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Citations for New England Biolabs :
3301 - 3350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... DNA fragments encoding CDRs 1 and 2 were digested overnight with BsaI-HFv2 and BbsI-HFv2 respectively (NEB). Reactions were cleaned up with Macherey Nagel Gel cleanup columns ...
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bioRxiv - Biochemistry 2024Quote: ... Digestion was set up to remove the 6X-His-tag by incubating with either homemade or store bought (NEB, Cat. no: P8070L) enterokinase in the presence of 10mM CaCl2 at 22°C for 16h ...
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bioRxiv - Bioengineering 2024Quote: ... The genes were diluted to 125 pM in 30 µL PURExpress cell-free expression mix (NEB) containing 0.06 % pluronic acid (F-127 ...
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bioRxiv - Bioengineering 2024Quote: ... 1 µL 10 mM dNTPs (NEB), 2 µL each 10 µM forward and reverse primers ...
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bioRxiv - Bioengineering 2024Quote: ... containing uracil and overhangs compatible with Thermolabile USER II digestion/ligation (NEB). Following outPCR ...
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bioRxiv - Bioengineering 2024Quote: ... and GamS (NEB), as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... PCR replication of Geneblocks was conducted using Q5® Hot Start High-Fidelity Master Mix (NEB; as per the manufacturer’s instructions ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... Recombinant Muc1 or lubricin mixed with one of the following enzymes: 8 unit/mL of proteinase K (P8107S, New England Biolabs), 500 μg/mL of pronase (10165921001 ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... and treated with 10 mM DTT and Proteinase K (New England Biolabs) at 8 units/mL in digestion buffer (50 mM Tris (pH 8) ...
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FACS-Sortable Picoreactors for Ultra High-throughput Screening of Catalysts in Biphasic EnvironmentsbioRxiv - Bioengineering 2024Quote: ... the two inner solutions were comprised of 1) PURExpress reagents (NEB) or 2 ...
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bioRxiv - Bioengineering 2024Quote: ... coli cells (New England Biolabs), and grown in 5 ml culture overnight at 37C ...
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bioRxiv - Bioengineering 2024Quote: ... Transcription reactions using the Hiscribe IVT kit (New England Biolabs), were assembled to a final volume of 20 μl ...
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bioRxiv - Biochemistry 2024Quote: ... followed by T4 DNA ligase (New England Biolabs) to generate a C-terminal His-tag preceded by a TEV protease cleavage site ...
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bioRxiv - Cancer Biology 2024Quote: ... 2ul mRNA decapping enzyme (New England Biolabs M0608S), and 1ul RNase inhibitor and incubating at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2024Quote: The ATP binding mutants (APLFD11A, APLFR37A, APLFK95A) were generated using Q5® Site- Directed Mutagenesis Kit (NEB #E0554) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and end-repair and ligation was performed using New England Biolabs (NEB) adapters ...
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bioRxiv - Biochemistry 2024Quote: ... Wildtype PLpro and variants were transformed into BL21/DE3 (NEB) and grown in Super Broth (WEHI Bioservices ...
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bioRxiv - Biochemistry 2024Quote: ... Three fragments were cloned in a single HiFi Assembly reaction (NEB). 1 ...
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bioRxiv - Biochemistry 2024Quote: ... The Assembly reactions were transformed into DH-10b electro-competent cell (NEB) and split into 2% and 98% before spreading on Agar plates containing 50ng/ul Kanamycin ...
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bioRxiv - Bioengineering 2024Quote: ... 10 ul rCutSmart Buffer (NEB). The mixes of retrons were organized according to their relative production to retron-Eco1 ...
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bioRxiv - Bioengineering 2024Quote: ... using terminal deoxynucleotidyl transferase (TdT) (NEB). This reaction was carried out in 1× TdT buffer ...
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bioRxiv - Bioengineering 2024Quote: ... 1 ul of RNaseH (NEB), 10 ul rCutSmart Buffer (NEB) ...
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bioRxiv - Bioengineering 2024Quote: ... and 10 units of XbaI (New England Biolabs, Ipswich, MA, USA) in a 25 µL reaction ...
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bioRxiv - Biochemistry 2024Quote: ... by Gibson Assembly (New England Biolabs). The Tspan12 LEL (residues 118-218) ...
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bioRxiv - Plant Biology 2024Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs Cat. No. E7645) and the NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Cat ...
