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Citations for New England Biolabs :
3801 - 3850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... gDNA extraction was performed using a Monarch Genomic DNA Purification Kit (NEB, T3010S) following the Manufacturer’s protocol for cultured cells with an input of 5e6 HeLa cells ...
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bioRxiv - Genomics 2024Quote: ... coli Poly(A) Polymerase (NEB, M0276S) using 12 μg of input RNA ...
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bioRxiv - Genomics 2024Quote: ... and purified using a Monarch RNA Cleanup Kit (NEB, T2040S), both according to the Manufacturer’s protocols ...
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bioRxiv - Genomics 2024Quote: ... coli (derived from NEB C3040 and prepared according to Nováková et al.14 ...
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bioRxiv - Genomics 2024Quote: ... the PCR reaction was set up with Q5 polymerase (NEB, M0491L). Thermocycling conditions were as follows ...
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bioRxiv - Microbiology 2024Quote: ... RNA libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) and sequenced using a NextSeq 550 75 Cycle High Output kit for single-end sequencing.
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bioRxiv - Genomics 2024Quote: ... treated with USER enzyme (NEB) and amplified by PCR with barcoded universal primers NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50uL reactions were prepared using 1x Q5 Hotstart Master mix (NEB M0494S), 0.5uM Forward primer ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µl of Golden Gate Enzyme Mix (BsaI-HFv2, NEB M2616AA), and adding dH2O to reach a total volume of 20 µl ...
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bioRxiv - Systems Biology 2024Quote: ... 2 μL of T4 DNA ligase (NEB) were added and incubated at RT for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... 1x Phusion GC buffer (NEB), 0.2 mM dNTPs ...
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bioRxiv - Genomics 2024Quote: ... T4 PNK (NEB # M0201) and Klenow DNA Polymerase (NEB # M0210) ...
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bioRxiv - Genomics 2024Quote: ... DNA was digested into single nucleosides by using the Nucleoside Digestion Mix (New England Biolabs) enzyme cocktail at 37°C for 2 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic libraries were prepared with NEBNext Ultra II FS DNA Library Prep with Beads (New England Biolabs), with single indexes ...
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bioRxiv - Neuroscience 2024Quote: ... linearized with XbaI (NEB) and purified via DNA extraction kit (ELPIS) ...
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bioRxiv - Plant Biology 2024Quote: ... and Amylose Resin (New England Biolabs, Ipswich, MA, USA) affinity chromatography ...
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bioRxiv - Neuroscience 2024Quote: ... 1% (v/v) BSA (20 mg/ml, NEB, B9000S) + 5μL of hash oligo (10 uM ...
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bioRxiv - Microbiology 2024Quote: ... 20 U of DpnI (New England Biolabs : R0176S) was added to the PCR products and incubated at 37 °C for 1 hour to remove the parental PCR DNA template ...
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bioRxiv - Microbiology 2024Quote: pRS31N-Barcode was digested with NdeI (NEB : R0111S) for 2 hours at 37°C and inactivated for 20 minutes at 65°C:
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bioRxiv - Microbiology 2024Quote: ... Here amplicons were incubated with T4 polynucleotide kinase (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Quick Ligase kit (M2200, NEB, Ipswich, US). For a site-directed mutagenesis the central guanine of the seed sequence was exchanged for an adenosine ...
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bioRxiv - Pathology 2024Quote: Sequencing library was generated from total mRNA (1 µg) using NEBNext UltraTM RNA Library Prep Kits for Illumina (New England BioLabs, MA). cDNA libraries were sequenced by a NovaSeq 6000 (Illumina ...
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bioRxiv - Systems Biology 2024Quote: ... Vectors were digested using restriction enzymes (EcoRI and BamHI) and PCR fragments were amplified using Q5 DNA polymerase (NEB). NES-APEX2 ...
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bioRxiv - Systems Biology 2024Quote: ... and A-tailed using Klenow HC 3′ → 5′ exo (#M0212L; NEB).
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bioRxiv - Systems Biology 2024Quote: ... Barcodes were digested overnight with 80 units of NheI (#R0131S; NEB) and purified by magnetic bead purification (#CPCR-0050 ...
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bioRxiv - Systems Biology 2024Quote: ... Linear barcoded vectors were purified by magnetic beads and digested for 3h with 40 units of XcmI (#R0533S; NEB). Finally barcoded vectors were purified again by gel-purification.
