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Citations for New England Biolabs :
3701 - 3750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... were assembled using NEBulider HiFi DNA Assembly Mix (New England Biolabs, catalog no. E2621L). These constructs were then cloned into PCR4-Shh:LacZ-H11 plasmids (Addgene plasmids ...
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bioRxiv - Cell Biology 2024Quote: ... and ligated with the oligo solution according to the NEB Quick Ligase (NEB, M2200) protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The partial AtVPS13M1 gene was amplified by PCR in 45 cycles using LongAmp Hot Start Taq 2X Master Mix (New England Biolabs), from 30/40ng of cDNA per sample in reaction volume of 100µl ...
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bioRxiv - Cell Biology 2024Quote: ... together with the NEBNext Poly(A) mRNA Magnetic Isolation Module (#E7490) and NEBNext Multiplex Oligos for Illumina (#E7600) (New England Biolabs). RNA-seq libraries were processed on a Biomek i7 Hybrid instrument (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... the sequence encoding a sgRNA targeting the 3’ end of arkx was assembled by annealing two complementary oligonucleotides (gRNA_ARKx_a and gRNA_ARKx_b) and cloned into the BsaI-digested pHF-gC backbone (9) using T4 DNA ligase (New England Biolabs #M0202). The donor plasmid pD_ARKx_icKD was produced by assembling four DNA fragments in a Gibson Assembly reaction (77 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we obtained the methylated and non-methylated counts at individual CpG sites of the reference genome (hg38) in germline cells using genome-wide methylation data originating from NEBNext Enzymatic Methyl-seq (EM-seq; New England Biolabs, Ipswich, MA, USA) of flow- sorted spermatogenic cell types representing 4 different stages of spermatogenesis ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with 15 µl of anti-MBP couped beads (pre-blocked for 1h in RIPA with 3 % BSA; NEB) for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... and 50 U ApoI-HF (NEB) together with 0.187 µM annealed mutREAD adapters (see Key resource table) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with an imaging medium that contained 40 - 80 nM SNAP Cell 647 Sir dye (NEB, S9102S) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... or BL21 (DE3) (New England Biolabs) chemically competent E ...
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bioRxiv - Cancer Biology 2024Quote: ... vectors were transformed into T7 Express lysY competent cells (New England Biolabs). For histone H4 expression ...
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bioRxiv - Cell Biology 2024Quote: ... and BamHI-HF (NEB R3136) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... In vitro transcription was carried out with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs), followed by the template DNA digestion with DNase I (Takara Bio) ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells transfected with Apaf1-SNAP-tag were stained with 1.5 nM SNAP-Cell 647-SiR substrate (NEB, S9102S), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... MEF cells expressing H3.1-SNAP and H3.3-SNAP were first blocked using SNAP-Cell Block (New England Biolabs, S9106S) to inhibit the preexisting fractions/populations of H3.1-SNAP-3XHA and H3.3-SNAP-3XHA ...
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bioRxiv - Cell Biology 2024Quote: ... The nicked strand of DNA was then digested using Exonuclease (lU/ul, M0206, NEB) in NEB buffer l at 37 °C for lS min in a humidified chamber ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated using NEB Monarch RNA prep kit (New England BioLabs). The RNA concentration and RIN (RNA integrity number ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription was performed on 0.5µg DNase-treated total RNA using Lunascript RT (NEB) in 20µl reactions ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was isolated using Monarch RNA prep kit (NEB). Reverse transcription was performed on 0.5µg DNase-treated total RNA using Lunascript RT (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... using the Luna Universal qPCR Master Mix (NEB). Primers for qPCR are listed in the Key resource table.
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bioRxiv - Cell Biology 2024Quote: ... 500 ng gDNA was digested with 50 U PstI-HF (NEB) and 50 U ApoI-HF (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... for 20 min at 37 °C in RNAse H buffer (NEB). To image and segment whole l.2 Mb tracing loci ...
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bioRxiv - Cell Biology 2024Quote: ... a total of 600 ng of genomic DNA from the relevant cell lines was fragmented using DNA fragmentase (NEB, M0348) for 25 minutes at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... coli (NEB C3019) grown on LB Agar plates with 100ug/mL ampicillin ...
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bioRxiv - Cell Biology 2024Quote: ... Library fragment size and concentrations were quality verified by qPCR using the NEBNext Library Quant Kit (NEB, E7630) and loading 1:1 ...
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bioRxiv - Developmental Biology 2024Quote: ... plasmid templates were linearized with NotI and purified using Monarch PCR purification kits (New England Biolabs). The purified templates were transcribed using mMESSAGE mMACHINE Sp6 (Thermo-Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... mRNAs were purified using the Monarch RNA Cleanup Kit (New England BioLabs) and eluted in RNAse-free water ...
