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Citations for New England Biolabs :
3351 - 3400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 4 µl of thermolabile proteinase K (New England Biolabs, P811S) added to Mitochondria-bound beads resuspended in 30 µl of KPBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Whole exome library construction was generated using the NEB Next® Ultra RNA Library Prep Kit for Illumina (E7530L, NEB, USA) after DNA shearing ...
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... the sgRNA regions were amplified from the genomic DNA by PCR using the NEBNext Q5 HotStart HiFi polymerase (NEB, M0543) and specific primers ...
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bioRxiv - Cancer Biology 2024Quote: ... the NEB Next® Ultra RNA Library Prep Kit for Illumina (E7530L, NEB, USA) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... T4 DNA Ligase (New England BioLabs #M0202L) and Ligase Buffer (New England BioLabs #B0202S) ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR reactions were prepared using the Luna® Universal qPCR Master Mix (NEB). Reactions were run and data analyzed using the QuantStudioTM 7 Flex Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... and T4 polynucleotide kinase (T4 PNK, NEB, M0201) was used to add a phosphate group at the 5′ ends ...
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bioRxiv - Cancer Biology 2024Quote: ... using T4 quick ligase (NEB, M2200), of sequencing adapters ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1.5ul of T4 Polynucleotide Kinase (NEB, M0201L). Samples were incubated at 37°C for 1h and then inactivated by addition of EDTA at a final concentration of 20mM ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA Fragments were repaired by T4 DNA polymerase (NEB, M0203), and T4 polynucleotide kinase (T4 PNK ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.5ul of Klenow Fragment (3’→5′ exo-, NEB, M0212S) and 1.5ul of T4 Polynucleotide Kinase (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... An adenosine base was then added by Klenow fragments (NEB, M0212) to allow efficient ligation ...
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bioRxiv - Cancer Biology 2024Quote: ... Nucleosomes labeling the following reaction was used: NEBuffer™ 2 (NEB B7202), inhibitors (as detailed above ...
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bioRxiv - Cancer Biology 2024Quote: ... Amplicons were generated with the Q5 2x mastermix (New England Biolabs) and cloned by GoldenGate assembly into pCFD5w (Addgene 112645 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 ng of purified CUT&RUN enriched DNA was used to prepare Illumina library using the NEBNext Ultra II DNA Library Prep kit (NEB, #E76450) per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... the pooled oligo library was amplified using NEB Next High-Fidelity PCR Master Mix (New England Biolabs, cat#M0541S). The PCR product was digested with Esp3I (BsmBI ...
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bioRxiv - Cell Biology 2024Quote: ... The vector was dephosphorylated using the rSAP (M0371S - NEB), loaded on a gel and the correct band was purified using Wizard SV Gel and PCR Clean-Up System ...
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bioRxiv - Cell Biology 2024Quote: ... followed by NEBNext Ultra II Direction Prep Kit for Illumina (NEB Cat #). Library sizes was measured using Bioanalyzer and pooled libraries concentration was calculated using NEBNext Lib Quant Kit (NEB Cat#) ...
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bioRxiv - Cell Biology 2024Quote: ... Library sizes was measured using Bioanalyzer and pooled libraries concentration was calculated using NEBNext Lib Quant Kit (NEB Cat#). Libraries were pooled and sequenced on an Illumina NextSeq 550 using paired-end sequencing of 42 bases per read.
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bioRxiv - Cell Biology 2024Quote: RNA-sequencing libraries were prepared NEBNext Poly(A) mRNA Magnetic Isolation Module kit (NEB Cat #) followed by NEBNext Ultra II Direction Prep Kit for Illumina (NEB Cat #) ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.5) supplemented with 1 U FURIN (#P8077S, NEB) for 6 h at 25°C ...
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bioRxiv - Cell Biology 2024Quote: ... MBP5-FN-paramyosin-ΔSal (#E8052S, NEB) containing a FURIN site ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was reverse transcribed with Moloney murine leukemia virus (M-MuLV) transcriptase (New England Biolabs). The purity and concentration of RNA were measured using NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... ATAC-seq libraries were prepared using the NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541) as previously described76 ...
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bioRxiv - Cancer Biology 2024Quote: ... 28 novel RNF43 RING domain variants were generated using Q5 mutagenesis (New England Biolabs). All ZNRF3 and RNF43 expression plasmids were full-length sequence verified ...