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bioRxiv - Systems Biology 2024Quote: ... each library was digested overnight with I-CeuI (#R0699S, NEB), barcode-insert fragments were then circularized ...
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bioRxiv - Systems Biology 2024Quote: Each of the selected eight promoters were amplified by PCR and individually inserted by Gibson assembly (#E2611S; New England Biolabs) into the reporter vector ...
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bioRxiv - Molecular Biology 2024Quote: ... sample was washed with 1x T4 ligase buffer (NEB) (2,500 xg ...
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bioRxiv - Cell Biology 2024Quote: ... was inserted into murine Shh between amino acids 92N and 93T (corresponding to N91 and T92 in human Shh) by using Gibson assembly (HiFi Assembly Kit, NEB). Where indicated ...
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bioRxiv - Bioengineering 2024Quote: ... both from NEB and all work in E ...
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bioRxiv - Cancer Biology 2024Quote: ... 5ng of cDNA were amplified with the long LongAmp Taq 2X Master Mix (New England Biolabs) for 25 cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... modified with an EF1a promoter and a Basticidin resistance gene using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Specific primers and NEBuilder HiFi DNA Assembly Master Mix were used to introduce the exon E6a or the R238W mutation into the canonical sequence of NT5C2.
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bioRxiv - Biophysics 2024Quote: ... using Gibson Assembly (NEB E5520S) to contain an additional N-terminal cysteine for fluorophore labeling ...
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bioRxiv - Cell Biology 2024Quote: ... and NdeI (60U, NEB, R011S). 20 ng of cut DNA was ligated to the 42-mer and 11-mer as described previously (66) ...
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bioRxiv - Neuroscience 2024Quote: ... 5 μl Hot Start High-Fidelity 2× Master Mix (M0494S; NEB) and nuclease-free water to fill up to 10 μl ...
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bioRxiv - Genomics 2024Quote: ... The 3 library pools were purified by an ExoSAPII (NEB) reaction to remove single-stranded DNA and further by column purification (MiniElute Gel Extraction Kit ...
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bioRxiv - Biochemistry 2024Quote: PNGase F (New England Biolabs) was used to remove N-glycan moieties from proteins under denaturing conditions according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... in a black-bottomed 96-well plate and gene-specific LA qPCR assays were performed as described earlier18,19,20,27,47,48 using Long Amp Taq DNA Polymerase (New England BioLabs). Three transcribed (HPRT ...
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bioRxiv - Cell Biology 2024Quote: ... the Bmal1:luc reporter vector was digested with BamHI (NEB R3136S), supplemented with calf intestinal alkaline phosphatase (Quick CIP ...
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bioRxiv - Cancer Biology 2024Quote: ... poly-A mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB; E7490) and subsequently used to assemble the RNA-seq library using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... PCR reactions were performed with Q5 polymerase (New England Biolabs) in the buffer provided ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... of the Oprl1 gene were amplified from a C57BL/6 BAC clone by PCR using Q5 Polymerase (New England Biolabs) and cloned into polylinkers of a targeting construct that contained IRES-mnCre:GFP ...
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bioRxiv - Microbiology 2024Quote: ... truncated K227Q (NEB; #M0315S). RNA and RPF samples were each pooled together after PAGE-assisted size selection ...
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bioRxiv - Microbiology 2024Quote: ... and ligated into the pMAL-p2E vector digested with the same restriction endonucleases using the T4 DNA Ligase (New England Biolabs) according to the manufactureŕs instructions ...
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bioRxiv - Microbiology 2024Quote: ... Ligation of 5’ linker using T4 RNA ligase 1 (NEB; #M0204S) was conducted after phosphorylating the 5’ end of the pooled fragments with T4 PNK (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and mixed with 250 μl of Amylose resin (New England Biolabs) for 2 hours at 4°C in an orbital shaker ...
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bioRxiv - Microbiology 2024Quote: ... The plasmid was transformed into BL21 (DE3) competent cells following the manufacturer’s protocol (NEB), a single colony was inoculated into 10 mL LB ampicillin (10 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... 1 μl T4 Polynucleotide Kinase (NEB) and nuclease free H2O to a final volume of 20 μl ...
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bioRxiv - Physiology 2024Quote: ... The insoluble matrix samples were resuspended in in 1.5x Laemmli buffer with DTT (New England Biolabs) in a normalized volume based on cytosolic protein concentration (50 μl per mg of soluble protein) ...