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Citations for New England Biolabs :
101 - 150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The pLenti vector was digested with BamHI (NEB #R0136) and XbaI (NEB #R0145 ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 mM DTT) with 100 units of MNase (NEB), incubated at 37℃ for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg total RNA was subjected to poly(A) mRNA isolation according to the manufacturer’s protocols (NEB, #E7490). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR reaction was carried out in the volume of 50 µL including 25 µL 2X OneTaq Master Mix (NEB, #M0482), 2 µL of 5 µM i5 and i7 each ...
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Microsatellite break-induced replication generates highly mutagenized extrachromosomal circular DNAsbioRxiv - Molecular Biology 2024Quote: ... Inverse PCR (iPCR) was performed on undigested total genomic DNA using Q5 HotStart polymerase (New England Biolabs, M0494S). The primers used for amplification are shown in Supplementary Table 2 ...
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bioRxiv - Molecular Biology 2024Quote: ... or PmeI (NEB, Cat.R0560L), or BamHI (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... and ∼ 500 ng were digested with DpnI (10 U, NEB, R0176L) in CutSmart Buffer 1X (8h 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... or AsiSI (NEB, Cat.R0630L) or PmeI (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 5.5 mL of Rnase A (NEB, Cat. T3018L) and 5.5 mL of RNase If (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng of a 1kB Plus DNA ladder (NEB, Cat.N3200) was included as a size reference ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 1 μg of genomic DNA was restricted with HinfI (NEB, Cat.R0155M) and RsaI (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... an optional PBS wash followed by Red Blood Cell lysis step was included (10 mins at RT) prior to hypotonic lysis with Nuclei Prep Buffer (NEB, Cat.T3052) and Rnase digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5.5 mL of RNase If (NEB, Cat. M0243L) per million cells for 15 seconds and mixed by flicking ...
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bioRxiv - Molecular Biology 2024Quote: TeloTags were prepared in 100μL reactions with 5mM of each of the 6 permutations of telomere splint Extended Data Fig.1A) and 30 mM of biotinylated adapter in HiFi Taq DNA Ligase Reaction Buffer (NEB, Cat. M0647S). Annealing was done by heating to 99 degrees and slowly decreasing the temperature 1°C /min in a Veriti™ 96-Well Thermal Cycler (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... A total of 40 μg of gDNA was incubated with 3μl of ClaI (NEB, Cat. R0197S) or AsiSI (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... fresh or frozen cell pellets were osmotically lysed in presence of 150mL of Nuclei Prep Buffer (NEB, Cat.T3052) supplemented with 5.5 mL of Rnase A (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... PacI (NEB, Cat. R0547L) or EcoRI (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was isolated using a Monarch Total RNA Miniprep Kit (NEB), RNA quantity and quality were determined with a NanoDrop OneC Spectrophotometer (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... The UbC-Fluc2-SV40 pA cassette was synthesized as DNA gBlocks (Integrated DNA Technologies, IDT) and inserted by Gibson assembly using NEBuilder HiFi assembly mix (New England Biolabs). Unless otherwise noted ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Luna Universal One-Step RT-qPCR Kit (NEB), and then analyzed with QuantStudio Design & Analysis Software v.1.5.1 ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were cloned into the NdeI-BamHI sites of pGBKT7 or pGADT7 vectors using NEBuilder (New England BioLabs, USA).
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bioRxiv - Molecular Biology 2024Quote: ... 3 μl of PvuI (NEB, Cat. R3150S) PacI (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... or EcoRI (NEB, Cat.R0101M), and incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and assembled with HindIII-digested pUC19mPheS plasmid using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs Inc.). The resulting plasmid was then transformed into E.coli DH10b strain for isolation via QIAprep Plasmid Mini-Prep Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... were PCR-amplified and subcloned into the Halo pcDNA5/FRT vector using Gibson Assembly Cloning Kit [New England Biotechnologies (NEB)] ...
