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Citations for New England Biolabs :
3401 - 3450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and BsmI (NEB, USA) were used to cut the sequence ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepped with the NEBNext Ultra II Directional RNA library Prep with Poly(A) isolation (New England Biolabs, Ipswich, MA). Reads were aligned to the mouse genome using Elysium ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was then extracted using the Monarch gDNA Purification Kit (NEB T3010S) following the standard protocol but with elution using 50 µL 25 mM K3BO3 at pH 7.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were treated with 3ul T4 PNK (New England Biolabs M0201), 1x PNK buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were prepared using the NEBNext small RNA library prep kit (New England Biolabs E7330) and sequenced by Novaseq PE150 ...
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bioRxiv - Cancer Biology 2024Quote: Libraries were prepared on beads using the NEBNext Ultra II DNA Library Prep Kit (NEB, #E7645). First ...
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bioRxiv - Cancer Biology 2024Quote: ... and T4 polynucleotide kinase (New England BioLabs (NEB)) ...
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bioRxiv - Cancer Biology 2024Quote: ... and T4 polynucleotide kinase (New England BioLabs (NEB)) ...
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bioRxiv - Cancer Biology 2024Quote: ... A single adenine base was added to fragment ends by Klenow fragment (3′ to 5′ exo minus; NEB), followed by ligation of Illumina adaptors (Quick ligase ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by ligation of Illumina adaptors (Quick ligase, NEB). The adaptor-ligated DNA fragments were enriched by PCR using the Illumina Barcode primers and Phusion DNA polymerase (NEB) ...
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bioRxiv - Cancer Biology 2024Quote: ... The adaptor-ligated DNA fragments were enriched by PCR using the Illumina Barcode primers and Phusion DNA polymerase (NEB). PCR products were size-selected using 3% NuSieve agarose gels (Lonza ...
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bioRxiv - Cancer Biology 2024Quote: ... beads and library preparation was carried out with the Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). The Illumina NovaSeq6000 platform was used for library sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... RNA sequencing library preparation used the NEBNext Ultra RNA Library Prep Kit for Illumina followed by manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... Protein A magnetic beads (New England Biolabs) were saturated with rabbit-anti-GFP ...
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bioRxiv - Cell Biology 2024Quote: ... and inserted into BamHI-HindIII-digested p413-PGPD vector using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, E5520S).
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bioRxiv - Cell Biology 2024Quote: ... pSNAPf vector containing SNAP-tag was purchased from NEB (N9183S) and optimised to Drosophila codon suing Genewiz (USA ...
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bioRxiv - Cell Biology 2024Quote: ... These fragments were assembled using a NEBuilder® HiFi DNA Assembly kit (NEB) to create a pET24a-anti-ALFA-std expression vector ...
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bioRxiv - Cell Biology 2024Quote: ... The amplified fragment containing SNAP was assembled with an amplified pUbq destination backbone using a NEBuilder® HiFi DNA Assembly kit (NEB) to create a pUbq-SNAP destination vector ...
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bioRxiv - Cell Biology 2024Quote: ... The pRNA destination backbone was linearised by primers s5 and s6 (Table 3) and assembled with the mScarlet-I3 fragment using a NEBuilder® HiFi DNA Assembly kit (NEB) to create a pRNA-mScarlet-I3 destination vector ...
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bioRxiv - Cell Biology 2024Quote: ... the constructs were digested and linearised by AscI (R0558S, NEB), precipitated with 10 mM sodium acetate and 7 mM EDTA in 66% ethanol overnight at −20 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Synthetic oligonucleotides encoding 3xFLAG were then ligated to the 5’ position of APEX2 sequence using NEBuilder HiFi DNA assembly kit (New England Biolabs) to form the final vector pcDNA5/FRT/TO/3xFLAG-APEX2.
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bioRxiv - Biophysics 2024Quote: ... coli NiCo21(DE3) strain (NEB). Cells were grown at 37°C supplemented with 2XYT medium (Melford ...
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bioRxiv - Biophysics 2024Quote: Cloning procedures involving PCR were performed using Q5 High-fidelity polymerase (New England Biolabs) according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... using the NEBuilder HiFi DNA Assembly kit (New England Biolabs); by strictly following the protocols recommended by the manufacturers ...
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bioRxiv - Biophysics 2024Quote: ... Gi was incubated in 0.02% DDM/CHS on ice for 30 minutes before addition of 0.5 µl apyrase (NEB) and ScFV at a 1:1 ratio (the final concentrations of Gi and ScFV were both ∼90 µM ...
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bioRxiv - Biophysics 2024Quote: ... A 4°C AKTA Pure FPLC system (Cytiva) was prepared with a 10 mL amylose column (NEB #E8021S) and 2 mL/min flow rate ...
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bioRxiv - Biophysics 2024Quote: ... Then the suspension was incubated at room temperature for 1.5 h after adding apyrase (25 mU/mL; New England Biolabs). The supernatant was removed by ultracentrifugation at 40,000 rpm for 30 min ...
