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Citations for New England Biolabs :
3301 - 3350 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The PCRs were conducted in volumes of 30 μL comprising a Phusion® High-Fidelity PCR Master Mix (New England Biolabs) (15 μL) ...
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bioRxiv - Microbiology 2023Quote: ... the native barcodes were ligated with the Native Barcoding Expansion 96 (EXP-NBD196) and NEB Blunt/TA Ligase Master Mix (NEB, M0367) selecting a unique barcode per sample ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The resulting three fragments and the vector were fused using the Hifi DNA Assembly Master Mix (New England Biolabs, Ipswich, USA) at 50 °C for 1 h ...
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bioRxiv - Genetics 2022Quote: ... Two independent lines were successfully established and PCR validated through single fly PCR (Gloor et al., 1993) using OneTaq PCR master mix (NEB #M0271L). Primers for PCR were designed to flank the integration site (primer sequences ...
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bioRxiv - Bioengineering 2022Quote: ... PCR was performed in a total volume of 50 μL with 4.0 μg genomic DNA per reaction using NEBNext Ultra II Q5 Master Mix (New England Biolabs, Ipswich, MA) (98 °C for 3 min ...
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bioRxiv - Cell Biology 2022Quote: ... The mutant fragments were amplified by high-fidelity DNA polymerase 2 × Phanta Max Master Mix followed by DpnI (New England BioLabs; R0176S) digestion in 37°C for 1 hour to eliminate the templates ...
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bioRxiv - Cancer Biology 2023Quote: ... CRISPR sgRNA resistant synonymous or functional domain point mutations were introduced by PCR mutagenesis using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2631). Stable cell lines were generated using the pLU vectors and selected with puromycin or blasticidin ...
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bioRxiv - Genomics 2022Quote: ... signal constructs were amplified by PCR from different plasmids and assembled using the NEBuilder HiFi DNA Assembly Master Mix (HiFi Assembly, NEB #E2621). The BxbI attP site was then added using the Q5 Site-Directed Mutagenesis Kit (Q5 SDM ...
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bioRxiv - Physiology 2023Quote: ... The gel-purified PCR products were cloned into the EcoRI- digested pBluescript II SK(+) vector using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs, E2621X). The mixture of pU6b-sgRNA and targeting vector was microinjected into w1118 ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmid assembly was performed by in vitro Gibson assembly using a HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich MA), downscaled to 5 µL reaction volume ...
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bioRxiv - Cell Biology 2023Quote: ... The APOE3 ORF was amplified from APOE3-TurboGFP and subcloned into an mEmerald-N1 backbone via Gibson assembly using HiFi DNA Assembly Master mix (New England Biolabs, E2621) to make APOE3-mEm ...
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bioRxiv - Cell Biology 2023Quote: ... The 1.3 Kb OCT4 insert was ligated into the digested pcDNA3-EGFP vector using the NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The amplified fragments containing overlapping sequences of vector pKG116 were ligated into the digested vector pKG116 using Gibson assembly reaction in NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs). After cloning ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by PCR amplification of the gRNA cassette using primers compatible with Illumina sequencing and NEB Q5 Hot Start High-Fidelity master mix (NEB M0494) (see primers listed in Supplemental Table 1) ...
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bioRxiv - Pathology 2023Quote: ... a synthetic gene fragment containing the Spleen-forming focus virus promoter sequence followed by the iRFP670 protein-coding sequence (gBlock; Integrated DNA Technologies, IDT) was assembled with the gel-purified plasmid using the HiFi Assembly Master Mix (NEB, E5520) disrupting nef sequence (Δnef) ...
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bioRxiv - Microbiology 2023Quote: ... these fragments were assembled along with the previously PCR-amplified vector pR6KTet-SacB using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). The assembled constructs were then transformed by electroporation into E ...
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bioRxiv - Synthetic Biology 2024Quote: ... and both homology arms were ordered as gBlocks from Integrated DNA Technologies (IDT) and assembled (NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs (NEB)) ...
