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Citations for New England Biolabs :
3851 - 3900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... 75mer oligonucleotides comprising the amiRNAs and SisRNAs sequences were cloned into the BsaI-sites by Golden Gate strategy using BsaI-HF®v2 (NEB, R3733) and T4 DNA Ligase (Thermo Fischer Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid assembly was performed using NEBuilder HiFi DNA Assembly Master Mix (NEB), followed by transformation into NEB 5-alpha competent E ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA amplification for plasmid construction was performed using Q5 Hot Start High-Fidelity 2x Master Mix (NEB), and colony PCR was performed using Phire Green Hot Start II PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2024Quote: ... These PCR products were used for Gibson assembly with the barcoded promoters (#E2611S; NEB). Gibson assembly reactions were purified with magnetic beads and 2 µl of the reaction were electroporated into 20 µl of electrocompetent e ...
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bioRxiv - Systems Biology 2024Quote: ... and barcode-insert fragments were linearized again with I-SceI (#R0694S; NEB). Barcode-insert fragments were then amplified by PCR with Illumina adapters and sequenced on an Illumina NextSeq 550 platform using 150 bp paired-end sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were stained with ush Stellaris and lacZ Stellaris probes (Frampton et al., 2022) and nuclei were stained with DAPI (1:1000, NEB 4083). Samples were mounted in ProLong Diamond antifade mountant (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: ... system following the manufacturer’s instruction with one additional DNAse (NEB) treatment step ...
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bioRxiv - Cancer Biology 2024Quote: ... range 9.0-9.5] were converted into sequencing libraries with the “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (#E7760, New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Exon 3 of Smad4 was amplified by PCR using the Q5 High Fidelity DNA polymerase (M0491S; NEB). Smad4 primers used were FWD ...
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bioRxiv - Immunology 2024Quote: ... The T7 sgRNA PCR product (500 ng) was used as a template for in vitro transcription with the T7 High Yield RNA synthesis kit (NEB, cat # E2040S). The reaction was incubated at 37°C for 4 hours ...
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bioRxiv - Plant Biology 2024Quote: ... The RFP insert was digested from the pBeaconRFP_GR vector (https://gatewayvectors.vib.be/index.php/ ID: 3_20, Bargmann & Birnbaum, 2009) using the restriction enzyme NdeI (NEB, R0111S) and further ligated into the NdeI site of the pUC18-AtMIR390a-B/c vector ...
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bioRxiv - Microbiology 2024Quote: ... a 20uL 16S-V4 PCR reaction was set up (1ng extracted gDNA; 1uL forward barcoded P5 primer; 1uL reverse barcoded P7 primer; 10uL NEBNext® Ultra™ II Q5® Master Mix [NEB M0544X] ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated with micrococcal nuclease (M0247S, Biolabs) at 37° for 10 min ...
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bioRxiv - Genomics 2024Quote: ... Individual libraries for immunoprecipitated DNA and 10% input were dual indexed (NEBNext Multiplex Oligos, unique dual indices, New England Biolabs), PCR amplified and then pooled into equimolar amounts and further quantified using the NEBNext Library Quant Kit (New England Biolabs) ...
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bioRxiv - Genetics 2024Quote: ... and 0.5–50 ng of DNA/ChIP was used for library preparation using the NEBNext Ultra II DNA Library Kit for Illumina (NEB E7645) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 and 2 ...
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bioRxiv - Microbiology 2024Quote: ... 0.025 U Taq Pol (New England Biolabs), 1x Thermo Pol Buffer (New England Biolabs) ...
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bioRxiv - Physiology 2024Quote: ... Five hundred micrograms of total RNA was used to prepare strand-specific libraries by the NEBNext Ultra II Directional RNA Library Preparation kit (NEB) and sequenced (75 nucleotides ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg of RNA was used as input for library preparation using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (NEB E7560S), according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 ng of total RNA was used for RT-qPCR using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, cat# E3005L) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The culture supernatants (150 μL) were treated with DNase I (NEB, Ipswich, MA, U.S.A.) to obtain enveloped and encapsidated viral genomes ...
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bioRxiv - Microbiology 2024Quote: ... phi47 orf65 (tifA) and orf65-L32F were PCR amplified using Q5 master mix (New England Biolabs) and cloned into pLZ12A ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 1-2 µg phi47 DNA was treated with Nucleoside Digestion Mix (New England Biolabs) following the manufacturer’s protocol at 37°C for >2h ...
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bioRxiv - Microbiology 2024Quote: ... Hydrogel chitin particles (New England Biolabs, #E8036L) were washed in sterile artificial seawater (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.25 uL of mRNA Second Strand Synthesis enzyme (New England Biolabs, E6111L). The plates were incubated at 16°C for 3 hours ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 uL of 5 x mRNA Second Strand Synthesis buffer (New England Biolabs, E6111L), and 0.25 uL of mRNA Second Strand Synthesis enzyme (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: The PURExpress ΔRibosome In Vitro Protein Synthesis Kit (New England Biolabs) was used for in vitro translation assays ...
