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Citations for New England Biolabs :
3451 - 3500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... and the excess of FTSC was removed with Monarch RNA Cleanup Kit (NEB).
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bioRxiv - Biophysics 2024Quote: ... The purified dsDNA templates were transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... and the resulting RNAs were isolated from the reaction mixtures by Monarch RNA Cleanup Kit (NEB). The size and purity of the RNA constructs was confirmed by TBE-urea PAGE (Supplementary Fig ...
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bioRxiv - Biophysics 2024Quote: ... coli SHuffle® T7 (New England Biolabs) as described (63) ...
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bioRxiv - Genomics 2024Quote: ... A 7:1 molar ratio of barcode oligonucleotide to sublibrary was ligated overnight at 16°C with T4 DNA ligase (New England Biolabs).
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bioRxiv - Genomics 2024Quote: ... 25 μL Q5 polymerase (New England Biolabs), and 19 μL water ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were purified with enzymes ExoI and rSAP (New England Biolabs, Ipswich, MA, USA), Sanger sequenced (Macrogen ...
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bioRxiv - Genomics 2024Quote: ... PCR products were then digested at 37°C with DpnI (New England Biolabs) for 2 hours to remove residual starting plasmid ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of each sublibrary plasmid was digested at 37°C for 5 hours with NheI-HF and SacI-HF (New England Biolabs), incubated with rSAP for 30 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... End repair and dA-tailing were performed using the NEBNext End repair/dA-tailing Module (E7546, NEB). Ligation was then assessed with the Ligation Sequencing Kit 1D ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Three micrograms of DNA were repaired using the NEBNext FFPE DNA Repair Mix (NEB M6630). End repair and dA-tailing were performed using the NEBNext End repair/dA-tailing Module (E7546 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 50-250 ng of total RNA were used for library production with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB, #E7490), in conjunction with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Genomics 2024Quote: ... coli (New England Biolabs).
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bioRxiv - Bioengineering 2024Quote: ... All gene fragment amplifications were done using Seither Q5-polymerase (New England Biolabs; M0492L) or Superfi-II polymerase (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... and XhoI (New England Biolabs), gel-purified using Zymoclean Gel DNA Recovery kit (Zymo Research) ...
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bioRxiv - Genetics 2024Quote: ... were double digested with NheI-HF (New England Biolabs) and XhoI (New England Biolabs) ...
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bioRxiv - Genetics 2024Quote: ... and 1× Q5 buffer (New England Biolabs). Thermal cycling was performed as follows ...
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bioRxiv - Biophysics 2024Quote: ... First the 5’ triphosphate of RNA was converted into a 5’ monophosphate by incubating 100 µg RNA with 100 units of RNA 5’ Pyrophosphohydrolase (NEB, Ipswich MA) at 37°C for 1 hour within a 100 µl reaction volume ...
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bioRxiv - Genetics 2024Quote: Libraries for whole genome sequencing (WGS) were prepared according to the previously described scMDA protocol using a NEBNext Ultra II FS kit (NEB). For quality control purposes ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples collected from culture media were treated with PNGase F enzyme (New England Biolabs, P0709S) to remove the N-linked oligosaccharides from the TECTA protein according to the manufacturer protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... and FseI/AscI for CORIN and CTSK using T4 ligase (NEB, M0202).
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bioRxiv - Developmental Biology 2024Quote: ... The dephosphorylation reaction was supplemented with 0.5 μl T4 PNK (NEB), and the reaction was incubated for 25 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 500 ng cDNA was utilized as a template for amplification of RLN1 and RLN2 using Hot Start Taq 2X Master Mix (New England Biolabs) and GeneAmp PCR System 2700 thermal cycler (Applied Biosystems) ...
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bioRxiv - Genomics 2024Quote: ... and the top two scoring sgRNAs for each target gene based on depletion or enrichment phenotypes were cloned into dual sgRNA lentivirus expression vectors with direct capture tags (Addgene 187241)40 using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs #E2621L) (Table S2) ...
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bioRxiv - Biochemistry 2024Quote: All plasmids were constructed using either NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) for Gibson assembly28 ...
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bioRxiv - Biochemistry 2024Quote: ... using a Q5 Site-Directed Mutagenesis Kit (New England Biolabs), or by nicking mutagenesis32,60 ...
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bioRxiv - Genomics 2024Quote: ... and the top 3 sgRNAs were individually cloned into single sgRNA expression vectors with direct capture cs1 sequences (Addgene # 122238)10 using restriction digest (BstXI and BlpI) and T4 ligation (NEB # M0202M). A non-targeting sgRNA sequence was included as well ...
