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Citations for New England Biolabs :
3551 - 3600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... including protein disulfide bond enhancer (NEB) and GamS (NEB) ...
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bioRxiv - Biochemistry 2024Quote: ... Ni-NTA elute was enriched on amylose resin (NEB, Cat. no: E8021L) and washed with 20mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... We expressed the His-SUGCT in Lemo cells (NEB), inoculating 4L of TB with 4 mL overnight culture ...
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bioRxiv - Biochemistry 2024Quote: ... Correct assembly was verified by PCR using Taq 2X Master Mix (New England BioLabs) and Sanger sequencing (Genewiz).
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bioRxiv - Biochemistry 2024Quote: ... using XhoI and NcoI (New England Biolabs) followed by T4 DNA ligase (New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... Restriction enzyme digest or Gibson Assembly (New England Biolabs, Ipswich, MA, USA) was used to clone each antibody sequence plus 400 base pair (bp ...
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bioRxiv - Bioengineering 2024Quote: Full length heavy and light chains for each antibody were cloned by restriction enzyme digest or Gibson Assembly (New England Biolabs) into pCMVR either individually or with a linker ...
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bioRxiv - Biochemistry 2024Quote: ... we used NotI and BlpI (NEB) to generate a 3841bp fragment ...
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bioRxiv - Biochemistry 2024Quote: The PLpro library was cut with NotI and NdeI (NEB) to release a 3908bp fragment containing PLpro and the barcode ...
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bioRxiv - Biochemistry 2024Quote: ... 30nM sgRNA and 30 nM Cas9 protein (NEB) for 1h at room temperature and analyzing cleavage products on a 2% agarose gel ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was mixed with the vector fragment and assembled with HiFi assembly (NEB). This strategy maintains variant barcode matching from residues for the library up until L290 ...
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bioRxiv - Biochemistry 2024Quote: ... All individual PLpro mutations were introduced into pDONR221-PLpro using NEB Q5 Site-Direct Mutagenesis Kit (NEB) following manufacturer’s instructions and shuttled into FU-tetO-Gateway-rtTA-2A-Puro using LR clonase.
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bioRxiv - Biochemistry 2024Quote: ... were generated by PCR using λ DNA template (NEB, N3011S)31 ...
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bioRxiv - Bioengineering 2024Quote: ... The positive sense RNAs were capped with vaccinal capping enzyme (NEB # M2080S) and methyltransferase (NEB# M0366S ...
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bioRxiv - Bioengineering 2024Quote: ... The total RNA was isolated at 24 hpi and decapped with mRNA decapping enzyme (NEB# M0608S) and ligated head to tail by using T4 RNA ligase 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Poly(A) RNA enrichment was conducted using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490), and the sequencing library was constructed using the NEBNext Ultra II RNA Library Prep Kit (E7765L ...
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bioRxiv - Bioengineering 2024Quote: ... and methyltransferase (NEB# M0366S) by following the vendor’s manuscripts.
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bioRxiv - Cancer Biology 2024Quote: ... DNAse type IV (New England Biolabs/ Bioconcept) in DMEM/F12 (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... M13mp18 circular DNA (P- 107, Bayou Biolabs) was used as the scaffold for the staples (IDT) ...
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bioRxiv - Biochemistry 2024Quote: ... followed by T4 DNA ligase (New England Biolabs) to generate a C-terminal His-tag preceded by a TEV protease cleavage site ...
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bioRxiv - Cancer Biology 2024Quote: ... 2ul mRNA decapping enzyme (New England Biolabs M0608S), and 1ul RNase inhibitor and incubating at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2024Quote: The ATP binding mutants (APLFD11A, APLFR37A, APLFK95A) were generated using Q5® Site- Directed Mutagenesis Kit (NEB #E0554) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and end-repair and ligation was performed using New England Biolabs (NEB) adapters ...
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bioRxiv - Biochemistry 2024Quote: ... Wildtype PLpro and variants were transformed into BL21/DE3 (NEB) and grown in Super Broth (WEHI Bioservices ...
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bioRxiv - Biochemistry 2024Quote: ... Three fragments were cloned in a single HiFi Assembly reaction (NEB). 1 ...
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bioRxiv - Biochemistry 2024Quote: ... The Assembly reactions were transformed into DH-10b electro-competent cell (NEB) and split into 2% and 98% before spreading on Agar plates containing 50ng/ul Kanamycin ...
