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Citations for New England Biolabs :
3901 - 3950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 10^106 of DMSO or IMP-1088 released virions were treated with two-fold dilutions of Furin (New England Biolabs, Cat. no. P8077L) in 20 mM HEPES ...
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2-specific amplicons were generated using xGen Artic V4 NCoV-2019 primers (Integrated DNA Technologies Inc., Coralville, Iowa) and Q5 Hot Start High Fidelity PCR Master Mix (New England Biolabs, Cat. no. E0555S). Sequencing libraries were prepared using NEBNext ultra II DNA library kit (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were prepared using NEBNext ultra II DNA library kit (New England Biolabs, Cat. no. E7805S) and unique dual indexes (Integrated DNA Technologies Inc. ...
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bioRxiv - Microbiology 2024Quote: ... RNA concentration and quality were evaluated using NanoDrop 2000 Spectrophotometer (Thermo Fischer Scientific) and reverse-transcribed to cDNA using LunaScript® RT SuperMix Kit (New England Biolabs, Cat no. E3010) with random hexamers and oligo dT ...
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bioRxiv - Immunology 2024Quote: ... Stranded RNA sequencing libraries were prepared as described using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (Cat# E7760L; New England Biolabs). Purified libraries were qualified on an 4150 TapeStation (Agilent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: Gibson Assembly (NEB #E2611) was used to clone sgRNAs sequences derived from the GeCKO v2 library per gene of interest (NTN1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting gel purified fragment was quantitated by Qubit and cloned into the lentiviral vector LentiCRISPR-V2 previously digested with BsmBI (New England Biolabs, # R0739) as described (65) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were extracted from agarose using a Monarch DNA Gel Extraction Kit (NEB, # T1020), quantitated and next generation sequencing was performed using an Illumina NextSeq 75 cycle High Output kit on an Illumina NextSeq platform.
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bioRxiv - Cancer Biology 2024Quote: ... PCR amplification and barcoding performed as described (Hart paper, Cell, 2015) using NEBNext Q5 Ultra mastermix (NEB, # MO544). PCR products were extracted from agarose using a Monarch DNA Gel Extraction Kit (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... The spheres were collected after 24 h and RNA extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs #T2010S). RNA was extracted from adherent cells directly from the plate ...
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bioRxiv - Biophysics 2024Quote: ... and in 1 × CutSmart buffer (NEB). The reaction mixture was pipetted ten times to ensure thorough mixing ...
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bioRxiv - Biophysics 2024Quote: ... In the first double-digestion reaction two DNA fragments of 3,621 nt (D) and 3,628 nt (D’) were generated using DraIII and BaeGI enzymes (New England Biolabs; Figure S1a). The digestion reaction involved annealing two oligonucleotides (Figure S1b ...
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bioRxiv - Biophysics 2024Quote: ... the mixture was purified using the Monarch PCR and plasmid DNA purification kit (NEB), eluted in 10 mM Tris-HCl pH 8.0 and stored at -20 °C for further experiments ...
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bioRxiv - Biophysics 2024Quote: ... 55 The samples were mixed with 6 × purple loading dye without sodium dodecyl sulfate (NEB) and with 10 × TBE to make a 1 × solution ...
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bioRxiv - Biophysics 2024Quote: ... EcoRV (New England Biolabs) cleavage was performed in CutSmart buffer overnight ...
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bioRxiv - Biophysics 2024Quote: ... In vitro pull-down binding assays were performed in triplicate by incubating proteins at their indicated concentrations with 20 µL amylose resin (bead bed volume; New England Biolabs) in a 200 µL reaction in Assay Buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were then subjected to amylose resin (E8021; NEB, Frankfurt, Germany) in buffer D (20mM Tris pH=7.4 ...
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bioRxiv - Microbiology 2024Quote: ... 20 pmoles of dephosphorylated RNA was combined with 2 μl 10 x T4 Polynucleotide Kinase buffer (NEB), 3 μl ATP(y-32P ...
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bioRxiv - Microbiology 2024Quote: In vitro transcribed CHIKV RNA (nt’s 1-337) was 5’ dephosphorylated using Quick CIP according to the manufacturer’s instructions (NEB) before purification using an RNA Clean & Concentrator column (Zymo Research) ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were prepared using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England Biolabs; #E6177) per manufacturers protocol with 50-100 ng of DNA input and 5-6 cycles of PCR ...
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bioRxiv - Microbiology 2024Quote: ... was conducted after phosphorylating the 5’ end of the pooled fragments with T4 PNK (NEB, #M0201S). Reverse transcription and PCR amplification of the library were followed by PAGE-assisted size selection were carried out as previously outlined67 ...
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bioRxiv - Biophysics 2024Quote: ... coli (NEB cat. # C2528J). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... Supernatant was loaded onto an amylose resin column (New England Biolabs) equilibrated with binding buffer (25 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... 100 ng or less template DNA and 24 μL Taq DNA polymerase (NEB M0273L). Purify and concentrate the PCR DNA product by ethanol precipitation ...
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bioRxiv - Cancer Biology 2024Quote: ... All single-cell libraries were prepared using the NEBNext Ultra II FS kit (New England Biolabs) following the manufacturer’s instructions but using one-fourth volumes ...
