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Citations for New England Biolabs :
4401 - 4450 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... DNA samples were digested to nucleosides using the Nucleoside Digestion Mix (NEB, Ipswich, MA). Global nucleoside content analysis was performed by LC-MS or LC-MS/MS as noted (see conditions below) ...
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bioRxiv - Microbiology 2021Quote: ... The two PCR products were assembled together using the Hifi DNA assembly mix (NEB). The construction was verified by sequencing.
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bioRxiv - Molecular Biology 2022Quote: ... PCR reactions were performed in Q5 HiFi polymerase ready mix (New England BioLabs®) using the following thermocycle ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We performed Golden Gate cloning using Golden Gate Assembly Mix (NEB, Ipswich, MA, #E1601L), following the manufacturer recommended protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Cloning reactions were performed with the NEBuilder HiFi DNA Assembly Mix (New England Biolabs) or by T4 DNA ligase (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2019Quote: ... the samples were added to 2 μL of second-strand synthesis mix (2.5× NEB buffer 2 [New England Biolabs] ...
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bioRxiv - Pathology 2019Quote: ... the pooled samples were end prepped with NEBNext FFPE Repair Mix (New England Biolabs) and NEBNext End repair/dA-tailing Module (New England Biolabs ...
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bioRxiv - Bioengineering 2019Quote: ... the culture media mix was TSB with 1 mg/mL BSA (New England Biolabs) and 50 μM calcein ...
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bioRxiv - Genomics 2021Quote: ... a 30 μl mix with 1.6 μl of T4 DNA ligase (New England Biolabs) was added to each well and placed on a thermocycler (16 °C 60 min followed by 80 °C 30 min for enzyme inactivation) ...
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bioRxiv - Molecular Biology 2019Quote: ... 230 nL DpnI mix was added (1x NEB CutSmart buffer, 0.2 U NEB DpnI) and the plate was incubated at 37°C for 4 hr and 80°C for 20 min ...
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bioRxiv - Molecular Biology 2019Quote: ... 230 nL DpnI mix was added (1x NEB CutSmart buffer, 0.2 U NEB DpnI) and the plate was incubated at 37°C for 4 hr and 80°C for 20 min ...
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bioRxiv - Genomics 2019Quote: ... 4uL of the SMD PCR product was treated with KLD mix (New England Biolabs) and transformed into DH5a E ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Samples 35 and 36 were prepared using the NEBNExt FFPE DNA Repair MIX (NEB). DNA fragment shorter than 20Kb were removed for sample 35 and 36 ...
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bioRxiv - Zoology 2019Quote: ... 4 µL NEB Deoxynucleotide Solution Mix (10 mM each nt) (New England BioLabs, USA), 1 µL Q5® High-Fidelity DNA Polymerase (New England BioLabs ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... 1 μL deoxynucleotide Solution Mix and 0.5 μL Taq DNA Polymerase (New England Biolabs). The reactions were incubated in an Eppendorf Mastercycler Gradient Thermal Cycler for 7m at 95°C ...
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bioRxiv - Microbiology 2021Quote: ... was used to generate single-strand cDNA using random primer mix (NEB, Hitchin, UK). ARTIC V3 PCR amplicons from the single-strand cDNA were generated following the Nanopore Protocol of PCR tiling of SARS-CoV-2 virus (Version ...
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bioRxiv - Genomics 2021Quote: ... DNA was repaired using a NEBNext FFPE DNA Repair Mix (M6630, New England Biolabs) and NEBNext Ultra II End repair/dA-tailing Module (E75460 ...
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bioRxiv - Cancer Biology 2021Quote: ... FFPE DNA samples were treated with the NEBNext FFPE DNA Repair Mix (NEB, M6630L). DNA libraries for Illumina sequencing were prepared with the NEBNext Ultra 2 DNA Library Kit for Illumina (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... and digestion mix was added (375 U DpnII [NEB] in 1x DpnII buffer [NEB]) and cells incubated for 16 h at 37 °C under rotation ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was performed with the Template Switching Reverse Transcriptase enzyme mix (NEB #M0466) according to manufacturer protocols using gene specific primers (vc0512 ...
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bioRxiv - Microbiology 2021Quote: ... 5’ RACE products were generated with Template Switching RT Enzyme Mix (New England Biolabs) using anchored poly(dT)23 and TSO (GCT AAT CAT TGC AAG CAG TGG TAT CAA CGC AGA GTA CAT rGrGrG ...
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bioRxiv - Cell Biology 2021Quote: ... PCR products were circularized using the commercially available KLD Enzyme Mix (New England Biolabs). The resulting plasmids were confirmed by sequencing.
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bioRxiv - Genomics 2021Quote: ... cDNA was prepared from viral RNA using random primer mix (NEB, Massachusetts, United States), and LunaScript® RT SuperMix Kit (Cat# E3010G) ...
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bioRxiv - Cancer Biology 2022Quote: DNA samples were digested to nucleosides using Nucleoside Digestion Mix (New England Biolabs, M0649S). LC-MS/MS analysis was performed by injecting digested DNAs on an Agilent 1290 Infinity II UHPLC equipped with a G7117A diode array detector and a 6495C triple quadrupole mass detector operating in the positive electrospray ionization mode (+ESI) ...