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bioRxiv - Microbiology 2024Quote: ... Paired-end sequence libraries were prepared using the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc. ...
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bioRxiv - Microbiology 2024Quote: ... equal volumes of cDNA were used for qPCR using the Luna® Universal qPCR Master Mix kit (New England Biolabs). For qPCR ...
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GIBBERELLIN SIGNALING THROUGH RGA SUPPRESSES GCN5 EFFECT ON STAMEN ELONGATION OF ARABIDOPSIS FLOWERSbioRxiv - Plant Biology 2024Quote: ... Luna® Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA) and the ABI StepOnePlus™ system (Applied Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... thaliana cpSRP54 were cloned via GoldenGate (NEB) into a pET24d derivative (AL324 ...
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bioRxiv - Systems Biology 2024Quote: ... The PCR products were digested with DpnI (New England Biolabs) at 37°C for 1 hour to remove the original methylated template ...
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bioRxiv - Physiology 2024Quote: ... Nuclear proteins were extracted using nuclear extraction buffer (NEB) (20mM HEPES pH7.9 ...
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bioRxiv - Physiology 2024Quote: ... with 1x GlycoBuffer 1 (NEB), 5 μL of PNGaseF (500 U/μL ...
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bioRxiv - Physiology 2024Quote: ... 5 μL of O-glycosidase (40,000 U/μL, NEB) with 1x GlycoBuffer 3 (NEB) ...
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bioRxiv - Physiology 2024Quote: ... with 1x GlycoBuffer 1 (NEB). Following incubation ...
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bioRxiv - Systems Biology 2024Quote: ... Uracil-Specific Excision Reagent (NEB, USA) was used as previously described142.The reaction was run for 2h at 37°C and the resulting spatially barcoded cDNA libraries were collected and libraries prepared as described previously142 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of purified DNA template was used in the Hi-Scribe T7 transcription kit (NEB) at 37°C overnight (∼16 hr.) ...
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bioRxiv - Molecular Biology 2024Quote: ... attB plasmid containing genes for tdMCP-protein fusions and a MS2-circRNA barcode were digested overnight at 37°C to remove existing barcode sequence (2 μg plasmid, 5 μL 10x CutSmart buffer, 2 μL BsrGI-HF (NEB cat#R3575S), nuclease-free water to 50uL) ...
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bioRxiv - Molecular Biology 2024Quote: ... was digested by combining the following and incubating overnight at 37°C: 2 μg plasmid + 5 μL CutSmart buffer (10x) + 2 μL AflII (NEB cat# R0520S) + 2 μL BlpI (NEB cat# R0585S ...
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bioRxiv - Molecular Biology 2024Quote: ... and NheI-HF (NEB cat# R3131S) then submitted to University of Washington PacBio Sequencing Services for further processing ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 μL recombinant shrimp alkaline phosphatase (NEB cat#M0371S) was added and incubated at 37°C for 2 hrs ...
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bioRxiv - Systems Biology 2024Quote: ... 3 µL USER enzyme (NEB) were added to each sample and incubated at 37°C for 15 min and followed by a purification with 50 µL AMPure XP beads according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 1x protease inhibitors) and incubated with 0.3 U/μl CIP (New England BioLabs) for 1 hour at room temperature prior to loading onto a gel.
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bioRxiv - Molecular Biology 2024Quote: ... coli (NEB cat#C2987H) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... All reactions were mixed to 22 µl including 10 U of HindIII-HF (NEB, Catalog #R3104L) and up to 2 µl of QuickExtract lysates ...
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bioRxiv - Synthetic Biology 2024Quote: ... Restriction enzymes were obtained from NEB, except for DNase I (Roche 4716728001) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Oligos were phosphorylated using T4 polynucleotide kinase (T4 PNK, NEB M0201S) in a reaction containing 1 µl of 100µM forward oligo ...
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bioRxiv - Synthetic Biology 2024Quote: ... purchased from NEB, was served as the backbone for the all sgRNA delivery plasmids ...
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bioRxiv - Zoology 2024Quote: ... The annealed dsRNAs (2.5 µg) were checked on 1.5% agarose gel by running them together with 2 µL of dsRNA ladder (NEB# N0363S, Germany) (Fig ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.0005 U/µL RNase H (NEB #M0297L), and 2.5 U/µL M-MuLV RT (NEB #M0253L ...