Labshake search
Citations for New England Biolabs :
4351 - 4400 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Thawed RNA specimens were converted to cDNA using the Luna RT mix (NEB). Arctic multiplex PCR primer pools (IDT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... followed by DpnI digestion and blunt end ligation using KLD enzyme mix (NEB). Sabaeus TRIM5α (XM_008019878.2) ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR reaction mix was created with NEB Phusion GC Buffer (NEB, B0519S), Phusion DNA Polymerase (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Gibson assemblies were transformed the assembly mix into DH5α competent cells (NEB #C2987H). Plasmid backbones were digested with restriction enzymes and purified using Qiagen QIAquick gel extraction kit (#28706) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µl of tailing mix (0.05 µl Terminal Transferase (NEB, cat. no. M0315), 0.1 µl 10x Cutsmart buffer ...
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bioRxiv - Genomics 2020Quote: ... Quantification of libraries was done by qPCR using the NEBNext library quant kit for Illumina (NEB, E7630) and library sizes were analyzed using the Agilent bioanalyzer 2100 HS DNA kit ...
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bioRxiv - Microbiology 2019Quote: ... we performed q-RT PCR using the Luna Universal One-Step RT-qPCR Kit from NEB (E3005), using the Applied BiosystemsΤΜ 7500 Real-Time PCR system ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and IFIH1 were quantified using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs #E3005L) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized for qPCR quantification using the LunaScript RT SuperMix Kit (New England Biolabs; Massachusetts, USA). qPCR reactions were composed of 7.5 μL Power SYBR Green PCR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the purified amplicons was quantified by qPCR using the NEBNext Illumina Quant kit (NEB). The forward and reverse reads were demultiplexed and fused barcodes were quantified through alignment against custom barcode and primer sequences using Bowtie2 (V2.3.4.1 ...
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bioRxiv - Molecular Biology 2023Quote: ... All qRT-PCR reactions were performed with the Luna Universal One-Step RT-qPCR kit (E3005 NEB) according to manufacturer protocol with a few modifications ...
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bioRxiv - Microbiology 2023Quote: We performed RT-qPCR on select genes using the Luna Universal One-Step Kit (New England Biolabs) and QuantStudio 5 (Thermo) ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng of total RNA was used with Luna Universal One-Step RT-qPCR (NEB, Ipswich, MA) to detect StayGold mRNA expression ...
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bioRxiv - Microbiology 2024Quote: ... Barcodes (Native Barcoding Kit 96 (SQK-NBD109.96)) were ligated using Blunt/TA Ligase Master Mil (NEB). Barcoded libraries were purified using KAPA Pure Beads (Roche ...
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bioRxiv - Genomics 2021Quote: ... The isolated total RNA was converted into cDNA with NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England BioLabs, Ipswich, MA, USA). A sequence library for isoform sequencing (Iso-Seq ...
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bioRxiv - Immunology 2022Quote: ... Libraries were size selected for cDNA target fragments of 300 bp on 2% Low Range Ultra Agarose followed by PCR amplification using Phusion DNA polymerase (New England Biolabs, Ipswich, MA, USA). The expression of each transcript was calculated according to the transcripts per million reads method ...
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared in the Duke Center for Genomic and Computational Biology’s Sequencing and Genomic Technologies Core Facility using the NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module (NEB, catalog no. E6421) and SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences ...
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bioRxiv - Neuroscience 2022Quote: RNA library preparations for transcriptome analysis were performed using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs, #E6420) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... RNA Library preparations for Synpo-related transcriptome analysis were performed using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs, #E6420) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The library preparation of the total RNA was performed with the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB #E6420S/L). Single read sequencing with a read length of 72 bp was performed on NextSeq ® 2000 System using the corresponding NextSeq2000 P3 Reagent Kit (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were sorted into 750 uL TRIzolTM LS and libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, version 3.0, #E6420L) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
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bioRxiv - Genomics 2023Quote: The PacBio Iso-Seq full-length sequencing library was prepared using 300 ng of total RNA using NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module (NEB, catalog no. E6421) and SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences ...
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bioRxiv - Neuroscience 2023Quote: RNA Library preparations for transcriptome analysis were performed using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs, #E6420) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng RNA were used for analysis with the LUNA one-step RT-qPCR kit (LUNA E3005L Biolabs). The relative expression levels of the mRNA of interest were determined by real-time PCR using Quantifast SYBR Green Mix (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... and the quantity of the library was determined by qPCR using NEBNext Library Quant Kit for Illumina (NEB) according to manufacture’s instruction ...
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bioRxiv - Bioengineering 2020Quote: ... The RT-PCR detection was conducted by using the Luna Universal One-Step RT-qPCR kit from NEB and following its protocol ...
