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Citations for New England Biolabs :
3251 - 3300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... digesting with DpnI (NEB R0176L), and assembling by isothermal assembly ...
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bioRxiv - Bioengineering 2024Quote: ... qPCR reactions were set up using the Luna Universal qPCR Master Mix (NEB M3003S). Reactions included 6 µL of Luna Mastermix ...
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bioRxiv - Bioengineering 2024Quote: Q5 DNA Polymerase was used for DNA amplification and colony PCR screening (New England Biolabs, Germany). Gibson Assembly and site-directed mutagenesis was performed using the NEBuilder HiFi DNA Assembly Cloning Kit (E5520S ...
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bioRxiv - Bioengineering 2024Quote: ... This fragment was cloned into a lentiviral expression plasmid we have previously described (31) encoding the Notch1 core linked to the tTA transcription factor using NEBuilder HiFi DNA Assembly (New England Biolabs). The resultant plasmid encoded constitutive expression of CII-synNotch receptor from an EF1α promoter and production of the puromycin N-acetyl-transferase transgene via the constitutive PGK promoter for antibiotic selection ...
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bioRxiv - Bioengineering 2024Quote: cDNA of each library was generated using oAS345 (Supplemtary Note 21) and LunaScript Primer-Free RT Master Mix Kit (NEB E3025S).
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bioRxiv - Bioengineering 2024Quote: ... The bacterial pellet was then resuspended in 1X DNA/RNA protection reagent supplied with the Monarch Total RNA Miniprep Kit (NEB #T2010) (New England BioLabs, USA) and subjected to mechanical lysis using the FastPrep-24™ 5G bead beating grinder system (MP Biomedicals Germany GmbH) ...
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bioRxiv - Bioengineering 2024Quote: ... The LunaScript Primer-Free RT Master Mix Kit (NEB E3025S) was used to generate cDNA per manufacturer protocol ...
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bioRxiv - Bioengineering 2024Quote: ... All A-packaged effector plasmids were generated using an isothermal assembly reaction (NEB M5520AA) (13).
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bioRxiv - Bioengineering 2024Quote: ... Cleaved products were incubated with proteinase K (NEB, Ipswich, MA), A-tailed ...
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bioRxiv - Bioengineering 2024Quote: ... DNA circularization was performed using T4 DNA ligase (NEB, Ipswich, MA), followed by treatment with a mixture of exonucleases to remove residual linear DNA ...
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bioRxiv - Bioengineering 2024Quote: ... USER enzyme (NEB, Ipswich, MA) and T4 polyncucleotide was then used to treat the gap-repaired DNA ...
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bioRxiv - Bioengineering 2024Quote: ... PCRs were carried out using the Q5 Hot Start 2x Master Mix (NEB, Ipswich, MA) according to the manufacturer’s protocol for 30 cycles ...
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bioRxiv - Bioengineering 2024Quote: ... ligated with a hairpin adapter (NEB, Ipswich, MA), and treated with USER enzyme (NEB ...
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bioRxiv - Cell Biology 2024Quote: ChIP-seq libraries were prepared independently from two ChIP biological replicates using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E6177L) according to manufacturer’s instructions with a few modifications ...
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bioRxiv - Cell Biology 2024Quote: ... Library samples were enriched with 11 cycles of PCR amplification in 50uL of total reaction volume (10uL 5x KAPA buffer, 1.5uL 10 mM dNTPs, 0.5uL 10 mM NEB Universal PCR primer ...
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bioRxiv - Cancer Biology 2024Quote: ... All ngRNAs were cloned into pLKO.5 Puro-2A-RFP between BsmBI (New England Biolabs) cut sites ...
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bioRxiv - Cancer Biology 2024Quote: ... This cassette was placed in an anti-sense orientation between two LoxP sequences and a WPRE signal and cloned into the pTwist+lenti+SFFV backbone (Twist Biosciences) between BamHI and XhoI (New England Biolabs) cut sites ...
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bioRxiv - Cancer Biology 2024Quote: ... containing the K700E mutation (AAA>GAA) plus 350bp of upstream and downstream sequences was amplified using Q5 polymerase (New England Biolabs) and cloned into pUC19 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... between HindIII and BamHI (New England Biolabs). The pUC19-SF3B1-K700E plasmid is also used as a double-stranded DNA (dsDNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of RNA were transcribed with reverse-transcription (M-MuLV Reverse Transcriptase, NEB). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... between BbsI (New England Biolabs) cut sites to produce the SF3B1 sgRNA Cas9-GFP construct ...
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bioRxiv - Cancer Biology 2024Quote: ... and then incubated with 2ul of DNase I (New England Biolabs), 2ul RNase A (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... between BsaI (New England Biolabs) cut sites as described26 ...
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bioRxiv - Cancer Biology 2024Quote: ... Base editing sgRNA directed against the SF3B1 K700 locus was designed using BE-Designer28 and cloned into pLKO.5 Puro-2A-GFP between BsmBI (New England Biolabs) cut sites ...
