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Citations for New England Biolabs :
3951 - 4000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Polyadenylated mRNA (polyA mRNA) was selected with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs [NEB], cat# E7490L) and polyA mRNA sequencing libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Genomics 2024Quote: ... Ribosomal RNA was depleted from total input RNA using the NEBNext rRNA Depletion Kit (NEB cat. #E6310). First and second strand synthesis ...
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bioRxiv - Genomics 2024Quote: ... 120 μg of total liver RNA was isolated from 60-100 mg liver tissue from each HDI-STARR-seq-treated mouse by TRIzol guanidinium thiocyanate-phenol-chloroform extraction then purified by polyA selection using oligo(dT) beads (New England Biolabs, cat. #E7490L). The purified RNA was digested with Turbo DNase-I (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... dNTPs and hot start Taq polymerase (New England Biolabs Inc, Ipswich, MA). A construct specific primer (Forward ...
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bioRxiv - Plant Biology 2024Quote: ... then pooled and ONT adapters were added using the NEBNext Quick Ligation Module (NEB). After purification with AMPure XP beads (Beckmann Coulter) ...
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bioRxiv - Plant Biology 2024Quote: ... Genes of interest were cloned to Gateway™ pENTR™ 4 Dual Selection Vector (A10465) by NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621L) and cloned to a pUC19 expression vector powered by the maize ubiquitin promoter (ZmUBI ...
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bioRxiv - Plant Biology 2024Quote: ... Barcodes provided by ONT were then ligated using the Blunt/TA Ligase Master Mix (NEB). Barcoded fragments were purified with AMPure XP beads (Beckmann Coulter) ...
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bioRxiv - Microbiology 2024Quote: ... The Primer-free LunaScript® RT Master Mix Kit (New England Biolabs) was used for first strand cDNA synthesis using a gene-specific primer targeting the mCherry gene in the genomic vRNA (primer sequence 5’-CGCTTCAAGGTGCACATGG-3’) ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were then prepared from 20ng of input DNA using the NEBNext Ultra-II DNA kit (New England Biolabs) following manufacturer’s instructions22 ...
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bioRxiv - Neuroscience 2024Quote: ... was used to replace the CMV promoter in pAAV.U6.shRLuc.CMV.ZsGreen.SV40 (Penn Core Vector) plasmid by excising out the CMV promoter using the EcoRI (NEB #R3101) and NHE-I (NEB #R3131 ...
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bioRxiv - Microbiology 2024Quote: ... probe-based qPCR was carried out using Luna Universal Probe qPCR Master Mix (New England Biolabs) and the QuantStudio3 Real-Time PCR System (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... and Gas5 shRNAs (shRNA # 1= G5C1 and shRNA # 2= G5C2) were cloned by replacing the existing Luciferase shRNA (shRLuc) under a U6 promoter using BamHI (NEB #R0136L) and EcoRI restriction sites (Table 3) ...
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bioRxiv - Biochemistry 2024Quote: ... and ligated to pre-adenylated linkers (NI-810 to NI-815) containing 5 nt sample barcodes unique for each sample using truncated T4 RNA ligase 2 (K227Q) (NEB; M0351L). Ligated fragments were separated from free linkers on a 15% polyacrylamide TBE-Urea gel and then pooled and purified for reverse transcription using RT primer NI-802 and ProtoScript II Reverse Transcriptase (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... The RNA-Seq library-preparation protocol was based on the NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, #E7530L). Insert size was assessed using the Agilent Bioanalyzer 2100 system and qualified insert size was accurate quantification using StepOnePlus™ Real-Time PCR System (Library valid concentration>10nM ...
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bioRxiv - Plant Biology 2024Quote: ... was cloned in front of the second codon of GFP by Golden Gate cloning (Engler et al., 2008) using BbsI-HF (NEB). Enhancers ...
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bioRxiv - Plant Biology 2024Quote: ... and rbcS-E9 enhancers and the synthetic enhancers syn1–11 were cloned upstream of the 35S minimal promoter in pDL by Golden Gate cloning using BsaI-HFv2 (NEB). The synthetic enhancers were ordered as synthesized DNA fragments.
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bioRxiv - Plant Biology 2024Quote: ... and enhancer fragment combinations were cloned upstream of the 35S minimal promoter by Golden Gate cloning using BsaI-HFv2 (NEB). The resulting libraries were bottlenecked to yield 10–20 barcodes per enhancer.
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bioRxiv - Biochemistry 2024Quote: ... validation mutants pAG416-GPD-PjDHFR-M33P and pAG416-GPD-PjDHFR-F199P) or pAG416-GPD-ScDHFR were digested using ClaI enzyme in rCutSmart® buffer (New England Biolabs, catalog #R0197) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and T4 RNA Ligase 1 (NEB, #M0204S), respectively ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were digested using 0.2 μL of DpnI restriction enzyme (New England Biolabs, catalog #R0176L) (37 °C for 2 h ...
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bioRxiv - Biochemistry 2024Quote: ... the library was amplified using Q5 High-Fidelity DNA Polymerases with GC enhancer (NEB, #M0491S) and subjected to size selection using a 6% polyacrylamide gel ...
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bioRxiv - Biochemistry 2024Quote: ... 50 ng of GLORI-treated RNA sample was repaired using T4 Polynucleotide Kinase (NEB, #M0201S). Pre-adenylated 3’-adapter and 5’-adapter were sequentially ligated to the RNA using T4 RNA Ligase 2 ...
