Labshake search
Citations for New England Biolabs :
3451 - 3500 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and cloned into overexpression vectors under act5 promoter with either N-terminal or C-terminal FLAG tags using Gibson Assembly® Master Mix (NEB, E2611L) at 50 °C for 1 hr ...
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bioRxiv - Molecular Biology 2024Quote: The pAAV plasmids generated in this study were produced by Gibson assembly using NEBuilder® HiFi DNA Assembly Master Mix (NEB, E2621S). The AAV-HR for in vitro studies in HEPA1-6 cells was obtained by replacing the hFIX gene5 with the firefly luciferase (Fluc ...
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bioRxiv - Genomics 2024Quote: ... Up to 2.5 µg of genomic DNA was added to each 50 µL PCR reaction with 25 µL of NEBNext Ultra II Q5 master mix (NEB, Cat #M0544L), 1.25 µL of the 10 µM forward primer and 1.25 µL of the 10 µM reverse primer ...
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bioRxiv - Genomics 2024Quote: ... and amplified using ISOSDB412 IS-Seq Step1 and p7 primers for 13 cycles using Q5 Master Mix (New England Biolabs, Ipswich, MA). The products of this reaction were amplified with IS-Seq Step2 and p7 primers for 9 cycles using Q5 Master Mix and sequenced on a Novaseq 6000 at Novogene (Sacramento ...
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bioRxiv - Genetics 2024Quote: ... Cloning was performed by Gibson Assembly of PCR amplified or commercially synthesized gene fragments (from Integrated DNA Technologies or Twist Bioscience) using NEBuilder Hifi Master Mix (NEB Cat# E262), and final plasmids sequence-verified by Sanger sequencing of the open reading frame and/or commercial whole-plasmid sequencing service provided by Primordium.
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bioRxiv - Molecular Biology 2021Quote: ... and resuspended in 2x RNA Loading Dye (NEB). Resuspended RNAs were incubated at 72°C for 5 min and loaded onto a 15% polyacrylamide 7 M urea gel ...
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bioRxiv - Genomics 2021Quote: ... and NEBNext 2x Mastermix (New England BioLabs M0541L) in order to construct separate HTO and ADT libraries ...
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bioRxiv - Molecular Biology 2022Quote: ... and 10 μL 2X RNA Loading Dye (NEB), denatured at 95° for 5 min ...
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bioRxiv - Bioengineering 2024Quote: ... NEBuilder HiFi 2X Mastermix (New England Biolabs; E2621L) was utilized according to the manufacturer’s protocol88 ...
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bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix with GC Buffer (New England Biolabs, Ipswich, MA, USA) and high-fidelity polymerase (New England Biolabs).
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bioRxiv - Microbiology 2019Quote: ... the first PCR was performed in triplicate with tailed forward and reverse primers using NEBNext Q5® Hot Start HiFi PCR Master Mix (New England BioLabs, Hitchin, UK) and 17 and 25 cycles for the bacterial and functional marker ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplified Mmp13 promoter sequences and digested pGL3 were purified using GeneJET PCR Purification Kit and cloned using NEBuilder HiFi DNA Assembly Master Mix (E2621L, NEB, Ipswich, MA, USA). Mutations in Mmp13 promoter SNPs were generated through site-specific mutagenesis PCR with the mutations in the primers (Ho et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... The amplified Mmp13 promoter sequences and digested pGL3 were purified using GeneJET PCR Purification Kit and cloned using NEBuilder HiFi DNA Assembly Master Mix (E2621L, NEB, Ipswich, MA, USA). Mutations in Mmp13 promoter SNPs were generated through site-specific mutagenesis PCR with the mutations in the primers (Ho et al. ...
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bioRxiv - Systems Biology 2022Quote: ... 100ng of vector backbone and 20ng of trcrRNA-CaptureSeq-5’LTR(truncated) cassette were combined with 10 μl of NEBuilder HiFi DNA assembly master mix (NEB cat. no. E2621L) and water to 20 μl ...
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bioRxiv - Molecular Biology 2021Quote: Pooled sgRNA oligonucleotides were synthesized as 76-mers by Custom Array (Bothell, WA, USA) and were amplified by PCR with NEBNext High Fidelity PCR Master Mix (New England BioLabs, NEB, Ipswich, MA, USA) using customized primers (Additional file 2 ...
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bioRxiv - Genomics 2020Quote: ... Samples were then end-repaired (NEBNext End repair/dA-tailing Module) and ligated to ONT 1D adapters (NEB Blunt/TA Ligase Master Mix).
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bioRxiv - Molecular Biology 2021Quote: ... These two DNA fragments were combined with BamHI digested pFA6a-natNT2 plasmid using NEBuilder HiFi DNA Assembly Master Mix (E2621, New England Biolabs, Ipswich, MA, USA). The resulting plasmid (pHM1119 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cloning was carried out using the Gibson Assembly® Master Mix kit and NEBuilder® HiFi DNA Assembly kit (New England Biolabs, US). Genomic DNA was prepared by the Blood & Cell Culture DNA Kit (QIAGEN ...