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bioRxiv - Microbiology 2022Quote: ... Detection of selected targets was performed with Luna® Universal One-Step RT-qPCR (New England BioLabs Inc.) according to manufacturers protocol using specific primers ...
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bioRxiv - Molecular Biology 2023Quote: ... Extracted RNA from light and heavy fractions were subjected to qRT-PCR using Luna RT-qPCR kit (NEB).
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bioRxiv - Developmental Biology 2024Quote: ... RNA was then directly used in the Luna® Universal One-Step RT-qPCR Kit (New England BioLabs). Primers listed in supplemental table 10.
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bioRxiv - Microbiology 2023Quote: ... cDNA synthesis and qRT-PCR were performed simultaneously using the Luna Universal One-Step RT-qPCR kit (NEB) with the Quantstudio 7 Flex (Life Technologies ...
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bioRxiv - Genomics 2023Quote: ... RNA expression levels were quantified using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs #E3005L) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: PCR was carried out using NEB Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs, E3006) or Taqman Fast Universal PCR master mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... Library fragment size and concentrations were quality verified by qPCR using the NEBNext Library Quant Kit (NEB, E7630) and loading 1:1 ...
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bioRxiv - Cell Biology 2020Quote: ... and digestion mix was added (375 U DpnII [NEB] in 1x DpnII buffer [NEB]) and cells incubated for 16 h at 37 °C under rotation ...
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bioRxiv - Microbiology 2020Quote: ... The gel-purified PCR fragments were incubated with HiFi DNA assembly enzyme mix (NEB) and transformed into E ...
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bioRxiv - Genomics 2019Quote: ... then 20 µL of the end-repair mix [3.5X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix ...
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bioRxiv - Genomics 2020Quote: ... Second strand synthesis was carried out (Second Strand Synthesis Enzyme mix; New England Biolabs) and the dsDNA was used to generate a library for sequencing on an Illumina MiSeq ...
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bioRxiv - Genomics 2021Quote: ... using the NEBNext FFPE DNA Repair Mix (New England BioLabs Inc, NEB; Ipswich MA). Purification of the repaired DNA extract was performed using the QIAGEN MinElute PCR Purification Kit ...
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bioRxiv - Genomics 2021Quote: ... using the NEBNext FFPE DNA Repair Mix (New England BioLabs Inc, NEB; Ipswich MA). Purification of the repaired DNA extract was performed using the QIAGEN MinElute PCR Purification Kit ...
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bioRxiv - Immunology 2021Quote: ... Samples were added to second-strand synthesis mix containing 2× NEB buffer 2 (NEB), 625 nM dNTP Mixture (NEB) ...
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bioRxiv - Genomics 2021Quote: ... end-repairing and dA-tailing was achieved using the NEBNext FFPE Repair Mix (NEB) and the Ultra II end-repair module (NEB) ...
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Machine learning-guided acyl-ACP reductase engineering for improved in vivo fatty alcohol productionbioRxiv - Synthetic Biology 2021Quote: ... Golden Gate reactions were carried out either using commercial Golden-Gate assembly mix (NEB), or an in-house mixture of the components from NEB (T4 DNA ligase buffer ...
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bioRxiv - Systems Biology 2022Quote: ... 20 μl of ligation mix (30 U/μl T4 DNA ligase (cat. # NEB M0202L), and 1.58 X ligase buffer ...
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bioRxiv - Genomics 2022Quote: ... we first repaired DNA molecules with the NEBNext FFPE DNA Repair Mix (NEB M6630) and NEBNext Ultra II end repair/dA-tailing Module (NEB E7546) ...
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bioRxiv - Molecular Biology 2022Quote: ... 22.5 uL Rnase-free water and 50 µL Blunt/TA Ligase Mix (NEB, M0367S) and incubated in room temperature for 10 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... 22.5 µL Rnase-free water and 50 µL Blunt/TA Ligase Mix (NEB, M0367S) and incubated in room temperature for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... and PCR amplified and barcoded with NEBNext High-Fidelity PCR Mix (New England Biolabs) and unique dual indexes (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... was used to generate single-strand cDNA using random primer mix (NEB, Hitchin, UK). ARTIC V3 PCR amplicons from the single-strand cDNA were generated following the Nanopore Protocol of PCR tiling of SARS-CoV-2 virus (Version ...
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bioRxiv - Developmental Biology 2021Quote: ... custom NTP mix was prepared with 3’-O-Me-m7G cap analogue (60mM, NEB), GTP (75mM ...
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bioRxiv - Cell Biology 2021Quote: ... the RNP injection mix [5 µM EnGen® Spy Cas9 NLS (NEB Cat# M0646T) mixed with 2.5 µM of each gRNA and Cas9 buffer] was freshly prepared ...