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bioRxiv - Plant Biology 2024Quote: ... in an N-terminal fusion (MIROs are tail anchored proteins) with mCherry that was generated by Gibson assembly (New England Biolabs, Ipswich ...
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bioRxiv - Plant Biology 2024Quote: ... In-frame deletions and substitutions were generated using a Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Hitchen UK) using the primers listed in Table S1 ...
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bioRxiv - Neuroscience 2024Quote: ... 100 ng of total RNA from each sample was used for preparing the libraries with the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs, #E7760), using the rRNA depletion module ...
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bioRxiv - Physiology 2024Quote: ... followed by RNA purification using a Monarch RNA Cleanup Kit following the manufacturer’s protocol (New England Biolabs, Whitby, ON, Canada). One-day old male and female adult mosquitoes were briefly anesthetized using CO2 and injected in the thorax with 1 μg of AedaeItp ...
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bioRxiv - Plant Biology 2024Quote: ... 36 sequencing libraries were obtained using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA), followed by PE 150 sequencing on the Illumina Novaseq 6000 ...
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bioRxiv - Plant Biology 2024Quote: ... RNA sequencing was executed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2024Quote: Fragment corresponding to the coding sequence of full-length Aux/IAAs from Arabidopsis thaliana and the 4C/S-IAA3/SHY2 coding sequence (generated by site directed mutagenesis of AtIAA3/SHY2 coding sequence) was cloned in pMal c5X vector (New England Biolabs) and expressed in BL21 (DE3 ...
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bioRxiv - Plant Biology 2024Quote: ... or amylose resin (negative control; New England Biolabs) at 4°C for 3 h ...
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bioRxiv - Plant Biology 2024Quote: ... While BbsI-HF® (New England BioLabs, USA) was used for Level 0 assembly ...
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bioRxiv - Plant Biology 2024Quote: ... with Luna Universal qPCR Master Mix (New England Biolabs). The cycling parameters were 1 min at 95°C ...
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bioRxiv - Plant Biology 2024Quote: ... BsaI-HF®v2 (New England BioLabs, USA) was used for Level 1 and 3 assembly and SapI (New England BioLabs ...
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bioRxiv - Plant Biology 2024Quote: ... qPCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs, Ipswich MA) and the ThermoFisher StepOnePlus Real-Time PCR system to quantify the expression of GTL1 and the leaf proliferation stage marker ANGUSTIFOLIA 3 (AN3) ...
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bioRxiv - Plant Biology 2024Quote: ... and the supernatant was mixed and incubated with chitin beads (New England Biolabs). The PSM polypeptides were eluted with 50 mM DTT to cleave the intein ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were constructed using Ultra II DNA kit (NEB).
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bioRxiv - Plant Biology 2024Quote: cDNA libraries with different insert sizes were constructed using the NEB Next Ultra TM Directional RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations and were assessed on an Agilent Bioanalyzer 2100 system ...
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bioRxiv - Plant Biology 2024Quote: ... and ligated overnight at 16°C (T4 DNA ligase; NEB). The same approach was used for the second deletion (353 bp ...
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bioRxiv - Plant Biology 2024Quote: ... RNA-Seq libraries were prepared by NebNext Ultra II RNA library preparation kit (New England Biolabs, Massachusetts, USA) and sequenced using Illumina’s state-of-the-art NovaSeq 6000 V1.5 platform (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... was used for Level 1 and 3 assembly and SapI (New England BioLabs, USA) for Level 2 assembly ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were prepared using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Japan, Tokyo, Japan) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... The target loci in ERF74 and GAI genes were PCR amplified using Phusion High-Fidelity DNA polymerase (New England Biolabs) and gene-specific primers (Supplementary Table S1) ...
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bioRxiv - Plant Biology 2024Quote: ... when OD600 reached 0.5 for 4 h at 37 °C and then they were purified using amylose resin column (New England Biolabs, for MBP related proteins purification) or Ni-NTA agarose column (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... polyA-enriched RNASeq libraries were prepared using a PolyA selection NEBNext Ultra II RNA kit (New England BioLabs, Ipswich MA) and sequenced on the Illumina NovaSeq S4 sequencing platform (2×150 bp reads ...
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bioRxiv - Plant Biology 2024Quote: ... the Luna® Universal qPCR Master Mix (New England Biolabs) was used ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized from 1 µg total RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB) with d(T)23VN primer.
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bioRxiv - Plant Biology 2024Quote: ... PCRs were performed with the Q5® High Fidelity DNA Polymerase (NEB) and specific ...