Labshake search
Citations for New England Biolabs :
3501 - 3550 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... a 20uL 16S-V4 PCR reaction was set up (1ng extracted gDNA; 1uL forward barcoded P5 primer; 1uL reverse barcoded P7 primer; 10uL NEBNext® Ultra™ II Q5® Master Mix [NEB M0544X] ...
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2-specific amplicons were generated using xGen Artic V4 NCoV-2019 primers (Integrated DNA Technologies Inc., Coralville, Iowa) and Q5 Hot Start High Fidelity PCR Master Mix (New England Biolabs, Cat. no. E0555S). Sequencing libraries were prepared using NEBNext ultra II DNA library kit (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... The assembly of these components and subsequent purification steps were carried out through Gibson assembly (Gibson Assembly® Master Mix, NEB, Ref. E2611L). Six electroporations of the bead-purified ligations were performed into NEB10-beta E.coli cells (NEB® 10-beta Electrocompetent E ...
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bioRxiv - Immunology 2024Quote: ... were cloned into a pAAV vector using Gibson assembly technique (Gibson et al., 2009) with the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, cat. E2621L). AAVs carrying each HDRT were produced as previously described (Hartweger et al. ...
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bioRxiv - Microbiology 2022Quote: ... and qPCR (E7630C, NEB) to confirm template size and concentration ...
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bioRxiv - Genetics 2022Quote: ... qPCR reactions were conducted using Luna Universal Probe qPCR mastermix (New England Biolabs). Reactions were set up in a total volume of 10 ml containing 0.4 μM of each probe ...
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bioRxiv - Microbiology 2021Quote: ... The RT-qPCRs with Luna Universal One-Step RT-qPCR kit (NEB, USA) were performed according to the protocol of the manufacturer ...
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bioRxiv - Genomics 2022Quote: ... RT-qPCR was performed using Luna Universal One-Step RT-qPCR kit (NEB) using the following primers ...
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bioRxiv - Genomics 2019Quote: ... 10μl of OneTaq HS Quick-Load 2x (NEB, #M0486L), 1μl i5 primer 1uM ...
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bioRxiv - Neuroscience 2020Quote: ... 10µl of OneTaq HS Quick-Load 2x (NEB, #M0486L), 1µl i5 primer 1uM ...
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bioRxiv - Molecular Biology 2024Quote: ... reactions were denatured with 2X RNA dye (NEB B0363S) at 95°C for 10 min ...
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bioRxiv - Genetics 2023Quote: ... 5μl 2X T4 ligase buffer (New England Biolabs, B0202S) and 1μl T4 DNA ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were stopped by adding 2X loading dye (NEB) with EDTA or 6X loading dye (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were stopped by adding 2X loading dye (NEB) with EDTA and running 10% TBE polyacrylamide gels ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were stopped by adding 2X loading dye (NEB) with EDTA and running 10% TBE polyacrylamide gels ...
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bioRxiv - Biochemistry 2023Quote: ... TrpB variants were amplified using Q5 2x MM (NEB): 98°C for 2 minutes ...
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bioRxiv - Microbiology 2024Quote: ... after resuspending in a 2X RNA Loading Dye (NEB). Migration was performed in a mixture of 1X TAE at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The antibody expression construct was built using GeneBlocks (Integrated DNA Technologies, Coralville, IA, USA) and NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs, Ipswich, MA, USA). The construct was amplified in NEB 10-beta E ...
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bioRxiv - Molecular Biology 2021Quote: Pooled sgRNA oligonucleotides were synthesized as 76-mers by Custom Array (Bothell, WA, USA) and were amplified by PCR with NEBNext High Fidelity PCR Master Mix (New England BioLabs, NEB, Ipswich, MA, USA) using customized primers (Additional file 2 ...
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bioRxiv - Microbiology 2021Quote: ... Barcodes were ligated to DNAs for each of the four tick samples using Blunt/TA Ligase Master Mix (New England Biolabs Inc., Ipswich, United States) and the EXP-NBD104 Native Barcoding Expansion Kit (ONT) ...
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bioRxiv - Cancer Biology 2021Quote: ... The libraries were then prepared using NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina (#E6240, New England Biolabs, Ipswich, Massachusetts, USA) and sequenced on an Illumina HiSeq 2000 sequencer (RRID:SCR_020132 ...
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bioRxiv - Immunology 2022Quote: ... the internal primers contain complementary 30 nucleotides of the respective expression vectors in order to be ligated by Gibson reaction using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, catalog no. E2621L). Ligated products were transformed into One Shot Stbl3 competent E ...
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bioRxiv - Genetics 2021Quote: ... The col-10p::TIR1 line was generated by cloning to combine the col-10 promoter with the TIR1 construct of pDD356 (NEB Hifi Assembly Master Mix). Plasmid pAP082 was used to express Cas9 and a sgRNA to target the chromosome I insertion site ...
