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Citations for New England Biolabs :
3201 - 3250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The mRNA was purified using the Monarch RNA Cleanup Kit (NEB, MA, UK). Followed reagents were used for IL-15 superagonist characterization ...
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bioRxiv - Cancer Biology 2024Quote: ... that has been previously digested with XbaI (New England Biolabs, Bioconcept) and XmaI ...
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bioRxiv - Bioengineering 2024Quote: ... Synthesized mRNA was purified using the Monarch RNA cleanup kit (New England Biolabs) and stored at −80°C ...
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bioRxiv - Bioengineering 2024Quote: ... single indices for demultiplexing were added using the NEBNext Multiplex Oligos for Illumina (NEB, E7335S, E7500S, or E7710S) in 6-cycle PCR ...
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bioRxiv - Bioengineering 2024Quote: High Molecular Weight (HMW) DNA was obtained using the Monarch® HMW DNA extraction kit for Tissue (T3060L, New England Biolabs), as previously described (Low et al ...
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bioRxiv - Bioengineering 2024Quote: ... The RNA template of the SARS-CoV-2 N gene was synthesised using in-vitro transcription (Hiscribe, NEB, US) and a template plasmid (Molecular Diagnostics Collection ...
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bioRxiv - Bioengineering 2024Quote: ... Typhi (the bacterial pathogen that causes typhoid fever) using 2X Warmstart colorimetric LAMP (M1800, NEB, US). The RNA template of the SARS-CoV-2 N gene was synthesised using in-vitro transcription (Hiscribe ...
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bioRxiv - Bioengineering 2024Quote: ... Target cDNA were then amplified using one primer that annealed specifically to the target RNA and a second primer that annealed specifically to the rcRNA (primers 45/46 for dmd and primers 47/48 for lmna) with Q5® High-Fidelity DNA Polymerase (New England Biolabs) for 35-cycles.
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bioRxiv - Bioengineering 2024Quote: ... The splitGFP plasmid was amplified using primers 53 and 54 to install 3’ stop codon and primers 55 and 56 to install the 5’ stop codon using Q5® High-Fidelity DNA Polymerase (New England Biolabs) for 35-cycles ...
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bioRxiv - Bioengineering 2024Quote: ... Guide RNA spacers were cloned into rcRNA vectors by digesting plasmids with Esp3i restriction enzyme (New England Biolabs) for 1 hour at 37°C according to manufacturer’s directions ...
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bioRxiv - Bioengineering 2024Quote: ... Target cDNA were then amplified using primers that span the trans-splice junction (primers 41/42 for dmd and primers 43/44 for lmna) and predicted mutation with Q5® High-Fidelity DNA Polymerase (New England Biolabs) for 25-cycles ...
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bioRxiv - Bioengineering 2024Quote: ... Digested backbone plasmid and annealed spacer sequences were assembled using NEB High-Fidelity DNA Assembly 2x Master Mix (New England Biolabs) in a reaction containing 5 µl of the master mix 2 µl of the digested backbone and 3 µl of the PCR annealed spacer ...
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bioRxiv - Bioengineering 2024Quote: ... Primers containing the spacer sequence and homology to the rcRNA plasmid backbone were annealed using Q5® High-Fidelity DNA Polymerase (New England Biolabs) for 35-cycles ...
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bioRxiv - Bioengineering 2024Quote: ... The bacterial pellet was then resuspended in 1X DNA/RNA protection reagent supplied with the Monarch Total RNA Miniprep Kit (NEB #T2010) (New England BioLabs ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA extraction using Monarch® Genomic DNA Purification Kit (New England Biolabs), and histology sectioning.
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bioRxiv - Bioengineering 2024Quote: ... 0.32 U/µL glycerol-free WarmStart Bst 2.0 (NEB #M0402B-BM), 0.3 U/µL glycerol-free WarmStart RTx (NEB #M0439B-HC1) ...
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bioRxiv - Bioengineering 2024Quote: ... The assays are composed of 1X Isothermal Buffer (NEB #B0537), 6 mM Magnesium Sulfate (MgSO4 – NEB #B1003) ...
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bioRxiv - Bioengineering 2024Quote: ... 6 mM Magnesium Sulfate (MgSO4 – NEB #B1003), 1.4 mM deoxynucleotide (dNTP ...
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bioRxiv - Bioengineering 2024Quote: ... 1.4 mM deoxynucleotide (dNTP) mix (NEB #N0447), 0.32 U/µL glycerol-free WarmStart Bst 2.0 (NEB #M0402B-BM) ...
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bioRxiv - Bioengineering 2024Quote: ... 0.3 U/µL glycerol-free WarmStart RTx (NEB #M0439B-HC1), 1X Chai Green (Chai Biosciences #R01200) ...
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bioRxiv - Bioengineering 2024Quote: ... and treated with USER enzyme (NEB, Ipswich, MA). Products were amplified by PCR with the Kapa HiFi polymerase (Kapa Biosystems ...
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bioRxiv - Bioengineering 2024Quote: ... pH = 8 for 16 hours at 56°C followed by 30 minutes at 90°C and an additional digest of 4 units of beta-agarase (M0392S, New England BioLabs) for 1 hour at 65°C to ensure full agarose breakdown ...
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bioRxiv - Biophysics 2024Quote: M2R constructs were dephosphorylated through a 30-minute room temperature treatment with 200 units of lambda protein phosphatase (NEB), 10 units of calf intestinal phosphatase (NEB) ...
