Labshake search
Citations for New England Biolabs :
3201 - 3250 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... Both the insert and vector PCR reactions were then column-purified and we assembled the vector and promoter variants using Gibson assembly (Gibson et al. 2009) with NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). The assembly mix was then electroporated into the electrocompetent MG1655 strain ...
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bioRxiv - Molecular Biology 2022Quote: ... Tagmented DNA was PCR amplified using custom i5 and i7 PCR primers and Phusion® High-Fidelity PCR Master Mix with GC Buffer (NEB). PCR conditions were ...
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bioRxiv - Genomics 2022Quote: ... we performed two separate 100 ul reactions (25 cycles each) with 250 ng of input gDNA using Q5 MASTER MIX (NEB #M0491) and the resulting products were pooled (PCR reaction ...
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bioRxiv - Microbiology 2019Quote: Bacterial 16S rRNA genes were amplified from 50 ng DNA per sample diluted to 10 µL nuclease free water in 50 µL with 1 × LongAmp Taq master mix (New England Biolabs, Inc), 1 µL 16S rRNA gene barcoded primer (ONT-SQK-RAB-201 ...
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bioRxiv - Cell Biology 2019Quote: ... The decoupling and control construct were assembled via Gibson cloning using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). The decoupling construct contained the C-terminal end of the nesprin-3 gene (Syne3 ...
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bioRxiv - Molecular Biology 2019Quote: ... Master mix was added to each sample together with 1 µl truncated T4 RNA Ligase 2 (#M0239, NEB; Frankfurt/Main, Germany). Ligation was carried out for 2 h at 23°C and nucleic acids were precipitated ...
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bioRxiv - Molecular Biology 2019Quote: ... The gel-purified product was cloned into the NcoI and BamHI sites in the expression vector pET15b by using the NEBuilder® HiFi DNA Assembly Master Mix (E262, NEB) and the appropriate primers ...
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bioRxiv - Genomics 2019Quote: ... were PCR-amplified (2 PCR reactions, 12 cycles) using Illumina adapter-specific primers and NEBNext® Ultra II Q5 Master Mix (NEB). After library profile analysis by Agilent 2100 Bioanalyser (Agilent Technologies ...
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bioRxiv - Synthetic Biology 2019Quote: The adipic acid biosensing plasmid (JBx_101898) was assembled of 4 DNA parts by NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, MA): First ...
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bioRxiv - Bioengineering 2020Quote: ... and cloned into pET vector in frame with a C-terminal 6XHis tag by Gibson assembly (NEBuilder® HiFi DNA Assembly Master Mix, New England Biolabs). DNA encoding SARS-CoV-2 S RBD (S a.a ...
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bioRxiv - Pathology 2019Quote: ... protocol using the PCR Barcoding Expansion 1-12 (EXP-PBC001) kit (ONT) and LongAmp Taq 2× Master Mix (New England Biolabs, MA) with the following thermocycling conditions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid backbone and the PCR product was gel purified and ligated via Gibson assembly using NEBuilder HiFi DNA assembly master mix as per manufacturer’s instructions (New England BioLabs; Ipswich, MA). Plasmids were transfected into NEB5α bacteria and positive clones screened by PCR and verified by Sanger sequencing.
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bioRxiv - Genetics 2020Quote: ... and cloned it into a lentiviral transfer pLEX vector carrying the hygromycin resistance gene using Gibson Assembly Master Mix (NEB E2611L). A 2A epitope tag was added to hACE2 at the C-terminus ...
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bioRxiv - Synthetic Biology 2020Quote: ... Peptide tags were cloned into pFA6a-KANMX6 by amplifying the plasmid backbone and inserting gBlocks (Integrated DNA Technologies) using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). Except where otherwise noted ...
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bioRxiv - Microbiology 2019Quote: ... All plasmids were constructed via Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Inc., Cat. No. E2621X) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... was linerized by inverse PCR with primers 13 and 14 and the duplex oligo was ligated to the linearized plasmid in a 2:1 insert:vector molar ratio using NEBuilder Hifi Assembly Master Mix (New England Biolabs, Ipswich, Massachusetts) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... purified and an indexed sequencing library was prepared using the NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina in combination with NEBNext® Multiplex Oligos for Illumina (New England Biolabs). The quality of sequencing libraries was verified on a Bioanalyzer DNA High Sensitivity chip (Agilent ...