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bioRxiv - Cancer Biology 2024Quote: ... Unique dual index primer pairs (New England Biolabs) were used to barcode single -cell libraries ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was collected from parental iOvCa147 cells and DYRK1A-/-cells from 24-hour adherent or 6-hour spheroid conditions using the Monarch Total RNA Miniprep Kit (NEB #T2010S) as described above ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). These libraries were subsequently sequenced for paired-end reads of 2×100 bp on an MGI system at a sequencing depth of 50X.
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bioRxiv - Cancer Biology 2024Quote: Isolated total RNA was processed using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, MA, USA) to isolate intact poly(A)+ RNA following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... with 1 µg of total RNA used for sequencing library construction with NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). Raw FastQ files were mapped using Bbsplit from the Bbtools 39.01 suite against Mm10 and hg38 (31 ...
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bioRxiv - Biophysics 2024Quote: ... 25μg of the plasmid containing the hsp70 promoter sequence was digested with HindIII restriction enzyme (NEB R3104) for 3 hours at 37C ...
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bioRxiv - Biophysics 2024Quote: ... followed by Amylose resin (NEB cat. # E8021S) and heparin affinity column (Cytiva cat ...
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bioRxiv - Biophysics 2024Quote: ... and AfeI (NEB; 10,000 units/mL) instead ...
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bioRxiv - Biophysics 2024Quote: ... but employed restriction endonucleases DrdI (NEB; 10,000 units/mL) and AfeI (NEB ...
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bioRxiv - Biophysics 2024Quote: ... A 1 kbp ladder (NEB) was used for the relative comparison of the bands.
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bioRxiv - Biochemistry 2024Quote: ... the ecpga gene (GenBank: X04114.1) was amplified by PCR using Q5 polymerase (NEB, USA) from our previous plasmid pKA1868 with ecPGA cloning primers bearing NdeI and XhoI site overlaps ...
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bioRxiv - Biochemistry 2024Quote: ... and digested PCR product by NdeI and XhoI restriction enzymes (NEB, USA) was ligated using T4 DNA ligase (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... The parental vector was digested by the DpnI enzyme (NEB, USA), and the ligation mixture was directly electroporated into E ...
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bioRxiv - Biochemistry 2024Quote: ... These fragments were cloned into linearized pUC19 using the Hi-Fi Assembly Cloning Kit (New England Biolabs). The plasmids carrying the desired inserts were transformed into strains by biolistic transformation as previously reported (82) ...
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bioRxiv - Biochemistry 2024Quote: ... was ligated using T4 DNA ligase (NEB, USA) into the pET-26b vector cleaved with the same enzymes ...
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bioRxiv - Biochemistry 2024Quote: ... These fragments were cloned into linearized pUC19 using the Hi-Fi Assembly Cloning Kit (New England Biolabs). The tps1Δ mutant was confirmed by PCR and the TPS1IDDΔ strain was confirmed by PCRs and sequencing to confirm the replacement of the CnTps1IDD with the CaTps1IDD ...
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bioRxiv - Microbiology 2024Quote: ... Repair template was generated by using oligos P7/P8 to amplify GFP sequence from plasmid pCas9/sgRNA/Bleo with Phusion® High-Fidelity DNA Polymerase (New England Biolabs; #M0530S). ME49Δku8023 was transfected with 100 μg of gRNA and 20 μg of repair template ...
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bioRxiv - Microbiology 2024Quote: ... using Q5® Site-Directed Mutagenesis Kit (New England Biolabs; #E0554S). Guide RNA and repair template sequences are listed in Supplementary Data 3c.
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bioRxiv - Microbiology 2024Quote: ... digested with BamHI and PstI restriction endonucleases according to the manufactureŕs instructions (New England Biolabs), and ligated into the pMAL-p2E vector digested with the same restriction endonucleases using the T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... The piggyFlex backbone vector was digested with SalI (NEB, Cat. No. R3138S) and BbsI (NEB ...
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bioRxiv - Genomics 2024Quote: ... and the linear backbone vector (5098 base pairs in size) was gel extracted using a gel extraction kit (NEB, Cat. No. T1020S). The second product from the digestion (2878 base pairs ...
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bioRxiv - Genomics 2024Quote: ... and Template Switching RT Enzyme Mix protocol from NEB (New Engand Biolabs, Cat. No. #M0466L). cDNA was PCR amplified using the NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... The resulting vector was then digested with SapI (NEB, Cat. No. R0569S), for one hour at 37 degrees Celsius ...
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bioRxiv - Genomics 2024Quote: ... The PCR amplified IDT oPool gRNAs with associated 10N random barcodes were cloned into the linear backbone using NEBuilder HiFi DNA Assembly (NEB, Cat. No. E2621S) using 0.15 pmol of the insert (gRNA library ...
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bioRxiv - Molecular Biology 2024Quote: ... using ETAA1 DBR R1 RT-PCR primer 5′-AAGTTCTTCTTCTTGACTTTGTGTT-3′ and treated with RNaseH (New England Biolabs, M0297S). 1μl of the cDNA was used for PCR amplification reactions using using GoTaq® G2 DNA Polymerase (Promega ...