 200 bp on 2% low-range Ultra agarose followed by PCR amplification using Phusion DNA polymerase (NEB, United States) for 15 PCR cycles ...
-
bioRxiv - Neuroscience 2023Quote: ... sequence samples for Illumina sequencers were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to the manufacturer’s instruction ...
-
bioRxiv - Genetics 2021Quote: ... Injection mix for Cas9 enzyme included Cas9 enzyme (20 μM, New England BioLabs), 2.5 μl of 4x Injection Buffer (0.2% phenol red ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The ladder mix consisted of 500 ng ssRNA ladder (50-1000nt, NEB#N0364S), and 5-10 ng of each 21 & 26-nt RNA oligo loaded per lane ...
-
bioRxiv - Molecular Biology 2021Quote: Chromosomal and plasmidic DNA was digested to nucleosides with Nucleoside Digestion Mix (NEB) and separated on Amicon Ultra-0.5 mL 10K centrifugal filters ...
-
bioRxiv - Molecular Biology 2021Quote: ... 20 μL of 1× PNK mix (2 μL of 10× PNK buffer (NEB), 2 μL ATP (SCP801 ...
-
bioRxiv - Cell Biology 2022Quote: ... 1/10 T7 RNA polymerase mix (HighScribe T7 High Yield RNA synthesis NEB)) at 37°C ...
-
bioRxiv - Genomics 2020Quote: ... The Δtri mutant was created by ligation (using KLD enzyme mix NEB E0554S) of an inverse PCR that omitted the HR-A/B domain (amino acids 342-403 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Beads are then resuspended in Exonuclease I mix (New England Biolabs, Inc., M0293L) and incubate at 37°C for 50min ...
-
bioRxiv - Synthetic Biology 2021Quote: ... cDNA was synthesized immediately afterwards (Lunascript RT Super Mix; New England BioLabs; E3010L) with 800-1000ng total RNA as input (same amount for each condition within experimental repetitions ...
-
bioRxiv - Synthetic Biology 2021Quote: ... restriction enzymes and Gibson assembly mix were obtained from New England Biolabs (NEB) (Ipswich ...
-
bioRxiv - Systems Biology 2020Quote: ... 2 mL of ligation mix (500 μL of T4 Ligase Buffer 10x (NEB), 100 μL of T4 DNA Ligase (400 U/μL ...
-
bioRxiv - Microbiology 2020Quote: The final microinjection mix contained 322 ng/μL spCas9 protein (New England Biolabs) with 40 ng/μL of each sgRNA and 127 ng/μL of the ssDNA repair template ...
-
bioRxiv - Molecular Biology 2021Quote: ... The ladder mix consisted of 500 ng ssRNA ladder (50-1000nt, NEB#N0364S), and 5-10 ng of each 21 & 26-nt RNA oligo loaded per lane ...
-
bioRxiv - Microbiology 2020Quote: ... Nick repair was performed by using the PreCR Repair Mix (New England Biolabs). Lambda exonuclease- and RecJf exonuclease-treated chromatin was eluted from the beads ...
-
bioRxiv - Systems Biology 2022Quote: ... Gibson assembly was performed with NEBuilder Hifi DNA Assembly Mix (New England Biolabs) to circularize the PCR products into entry vectors using the manufacturer’s guidelines ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 100–500 fmol of library was generated with Q5 PCR mix (NEB).
-
bioRxiv - Microbiology 2020Quote: ... and DNA assembly was performed using the NEBuilder HiFi mix (New England Biolabs). All oligos used in this study are listed in Supplementary Table 7 ...