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Citations for New England Biolabs :
3751 - 3800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... libraries were suspended and amplified (NEBUltra Q5 polymerase (New England Biolabs GmbH, Frankfurt, Germany), IDT xGen (IDT ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... one half was collected in a 1.5 mL tube containing 300 µL of 1x RNA Protection Reagent (NEB #T2011-1) and snap-frozen ...
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bioRxiv - Developmental Biology 2024Quote: ... a total of 1μg of the sgRNA pair along with 20 pmol recombinant Cas9 (EnGen S. pyogenes Cas9 NLS from NEB, M0646T) were injected into the C ...
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bioRxiv - Developmental Biology 2024Quote: ... a pair of single guide RNAs (sgRNAs) were synthesized using the EnGen sgRNA Synthesis Kit (E3322V) from NEB; a total of 1μg of the sgRNA pair along with 20 pmol recombinant Cas9 (EnGen S ...
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bioRxiv - Developmental Biology 2024Quote: ... Phusion DNA Polymerase (NEB, M0530S), 10mM dNTPs (Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... oclnb-GFP BAC was linearized with PvuI enzyme (NEB), which cuts 110 kb upstream of occludin b gene coding region ...
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bioRxiv - Developmental Biology 2024Quote: ... 2020) and 5’Ncol1::mScarlet::3’Ncol119 plasmids using Gibson assembly according to the manufacturer recommendations (NEB, CAT#E2611). Sequences of Gibson primers used were:
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bioRxiv - Developmental Biology 2024Quote: ... The RNA-sequencing (RNA-seq) libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, E7420L), The yield of cDNA libraries was measured using the Qubit dsDNA HS Assay Kit ...
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bioRxiv - Developmental Biology 2024Quote: ... following the manufacturer’s guidelines provided with the library kit (E7560S, New England BioLabs Inc., USA). Monarch PCR purification kit (Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... Monarch PCR purification kit (Biolabs, New England) used to purify the resulting amplified cDNA constructs ...
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bioRxiv - Genetics 2024Quote: ... Cas9H840A was made by site-directed mutagenesis of pLS504 using the Q5 site-directed mutagenesis kit (New England Biolabs #E0554S) using primers MK118 and MK119 to yield pLS517 ...
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bioRxiv - Genetics 2024Quote: ... Gibson Assembly was performed using the master mix (New England Biolabs, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... coli cells for each replicate (New England Biolabs).
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bioRxiv - Genetics 2024Quote: ... following a digestion of the plasmid with SpeI-HF (New England Biolabs).
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bioRxiv - Genetics 2024Quote: ... and inserted into MSp14 via Gibson assembly (New England Biolabs, Ipswich, MA, USA) following a digestion of the plasmid with SpeI-HF (New England Biolabs).
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bioRxiv - Genetics 2024Quote: ... and inserted by Gibson assembly into MSp11 following a double digestion with SphI-HF and BstEII-HF (New England Biolabs). This plasmid was named MSp501.
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bioRxiv - Genetics 2024Quote: ... MSp218 was double digested using EcoRI-HF and BamHI-HF (New England Biolabs) on the cloning site ...
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bioRxiv - Genetics 2024Quote: ... the NEB Ligation Buffer in the ligation step was substituted by NEB rCutSmart buffer (Cat#B6004) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We added 1 µL of ApaLI (NEB #R0507L) and 5.5 µL of Cutsmart Buffer (NEB #R0507L ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and 5.5 µL of Cutsmart Buffer (NEB #R0507L) to genomic DNA and incubated at 37°C for at least 1hr ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 30 µL of Q5 buffer (NEB #M0491L), and 65.5 µL of nuclease free water ...
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bioRxiv - Evolutionary Biology 2024Quote: ... adding 3.5 µL of Cutsmart buffer and 1 µL of ApaLI restriction enzyme (NEB #R0507L) to each sample’s Step 2 PCR product ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1.5 µL of Q5 polymerase (NEB #M0491L), 30 µL of Q5 buffer (NEB #M0491L) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and 200 µL of OneTaq HotStart 2X Master mix (NEB #M0484L). Three cycles of PCR was then carried out with the following steps:
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bioRxiv - Evolutionary Biology 2024Quote: ... or Phusion HF DNA polymerase (NEB) expression analysis.
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR was carried out with One Taq DNA polymerase (NEB, cloning) using primers listed in Supplementary Table 5 and products cloned using the pGEM-T Easy Vector System I (Promega ...
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bioRxiv - Developmental Biology 2024Quote: ... The libraries were amplified using NEB Next High- Fidelity 2X PCR Master Mix (NEB) with primer extension at 72°C for 5 min ...
