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Citations for New England Biolabs :
151 - 200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... while library concentration was determined using the NEBNext Library Quant Kit for Illumina (NEB, Cat# E7630). All input RNA had RNA integrity number (RIN ...
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bioRxiv - Molecular Biology 2024Quote: ... utilizing the NEBNext Poly (A) mRNA Magnetic Isolation Module (NEB, Cat# E7490) to enrich mRNA and the Ultra II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... and dephosphorylated by Quick CIP (NEB, Cat# M0525S). Then ...
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bioRxiv - Microbiology 2024Quote: Polynucleotides were synthesized by PCR using Phusion High-Fidelity DNA polymerase (NEB) and locus-specific primers (see Table S2 for sequences) ...
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bioRxiv - Cell Biology 2024Quote: ... The purified DNA was digested overnight with SbfI-HF (New England Biolabs) and separated on a 0.8% TAE agarose gel ...
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bioRxiv - Cell Biology 2024Quote: ... and AgeI-HF (R3552S-NEB). The PCR fragment was subsequently purified using the Wizard SV Gel and PCR Clean-Up System (A9281 - Promega ...
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bioRxiv - Cell Biology 2024Quote: ... The amplicon was restriction digested with EagI (New England Biolabs) and XhoI (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... The amplicon was restriction digested with SacI (New England Biolabs) and EagI ligated into pRS406 linearized with SacI and EagI ...
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bioRxiv - Cell Biology 2024Quote: ... was amplified using Q5 high-fidelity DNA polymerase (New England Biolabs, Whitby, ON, Canada) and primers are listed in Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... in comparison to low molecular weight ssRNA (NEB #N0364S). Gels were cut on a blue light box corresponding to the size of tRNAs (70 to 100 nt ...
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bioRxiv - Microbiology 2024Quote: ... samples were PCR amplified using HF Phusion enzyme (NEB #M0530L) (2 μl of cDNA ...
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bioRxiv - Microbiology 2024Quote: ... DNase-treated total RNA was further treated with RNA 5’ Pyrophosphohydrolase (New England Biolabs) to remove possible pyrophosphate from the 5’ end ...
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bioRxiv - Microbiology 2024Quote: All PCR reactions were performed using Phusion HF polymerase (New England Biolabs). All oligonucleotides were purchased from Integrated DNA Technologies (IDT) ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs, #E7760) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Libraries of small RNA were constructed using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England BioLabs, #E7330) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... or Induro (NEB #M0681L) reverse transcriptases ...
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bioRxiv - Cell Biology 2024Quote: All G3BP paralogs (G3BP1, G3BP2A, and G3BP2B) cDNAs were cloned by Gibson assembly (NEB, E2611L) into either an mEGFP-C1 (Addgene ...
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bioRxiv - Microbiology 2024Quote: ... Size-selected cDNA was then PCR amplified for 12 cycles using Q5 High-fidelity polymerase (NEB #M0491S). Amplified libraries were then run on a 6% TBE gel ...
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bioRxiv - Microbiology 2024Quote: ... and cells were resuspended in 1 X DNAse buffer (New England Biolabs) and then treated with DNAse (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... the NEB OneTaq® One-Step RT-PCR Kit (New England Biolabs, Product # E5315S) was used to target the haemagglutinin gene ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg of DNA was mixed with 7 µl NEBNext Ultra II end prep reaction buffer (NEB) and 3 µl Ultra II end prep enzyme mix (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... and then treated with DNAse (New England Biolabs) for 30 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... the NEBNext ultra II ligation module (NEB). After ligation ...
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bioRxiv - Microbiology 2024Quote: ... thermophilus extracts or PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, Ipswich, MA, USA) and the reactions were further incubated at either 50 °C or 37 °C for another 4 h in the case of T ...
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bioRxiv - Microbiology 2024Quote: Cell-free transcription and translation reactions were carried out using PURExpress® In Vitro Protein Synthesis Kit (NEB) following the manufactureŕs protocol ...
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bioRxiv - Microbiology 2024Quote: ... DNA libraries were constructed using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... Each gene was PCR amplified from the plasmids using Q5 polymerase (New England Biolabs; NEB) with the primers 5’-GAAGTGCCATTCCGCCTGACC and 5’-CACTGAGCCTCCACCTAGCC ...
