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Citations for New England Biolabs :
3151 - 3200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 2 μL of T4 DNA ligase (NEB M0202S) was added to the mixture and incubated overnight at room temperature to seal nicks on DNA ...
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bioRxiv - Biochemistry 2024Quote: ... 100 μL of 1 unit mL-1 SfoI restriction enzyme (NEB) was injected to generate blunt-end DNA molecules.
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bioRxiv - Biochemistry 2024Quote: ... 200 U of T4 ligase in T4 ligase buffer (NEB), 0.1 mg/mL BSA (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 µL T4 DNA Ligase (NEB #M0202L), 0.5 µL T4 Polynucleotide Kinase (NEB #M0201L) ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/mL BSA (NEB) and 40 GoldenGate digestion ligation cycles (80) ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 µL T4 Polynucleotide Kinase (NEB #M0201L), and 0.5 µL DpnI (NEB #R0176L ...
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bioRxiv - Biochemistry 2024Quote: ... coli (NEB #C2984H ...
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bioRxiv - Biochemistry 2024Quote: ... 200 U of T4 ligase in T4 ligase buffer (NEB), 0.1 mg/mL BSA (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.5 µL DpnI (NEB #R0176L) for 2 hours at 25 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR reaction was DpnI (New England Biolabs) digested at 37 °C for 2 h without prior clean-ups ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... SIS1VR 5’- CCAATCTGTTCGCGGTGAGCCTCA-3’) by Gibson Assembly (NEB). All constructs were confirmed by sequencing.
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bioRxiv - Cancer Biology 2024Quote: ... The transcription products were purified with Monarch RNA Cleanup Kit (NEB) followed by polyadenylation using Poly(U ...
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bioRxiv - Cancer Biology 2024Quote: ... and purification with Monarch RNA Cleanup Kit (NEB). RNAs were then used for MeRIP assays.
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bioRxiv - Cancer Biology 2024Quote: ... PCR-amplified DNA templates encoding 5’UTR of ATF4 mRNA were used for in vitro transcription of ATF4 reporter mRNA using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by polyadenylation using Poly(U) Polymerase (NEB) with Bio-16-UTP (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... and the chromatin was digested using 200 units of HindIII (NEB) at 37 °C overnight and then the digestion was stopped with 1.6% SDS at 65 °C for 20 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Digested DNA fragments were ligated using T4 DNA ligase (NEB) for 4 h at 16 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by PCR amplification of the BC-sgRNA1-sgRNA2 region from 32 μg of bulk lung genomic DNA using Q5 Ultra II High-Fidelity 2× Master Mix (New England Biolabs, M0494X). Unique dual-indexed primers were used to amplify each sample followed by purification using Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Biophysics 2024Quote: ... Plasmids created in this study were assembled using the Gibson Assembly Cloning Kit (New England Biolabs). All plasmid sequences were confirmed by whole plasmid sequences services provided my Plasmidsaurus SNPsaurus LLC.
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bioRxiv - Cancer Biology 2024Quote: cDNA was generated from RNA using components from a NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, cat. no. E6421S), MAS-Seq for 10x Single Cell 3’ kit (PacBio ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was generated from RNA using components from a NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, cat. no. E6421S). The RNA Samples were diluted ...
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bioRxiv - Cancer Biology 2024Quote: ... was digested using the NheI_HF and XmaI (New England Biolabs, Bioconcept) and cloned into the pLenti_CMV_GFP_Blast (Addgene #17445 ...
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bioRxiv - Bioengineering 2024Quote: ... Nluc mRNA was synthesized using the HiScribe T7 high yield RNA synthesis kit (New England Biolabs Inc., MA, USA) with CleanCap Reagent AG (TriLink Biotechnologies ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were inserted into the respective plasmids using the NEBuilder HiFi DNA Assembly system (NEB). All constructs used in this study were confirmed by sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... Before transfection pKD plasmids were digested overnight with EcoRV (NEB). The enzyme was then subjected to heat inactivation for 20 min at 65 °C and then mixed with the pUF1-Cas9 plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... were digested using NheI-HF (R3131S - NEB) and AgeI-HF (R3552S-NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... Ligation of the PCR product with the purified destination vector was performed using T4 DNA Ligase (M0202S - NEB) and transformed in Stbl3 bacteria ...