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bioRxiv - Molecular Biology 2024Quote: Library preparation for RNA sequencing was performed using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, USA, Catalog #: E7530L), strictly adhering to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared as in [34] with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB).
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Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organismbioRxiv - Molecular Biology 2024Quote: ... 2 mM vanadyl-ribonucleoside complex (New England Biolabs), and 10.6% dextran sulfate (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs). The resulting PCR products were then ligated into the EcoRI/KpnI sites of the pCM29-sgfp vector using the ClonExpress II One-Step Cloning Kit (Vazyme) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA sequencing libraries were created using the NEBNext mRNA stranded protocol (New England Biolabs) and 100 base paired-end sequencing was performed on an Illumina NovaSeq 6000 (McGill University and Genome Quebec Innovation Centre) ...
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Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organismbioRxiv - Molecular Biology 2024Quote: ... coli tRNA (New England Biolabs), 15% formamide (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl of Luna® Universal qPCR Master Mix (Biolabs, Ipswich, MA, USA) and 2 μl from 1:20 diluted cDNA template.
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using primers containing the homology arm flanking the sgRNA region (replacing the BRD4 homology arm) and Gibson cloned (NEBuilder Hifi DNA assembly kit, NEB) into the same vector by digesting with MluI restriction enzyme.
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bioRxiv - Molecular Biology 2024Quote: ... and 0.2 µM of each primer were amplified with 0.5 units of One Taq DNA polymerase (Biolabs, Ipswich, MA, USA). Cycling conditions were established with an initial denaturation step at 95°C/2 min followed by 40 cycles at 95 °C/45 s ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of DNA template in 25 µl of 1x One Taq Standard Buffer (Biolabs, Ipswich, MA, USA), 0.2 mM dNTPs ...
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bioRxiv - Cancer Biology 2024Quote: ... CUT&Tag libraries were prepared with NEBNext HiFi 2x PCR Master Mix (NEB M0541S) and indexed primers (Buenrostro et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... sgRNA was treated with DNase (NEB) and purified using the RNA Clean and Concentrator kit (Zymo Research ...
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bioRxiv - Molecular Biology 2024Quote: RNA was isolated from different tissue (7dpf larvae, adult brain, oocytes and ovary) using Trizol (NEB) and purified using the RNA Clean and Concentrator kit (Zymo Research ...
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bioRxiv - Molecular Biology 2024Quote: ... and used for in-vitro transcription using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). Finally ...
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bioRxiv - Molecular Biology 2024Quote: ... The biotin handle and cosmid-I95 DNA were both digested for 2 h at 37 °C with SpeI-HF (New England Biolabs, R3133L) and subsequently heat-inactivated for 20 min at 80 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... For lambda phosphatase (New England BioLabs, P0753S) treatment ...
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bioRxiv - Microbiology 2024Quote: ... All LAMP reactions were conducted in the WarmStart® Colorimetric LAMP Master Mix (NEB Canada, Whitby, ON). Each 20-µ L reaction contained 2 µ L of template ...
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bioRxiv - Molecular Biology 2024Quote: ... to enrich mRNA and the Ultra II Directional RNA Library Prep Kit (NEB, Cat# E7760) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... rRNA depletion was achieved using the NEBNext rRNA depletion kit v2 (NEB, Cat# E7400L), and subsequent end-repair ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation utilized the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB Cat# E7300/7580) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries for ribosome profiling were prepared following the NEBNext Multiplex Small RNA Library Prep kit for Illumina’s protocol (NEB, Cat# E7300S). Ribo-seq ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by end repair with T4 Polynucleotide Kinase (T4 PNK; NEB, Cat# M0201) at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... the lentiCRISPR-v2 vector was digested by BsmBI-v2 (NEB, Cat# R0739L) and dephosphorylated by Quick CIP (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... ribosome foot-printing involved the addition of 1.25 U/μL RNase I (NEB Cat# M0243L) to 500 μL clarified lysate ...
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bioRxiv - Molecular Biology 2024Quote: ... with NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520S). The details of plasmids source and usage are listed in Supplementary Table 4 ...