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bioRxiv - Microbiology 2024Quote: ... using SmaI (NEB) and T4 DNA ligase (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... and 500 ng of DNA was digested with 5U NcoI and BamHI restriction enzymes (New England Biolabs, Ipswich, MA) overnight at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids were assembled using the NEBuilder HiFi DNA Assembly kit (New England BioLabs). The different truncated or point mutants derived from these plasmids were generated by PCR amplification using primers (Supplementary Table 6 ...
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bioRxiv - Microbiology 2024Quote: ... The radioactive probe was prepared from 40 pmoles of primers described in Supplementary Table 7 and labeled at the 5’ end with 10 units of T4 polynucleotide kinase (New England Biolabs) and [γ-32P]-ATP (150 µCi) ...
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bioRxiv - Microbiology 2024Quote: ... 1x Thermo Pol Buffer (New England Biolabs), 5 nM DNA matrix ...
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bioRxiv - Immunology 2024Quote: ... Illumina compatible cDNA libraries generated for polyA selected mRNA using NEB next ultra RNA library preparation kit Cat#E7530L (New England Biolabs Inc, MA). The cDNA libraries were purified using AmpureXP beads ...
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bioRxiv - Genetics 2024Quote: ... Mutant hEZH2 ORFs were generated by Q5 Site-Directed Mutagenesis (NEB E0554S) as per the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 and 2; NEB E7335 and E7500). Library DNA was quantified using the Qubit ...
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bioRxiv - Genomics 2024Quote: ... PCR amplified and then pooled into equimolar amounts and further quantified using the NEBNext Library Quant Kit (New England Biolabs). The pooled library was subjected to 50 bp paired-end sequencing on the Illumina NextSeq 2000 platform.
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Evolutionary Adaptation of an HP1-protein Chromodomain Integrates Chromatin and DNA Sequence SignalsbioRxiv - Genetics 2024Quote: ... Barcoded libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to manufacturer’s instructions and sequenced on a NovaSeqSP instrument (Illumina).
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bioRxiv - Neuroscience 2024Quote: ... or 5 µL of O-Glycosidase (P0733L, NEB) where indicated prior to denaturation/loading on SDS-PAGE gels according to manufacturer protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 µL Purple Dye (New England Biolabs, B7025S), and 4 uL ddH20 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Standard Taq (New England Biolabs #M0273) or Phusion (New England Biolabs #M0530 ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... or Phusion High-Fidelity PCR Master Mix (NEB, M0531S). After agarose gel purification using columns (QIAGEN) ...
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bioRxiv - Cancer Biology 2024Quote: ... Second strand synthesis was performed using Klenow Exo- (NEB, Cat: M0212L) in Maxima RT buffer ...
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bioRxiv - Biophysics 2024Quote: ... Digest the DNA with DraIII-HF restriction enzyme (NEB R3510L) for 3 hr at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and was loaded onto 2 mL of chitin resin (NEB; #S6651S) pre-equilibrated with lysis buffer ...
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bioRxiv - Genomics 2024Quote: ... cDNA was PCR amplified using the NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs, Cat. No. M0541) with the following forward and reverse primers ...
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bioRxiv - Biophysics 2024Quote: ... and 1 µL of BaeGI-HF (NEB; 100,000 units/mL) and incubated for 1 h at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... Tagmented DNA was PCR amplified using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs, Cat. No. M0541) with indexed primers (CAAGCAGAAGACGGCATACGAGATNNNNNNNNNNGTCTCGTGGGCTCGG and AATGATACGGCGACCACCGAGATCTACACNNNNNNNNNNACACTCTTTCCCTACACGACGCTCTTCCGATCT ...
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bioRxiv - Genomics 2024Quote: ... Assembled product was transformed into electrocompetent cells (NEB, Cat. No. C3020K) and plasmid DNA was extracted with a midiprep kit (Zymo Research ...
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bioRxiv - Genomics 2024Quote: ... and Template Switching RT Enzyme Mix protocol from NEB (New Engand Biolabs, Cat. No. #M0466L). cDNA was PCR amplified using the NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... and BbsI (NEB, Cat. No. R0539S) in 10X NEBuffer r2 at 37 degrees Celsius overnight to ensure complete digestion of the backbone ...
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bioRxiv - Genomics 2024Quote: The 493 gRNAs with associated 10N random barcodes were ordered as an IDT oPool and PCR amplified with Q5 High-Fidelity polymerase (NEB, Cat. No. M0491S) to make double stranded DNA ...