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bioRxiv - Cancer Biology 2024Quote: ... the hU6-pegRNA2-polyT cassette was amplified from the pU6-pegRNA-gg-acceptor backbone and ligated into ngRNA1 pLKO.5 Puro-2A-RFP between EcoRI and XhoI (New England Biolabs) to create pLKO.5-K700E-ng1+pg2-Puro-2A-RFP ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR reaction was mixed by combining 25 μL of Q5 High-Fidelity DNA Polymerase (New England Biolabs #M0494S), 2 μL of the oligo pool solution ...
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bioRxiv - Biochemistry 2024Quote: ... Uracil was removed by uracil DNA glycosylase (0.025 U/μl, New England Biolabs) at 37 °C for 15 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 4 μL of t7 polymerase (New England Biolabs #M0251S), 33 μL RNase-Free water ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/ml recombinant albumin (New England Biolabs), and 0.1 mg/ml RNase A (QIAGEN)] ...
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bioRxiv - Biochemistry 2024Quote: ... Fragments were ligated together with NEBuilder HiFi DNA Assembly Kit (New England Biolabs). Then Escherichia coli DH5α competent cells were transformed with the ligation mixtures and the cells containing assembled plasmid were selected on an agar medium supplemented with 100 mg/l ampicillin ...
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bioRxiv - Biochemistry 2024Quote: ... and ligated together with NEBuilder HiFi DNA Assembly Kit (New England Biolabs). Escherichia coli DH5α competent cells were transformed with the ligation mixtures and the cells containing assembled plasmid were selected on an agar medium supplemented with 100 mg/l ampicillin ...
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bioRxiv - Biochemistry 2024Quote: ... followed by cDNA synthesis with oligoT primers (ProtoScript II, New England Biolabs). The TaPOR2 gene lacking the part coding a transit peptide and pET15b plasmid (Novagen ...
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bioRxiv - Bioengineering 2024Quote: ... – 400 U/mL (NEB); Jack bean hexosaminidase (JBH ...
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bioRxiv - Bioengineering 2024Quote: ... A mastermix containing 2 mmol/L of each dNTP (NEB, Ipswich, MA), 4.5 µg of random hexamers (ThermoFisher ...
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bioRxiv - Bioengineering 2024Quote: ... each sample was also subjected to treatment with DpnI (NEB catalog # R0176) which selectively cleaves methylated GATC sites ...
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bioRxiv - Bioengineering 2024Quote: ... were each treated with a mixture of PNGase F (NEB, Boston, MA, USA) following the manufacturer’s protocol.
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bioRxiv - Bioengineering 2024Quote: ... The resultant PCR product was reassembled into a tdTomato-containing vector via Gibson assembly (NEB) with a tdTomato insert (sequence ordered as a codon-optimized gBlock from IDT ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were amplified using inner primers ACL-P1060 and ACL-P1061 in a 40μl PCR reaction using Q5 Hot Start 2x Master Mix (New England Biolabs) for 20-25 cycles at an annealing temperature of 64°C ...
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bioRxiv - Biochemistry 2024Quote: ... SNAP-Capture magnetic beads (S9145S) were from NEB (Ipswich, MA). Salt Active Nuclease (SAN ...
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bioRxiv - Biochemistry 2024Quote: ... The washed beads were then washed once with LSW once before resuspending in 12 μl LSW with 600 units of micrococcal nuclease (MNase, NEB M0247S) and incubated for 2 min ...
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bioRxiv - Biochemistry 2024Quote: ... coli T7Express cells (New England Biolabs) cells transformed with a pET28a expression vector containing the α ...
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bioRxiv - Biochemistry 2024Quote: ... After 30 mins the samples were placed on ice and 2 μl loading dye (Purple gel loading dye, no SDS, B7025 New England Biolabs) was added prior to loading 12 μl onto a 1.5% 15 x 15 cm 100 ml 0.5X TB agarose gel ...
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bioRxiv - Biochemistry 2024Quote: ... coli T7Express cells (New England Biolabs) as the N-terminal His6-tagged protein ...
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bioRxiv - Biochemistry 2024Quote: ... was 5’ end labelled in a final volume of 10 μl using 0.6 μl T4 PNK (10U/μl, M0201 New England Biolabs) with 2 μl gamma 32P ATP 3000Ci/mmol (SRP-301 Hartmann) ...
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bioRxiv - Biochemistry 2024Quote: ... 13 nt long RNA was radiolabelled at the 5’-end with [γ-32P] ATP and T4 Polynucleotide kinase (New England Biolabs) prior to complexes assembly ...
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bioRxiv - Biochemistry 2024Quote: ... 4SU-labeled RNA (100 µg) was biotinylated with HPDP-biotin (cat. 21341, ThermoScientific) and captured on 150 µL of streptavidin magnetic beads (NEB) per RNA sample ...
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bioRxiv - Biochemistry 2024Quote: ... Isolated MBP-tagged VPS26C was purified using Amylose beads (New England Biolabs) and eluted using 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... the concentrated protein at 3 mg/mL was incubated with c-AMP Protein Kinase A PKA (NEB) (molar ratio of 40:1 ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 mM DNA oligo in T4 polynucleotide kinase buffer (NEB). This mixture was then incubated at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... The ATP and ADP makers were created using T4 polynucleotide kinase (NEB). T4 PNK mixed with 128 µM ATP supplemented with 10 nM [α-32 P]-ATP ...