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Citations for New England Biolabs :
3651 - 3700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... 0.81 nmol LbCas12U protein and 0.45 nmol of each of three crRNAs were assembled in 1 x Nuclease Reaction Buffer (NEB). Protein and crRNAs were mixed and incubated for 10 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesised using the LunaScript kit (New England Biolabs). PCR amplification of WLV was done using PuReTag Ready-to-Go PCR beads (Illustra ...
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bioRxiv - Plant Biology 2024Quote: ... This fragment was inserted by assembly reaction (NEBuilder HiFi DNA Assembly Mix, New England Biolabs) between the CaMV 35S promoter and HDV ribozyme sequences of the small ...
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bioRxiv - Plant Biology 2024Quote: ... using Phusion polymerase (New England Biolabs) and cloned in the pENTR/D-TOPO vector ...
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bioRxiv - Plant Biology 2024Quote: ... the complete genome of WLV was amplified using Phusion DNA polymerase (New England Biolabs) from total RNA of WLV-infected Ch ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was done using the Luna Universal qPCR Master Mix (New England Biolabs) with WLV primers 4361+4264 and as the reference gene spinach Actin 11 (primers 4614+4615 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Media was aspirated and cells were resuspended in 50 uL Cell Lysis Buffer (10X) and 0.5 uL of Proteinase K (New England Biolabs P8107S) per 96-well ...
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bioRxiv - Synthetic Biology 2024Quote: ... and mCherry coding sequences at an AGGT site via Gibson assembly using Hifi DNA Assembly Master Mix (NEB, M5520) according to manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: All plasmids for transfection were constructed via BsaI (NEB, R3733L) Golden Gate cloning using “part vectors” corresponding to promoter ...
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bioRxiv - Synthetic Biology 2024Quote: ... Expression plasmids were assembled via BsaI (New England Biolabs) Golden Gate Assembly using pPVs or Q5 polymerase-amplified and gel-purified DNA fragments ...
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bioRxiv - Synthetic Biology 2024Quote: ... the plasmid is assembled with Gibson assembly protocol using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621S). Assembled DNA was transformed into competent cells ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli NEB10B (NEB, C3019I) grown in LB Broth Lennox (240230 ...
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bioRxiv - Synthetic Biology 2024Quote: ... New England Biolabs (NEB). Assembly of pDGB3alpha clones was performed using BsaI-HF and T4 Ligase (NEB).
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bioRxiv - Synthetic Biology 2024Quote: ... DNA was digested with SalI restriction enzyme (NEB) overnight ...
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bioRxiv - Synthetic Biology 2024Quote: ... Assembly of pDGB3alpha clones was performed using BsaI-HF and T4 Ligase (NEB).
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bioRxiv - Synthetic Biology 2024Quote: ... The library kit and type of sequencer were TruSeq Stranded Total RNA (NEB Microbe) and NovaSeq ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA fragments to be assembled were amplified by PCR using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532S). The PCR mix underwent gel electrophoresis for purification ...
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bioRxiv - Synthetic Biology 2024Quote: ... followed by purification with Monarch® PCR & DNA CleanupKit (NEB). Successful DNA assembly was verified first by colony PCR using GoTaq® Green Master Mix (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... This was incubated for 40 minutes at 65°C before 10µl of proteinase K (New England Biolabs; P8107S) was added and incubated for a further 20 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... 0.25% β-mercaptoethanol) and 30µl RNase A (New England Biolabs; T3018L). This was incubated for 40 minutes at 65°C before 10µl of proteinase K (New England Biolabs ...
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bioRxiv - Plant Biology 2024Quote: ... The protein samples were treated with Endo Hf (New England BioLabs, Ipswich, MA, USA) at 37°C for 1 h ...
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bioRxiv - Plant Biology 2024Quote: ... The NebNext Ultra II RNA library prep kit (New England Biolabs, MA, USA) was used to synthesize RNA libraries ...
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bioRxiv - Plant Biology 2024Quote: ... we generated libraries using NEB Ultra II Directional RNA with Poly(A) Selection (New England Biolabs, Ipswich, MA). Libraries were sequenced on an Illumina Novaseq generating 34.1 ± 13.7 million raw reads per sample.
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Physiology 2024Quote: ... We digested the PCR products with AgeI and NotI (New England Biolabs) and cloned them into the first position of the bicistronic vector pCAG-[first position]-2A-GFP ...
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bioRxiv - Physiology 2024Quote: ... and Q5 DNA polymerase were purchased from New England Biolabs (NEB). Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega ...
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bioRxiv - Physiology 2024Quote: ... mRNA sequencing libraries were prepared by OMRF with the xGen RNA Lib Prep Kit (Integrated DNA Technologies) and NEB poly-A selection kit (New England Biolabs). The mRNA libraries were then sequenced as 150 bp paired-end reads on an Illumna NovaSeq 6000 ...
