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Citations for New England Biolabs :
3501 - 3550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The sequence of the rhamnose construct starting at the ilvBN terminator and ending with the rhaBAD cassette of pCK355 [66] was amplified using Q5 polymerase (NEB), adding overlaps for AQUA cloning into pUC19 [67] ...
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bioRxiv - Microbiology 2024Quote: ... in vitro translation of the short-model mRNA was performed using a PURExpress® In VitroProtein Synthesis Kit (New England Biolabs, US). The reaction mixture (volume ...
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bioRxiv - Microbiology 2024Quote: ... Genomic libraries were prepared using the NEBNext Ultra II FS library preparation kit (New England Biolabs, USA) according to the manufacturer’s instructions for MiSeq (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... (NEB). The p57mA3 plasmid was digested with MscI restriction enzyme and the following oligomers were used ...
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bioRxiv - Microbiology 2024Quote: The msdDNA cDNA was isolated from acrylamide gels and 100 ng was used to extend the 3’ end with dCTP or dGTP and terminal deoxynucleotidyl transferase (TdT) from NEB, according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Spacers were introduced into pNA15 via Golden Gate cloning with BsaI-HFv2 (New England Biolabs Cat. #R3733S) and T7 DNA ligase (New England Biolabs Cat ...
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bioRxiv - Microbiology 2024Quote: ... 200 ng of nucleic acids from each complex were mixed in 1xDNase I buffer (NEB) with either RNase-free DNase I (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... and T7 DNA ligase (New England Biolabs Cat. #M0318S), then electroporated into Endura Duo electrocompetent cells (Biosearch Technologies Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... the lentiCRISPR-v2 vector underwent BsmBI-v2 (NEB, Cat# R0739L) digestion and Quick CIP (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... digestion and Quick CIP (NEB, Cat# M0525S) dephosphorylation ...
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bioRxiv - Microbiology 2024Quote: ... the pUC19-PRha-YFP-3×FLAG plasmid was inverse amplified by PCR (Q5 polymerase, NEB), omitting the yfp gene ...
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bioRxiv - Microbiology 2024Quote: ... Poly(A) mRNA was isolated via NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) or the Ribo-Zero rRNA removal Kit (Illumina) ...
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bioRxiv - Microbiology 2024Quote: ... Then we used T7 Ribomax Express (NEB) in vitro RNA synthesis kit to generate single-stranded negative sense RNA that resembles the vRNA molecule ...
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bioRxiv - Microbiology 2024Quote: ... were prepared by cloning the codon-optimized (E.coli expression) synthetic gene fragments into pMAL-p2x vector (New England Biolabs) for periplasmic expression in BL21-CodonPlus(DE3)-RIPL (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The Monarch RNA Cleanup Kit (NEB) was used to purify the resulting RNA ...
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bioRxiv - Microbiology 2024Quote: ... and detected using anti-MBP antibodies (New England Biolabs). As a negative control ...
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bioRxiv - Microbiology 2024Quote: ... Using Gibson Assembly (New England Biolabs), the upstream and downstream regions of ttdV ...
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bioRxiv - Neuroscience 2024Quote: ... 350 ng of total RNA were used for mRNA enrichment using NEBNext mRNA polyA isolation module (NEB, Ornat, Rehovot, Israel, #E7490L) and libraries for Illumina sequencing were prepared using the NEBNext Ultra II RNA kit (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... and libraries for Illumina sequencing were prepared using the NEBNext Ultra II RNA kit (NEB, Ornat, Rehovot, Israel, #E7770L). Quantification of the library was performed using dsDNA HS assay kit and Qubit (Molecular Probes ...
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bioRxiv - Neuroscience 2024Quote: ... 20μL of DNA were used for libraries preperation using the NEBNext Ultra II DNA kit (NEB, #E7645). Quantification of the library was performed using dsDNA HS Assay Kit and Qubit 2.0 (Molecular Probes ...
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bioRxiv - Systems Biology 2024Quote: ... we directly engineered the pre-barcoded pSL51 and pre-barcoded pSL737 plasmid pools using PCR and HiFi in vitro recombinational assembly (NEB reagents) to generate a construct that should express a single methionine start-codon fused to the Gateway attL2/attR2 “scar” sequence (YPAFLYKVV) ...
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bioRxiv - Systems Biology 2024Quote: ... was electroporated into 10beta electrocompetent cells (NEB). After recovery ...
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bioRxiv - Systems Biology 2024Quote: ... electroporated into 10beta electrocompetent cells (NEB), then the 1-hour-recovered cells were plated to select on LB+Carb plates ...
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bioRxiv - Zoology 2024Quote: ... For fresh specimens a shearing step with NEBNext dsDNA Fragmentase (New England Biolabs) was performed before library preparation ...
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bioRxiv - Systems Biology 2024Quote: Each tagged-ORF plasmid subpool was treated with I-SceI (NEB) in the provided buffer (at a volume of 50uL for ∼1ug hORF plasmids and 200uL for ∼4ug vORF plasmids) ...