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bioRxiv - Microbiology 2023Quote: ... A 3x HA-tag was fused to the C-terminal region of the amplified product along with the 3’UTR formed through Gibson assembly master mix (NEB, E2611S). To generate the PfMORC-HA knockdown constructs ...
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bioRxiv - Molecular Biology 2023Quote: ... and inserted using the NEBuilder HiFi DNA Assembly Master Mix into the pKH011 plasmid digested with NcoI-HF (NEB, Cat# R3193S) and XbaI (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... as wildtype or T205A variant were amplified by polymerase chain reaction (PCR) using Q5 High-Fidelity master mix (M0492, New England Biolabs (NEB)) from previous plasmid constructs 16 ...
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bioRxiv - Bioengineering 2023Quote: ... This fragment was inserted into the pAAV plasmid above using the Gibson Assembly® Master Mix (New England Biolabs, Ipswich, MA). This “base” plasmid ...
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bioRxiv - Cell Biology 2023Quote: ... We assembled the pLenti-3HA-Clic4-T2A-GFP vector with pLenti backbone and fragments using NEBuilder HiFi DNA Assembly Master Mix (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Double-stranded blunt-end libraries were constructed from the extracted DNA using NEBNext DNA Prep Master Mix Set E6070 (New England Biolabs Inc.) and sequenced (80bp and 100bp single read ...
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bioRxiv - Synthetic Biology 2023Quote: ... in case of multiplexing the EXP-NBD104 extension was combined with NEB Blunt/TA Ligase Master Mix (M0367, New England BioLabs (NEB)) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the targeted DNA region was amplified by PCR using the biomass as the template with the Q5™ High-Fidelity 2× master mix (New England Biolabs). Base-editing efficiency when targeting a plasmid-borne gene was estimated by isolating plasmid DNA upon 24-h editing treatment by using the NucleoSpin™ plasmid EasyPure kit (Macherey-Nagel ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA fragments to be assembled were amplified by PCR using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532S). The PCR mix underwent gel electrophoresis for purification ...
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bioRxiv - Genomics 2024Quote: ... The reaction was purified using the Zymo DNA Clean & Concentrator kit and PCR-amplified with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541L) and primers as defined in Corces et al ...
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bioRxiv - Genomics 2024Quote: ... The library PCR to add the Illumina small RNA barcodes and handles was performed by adding 25 μL NEBNext Ultra II Q5 Master Mix (NEB, M0492L), 11 μL Nuclease-free water and 2 μL RP1 and RPIx primers each (10 mM ...
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bioRxiv - Genomics 2024Quote: ... DNA and cDNA from the three replicates were kept separate and underwent a 3-cycle PCR using NEBNext Ultra II Q5 Master Mix (New England Biolabs, M0544L), forward primer P7-pLSmp-ass16UMI-gfp and reverse primer P5-pLSmP-5bc-i# ...
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bioRxiv - Cell Biology 2024Quote: We fused SNAP-3xHA coding sequences downstream H3.1 and H3.3 CDS 46 and inserted this fusion protein into the pBS31 vector 70 using NEBuilder® HiFi DNA Assembly Master Mix (NEB). These expression vectors (pB31-H3.1- and pB31-H3.3-SNAP-3xHA ...
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bioRxiv - Cancer Biology 2024Quote: All the newly generated plasmids reported in this study were created by Gibson Assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, E2621L) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Another round of PCR was conducted to prepare the library for sequencing using NEBNext Ultra II Q5 Master Mix (New England Biolabs, M0544L), forward primer P5 and reverse primer P7 ...
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bioRxiv - Developmental Biology 2024Quote: ... All amplicons were subcloned into pCS2+ vector via FseI/AscI restriction sites or using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621S). The plasmids containing tp63.S and hoxd1.S were linearized using NotI ...