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bioRxiv - Microbiology 2021Quote: ... The PCR amplification was carried out with Phusion High-Fidelity PCR Master Mix with HF Buffer in 50uL reactions with 20ng of genomic DNA (New England Biolabs Inc., Ipswich, MA, USA). Reactions were run in an Agilent SureCycler 8800 (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... The DAP gDNA library was prepared using the kit from NEBNext® DNA Library Prep Master Mix Set for Illumina® (NEB #E6040S/L). ZmIBH1-1 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat#G184A ...
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bioRxiv - Cell Biology 2022Quote: ... with primer-OCY581-NGS-Lib-KO-Fwd-first-step and OCY582-NGS-Lib-KO-Rev-first-step was performed using NEBNext® Ultra™ II Q5® Master Mix (New England Biolabs, M0544L). The first-step PCR products were recovered with QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... 5’-GCT GCG TTC TTC ATC GAT GC-3’) were chosen with barcodes for PCR amplification using Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA, USA). The PCR products were then mixed at equal density ratios and purified with a Gel extraction kit (QIAGEN ...
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bioRxiv - Genomics 2023Quote: ... The double-stranded lenti_epegRNA oligos were then assembled with the linearized Lenti-epeg-Puro-P2A-EGFP backbone using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, Cat. No. E2621S) using the standard protocol ...
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bioRxiv - Genomics 2023Quote: ... These two amplification products were then assembled into a single vector (PB-EFS-PEmax) using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, Cat. No. E2621S) using the standard protocol for a 2-3 fragment assembly ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was converted into double stranded blunt-end libraries with Illumina-specific adapters REF using the NEBNext DNA Sample Prep Master Mix Set 2 (E6070S - New England Biolabs Inc., Beverly, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... All 5μl of the cell lysis were used for each Genomic PCR amplification using NEBNext® Ultra™ II Q5® Master Mix (NEB, M0544S). PCR program is 1 cycle ...
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bioRxiv - Microbiology 2019Quote: ... 4 μl of Low Range ssRNA ladder (NEB) or 5 μl RNA molecular weight marker I DIG-labelled (Roche ...
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bioRxiv - Synthetic Biology 2020Quote: ... Low Molecular Weight DNA Ladder (New England Biolabs), ssRNA ladder and Low range ssRNA ladder standards (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... and low range ssRNA ladder (New England Biolabs) were used as size markers ...
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bioRxiv - Cancer Biology 2023Quote: ... A low molecular weight ladder (NEB cat. #: N3232L) and high molecular weight ladder (NEB cat ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed using the Luna Universal One-Step RT-qPCR Kit (NEB) in an Applied Biosystems QuantStudio 3 thermocycler ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using the Luna Universal One-Step RT-qPCR Kit (NEB) in an Applied Biosystems QuantStudio 7 thermo-cycler ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was performed using Luna Universal One-Step RT-qPCR reagents (NEB, E3005) and the primers listed in Table S3.
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bioRxiv - Genomics 2022Quote: ... RT-qPCR was performed with Luna Universal One-Step RT-qPCR Kit from NEB on BioRad CFX96 Touch Real-Time PCR Detection System ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed using the LUNA 1-step RT-qPCR kit (E3005L, NEB) on an AriaMx Real-time PCR system (G8830A ...
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bioRxiv - Developmental Biology 2020Quote: ... The amplification of V3-V4 hypervariable region of the 16S rRNA gene was carried out with formwork of each of the DNA samples using the primer set 515F/806R and Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA, United States) as described by Caporaso et al ...
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bioRxiv - Systems Biology 2022Quote: ... custom-designed PCR primers that contain dual-indexed adapters were used to amplify the V3-V4 (806R/319F) region using NEBNext® Q5® Hot Start HiFi PCR Master Mix (New England Biolabs, Beverly, MA, USA). Samples were purified by two repeated 0.8X-ratio magnetic bead clean ups to remove all traces of primer ...
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bioRxiv - Genomics 2024Quote: All adapter-ligated amplicons were amplified with custom iTru5 and iTru7 index primers (Glenn et al. 2019) with NEBNext Ultra II Q5 Master Mix (NEB, Cat No, M0544, Ipswich, MA) according to reaction conditions described in Supplemental Table 6 ...
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bioRxiv - Molecular Biology 2020Quote: ... and qPCR analysis by NEB Next Library Quant Kit for Illumina (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... The Luna qPCR (NEB #M3004) was used to quantify the generated cDNA ...
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bioRxiv - Genomics 2023Quote: ... and ran qPCR (NEB, M3003L) in triplicates ...
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bioRxiv - Genomics 2024Quote: ... Samples were then rinsed with 2X SSC before incubating with a blocking buffer (10% BSA, 3% v/v 6% v/v murine RNase inhibitor [NEB, M0314L] in 2X SSC) for 30 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μl of 2X RNA loading dye (New England Biolabs) was added ...
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bioRxiv - Molecular Biology 2022Quote: ... plus 15µL NEBNext 2x HiFi PCR MasterMix (New England Biolabs) and thermocycling with the following conditions ...
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bioRxiv - Synthetic Biology 2022Quote: ... After the addition of the RNA loading dye 2x (NEB) and the heat denaturation at 65 °C for 5 min ...