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bioRxiv - Cell Biology 2024Quote: ... according to the manufacturer’s instructions and then treated with Antarctic Phosphatase (New England Biolabs) and incubated at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: The cell lysates or purified PINK1 bound to FLAG beads were incubated at 30°C for 1h with 200 units of ʎ protein phosphatase (LPP) in a total reaction of 100µL following the instructions of the manufacturer (New England Biolabs #P0753).
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bioRxiv - Cell Biology 2024Quote: ... the HIRA-WT and HIRA-W799A-D800A coding sequences.59 using NEBuilder® HiFi DNA Assembly Master Mix (NEB) and verified all constructs by Sanger sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... The medium was then replaced with a fresh imaging medium containing 1 µM SNAP Cell Block (NEB, S9106S) to prevent the binding of residual SNAP dye to newly synthesized SNAP-tagged proteins ...
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bioRxiv - Cell Biology 2024Quote: ... Indexed cDNA libraries were obtained using the NEBNext Ultra II directional RNA Library Prep Kit for Illumina® E7760L (NEW ENGLAND BioLabs Inc) following manufacturer recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... and NEBNext Ultra TMII Directional RNA Library Prep Kit (New England Biolabs). Libraries were sequenced with an Illumina NovaSeq 6000 to obtain 150 bp ×2 paired-end reads ...
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bioRxiv - Cell Biology 2024Quote: ... AcGFP and TagRFP-657 proteins and SNAP-Surface 488 and Alexa 647 dyes (New England Biolabs) were measured in the same excitation and emission ranges as in flow cytometry assays ...
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bioRxiv - Cell Biology 2024Quote: ... infrared IR800-dye conjugated 3’ adapters were ligated overnight using RNA ligase 1 (NEB) in a high-percentage PEG8000 buffer ...
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bioRxiv - Cell Biology 2024Quote: ... and the RNAs were 3’end dephosphorylated by PNK (NEB) and FastAP phosphatases (Thermo) ...
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bioRxiv - Cell Biology 2024Quote: ... These cells that were pre-stained with 5 µM SNAP-Surface 647 (New England Biolabs) for one hour at room temperature if necessary were stained with 5µg/mL of Alexa488 labeled lectins (PNA ...
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bioRxiv - Cell Biology 2024Quote: ... 1mM Ribonucleoside vanadyl complex (RVC, New England Biolabs), and 40U/ml RNAsin plus (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... Gibson reactions were set up with NEBuilder HiFi DNA Assembly Master Mix (NEB E2621), 50ng of linearized backbone ...
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bioRxiv - Cell Biology 2024Quote: ... Exonuclease I (0.15 U/µl, New England Biolabs) and exonuclease III (0.77 U/µl ...
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bioRxiv - Cell Biology 2024Quote: ... Components of the insert were PCR amplified using Q5-Hot Start DNA Polymerase (NEB M0493) per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: EGFP was PCR-amplified from pCIneo-FlucDM-EGFP119 and subcloned in pcDNA4/TO using the restriction enzymes XhoI and XbaI (NEB). Nudfa1 and Ndufa2 was amplified from HEK293T cDNA and cloned into pcDNA4/TO-EGFP using KpnI and XhoI (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... and exonuclease III (0.77 U/µl, New England Biolabs) were then added with NEBuffer I to digest DNA with exposed end ...
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bioRxiv - Cell Biology 2024Quote: ... Nudfa1 and Ndufa2 was amplified from HEK293T cDNA and cloned into pcDNA4/TO-EGFP using KpnI and XhoI (NEB). Gene sequences of At-A1 ...
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bioRxiv - Cell Biology 2024Quote: ... the entry vectors pENTR2B-EPLINα and pENTR2B-EPLINβ were first generated using HiFi DNA assembly according to the manufacturer’s instructions (New England Biolabs).The primer sequences used to make the plasmids are shown below ...
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bioRxiv - Cell Biology 2024Quote: ... pBluescript II KS (+) and ORF were digested using SacI and SalI (New England Biolabs) restriction enzymes according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... using the SYBR green master mix (BioLabs, M3003E) using CFX Opus 384 Real-Time PCR System with One software (v2.2.2 ...
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bioRxiv - Cell Biology 2024Quote: ... cleaved with BsmBI (NEB). Final construct (pXPR_001-sgRAB21 ...
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bioRxiv - Cell Biology 2024Quote: ... the H3.1-SNAP and H3.3-SNAP were labeled with SNAP-Cell 505-Star (New England Biolabs, S9103S) for a duration of 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... the Monarch Genomic DNA Purification kit (New England Biolabs) was utilized with 1-2 x 106 cells ...