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bioRxiv - Molecular Biology 2024Quote: ... and the luciferase gene was replaced with the cGAS or EGFP sequences using NEBuilder HiFi DNA Assembly Master Mix (NEB E2621). The pAAV:cTnT::GFP-icGAS vector was generated by inserting the catalytically-inactive cGAS (icGAS ...
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bioRxiv - Cancer Biology 2024Quote: ... Ligation of sequences was performed by PCR-based cloning via addition of specific restriction sites using the Phusion High-Fidelity PCR Master Mix (New England Biolabs, #F531L), restriction digest and subsequent ligation using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... Adaptor-ligated DNA fragments of proper size were enriched with PCR reaction using Phusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The adaptor-ligated DNA on the magnetic beads was amplified by 5-8 cycles of PCR using NEBNext Ultra II Q5 Master Mix (New England Biolabs, M0544) and NEBNext Multiplex Oligos for Illumina (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR adapters from Oxford Nanopore Technologies (ONT #SQK-PSK004) were ligated to end repaired DNA using NEB Blunt/TA Ligase Master Mix (NEB #M0367). 1x MeDIP master mix (10 mM Sodium phosphate buffer ...
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bioRxiv - Biochemistry 2024Quote: ... FOX templates were obtained from Addgene 45 and FOX-DBD DNA sequences were ligated and cloned into the pGEX-6P2 using Gibson Assembly Master Mix (NEB: E2611L). Point mutants were generated with the QuikChange Lightning site-directed mutagenesis kit (Agilent ...
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bioRxiv - Plant Biology 2024Quote: ... The PCR products of the ORF of CrHCF244 and linear vectors were assembled by Gibson assembly (Gibson et al., 2009) using Gibson Assembly® Master Mix (New England Biolabs). For the vector construct to transform the Arabidopsis HCF244 gene into Chlamydomonas ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The vector and the PCR products were then integrated using Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix, New England Biolabs, #E2621S). Clones were sequenced to verify accuracy ...
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bioRxiv - Biochemistry 2024Quote: ... The three amplicons were purified and combined using the NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs, Ipswich, MA, USA) according to the instructions provided by the manufacturer ...
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bioRxiv - Genomics 2024Quote: ... custom iTru adapters were ligated to amplicons with NEBNext® Ultra II Ligation Master Mix (Cat No. E7648, NEB, Ipswich, MA) with a 15 min incubation at 20°C (Supplemental Table 8) ...
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bioRxiv - Synthetic Biology 2024Quote: ... All PCR reactions were carried out in 30 μL reactions with 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers ...
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bioRxiv - Immunology 2024Quote: ... The gel-purified PCR products were cloned into the EcoRI-digested pBluescript II SK (+) vector using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, E2621X). The mixture of pU6b-sgRNA and targeting vector was microinjected into w1118 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and pYFAC-ODC-pyrG were built by isothermal assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, MA, USA). First ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic regions flanking the expected cut sites for the indicated sgRNAs were PCR amplified using Q5 PCR Master Mix (NEB, #M0541L). PCR products were purified using the Monarch PCR and DNA Cleanup Kit (NEB ...
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bioRxiv - Microbiology 2024Quote: ... DNA sequences were amplified and amended with ≥18 bp homology to their destination vectors using Q5 Hot Start High Fidelity Master Mix (NEB, M0494L). Vectors were constructed using Gibson Assembly with HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The mCherry gene was excised from pL1694 by BamHI and NotI and replaced by a human codon-optimised spCas9 from pUF1-Cas912 by Gibson cloning (NEBuilder HiFi DNA Assembly Master Mix, New England Biolabs, NEB) to generate pL1694-GIMO-Cas9.
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bioRxiv - Molecular Biology 2024Quote: ... pFastBac-NSUN2 and pFastBac-NSUN2-K190M vectors has been obtained by Gibson assembly methodology and Gibson Assembly Master Mix (NEB # E2611S). DNA sequences of recombinant plasmids have been determined by Sanger sequencing.