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bioRxiv - Biophysics 2024Quote: ... Escherichia coli BL21 (DE3) competent cells (New England Biolabs) were transformed with a pET28a vector containing the N-terminal six-histidine tagged human TG2 ...
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bioRxiv - Biophysics 2024Quote: ... The resulting construct was expressed in Escherichia coli BL21(DE3) cells (New England Biolabs, C2527H) at 18 °C for 16 hours after induction with 0.2 mM isopropyl-β-D-thiogalactoside (Goldbio ...
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bioRxiv - Biophysics 2024Quote: ... and R88 using the Q5 site-directed mutagenesis kit (NEB). Giant spheroplasts were generated as described previously (Martinac et al. ...
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bioRxiv - Biophysics 2024Quote: ... The plasmid was linearized with NheI-HF (New England Biolabs) and purified using QIAquick PCR Purification Kit (QIAGEN) ...
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bioRxiv - Biophysics 2024Quote: ... 25 mU/mL Apyrase (New England Biolabs), and 100 μM TCEP at room temperature for 2 h ...
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bioRxiv - Biophysics 2024Quote: ... Plasmids were isolated from NEB Stable Competent cells using the QIAGEN Plasmid Plus Midi Kit.
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bioRxiv - Biophysics 2024Quote: ... The soluble fraction was bound to amylose resin (NEB, USA) for 1 h and washed with 14 column volumes of wash buffer containing 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... coli BL21(DE3) strain (NEB, Ipswich, MA, USA). The transformants were grown aerobically in LB medium supplemented with kanamycin at 37 C° and 225 rpm overnight (seed culture) ...
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bioRxiv - Cancer Biology 2024Quote: ... and a final concentration determination was performed using NEBNext® Library Quant Kit for Illumina (New England Biolabs Cat. E7630) prior to library sequencing.
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bioRxiv - Biophysics 2024Quote: ... The isolated RNA underwent enzymatic capping (NEB) and polyadenylation (Thermo Fisher) ...
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bioRxiv - Biophysics 2024Quote: ... coli strain (NEB, Ipswich, MA, USA) were used for the gene cloning ...
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bioRxiv - Biophysics 2024Quote: ... Gibson Assembly Cloning Kit (NEB, Ipswich, MA, USA) and 5-alpha E ...
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bioRxiv - Cell Biology 2024Quote: ... RNAseH treatment was performed with 10U of RNAseH (cleaves the RNA when coupled with DNA; NEB) incubated for 2hr at 37°C in a dark humid chamber on one the slides ...
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bioRxiv - Cell Biology 2024Quote: ... the DNA is linearized by KpnI-HF from Biolabs. The linearized DNA was concentrated to 1 μg/μL by ethanol precipitation.
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bioRxiv - Cell Biology 2024Quote: ... PCR reactions were performed with Q5 polymerase (New England Biolabs) in the buffer provided ...
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bioRxiv - Cell Biology 2024Quote: Plasmids were transformed in BL21 (DE3) competent Escherichia coli (New England Biolabs) or Rosetta (DE3 ...
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bioRxiv - Biophysics 2024Quote: ... was obtained from Addgene (#31207) and subsequently inserted into a pGEX-6p-1 vector (Cytiva, 28954648) using Gibson Assembly (New England Biolabs, E2611L). The resulting construct was expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Biophysics 2024Quote: ... coli chemically-competent cells (New England BioLabs). Single colonies of transformed cells from agar plates containing 100 μg/mL ampicillin and grown at 37 °C were used for starter cultures using 50 mL LB media in 250 mL shake flasks containing 100 μg/mL ampicillin ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids were constructed by assembling DNA fragments amplified by PCR into restriction endonuclease-digested vectors using NEB Hifi Builder (NewEngland BioLabs; cat.no: E2621L). The plasmids were confirmed with restriction digestion and sequencing.
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bioRxiv - Cell Biology 2024Quote: ... or (Q71L for Arf1) and dominant negative (T27N for Arf6) or (T31N for Arf1) by using site-directed mutagenesis (SDM kit from NEB) kit ...
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bioRxiv - Cell Biology 2024Quote: ... or N-terminus of PEmax (PEmax-hP53DD-P2A-hMLH1dn) under the constitutive expression from a CMV promoter using Hifi DNA assembly (NEB). To generate the other two all-in-one prime editor with linkages in between the three components ...
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bioRxiv - Cell Biology 2024Quote: ... while lenti-pegRNA-GG-acceptor or lenti-tmpknot-GG-acceptor was digested with BsmBI-v2 (NEB). The pegRNA or epegRNA plasmid was cloned using Golden-Gate assembly ...
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bioRxiv - Cell Biology 2024Quote: ... pU6- or pU6-tmpknot-GG-acceptor was digested with BsaI-HFv2 (NEB), while lenti-pegRNA-GG-acceptor or lenti-tmpknot-GG-acceptor was digested with BsmBI-v2 (NEB) ...