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bioRxiv - Biophysics 2024Quote: ... 10 units of calf intestinal phosphatase (NEB), 5 units of antarctic phosphatase (NEB) ...
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bioRxiv - Bioengineering 2024Quote: ... Illumina adapters were ligated on at the 3′ end of the complementary strand using 1× TA Ligase Master Mix (NEB). All products were indexed and sequenced on an Illumina MiSeq instrument ...
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bioRxiv - Bioengineering 2024Quote: ... and Aqp1 were amplified using Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA) and cloned by Gibson assembly in a lentiviral transfer vector under the control of a constitutive promoter ...
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bioRxiv - Bioengineering 2024Quote: ... All PCR amplifications were performed using the Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA).
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bioRxiv - Bioengineering 2024Quote: ... pH 8.0) containing 8 units mL−1 proteinase K (NEB, P8107S) at 37 °C for 4 h ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA extraction using Monarch® Genomic DNA Purification Kit (New England Biolabs), and histology sectioning.
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bioRxiv - Bioengineering 2024Quote: ... Then it was incubated with 20ME (Molar equivalent) of BG-GLA-NHS (NEB) for 30 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... The TSA1 promoter was amplified by PCR from genomic DNA obtained from the X-33 strain using Q5 polymerase (New England Biolabs) and primers TSA1_BstBI_Fw and TSA1-GFP_Rv_KpnI ...
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bioRxiv - Bioengineering 2024Quote: All polymerase chain reaction (PCR) amplification steps were performed using Phusion DNA polymerase (NEB, Whitby, Ontario, Canada). Primers used for PCR amplification were ordered from Integrated DNA Technologies (IDT ...
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bioRxiv - Bioengineering 2024Quote: ... coli DH5α (Matthew Scott Lab, University of Waterloo) and NEB10-β (New England Biolabs (NEB), Whitby ...
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bioRxiv - Bioengineering 2024Quote: ... the DNA templates were linearized by digestion with HindIII and ScaI (NEB) and purified by phenol-chloroform extraction ...
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bioRxiv - Bioengineering 2024Quote: ... coli strains used in this study were DH5α (New England Biolabs) for cloning ...
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bioRxiv - Cancer Biology 2024Quote: ... and treated with 800 U of Lambda Phosphates (New England Biolabs) for 30 min at 30°C ...
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bioRxiv - Bioengineering 2024Quote: ... Transcription reactions were purified using the Monarch RNA cleanup kit (New England Biolabs). mRNA concentration was quantified by nanodrop spectrophotometer ...
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bioRxiv - Bioengineering 2024Quote: ... Transcription templates were made by site directed mutagenesis to substitute the T7 promoter with the promoter of interest on pCMV-Cluc2 (New England Biolabs).
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bioRxiv - Bioengineering 2024Quote: ... anchored primer was used to create a complementary strand to the TdT extended products using 15 units of Klenow Fragment (3′→5′ exo-) (NEB) in 1× NEB2 ...
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bioRxiv - Bioengineering 2024Quote: ... coli DH5α (Matthew Scott Lab, University of Waterloo) and NEB10-β (New England Biolabs (NEB), Whitby ...
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bioRxiv - Bioengineering 2024Quote: ... comprising 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA), 0.2 μM of forward and reverse primers ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products were assembled using NEB Hifi DNA Assembly Master Mix (NEB) according to the manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The pTA-Mob plasmid (gift from Thomas Hamilton and David Edgell, Western University) was linearized using AvrII (NEB) and combined with the amplified fragment from pFAST-PETase-cis using a modified NEB Hifi Assembly protocol with a two-hour assembly step at 50 °C ...
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bioRxiv - Bioengineering 2024Quote: ... or Q5 Hot Start High-Fidelity 2x Mastermix (M0492, NEB) in a 15 μL reaction ...
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bioRxiv - Bioengineering 2024Quote: ... Amplicons from mouse embryo lysates were generated using Q5 Hot Start High-Fidelity 2x Mastermix (M0492, NEB) in a 25 μL reaction ...
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bioRxiv - Bioengineering 2024Quote: ... And cloned into a expression plasmid under the control of the EF1α using NEB High-Fidelity DNA Assembly 2x Master Mix (New England Biolabs). To generate 3’ and 5’ stop codon reporters ...
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bioRxiv - Bioengineering 2024Quote: ... either half of the EGFP open reading frame was amplified from pLHA-TR-EF1α-Hipk3-Polio-SplitGFP45 with primers 49 and 50 (first half of EGFP ORF) and primers 51 and 52 (second half of EGFP ORF) using Q5® High-Fidelity DNA Polymerase (New England Biolabs) for 35-cycles ...
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bioRxiv - Bioengineering 2024Quote: All polymerase chain reactions were performed using Q5 High-Fidelity 2X Master Mix (New England Biolabs) and all primers were synthesized by IDT (Integrated DNA Technologies) ...
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bioRxiv - Bioengineering 2024Quote: LunaScript Primer-Free RT Master Mix Kit (NEB E3025S) was used to generate cDNA using primers oAS344 for cRNA and oAS345 for vRNA (Supplementary Note 21).
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bioRxiv - Bioengineering 2024Quote: ... The mara1 fragment was cloned into pRM4e vector using NEBuilder HiFi DNA Assembly (New England Biolabs) master mix and introduced into E ...