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bioRxiv - Biochemistry 2024Quote: ... then DpnI digest (New England Biolabs, 101228-926). The linear PCR product was then re-circularized with In-Fusion Cloning Master Mix (Takara Bio ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µM RNCs or empty 70S ribosomes (NEB) were incubated (30 min ...
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bioRxiv - Genomics 2024Quote: ... to the PCR-amplified pDR111 (using P3 and P4) using the HiFi DNA assembly protocol (New England Biolabs, USA). This resulted in the cloning of the mutL* polC* synthetic operon under the control of Phs into a B ...
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bioRxiv - Genomics 2024Quote: ... Prior to library preparation a UDG-treatment was performed using 16.25ul of purified DNA and 5 μl (1U/1uL) USER enzyme (New England BioLabs®, Inc.) and an incubation of 3 hours at 37°C ...
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bioRxiv - Genetics 2024Quote: ... PCR amplification was performed with Q5 High-Fidelity DNA Polymerase (NEB M0491S). Following DPN1 digestion ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of PCR product from the inner primer amplification was cleaned using 5μL Exonuclease I (NEB) and 2μL rSAP (NEB) ...
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bioRxiv - Bioengineering 2024Quote: ... and 2μL rSAP (NEB), incubating for 15 min at 37°C then 15 min at 80°C ...
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bioRxiv - Bioengineering 2024Quote: ... 2) parts were either synthesized as fragments (Twist Bioscience) and subsequently PCRed using Q5 (NEB), or synthesized as duplex oligos (IDT) ...
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bioRxiv - Genetics 2024Quote: ... RNA-seq library was prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs) and NEBNext® Ultra™ ll Directional RNA Library Prep Kit (New England BioLabs ...
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bioRxiv - Genetics 2024Quote: ... the library was prepared using the NEBNext DNA Library Prep Kit (New England BioLabs). The constructed library was sequenced on Novaseq 6000 platform (illumina ...
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bioRxiv - Microbiology 2024Quote: ... 25 ng of linearised plasmids were ligated with 75 ng of purified VH and VL by Gibson Assembly (New England BioLabs). The reaction was performed in a final volume of 5 μL ...
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bioRxiv - Microbiology 2024Quote: ... Specific probes were generated by end-labeling of oligodeoxyribonucleotides (Table S3) using T4 Polynucleotide Kinase (New England Biolabs) and [γ-32P]-ATP (Hartmann Analytic) ...
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bioRxiv - Microbiology 2024Quote: ... TAP-PCR was performed in a total volume of 25 μL containing 0.25 μL of Q5 polymerase (NEB), 5 μL of GC Enhancer (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... the upstream homologous region was cloned into the ApaI/KpnI-HF (NEB) digested pSFS2 plasmid ...
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bioRxiv - Microbiology 2024Quote: ... digested pSFS2 plasmid using NEBuilder HiFi (NEB) as per manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... 5-10 µg of DNase-treated RNA samples were denatured for 3 min at 70°C and separated on a 15% (w/v) TBE-urea gel (Novex®) with a low range ssRNA ladder (New England Biolabs, USA). The gel was stained with 1x SYBR gold dye (Thermo Fisher Scientific GmbH ...
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bioRxiv - Microbiology 2024Quote: ... the upstream homologous region was cloned into the XhoI/KpnI-HF (NEB) digested pSFS2 plasmid using NEBuilder HiFi (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Metagenomic sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep kit (New England Biolabs, MA, USA) with a targeted insert size of 350-400bp and Dual Index multiplex oligos ...
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bioRxiv - Microbiology 2024Quote: ... the sequence encoding amino acid residues 1 to 35 of the N-terminal end of BVG96_RS17270 (89) was PCR amplified using Q5 polymerase (NEB) and cloned via NEBuilder HiFi DNA Assembly (NEB ...
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bioRxiv - Microbiology 2024Quote: Molecular biology reagents were from NEB and chemical reagents were from Sigma unless otherwise noted ...
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bioRxiv - Microbiology 2024Quote: ... and cloned via NEBuilder HiFi DNA Assembly (NEB) into plasmid pIDMv5K-J231000-Dasher-GFP-B1006 (unpublished ...
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bioRxiv - Immunology 2024Quote: ... Immunoglobulin heavy and light chain cDNA was amplified using the Q5 High-Fidelity 2X Master mix (NEB) and pooled forward primers that bind the leader sequences of the variable regions and the reverse primers that bind the first exon of the mu and kappa constant regions ...
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bioRxiv - Microbiology 2024Quote: ... colony PCRs (GOTAQ, NEB) were performed on positive E ...