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bioRxiv - Genomics 2022Quote: ... A restriction digestion mix containing 7 µl of 10X NEB CutSmart Buffer (NEB #B7204), 4.5 µl of NlaIII (NEB #R0125) ...
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bioRxiv - Immunology 2022Quote: ... An in-house optimized IVT protocol was used with commercially available rNTP mix (NEB) and commercially available T7 polymerase ...
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bioRxiv - Microbiology 2022Quote: ... The final injection mix contained 300 ng/μL spCas9 protein (M0386, New England Biolabs), 40 ng/μL of each sgRNA (3 targeting exon 5 and 7 of Dicer2 and 4 targeting the kmo gene ...
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bioRxiv - Molecular Biology 2022Quote: ... The linkers that were pre-adenylated using a 5’ DNA adenylation mix (NEB, #E2610L) were ligated to dephosphorylated RNAs using T4 truncated RNA ligase 2 (K227Q ...
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bioRxiv - Evolutionary Biology 2022Quote: ... (1) DNA fragments were repaired with the End-Repair enzyme mix (New England Biolabs). (2 ...
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bioRxiv - Bioengineering 2024Quote: The 5’ RACE protocol using the template-switching reverse transcriptase enzyme mix from NEB was executed following the manufacturer’s guidelines ...
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bioRxiv - Systems Biology 2023Quote: ... The reverse transcription reaction mix (1x RevertAid RT buffer, 250 μM dNTPs (NEB N0447L), 0.2 mg/mL recombinant albumin (NEB B9200S) ...
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bioRxiv - Immunology 2023Quote: ... and 2 µL of home-made Gibson mix (T5 exonuclease (0.2U; New England Biolabs), Phusion polymerase (12.5U ...
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bioRxiv - Molecular Biology 2024Quote: The untreated and treated RNA were digested into nucleosides by Nucleoside Digestion Mix (NEB) in a 50 µl solution according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... gap repaired with HiFi HotStart Uracil+ Ready Mix (Kapa) and Taq DNA ligase (NEB), and treated with a mixture of USER enzyme and T4 polynucleotide kinase (NEB) ...
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bioRxiv - Immunology 2024Quote: ... and PCR amplified and barcoded with NEBNext High-Fidelity PCR Mix (New England Biolabs) and unique dual indexes (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments were assembled using Nebuilder® Hifi DNA Assembly mix (New England Biolabs) following manufacturer’s instructions and incubation at 50°C for 75min.
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bioRxiv - Molecular Biology 2022Quote: ... cDNA samples were PCR-amplified using standard Illumina adaptors and Q5 PCR mix (NEB) with 10-13 cycles (SMInputs ...
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bioRxiv - Molecular Biology 2022Quote: ... The genome was digested with 100nL NLAIII mix (10 nL 1X Cutsmart buffer (NEB), 10 nL NLAIII (Ambion) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 μL of a mix containing 5 U of T4 RNA Ligase 2 (NEB), 1 mM ATP ...
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bioRxiv - Microbiology 2023Quote: ... PCR fragments were gel-purified and incubated with HiFi DNA assembly enzyme mix (NEB) and transfected into E ...
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bioRxiv - Cancer Biology 2023Quote: ... A volume of 40 μl reaction mix containing 5 μl isothermal amplification buffer (NEB), 3 μl 100 mM MgSO4 ...
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bioRxiv - Biophysics 2023Quote: ... 2 pmol were digested to single nucleosides using the Nucleoside Digestion Mix (NEB # M0649), and the ratio of Ψ s versus uridines was determined via tandem quadrupole mass spectrometry (Supplementary Figure S2a).
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bioRxiv - Genomics 2023Quote: ... cDNA (1 µg) was prepared from total RNA using LunaScript cDNA synthesis mix (NEB), diluted 5 times before using it for qRT-PCR ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg of lysates were deglycosylated using Protein Deglycosylation Mix II (NEB Cat# P6044S). In short ...
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bioRxiv - Molecular Biology 2023Quote: ... in a reaction mix containing 1× T4 RNA Ligase Buffer (New England Biolabs, M0437M), 1U SUPERase•In RNase inhibitor (Invitrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1ng of RNA was added to Luna Universal One-Step qRT-PCR mix (NEB) containing 4μmol of each primer on ice ...
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bioRxiv - Microbiology 2023Quote: ... The linearized vector was assembled using the HiFi DNA assembly mix (New England Biolabs) with a Pbiofab-mcherry fragment ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified plasmid was gel purified and then circularized with KLD Enzyme Mix (NEB, M0554).
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bioRxiv - Developmental Biology 2024Quote: ... were assembled using NEBulider HiFi DNA Assembly Mix (New England Biolabs, catalog no. E2621L). These constructs were then cloned into PCR4-Shh:LacZ-H11 plasmids (Addgene plasmids ...
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bioRxiv - Cancer Biology 2024Quote: ... by directly adding 5U of calf intestine phosphatase to the mix (New England Biolabs) in 100 mM Tris-HCl ...