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bioRxiv - Bioengineering 2020Quote: ... 1kb homology arms were synthesized by GeneWiz containing regions flanking the cut site (South Plainfield, NJ) and inserted into the donor plasmids using Gibson Assembly Master Mix (New England Biolabs, Inc., Ipswich, MA), with (hmejSRYp ...
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bioRxiv - Neuroscience 2019Quote: ... The cassette was amplified by PCR then ligated to the donor template using Gibson Assembly Master Mix (NEB, primers in Supplementary Table 3). The reaction was incubated at 50 °C for 15 minutes ...
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bioRxiv - Plant Biology 2021Quote: The first round of PCR was performed using 1X Phusion High-Fidelity PCR Master Mix with HF Buffer (New England BioLabs; Ipswitch, MA, USA) with forward primer “reverse transcription primer (RTP)” ...
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bioRxiv - Plant Biology 2021Quote: The first round of PCR was performed using 1X Phusion High-Fidelity PCR Master Mix with HF Buffer (New England BioLabs; Ipswitch, MA, USA) with cDNA diluted 1:10 in water ...
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bioRxiv - Microbiology 2021Quote: ... A 40 bp sequence homology was introduced in the two above mentioned fragments during the PCR and they were ligated with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs; catalog number E5520S). The whole cassette was then amplified with the end primer set ...
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bioRxiv - Neuroscience 2021Quote: ... mCherry-eSlack2 and mCherry-eSlick was assembled and cloned into pOX vector using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs® Inc.), then subcloned into pmaxCloning™ vector (Lonza) ...
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bioRxiv - Biochemistry 2021Quote: ... or pET28a-Hisgp68-(T7pr_gp71-73) plasmids were assembled from two PCR-fragments using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich MA, USA) according to the manufacturer’s protocol.
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bioRxiv - Genetics 2022Quote: ... Both the synthesized DNA fragment and the PCR products were subcloned into PacI- and NotI-digested pBSMVγPDS using NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs, catalog number E2621) following the manufacture’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... The DNA encoding the EGFP-fusion proteins and the P2A peptide was inserted by Gibson assembly using NEBuilder HiFi DNA assembly master mix (New England BioLabs, Ipswich, MA, USA). The HeLa Flp-In T-REx K44A Dynamin2-EGFP-P2A-Caveolin1-mCherry construct was obtained by in vitro mutagenesis of the pcDNA/FRT/TO/Dyn2-EGFP-P2A-Caveolin1-mCherry construct exchanging lysine at position 44 to an alanin using these primers ...
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bioRxiv - Neuroscience 2019Quote: ... and assembled into a pUAST-attB vector (Li et al., 2017) by NEBuilder HiFi DNA assembly master mix (New England Biolabs, Ipswich, MA, USA). A V5 tag was inserted before the stop codon by Q5 site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... using oligonucleotides oUA315 and oUA316 (Table S4) and cloned into a BamHI-linearized pFA6a backbone by NEBuilder DNA Assembly Master Mix (New England BioLabs, Ipswich, MA, USA). The assembly mix was transformed into NEB 10-beta E ...
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bioRxiv - Genomics 2020Quote: ... genomic DNA libraries were constructed for sequencing on Illumina platforms using the NEBNext® DNA Sample Prep Master Mix Set 1 (New England Biolabs, Ipswich, MA). First ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids made in this study were constructed using G-blocks™ and/or PCR products and assembled using NEBuilder® HiFi DNA Assembly Master Mix following manufacturer’s protocol (NEB, Ipswich, MA). Polymerase chain reactions were performed with Q5 DNA Polymerase (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... The DNA encoding EHD2-BFP and the P2A peptide was inserted by Gibson assembly using NEBuilder HiFi DNA assembly master mix (New England BioLabs, Ipswich, MA, USA). The Flp-In TRex HeLa cell lines were maintained in DMEM supplemented with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... Expression constructs were Gibson assembled using GeneBlocks (Integrated DNA Technologies, Coralvile, IA, USA) and NEBuilder HiFi DNA Assembly Master Mix (NEB, Ipswich, MA, USA) to integrate sequences into a vector appropriate for transient expression in CHO cells ...
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bioRxiv - Genetics 2019Quote: ... These cDNA templates were amplified through 23-30 cycles of PCR using Phusion High-Fidelity master mix (New England Biolabs, Ipswich, MA, USA). For MHC class I sequencing ...