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bioRxiv - Systems Biology 2024Quote: Approximately 6ng of each loxcode cassette was combined with approximately 75ng of the linear digested plasmid pool in a final 10uL of 1x HiFi assembly reaction (NEB). This was incubated on a thermocycler at 50C for 15min ...
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bioRxiv - Microbiology 2024Quote: ... and Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... the AT1G65620/AS2 coding sequence (600 bps) was amplified from Col-0 cDNA using primers oLH111 and oLH112 by Phusion PCR (NEB), and cloned into pENTR™/D-TOPO™ (Invitrogen™) ...
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bioRxiv - Systems Biology 2024Quote: The dsDNA loxcode cassettes were formed by combining two purchased oligos (IDT) in a 50uL Q5 (NEB) polymerase reaction with the Q5 polymerase ...
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bioRxiv - Systems Biology 2024Quote: ... This was cooled and treated with phosphatase rSAP (NEB) at 37C for 30min ...
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bioRxiv - Systems Biology 2024Quote: ... The poly(A) mRNA-enriched libraries were constructed using NEBNext® Ultra™ II RNA Library Prep Kit (New England BioLabs, Ipswich, MA). Paired-end sequencing (2×150 bp ...
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bioRxiv - Systems Biology 2024Quote: ... Ring-ligation was carried out using T4 DNA ligase (NEB) at 16 °C overnight ...
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bioRxiv - Systems Biology 2024Quote: ... Destination vectors were linearized using single-cutter restriction enzymes (New England Biolabs R0520L ...
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bioRxiv - Systems Biology 2024Quote: ... followed by purification using a Monarch PCR&DNA Cleanup Kit (NEB). Ring-ligation was carried out using T4 DNA ligase (NEB ...
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bioRxiv - Systems Biology 2024Quote: ... The samples then underwent two separate digestion reactions (with up to 4 µg of genomic DNA) using NlaIII and MseI enzymes (NEB) at 37°C overnight ...
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bioRxiv - Systems Biology 2024Quote: ... 1000 bp directly upstream and downstream of the HIS4-targeting sgRNA on the genome was PCR amplified and cloned on the upstream and downstream of the pENO1-Cas9-PptefT on D-227 vector using New England BioLabs (NEB) NEBuilder® HiFi DNA Assembly Master Mix ...
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared by the West Virginia University Genomics Core using the NEBNext Low Input/Single Cell RNA-Seq kit (New England Biolabs). Libraries were sequenced to 30 million reads on a Nextseq 2000 (Illumina ...
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bioRxiv - Immunology 2024Quote: ... with the exception of KLF2 which was performed using the Q5 High-Fidelity kit (NEB). For the 11q deletion variant (P3) ...
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bioRxiv - Immunology 2024Quote: Streptavidin magnetic beads (BioLabs, Cat. #S1420S) were washed twice with PBST and incubated with recombinant biotinylated spike proteins (spike ...
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bioRxiv - Microbiology 2024Quote: ... 5 μL of GC Enhancer (NEB), 5 μL of 5X buffer ...
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bioRxiv - Microbiology 2024Quote: ... into the HindIII and SpeI restriction sites of the pSEVA23a1 vector79 where a hygromycin resistance cassette had been inserted in BamHI and XbaI (all restriction enzymes were from New England Biolabs). Correct cloning was confirmed by Sanger sequencing (Eurofins) ...
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bioRxiv - Cell Biology 2024Quote: ... 15 µl of genomic DNA (DNA concentration is around 100 ng/µl) were digested or mock with 20 units of BsrGI or BamHI restriction enzymes (New England BioLabs) for 5 hours at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... The region of interest was then amplified using Q5 2× mastermix (New England Biolabs, cat. M0492S), 500 ng of template DNA ...
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bioRxiv - Immunology 2024Quote: Poly A-enriched libraries were made using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, cat. E7770) and sequenced on a NovaSeq SP with PE150 read length.
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bioRxiv - Immunology 2024Quote: Sequencing: Poly A-enriched libraries were made using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, cat. E7770) and sequenced on a NovaSeq SP with PE150 read length.
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bioRxiv - Molecular Biology 2024Quote: ... vanadyl ribonucleoside complex (VRC; New England Biolabs) was added to the NPB at a concentration of 10 mM ...
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bioRxiv - Microbiology 2024Quote: ... Bisulfite-converted RNAs were then end-repaired and subjected to NEBNExt Small RNA Library Prep Set for Illumina (NEB, E7330L), according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... 20-50 ng of purified RNA was processed by NEBNext rRNA Depletion Kit (New England BioLabs, Inc.) for removing ribosomal RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... LongAmp® Hot Start (New England Biolabs, USA); Phusion™ Hot Start II DNA Polymerase (Invitrogen™ ...
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bioRxiv - Molecular Biology 2024Quote: ... Q5U® Hot Start High-Fidelity DNA Polymerase (New England Biolabs, USA); NEB Next® Ultra™ II Q5® mastermix (New England Biolabs ...