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bioRxiv - Cell Biology 2024Quote: ... and cloned into pMAL vector (NEB) in frame with an N-terminal maltose binding protein (MBP ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were clarified by centrifugation at 16,000 × g for 45 min and the supernatant was loaded onto amylose affinity resin (New England BioLabs, Ipswich, MA). The column was washed with 10 CVs of lysis buffer and the protein was eluted with sequentially added 1 CV aliquots of lysis buffer Supplemented with 20 mM maltose ...
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bioRxiv - Cell Biology 2024Quote: ... using the Gibson Assembly Master Mix (New England Biolabs). pPM28 was similarly generated by Gibson Assembly of the pHlourin amplicon into pPM32 ...
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bioRxiv - Cell Biology 2024Quote: ... The amplicons were then assembled into pK3F (linearized with SpeI (New England Biolabs)) using the Gibson Assembly Master Mix (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... and areas corresponding to the expected size were excised and purified from the gel using a gel extraction kit (Monarch DNA Gel Extraction Kit, NEB). Following purification ...
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bioRxiv - Cell Biology 2024Quote: ... samples were run on a 1% agarose gel and areas corresponding to the expected size were excised and purified from the gel using a gel extraction kit (Monarch DNA Gel Extraction Kit, NEB, USA). This PCR product was used for a second round of amplification followed by gel purification ...
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bioRxiv - Cell Biology 2024Quote: ... and was reverse transcribed using ProtoScript II reverse transcriptase (New England Biolabs, USA) with primers specific to RPS27A and RPS27AP5 common regions previously used for gDNA amplification ...
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bioRxiv - Cell Biology 2024Quote: ... and PacI (New England Biolabs) and ligated into gel purified pPM29 digested with BamHI and PacI.
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bioRxiv - Cell Biology 2024Quote: ... and XhoI (New England Biolabs) and ligated (T4 DNA ligase ...
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bioRxiv - Cell Biology 2024Quote: ... the pellet was air dried and then resuspended in DNA Elution Buffer (Monarch DNA Gel Extraction Kit, NEB, USA). Since the RPS27AP5 sequence ...
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bioRxiv - Cell Biology 2024Quote: ... The amplicon was restriction digested with BamHI (New England Biolabs) and PacI (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... coli cells (NEB) and identified by colony PCR followed by Sanger sequencing.
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bioRxiv - Cell Biology 2024Quote: ... using NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621). Proteins were transfected into Rosetta2 (DE3 ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was produced from ∼1 µg of RNA using M-MuLV Reverse Transcriptase (New England Biolabs). RTqPCR was performed on 1:10 dilutions of the cDNA using iTaq Universal SYBR Green Supermix (Bio Rad ...
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bioRxiv - Cell Biology 2024Quote: ... and SalI (New England Biolabs). To generate pPM11 ...
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bioRxiv - Cell Biology 2024Quote: ... was fused to the cDNA sequence of mScarlet-I by PCR and subcloned into pMNATZA1 using Gibson assembly with NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA). The spPKA-KTR1.0 is stably expressed under the Padh1 promoter from the Z locus ...
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bioRxiv - Cell Biology 2024Quote: ... The amplicons were digested with SacI and EagI ligated into the plasmid containing the ATG8 promoter linearized with HindIII (New England Biolabs) and SalI (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... site-directed mutagenesis of the codon encoding lysine at amino acid position 41 was altered to alanine using the Q5 site-directed mutagenesis kit (New England Biolabs) using pPM11 as a template ...
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bioRxiv - Cell Biology 2024Quote: ... Monarch® DNA Gel Extraction Kits (NEB) were used per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was amplified using the Luna Universal qPCR Master Mix (New England Biolabs M3003E) as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... and then treated with Exonuclease III (New England Biolabs, #M0206L) at 37°C for 15 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were purified and cloned using Gibson assembly master mix (New England BioLabs) into LRG3.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were constructed with the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs) following the manufacturer’s low DNA protocol ...