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bioRxiv - Molecular Biology 2022Quote: A total of 50ng cleaned-up PCR product was repaired using NEBNext Ultra II End repair/dA-tailing reagent and barcoded using NEBNext Ultra II Ligation Master Mix (New England Biolabs Inc. Massachusets, USA), following manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2022Quote: ... all PCR products were assembled into the pSK275 backbone using the NEBuilder HiFi DNA assembly Master Mix (New England Biolabs, Ipswich, MA, USA), and the constructs were propagated in E ...
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bioRxiv - Genomics 2022Quote: The linear vector used in the ATAC-STARR-seq gibson cloning step was generated by a single 50μL PCR reaction using NEBNext® Ultra™ II Q5® Master Mix (NEB, #M0544S). While not necessary for this study ...
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bioRxiv - Plant Biology 2022Quote: All PCR reactions were performed using the Q5 High-Fidelity DNA Polymerase and the ligation reactions with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs Inc., Ipswich, MA). To generate the binary vector for making MED14 transgenic lines ...
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bioRxiv - Systems Biology 2023Quote: ... PCR with custom Nextera PCR primers was then performed for five cycles using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs, Ipswich, MA, USA) 76 ...
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bioRxiv - Microbiology 2024Quote: ... Purified amplicons were cloned into STARR-seq luciferase reporter vector within the XbaI and FseI sites using Gibson assembly master mix (NEB Cat. No. E5510). The STARR-seq luciferase reporter (vector_ORI_empty ...
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bioRxiv - Immunology 2024Quote: ... The 16S rRNA gene spanning variable regions V3+V4 was amplified using the broad-range forward primer 341F: CCTAYGGGRBGCASCAG and the reverse primer 806R: GGACTACNNGGGTATCTAAT using the Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Beverley, MA). The PCR amplification program consisted of (1 ...
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HTLV-1 Splice Sites in Prevalent Gene Vectors Cause Splicing Perturbations in Transgenic Human CellsbioRxiv - Genomics 2023Quote: ... Following magnetic bead purification one third of the cDNA per sample was subjected to PCR amplifications with the primers HTLV-1 and PE-2nd-GA using the NEBNext® Ultra™ II Q5® Master Mix (NEB) using the cycling program ...
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bioRxiv - Genomics 2023Quote: ... Amplification of the libraries was performed for 13 PCR cycles using the Phusion High-Fidelity PCR Master Mix (New England Biolabs, cat. no. M0531L); 6-bp molecular barcodes were also incorporated during this PCR amplification ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA fragments encoding enriched peptide genes were mixed with sfGFP gene portion and assembled by 1 hour incubation at 50°C using HiFi Master mix (NEB Japan, Tokyo, Japan). The reaction mixture was then used in PCR amplification with primers 13 and 14 to obtain peptide-sfGFP gene fragments suitable for cell-free protein synthesis ...
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bioRxiv - Molecular Biology 2023Quote: ... and produced by PCR amplification (10–13 cycles) of tagmented DNA using a NEB Next High-Fidelity 2× PCR Master Mix (New England Biolabs, Ipswich, MA, USA). DNA fragments were then purified using the MinElute PCR Purification Kit and eluted in 10 µL elution buffer ...
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bioRxiv - Genetics 2023Quote: ... 100 pmol of template oligos and 125 pmol IRDye 700-labeled reverse complement primer F1 were mixed in Phusion® High-Fidelity PCR Master Mix (NEB, Ipswich, MA). A 15s denaturing at 95°C following a 10-minute extension at 52°C afforded duplex DNAs ...
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bioRxiv - Genomics 2023Quote: ... We then ligated barcodes to the end-prepped DNA using the Native Barcoding Expansion 1–12 kit (EXP-NBD104, Oxford Nanopore Technologies, Oxford, UK) and Blunt/TA Ligase Master Mix (New England Biolabs, Ipswich, MA, USA). We cleaned the barcoded samples using 0.9X AMPure XP beads ...
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bioRxiv - Cancer Biology 2024Quote: ... The assembly of these components and subsequent purification steps were carried out through Gibson assembly (Gibson Assembly® Master Mix, NEB, Ref. E2611L). Six electroporations of the bead-purified ligations were performed into NEB10-beta E.coli cells (NEB® 10-beta Electrocompetent E ...
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bioRxiv - Microbiology 2024Quote: ... a 20uL 16S-V4 PCR reaction was set up (1ng extracted gDNA; 1uL forward barcoded P5 primer; 1uL reverse barcoded P7 primer; 10uL NEBNext® Ultra™ II Q5® Master Mix [NEB M0544X] ...
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2-specific amplicons were generated using xGen Artic V4 NCoV-2019 primers (Integrated DNA Technologies Inc., Coralville, Iowa) and Q5 Hot Start High Fidelity PCR Master Mix (New England Biolabs, Cat. no. E0555S). Sequencing libraries were prepared using NEBNext ultra II DNA library kit (New England Biolabs ...