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bioRxiv - Genetics 2024Quote: ... the left and right homology arms were amplified by pfusion PCR (NEB) using y w genomic DNA as template and primer pairs Ti897/898 and Ti899/900 ...
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bioRxiv - Genetics 2024Quote: ... TLK1 and TLK2 point mutants were generated by site-directed mutagenesis reactions using the NEB Q5 site-directed mutagenesis kit (NEB, E0554S). Primers are listed in Supplementary Table 1.
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bioRxiv - Genetics 2024Quote: ... and treated with CIP (New England BioLabs) to generate pJM10 ...
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bioRxiv - Genetics 2024Quote: ... Each assay was prepared to contain a final concentration of 1× PrimeTime Master Mix (IDT) or 1× Luna Universal qPCR Master Mix (NEB), 0.1 μM for each of the two probes (LNA9K1-Gly ...
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bioRxiv - Genetics 2024Quote: ... and SV40 polyA PCR products were assembled in pattB-w-using Gibson assembly (NEB). Act5C-sfGFP and 3xP3-sfGFP integrations into attP40 were recovered by BestGene ...
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bioRxiv - Genetics 2024Quote: ... along with an additional 5 µL 2x Gibson Assembly Master Mix (NEB Cat#E2611L). The reaction mixture was incubated at 50°C for 1 hour ...
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bioRxiv - Genetics 2024Quote: ... YCp50 was digested using restriction enzymes EcoRI-HF (NEB Cat#R3101S) and BspDI (NEB Cat#R0557S ...
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bioRxiv - Genetics 2024Quote: ... and BspDI (NEB Cat#R0557S) with rCutSmart buffer ...
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bioRxiv - Genetics 2024Quote: ... coli (NEB Cat#C2987I) and plated onto 10-cm LB-AMP plates (IPM Scientific Cat#11006-016 ...
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bioRxiv - Genetics 2024Quote: ... ProtoScript II (NEB), and 1 μg of total RNA input.
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bioRxiv - Immunology 2024Quote: ... 0.4 µl of 2000 U/ml Phusion® High-Fidelity DNA Polymerase (NEB, #M0530S), 2 µl of the universal forward primer (5’-AAGCAGTGGTATCAACGCAGAG-3’ ...
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bioRxiv - Cell Biology 2024Quote: ... we microinjected 5 ng/μl Pmyo-3::GFP and 95 ng/μl of 2-Log DNA ladder (New England BioLabs) into the germline of GRD-3::mKate2 animals to generate transgenics expressing a muscle-specific GFP marker.
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bioRxiv - Cell Biology 2024Quote: ... which were generated via PCR using the Q5 DNA Polymerase (New England BioLabs), purified with HighPrep PCR Clean-up beads (MagBio) ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to manufacturer’s directions ...
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bioRxiv - Cell Biology 2024Quote: ... followed by NEB NEBNext Ultra II (NEB #E7760) or Illumina TruSeq library preparation and Illumina platform sequencing ...
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bioRxiv - Microbiology 2024Quote: ... PCR-amplified DNA fragments were assembled by Gibson assembly kit (NEBuilder, NEB) as per manufacturer’s protocol and introduced into DH5α E ...
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bioRxiv - Microbiology 2024Quote: ... coli Poly(A) Polymerase for 30 min following the manufacturer’s protocol (NEB, Ipswich, MA, USA). Polyadenylation levels were assessed with Bioanalyzer chip gel electrophoresis and through evaluation of a size shift increase of ∼500 bp for the 23S and 16S rRNAs ...
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bioRxiv - Microbiology 2024Quote: ... Chromosomal integration of fluorescent tags was confirmed by colony PCR (touchdown protocol) using Q5 HF Master mix (NEB, USA). The primer pair Fwd_full series_Tn7 with Rev_Tn5/7_gt were used to confirm the integration of fluorescent tags ...
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bioRxiv - Genomics 2024Quote: ... Coli (NEB), purified using the Zyppy Plasmid Miniprep Kit (Zymo) ...
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bioRxiv - Genomics 2024Quote: ... The plasmid was digested with Esp3I (NEB), while the PCR amplicon was digested with BsaI (NEB) ...
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bioRxiv - Genomics 2024Quote: ... cloning was performed using PCR of desired insert fragments with Q5 (New England Biolabs (NEB)) ...
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bioRxiv - Genetics 2024Quote: ... the proteinase K (NEB) was added and incubated for 2 h at 55 °C to digest protein and the RNase A (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... HINDIII ((20,000 U/mL) (Catalog: R3104S, New England Biolabs, Ipswich, MA, USA) restriction enzyme ...