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bioRxiv - Plant Biology 2024Quote: ... Sonicated samples were used for purification by affinity chromatography using amylose resin (New England Biolabs) for MBP-NAC53/78 or glutathione sepharose 4B (cytiva ...
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bioRxiv - Synthetic Biology 2024Quote: ... the barcode and sensor regions of the biosensor libraries were amplified using Q5 PCR (New England Biolabs) directly off 5 μL of glycerol stock using primers p117 and p118 (Table 2) ...
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bioRxiv - Synthetic Biology 2024Quote: ... TCSs were amplified by PCR using Q5 High-Fidelity DNA polymerase (New England Biolabs), and amplified PCR fragments were purified by column purification using the Monarch PCR Cleanup Kit (New England Biolabs ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were purified using a Monarch PCR Cleanup Kit (New England Biolabs) and prepared for nanopore long read sequencing using the Ligation Sequencing Kit (Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2024Quote: ... 2U/μL RNAse inhibitor murine (New England Biolabs), 0.25 μM 6UFAM (Table S3) ...
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bioRxiv - Neuroscience 2024Quote: ... with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (NEB). All libraries were sequenced 150bp paired end using an Illumina Novaseq 6000 with 15 million reads on average per sample.
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bioRxiv - Synthetic Biology 2024Quote: ... and amplified PCR fragments were purified by column purification using the Monarch PCR Cleanup Kit (New England Biolabs) following the manufacturer’s protocols.
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bioRxiv - Plant Biology 2024Quote: ... cDNA synthesis was performed using LunaScript® RT SuperMix Kit (NEB) following provider recommendation ...
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bioRxiv - Plant Biology 2024Quote: ... 5 and 7dpi using a total RNA extraction kit (NEB) and following the kit instructions ...
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bioRxiv - Physiology 2024Quote: ... the pLV6-Bmal-luc vector was digested with BamHI (NEB, R3136S) then treated with quick CIP (NEB ...
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bioRxiv - Physiology 2024Quote: ... This cDNA then was re-linearized by Ape1 (NEB), size selected by TBE gel electrophoresis and the products of the desired size were excised (∼320–350 bp ...
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bioRxiv - Physiology 2024Quote: ... then treated with quick CIP (NEB, M0525S) to avoid self-ligation ...
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bioRxiv - Neuroscience 2024Quote: ... and NotI-HF (NEB #R3189) to excise out ZsGreen ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA (30 μl) was poly(A) tailed using 20 units terminal transferase (TdT, NEB, #M0315) and 0.2 mM dATP for 15 min at 37°C in TdT buffer (20 mM Tris-HCl pH 7.9 ...
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bioRxiv - Developmental Biology 2024Quote: ... mRNA was synthesized from linearized plasmid using HiScribe T7 high yield RNA synthesis kit (NEB), then capped with m7G (5’ ...
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bioRxiv - Cell Biology 2024Quote: ... The insertion was done using the Q5 Site-Directed Mutagenesis Kit (NEB) and design of respective primers using the NEBaseChanger online tool (http://nebasechanger.neb.com/).
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bioRxiv - Evolutionary Biology 2024Quote: ... Homology arms of the Or49 gene flanking 1 kb upstream and downstream of the desired insertion site (Supplementary Fig. 6a) were amplified with the Q5 High Fidelity DNA polymerase (New England Biolabs, Ipswich, MA), using primer pairs Left_Arm_Or49_FWD and Left_Arm_Or49_REV as well as Right_Arm_Or49_FWD and Right_Arm_Or49_REV ...
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bioRxiv - Cancer Biology 2024Quote: ... 62.5 units per 1 million cells MNase (NEB; M0247S) was added to resuspended pellets ...
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bioRxiv - Physiology 2024Quote: ... the pLV6 backbone and the Per2:luciferase fragment were ligated with T4 DNA ligase (NEB, M0202S) according to the manufacture’s manual ...
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bioRxiv - Cell Biology 2024Quote: ... by linearizing the vector with 15 base pairs (bp) overlapped primers using Phusion high-fidelity DNA polymerase (New England Biolabs, cat. no. MO531L). PCR products were digested using Dpn I (Takara ...
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bioRxiv - Biochemistry 2024Quote: ... and assembled into a digested (HindIII, rCutSmart®, New England Biolabs, catalog #R0104) and purified pAG416-GPD-ccdB using in-house Gibson assembly ...
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bioRxiv - Biochemistry 2024Quote: ... truncated KQ (NEB, #M0373S) and T4 RNA Ligase 1 (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... pAG416-GPD-ccdB was digested using XbaI and HindIII-HF in CutSmart® buffer (New England Biolabs, catalog #R0145 and #R0104), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... A 673 bp digoxigenin-labeled DNA handle was amplified in Q5U PCR master mix (NEB, Cat# M0597L) with a forward primer containing a deoxyuridine and a reverse primer with a 5′ digoxigenin tag ...
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bioRxiv - Neuroscience 2024Quote: ... was used to construct sequencing libraries using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, NEB). Libraries were multiplexed using NEBNext Multiplex Oligos for Illumina (dual index primers ...
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bioRxiv - Neuroscience 2024Quote: ... was used to construct sequencing libraries using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, NEB). Libraries were multiplexed using NEBNext Multiplex Oligos for Illumina (dual index primers ...
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bioRxiv - Neuroscience 2024Quote: ... 5 ng of ChIP and Input DNA was used to generate ChIP-seq libraries using the NEBNext Ultra DNA library prep kit for Illumina (New England Biolabs, NEB).
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bioRxiv - Neuroscience 2024Quote: ... or NotI (NEB Cat # 189S) restriction endonuclease (depending on insert orientation) ...