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Citations for New England Biolabs :
4001 - 4050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Final nuclear lysates were resuspended using 100 µl of resuspension solution (1x PBS, 2% BSA, 0.2U/µl RNase inhibitor; New England Biolabs, cat# M0314S). Hoechst staining was performed to assess the quality of isolated nuclei based on their morphology ...
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bioRxiv - Neuroscience 2024Quote: ... All lentiviral constructs were generated through the Gibson assembly method (NEB) with the corresponding cDNAs and with a human synapsin-1 promoter-driven lentiviral shuttle vector (f(syn) ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated with T4 ligase (NEB, 1 U/ml T4 DNA ligase ...
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bioRxiv - Neuroscience 2024Quote: After washed with 0.1 3 SSC buffer (Thermo, AM9770) supplemented with 0.05 U/ml RNase inhibitor (NEB, M0314L), tissue sections placed on the chip were permeabilized using 0.1% pepsin (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... These two oligos were ligated with splint oligo1 at 37°C for 2 hours using T4 ligase (NEB; 1U/ml T4 DNA ligase and 13 T4 DNA ligation buffer) ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries for sequencing were constructed with the NEB Next Ultra RNA Library Prep Kit (NEB) for Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... Taq polymerase (New England Biolabs, Inc.) was used for standard PCR reactions in a Bio-Rad T100 thermal cycler using a 57°C annealing temperature.
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bioRxiv - Plant Biology 2024Quote: ... we amplified the mTURQUOISE2 gene from vector C440010 using primers FHOx90/FHOx91 and Q5 DNA Polymerase (New England Biolabs) and combined the PCR product with modules pICH47742 ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra-II RNA kit (New England Biolabs) according to a previously described protocol27 ...
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bioRxiv - Microbiology 2024Quote: ... the NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (New England BioLabs) and UltraPureTM BSA (50 mg/mL ...
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bioRxiv - Microbiology 2024Quote: ... We used the Q5 High-Fidelity DNA polymerase (NEB) to PCR amplify the ermB gene that provides resistance to erythromycin (using pMTL83251 as the template ...
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bioRxiv - Microbiology 2024Quote: ... DNA was subsequently purified with a Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... or NlaIII (New England Biolabs, Ipswich, MA, USA) restriction enzyme ...
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bioRxiv - Plant Biology 2024Quote: ... 2µg of recombinant protein was mixed with 400U lambda protein phosphatase (NEB, #P0753S) at 30°C for 30 min before separated by an SDS-PAGE ...
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bioRxiv - Plant Biology 2024Quote: ... of genomic DNA fragments were repaired and end-prepped with the NEBNext FFPE DNA Repair Mix and the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs). Barcodes provided by ONT were then ligated using the Blunt/TA Ligase Master Mix (NEB) ...
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GIBBERELLIN SIGNALING THROUGH RGA SUPPRESSES GCN5 EFFECT ON STAMEN ELONGATION OF ARABIDOPSIS FLOWERSbioRxiv - Plant Biology 2024Quote: ... or the Luna® Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA) at the ABI StepOnePlus™ system (Applied Biosystems ...
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Asymptomatic neonatal herpes simplex virus infection in mice leads to long-term cognitive impairmentbioRxiv - Microbiology 2024Quote: ... qPCR reactions were performed using the following components to a final reaction volume of 20 μl: 1X Luna Universal qPCR Master Mix (New England Biolabs), forward and reverse primers at a final concentration of 0.25 μM ...
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bioRxiv - Cell Biology 2024Quote: ... Primer amplicons were generated through PCR using Q5 Hot Start High-Fidelity 2X Master Mix (NEB #M0494). G-protein-coupled receptors (Ste2 ...
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bioRxiv - Cell Biology 2024Quote: ... PCR verification was performed using OneTaq 2X Master Mix with Standard Buffer (NEB #M0482) or LongAmp Hot Start Taq 2X Master Mix (NEB #M0533).
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bioRxiv - Cell Biology 2024Quote: ... Every 2 PCR reactions were pooled and purified in 100ul elution buffer using Monarch PCR & DNA Cleanup Kit (7:1 binding buffer, NEB#T1030L). 5ul/reaction Dynabeads M270 Streptavidin (Thermofisher#65305 ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were purified using Monarch DNA Gel Extraction Kit (New England Biolabs). Both the vector and the complete insert were digested by BsmBI (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription (RT) of RNA was performed using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs), in accordance with the manufacturer’s manual ...
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bioRxiv - Cell Biology 2024Quote: ... and ligated using T4 DNA ligase (New England Biolabs). The ligated product was transformed into MegaX DH10B T1R cells (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Both the vector and the complete insert were digested by BsmBI (New England Biolabs) and ligated using T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... The inserts and the backbone were mixed in a 3:1 ratio and incubated with the 2x NEBuilder HiFi DNA assembly enzyme mix (New England Biolabs) for 1h at 50°C ...