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bioRxiv - Cancer Biology 2020Quote: ... The sequencing libraries were constructed by adding 3’ adenylation and ligating barcoded sequencing adaptors to the fragmented DNA using NEBNext DNA Library Prep Master Mix set for Illumina (NEB, E5040L). The generation of barcoded sequencing adapters can be found in 59 ...
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bioRxiv - Genomics 2021Quote: The HA-AID-CTCFcDNA-AID-eGFP-blasticidin vector was assembled by Gibson Assembly (NEBuilder HiFi DNA Assembly Master Mix, NEB, E2621L) in the pENTR221 kanamycin vector using the following templates ...
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bioRxiv - Genomics 2021Quote: The AID C-terminal RPB1-AID-eGFP-blasticidin vector was assembled by Gibson Assembly (NEBuilder HiFi DNA Assembly Master Mix, NEB, E2621L) in the pENTR221 kanamycin vector using the following templates ...
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bioRxiv - Genomics 2021Quote: ... and introduced between XhoI and BamHI sites in c-Flag pcDNA3 (addgene #20011) to generate Flag-tagged hPEX5 using NEBuilder® HiFi DNA assembly Master mix (New England Biolabs). Site-directed mutagenesis for amino acid substitution was performed using the Q5® Site-directed mutagenesis kit (New England Biolabs ...
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CRISpy-pop: a web tool for designing CRISPR/Cas9-driven genetic modifications in diverse populationsbioRxiv - Genetics 2020Quote: ... was first cloned into the pKOPIS plasmid (Kuang et al. 2018) using the NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) (Hsieh 2018) ...
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bioRxiv - Genetics 2021Quote: ... 200-800 nt amplicons were amplified from genomic DNA from individual insertion lines through single fly PCR (Gloor et al., 1993) using OneTaq PCR master mix (NEB #M0271L). PCR conditions were 95°C for 30 seconds ...
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bioRxiv - Genomics 2020Quote: ... 1 μM of NEBNext Unique Dual Index Primers and 25 μl NEBNext Q5U Master Mix (M0597, New England Biolabs, Ipswich, MA) were added to the DNA and amplified as follows ...
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bioRxiv - Biochemistry 2021Quote: ... was fused on a pBAD33 arabinose inducible expression vector (Aigner and Wilson, 2017) using Gibson Assembly strategy (Gibson et al., 2009) with Gibson Assembly® Master Mix from NEB. Primer sets used for assembly were listed in Table S5 ...
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bioRxiv - Microbiology 2021Quote: ... DNA fragments located upstream and downstream to the epitope insertion sites were amplified by PCR using the Q5® High-Fidelity 2 × Master Mix (NEB) with relevant primers (Table 1 ...
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bioRxiv - Immunology 2020Quote: ... First-round Ig-encoding sequence amplification (20 cycles) was performed using Q5 High-Fidelity Master Mix (New England BioLabs, Ipswich, MA) and nested gene-specific primers ...
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bioRxiv - Immunology 2020Quote: ... separate HDR-donor VL libraries were assembled in a stepwise manner by Gibson cloning using the Gibson Assembly Master Mix (NEB, E2611)33 ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR inserts were assembled with restriction enzyme digested pmCherry-N1 backbone in Gibson Assembly Master Mix (New England Biolabs; E2611S) as per manufacturer recommendations and the assembled plasmids transformed into NEB5a cells (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... and used for PCR amplification of the target region using the Q5® Hot Start High-Fidelity 2× Master Mix (NEB), followed by evaluation of the PCR products by gel electrophoresis and purification with the Qiaquick PCR purification kit (28104 ...
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bioRxiv - Immunology 2021Quote: ... and after hybridization were cloned into Esp3I-digested lentiGuide-puro using the NEBuilder® HiFi DNA Assembly Master mix (E2621, NEB). Successful constructs were verified in plasmid from isolated colonies by sequencing with the LKO.1 5’ primer ...
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bioRxiv - Genomics 2021Quote: ... PCR amplification of the samples was performed using Nextera primers 1 and 2 and NEBNext High fidelity master mix (NEB, M0541S) for 12 cycles as determined by KAPA Real-Time Library Amplification Kit (Peqlab ...