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bioRxiv - Microbiology 2024Quote: Complimentary DNA (cDNA) was prepared from 250 ng of total RNA using the First Strand cDNA Synthesis Kit (New England BioLabs Inc., USA), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 uL each 10 uM forward and reverse primers (cTF256, cTF257) and 25 uL NEBNext Ultra II Q5 Master Mix (NEB, M0544) and ran the following thermocycling protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Each methylation reaction was thoroughly mixed on ice using 100uL of cell methylation buffer (10% volume 10x M.CviPI reaction buffer (NEB, B0227S), 300 mM sucrose ...
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bioRxiv - Molecular Biology 2024Quote: ... coli (NEB, C30131). 10 mL overnight cultures were diluted into 1 mL LB medium with 50 µg/mL kanamycin (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... End prep and adaptor ligation was performed using the Enzymatic Methyl-seq kit (NEB). Conversion was performed identically to the amplicons ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1 μL of T7 Ligase (New England Biolabs, M0318S), 0.4 μL of Fermentas FD Eco31I (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The CHD4-GFP mutant was cloned with selective primers (fwd, 5’-GGATGCTACAGGTGGAACCCTGCACCCCTA-3’; rev, 5’-GCCCAGGCCCGACGCCAT-3’) and a Q5 site-directed mutagenesis kit (NEB) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries for DNA fragments were prepared based on the NEBNext Ultra II DNA library prep Kit for Illumina (NEB#E7645).
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bioRxiv - Molecular Biology 2024Quote: ... re-digestion with BamHI (NEB) and purification with a PCR clean-up kit (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... purified DNA fragments were subjected to library preparation with NEB Next Ultra II (NewEngland Biolabs, E7645L) and NEB MultiplexOligo sets I/II/III/IV (NewEngland Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2.5 µL 10x CutSmart buffer (NEB) were added and incubated for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... template was generated from pCRII-TOPO-NEAT1_1 (kind gift from Archa Fox) by double digestion with BamHI/NcoI (NEB), PCR amplification using Phusion polymerase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries for small DNA fragments (25-75 bp) were prepared based on the NEBNext Ultra II DNA library prep Kit for Illumina (NEB#E7645).
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bioRxiv - Molecular Biology 2024Quote: Total RNA (5 µg in 20 µL ddH2O) was incubated with 2µL 100U Nuclease P1 (NEB) and 2 µL 10x Nuclease P1 buffer (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µL RNase H (5 U/µL, NEB), 2.5 µL RNase III (1 U/µL ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA substrate was incubated with 1 unit of purified Uracil-DNA glycosylase (New England BioLabs) instead of cell extracts in the same reaction buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Coli UDG (New England Biolabs Inc. ...
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bioRxiv - Molecular Biology 2024Quote: ... 25ul ChIP DNA or 10ng Input DNA were used to generate libraries using the NEBNext Ultra II Library Prep Kit for Illumina (NEB). Reactions were scaled down to half otherwise processing was according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2024Quote: ... The repair template was assembled and integrated into pCC1 using Gibson assembly (Gibson et al., 2008) (NEB Hifi assembly kit). Colony PCR was used to check the plasmids had the correct inserts (F-93/R-91 ...
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bioRxiv - Molecular Biology 2024Quote: ... for each guide a pair of primers were designed which when annealed produce the guide sequence with overhangs matching those produced when pDC2 was digested with bbsI (New England Biolabs, USA), allowing for ligation to occur ...
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bioRxiv - Molecular Biology 2024Quote: ... A repair template plasmid was constructed using Gibson assemble (Gibson et al., 2008) (NEB Hifi assembly kit). The repair construct was made by joining the PfAMA1 coding region and ∼1.5 kb 5’ to the transcription start site amplified from 3D7 genomic DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Q5 High Fidelity 2X Master Mix (New England Biolabs, M0492).
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bioRxiv - Molecular Biology 2024Quote: ... an in vitro transcribed sgRNA (EnGen, NEB), and recombinant CAS9 was done using Lonza’s P1 kit ...