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bioRxiv - Cell Biology 2024Quote: ... and clean-up performed using Monarch® RNA Cleanup Kit (Biolabs, New England, T2030L).
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bioRxiv - Cell Biology 2024Quote: ... and complexed with Cas9 (1.5 µL 1 µM, NEB). CRISPR/Cas9 complexes and 100 pmol annealed repair template were electroporated (Neon transfection system ...
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bioRxiv - Cell Biology 2024Quote: ... An RNA-seq library was prepared using an NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) after ribosomal RNA (rRNA ...
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bioRxiv - Cell Biology 2024Quote: ... Fragments were purified before mixing with HiFi Assembly Mastermix (NEB) for 30’ at 50°C ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting assembly mixture was transformed into chemically competent Escherichia coli (C2987H) through a High Efficiency Transformation (NEB). Colonies were isolated via growth on selective media (LB carbenicillin+) ...
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bioRxiv - Cell Biology 2024Quote: ... or LongAmp Hot Start Taq 2X Master Mix (NEB #M0533).
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bioRxiv - Cell Biology 2024Quote: ... ABHD17A mutant plasmids (plasmids 5-21 in Supplementary Table 1) were made with NEBuilder HiFi DNA Assembly (New England Biolabs), using primers (Supplementary Table 2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells undergoing mitosis were tracked from nuclear envelope breakdown (NEB) to anaphase onset (AO) ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was then digested with DpnI (NEB #R0176S).
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bioRxiv - Cell Biology 2024Quote: ... we replaced the EGFP from the previously generated EGFP-Myo10MD-FRB (above) with GCN4 repeats from pHRdSV40-K560-24xGCN4_v4 (addgene #72229) via Gibson assembly (NEB #E2621S) using the following primer sets ...
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bioRxiv - Cell Biology 2024Quote: ... 100 pmol forward oligo and 100 pmol reverse oligo were resuspended in 25 μL 1x NEBuffer 2 (NEB, B7002S). The solution was incubated in a 95 °C water bath for 4 min then slowly cooled (∼0.5 °C/min ...
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bioRxiv - Cell Biology 2024Quote: ... RNA-DNA hybrids were removed by incubating cells with 0.0S U/µL RNAse H (M0297S, NEB) for 20 min at 37 °C in RNAse H buffer (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by NEB NEBNext Ultra II (NEB #E7760 ...
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bioRxiv - Cell Biology 2024Quote: ... and purified using an NEB clean and concentrate kit (NEB #T2030).
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bioRxiv - Cell Biology 2024Quote: ... mRNA was enriched with magnetic oligo (dT) beads (New England Biolabs, cat#S1419S). The cDNA library was constructed with mean inserts of 200bp with non-stranded library preparation using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... The cDNA library was constructed with mean inserts of 200bp with non-stranded library preparation using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, cat#E7530L). Sequencing was performed by a paired-end 125 cycles rapid run on the Illumina HiSeq2500 ...
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bioRxiv - Cell Biology 2024Quote: ... the newly produced CENP-A-SNAP pool was labelled by exposing the mitotic cells to media containing 3 µM SNAP-Cell 647 (NEB) and 5 µM STLC (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... existing CENP-A-SNAP proteins were labelled for 30 min using 10 µM SNAP-Cell Block (NEB) and cells were exposed to media containing 5 µM S-trityl-L-cysteine (STLC - Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... in the presence of RNase inhibitor (NEB, M3014) at 37°C for 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... Mutagenic PCR products were then digested with 10 U DpnI (New England Biolabs) at 37°C for 30 min to eliminate template DNA ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using the Monarch Total RNA Miniprep Kit (New England BioLabs #T2010S) and submitted to Azenta Life Sciences for library preparation and Illumina sequencing.
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bioRxiv - Cell Biology 2024Quote: ... RNA was then purified using Monarch Total RNA miniprep Kit (New England Biolabs, T2010S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Gibson Assembly of the three generated fragments was performed using NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621). The resulting assembly mixture was transformed into chemically competent Escherichia coli (C2987H ...
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bioRxiv - Cell Biology 2024Quote: ... The mutated product was transformed into chemically competent Escherichia coli (C2987H) through a High Efficiency Transformation (NEB). Colonies were isolated via growth on selective media (LB carbenicillin+) ...
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bioRxiv - Cell Biology 2024Quote: ... and plasmids were purified using a Monarch Plasmid Miniprep Kit (NEB #T1010). Successful mutagenesis was verified through DNA sequencing (Plasmidsaurus).