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bioRxiv - Genomics 2021Quote: ... 46 and Carøe et al 45 and amplified with Phusion® High-Fidelity PCR Master Mix with HF buffer (New England Biolabs Inc). The appropriate number of cycles were determined using Mx3005 qPCR (Agilent Technologies ...
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bioRxiv - Genomics 2021Quote: ... Produced insert was cloned into the abovementioned pENTR vector from Twist Biosciences bearing GSH1 and GSH2 300bp homology arms as well as CMV promoter sequence using Gibson Assembly Master Mix (NEB, #E2611L). HDR donors were amplified from these plasmids using biotinylated primers with phosphorothioate bonds between the first 5 nucleotides on both 5’ and 3’ ends ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 µl of this DNA mixture and 2.5 µl of UltraPure water was added to 5 µl of NEBuilder® HiFi DNA Assembly Master Mix (NEB E2621) on ice ...
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bioRxiv - Microbiology 2021Quote: ... The promoter sequence and Cas9 expression cassette were assembled into the multiple cloning site of pUC19 using the NEBuilder HIFI DNA Assembly master mix (NEB #E2621) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... All the pieces necessary for genome editing via Homology Direct Repair (HDR) were inserted via NEBuilder HiFi DNA Assembly Master Mix (NEB #2621) into a plasmid with a negative selection marker that can be used to screen against integration of the entire plasmid into the genome ...
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bioRxiv - Cell Biology 2022Quote: All plasmids reported in this work were constructed using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs Cat. #E2621), based on the Gibson assembly method (Gibson et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... Adaptor-ligated DNA fragments of proper size were enriched with PCR reaction using Fusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Immunology 2022Quote: ... and myc genes into a retroviral vector (containing CD19 CAR gene) using NEB HiFi DNA Assembly Master Mix (NEB cat. # E2621S).
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bioRxiv - Immunology 2022Quote: Point mutations were introduced through linearizing the plasmid by PCR with primers containing the intended mutations (Supp. Table S1) followed by reverting to circular plasmid with Gibson Assembly Master Mix (M5510A, NEB, USA).
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bioRxiv - Microbiology 2022Quote: ... Assembly of the two cDNA fragments was done using five overlapping cDNA fragments containing the VOC lineage defining mutations and replicon specific gene replacements (see Supplementary Table 3) using a NEBuilder® HiFi DNA Assembly Master Mix (NEB) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... followed by assembling of the amplified gene via Gibson Assembly according to the manufacturer’s instructions (Gibson Assembly® Master Mix, New England Biolabs, U.S.A.). Identity of the obtained plasmid pEKEx2_e2-crimson was verified via colony PCR.
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bioRxiv - Synthetic Biology 2022Quote: ... DNA fragments to be assembled were amplified by PCR using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532S), purified with gel electrophoresis and Zymoclean Gel DNA Recovery Kit (Zymo Research ...
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bioRxiv - Synthetic Biology 2022Quote: The PCR insert was assembled into 1600 ng of a linearized mRNA selection vector (AAV9-CMV-Express) with NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621L) at a 3:1 insert:vector Molar ratio in a 80 μL reaction volume ...
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bioRxiv - Molecular Biology 2022Quote: ... using the previously published pFF-12 as a template.18 Insert assembly and ligation into PCR-linearized pJAK184 (FFO-362/-363) was achieved via Gibson Assembly (Gibson Assembly ® Master Mix, New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... genes of interest were amplified from phage or bacterial genomic DNA using Q5 Hot Start High Fidelity Master Mix (NEB, M0494L), or synthesized as GeneFragments (Genewiz ...
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bioRxiv - Microbiology 2022Quote: ... Illumina DNA library preparation was performed using NEBNext® DNA Library Prep Master Mix Set for Illumina® (New England Biolabs), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... 100 ng of the amplified tRNA operon was assembled into the linearized pRK2-cat backbone using the HiFi DNA Assembly Master Mix (New England Biolabs, USA). After incubation for 60 mins at 50 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... each sample was combined with 2.5 uL native barcode (Oxford Nanopore Technologies, EXP-NBD104 or EXP-NBD114) and 25 uL of Blunt/TA Ligase Master Mix (NEB, M0367). The ligation reaction was incubated for 10 minutes at room temperature ...