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bioRxiv - Microbiology 2024Quote: ... Q5 high-fidelity Taq DNA polymerase (New England Biolabs Inc., Ipswich, MA) which has a 3’→ 5’ exonuclease activity and hence results in ultra-low error rates was used for PCR ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified with DNA Spin and Concentrate kit (Zymo Research, Irvine, CA, D4013 or NEB Monarch, T1030) following manufacturer instructions or gel-purified from kit (Zymo Research) ...
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bioRxiv - Microbiology 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (NEB; E6640S). Libraries were sequenced on Illumina NovaSeq 6000 2×150 with the UW-Madison Biotechnology Center at ∼10 million reads per sample.
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bioRxiv - Microbiology 2024Quote: ... DNA was digested with restriction enzymes from New England Biolabs (NEB). PCR products were purified with DNA Spin and Concentrate kit (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... rRNA-depleted samples were prepared for next-generation sequencing using NEBNext Ultra II Directional RNA Library Prep kit (NEB; E7765S) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Large (Klenow) Fragment (NEB) for blunting the fragment ...
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR products were ligated using a Gibson assembly kit (NEB, USA). The resulting plasmid pLZ asnA R100K was electroporated into strain S119 ΔasnA for expression of mutated asnA.
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bioRxiv - Microbiology 2024Quote: ... Input control and HT-enriched samples were prepared for Illumina sequencing using NEBNext Ultra II Library Prep Kit for Illumina (NEB; E7645S) following manufacturer’s protocol and sequenced on Illumina NovaSeq 6000 2×150 with the UW-Madison Biotechnology Center at ∼10 million reads per sample.
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bioRxiv - Genomics 2024Quote: ... 10% of these RNAs were decapped and polyphosphates reduced to monophosphates using RppH (NEB) to sequence all small RNAs (sRNA-seq).
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bioRxiv - Microbiology 2024Quote: ... and fragments were joined via Gibson Assembly (NEB).
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bioRxiv - Microbiology 2024Quote: ... gRNA oligos from IDT were cloned into Pss013 using Gibson Assembly (NEB). Plasmids were transformed into DH5α E ...
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bioRxiv - Genomics 2024Quote: ... 40 fmol of plasmid backbone and 2 μL of each annealed oligo pair were added to a one-pot golden gate reaction with Esp3I and T4 DNA Ligase (NEB) as has been done in previous studies73,75 ...
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Pre-implantation genome-wide methylation enables environmental adaptation in a social meso-carnivorebioRxiv - Genomics 2024Quote: ... then end-prepped and dA-tailed using the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, United Kingdom). Methylated adaptors were ligated to end-repaired and dA-tailed DNA using the NEBNext Ultra II Ligation Module (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... crRNAs were amplified from genomic DNA (primer sequences can be found in (Supp. Table 2) using Q5 2x Master Mix (NEB) in 100 μL reactions with the following thermal cycling parameters:
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bioRxiv - Genomics 2024Quote: ... cloning was performed using PCR of desired insert fragments with Q5 (New England Biolabs (NEB)) ...
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bioRxiv - Genetics 2024Quote: ... pLVX vector was first linearized by EcoR1 and BamH1 (NEB) and purified as the cloning backbone ...
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bioRxiv - Genomics 2024Quote: ... then phosphorylated using T4 Polynucleotide Kinase (NEB) in a 50 μL reaction ...
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bioRxiv - Genomics 2024Quote: ... while the PCR amplicon was digested with BsaI (NEB). These were purified using the DNA Clean and Concentrator Kit (Zymo) ...
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Pre-implantation genome-wide methylation enables environmental adaptation in a social meso-carnivorebioRxiv - Genomics 2024Quote: Genomic DNA was extracted and purified from 200 μL of whole blood using Monarch Genomic DNA Purification Kits (New England Biolabs, United Kingdom). DNA quantity and quality were assessed using NanoDrop 2000 (Thermo Scientific ...
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Pre-implantation genome-wide methylation enables environmental adaptation in a social meso-carnivorebioRxiv - Genomics 2024Quote: Samples of ∼200 ng genomic DNA were digested with 20 units of MspI (C↓CGG) and 10 units of AluI (AG↓CT) (New England Biolabs, United Kingdom) at 37°C overnight (∼16 hours) ...
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bioRxiv - Genetics 2024Quote: ... a test transformation was performed with 1uL of the 20uL SPRI purified Gibson assembly mixture and 50uL of 10-beta electrocompetent cells (NEB) to determine the optimal amount of Gibson assembly and 10-beta cells to achieve the desired colony-forming unit (CFU ...
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bioRxiv - Genetics 2024Quote: ... with primers (Integrated DNA Technologies, Coralville IA) designed to provide flanking homology arms for HiFi assembly (New England Biolabs, Ipswich MA) into EcoRI/NotI-digested (New England Biolabs ...