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bioRxiv - Cancer Biology 2024Quote: ... The first one made use of Q5 mutagenesis following the manufacturer’s instructions (New England Biolabs), but due to the high GC-content of exon1 specific for the ZNRF3 long isoform ...
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bioRxiv - Cancer Biology 2024Quote: ... Then the ORF of the ZNRF3 short variant replaced RNF43 and was assembled into the C-terminal FLAG-tagged vector using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and the GeneArt Gibson Assembly HiFi Master Mix (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... The 3xFLAG- and HA-tagged ZNRF3 expression vectors were generated with the Q5 Hot Start High-Fidelity DNA Polymerase Kit (New England Biolabs) by replacing the FLAG-tag ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using the Q5 High-Fidelity DNA Polymerase Kit (NEB) and primers specific for the microsatellite expansion adapters (1X Q5 Reaction Buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Forward and reverse primers (100 µM) were phosphorylated and annealed using T4 Polynucleotide Kinase (PNK; New England Biolabs M0201S) and 10x T4 Ligation Buffer (Thermo B69) ...
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bioRxiv - Cancer Biology 2024Quote: ... before ligation to microsatellite expansion adapters containing an EcoRV restriction site (listed in Supplemental Table 1) using the T4 DNA Ligase Kit (NEB) at a 5:1 ratio overnight at 16°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Adapter sequences were then removed by restriction digest (PCR reaction product, 1X rCutSmart NEB Buffer, 5 U EcoRV-HF (NEB)) at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Adapter sequences were then removed by restriction digest (PCR reaction product, 1X rCutSmart NEB Buffer, 5 U EcoRV-HF (NEB)) at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was amplified by PCR using the Q5 High-Fidelity DNA polymerase kit (New England BioLabs) following the manufacturer’s recommendations and with primers designed around the mutations (supplementary Table S9) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR reactions contained 2 μl cDNA in 50 μl PCR reaction with Phusion Hot Start Flex (New England Biolabs). Reaction conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using NEB UltraII RNA library Prep kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... For PCRs we used Phusion High-Fiedlity PCR Master Mix with HF Buffer (NEB) and reactions were run as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... To ensure simultaneous amplification of both the large unedited ( 12.6 kb) and edited allele (646 bp) we used the LongAmp Taq DNA Polymerase (NEB) which is specially formulated to amplify large products ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.1μL HaeIII (NEB – R0108S), 1 μL patient sample gDNA with a concentration range of 10 – 66 ng/μL and 6.7 μL nuclease free water to make a 20 μL total reaction ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting PCR products then underwent end-repair and A-tail addition followed by New England Biolabs (NEB) adapter ligation ...
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bioRxiv - Cancer Biology 2024Quote: ... These gene fragments were cloned into pcDNA3.1-PsChR2-eYFP backbone vector using the HiFi Assembly Kit (New England Biolabs, lpswich, MA).
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bioRxiv - Cancer Biology 2024Quote: ... linearized with BsmBI (NEB, #R0739S) and a PCR based insert produced by Phusion DNA Polymerase (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... by In Fusion cloning into pDual_dsCas9_Venus digested with BamHI-HF (NEB, #R3136). Dual-sgRNAs against chromosome 1 non-coding locus ...
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bioRxiv - Cancer Biology 2024Quote: ... and a PCR based insert produced by Phusion DNA Polymerase (NEB, #M0530L) containing a second sgRNA cloning site (SbfI) ...
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bioRxiv - Cancer Biology 2024Quote: ... EcoRI (NEB, USA) and BsmI (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... after which the cells were washed twice with DPBS and total RNA was extracted using the Monarch® Total RNA Miniprep Kit (NEB, Ipswich, MA). Total RNA was stored at −80°C until sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were immediately purified using a Qiagen minElute column and subjected to PCR amplification with NEBNext High-Fidelity 2X PCR Master Mix (NEB). Optimal PCR cycles were determined via qPCR to avoid over-amplification ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were then isolated using the Monarch® Plasmid Miniprep Kit (New England Biolabs, Ipswich, MA, USA) and used to transfect HEK293T cells (ECACC ...
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bioRxiv - Cancer Biology 2024Quote: ... Poly(A) mRNA was first separated using magnetic beads (NEB) and subsequently converted to cDNA ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA-sequencing libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep kit for Illumina® (NEB) following supplier’s recommendations ...