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Citations for New England Biolabs :
3151 - 3200 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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BET protein inhibition regulates macrophage chromatin accessibility and microbiota-dependent colitisbioRxiv - Immunology 2021Quote: ... Transposed DNA samples were purified using the Qiagen MinElute Kit (#28204) followed by amplification using 1x PCR master mix (NEB #M0541S) and 25 μM Ad1_noMX and Ad2.* indexing primer for 10-14 cycles ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was precipitated and resuspended in ligation master mix (1X NEB Ligation buffer, 0.8% Triton X-100, 0.5X BSA, 1600U of T4 DNA ligase (NEB, Catalog number: M0202). The reaction was incubated at 21 °C for 4 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... 948 μL of ligation master mix was then added: 150 μL of 10× NEB T4 DNA ligase buffer with 10 mM ATP (NEB, B0202), 125 μL of 10% Triton X-100 ...
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bioRxiv - Genomics 2019Quote: ... The DNA (50-100 ng) was amplified using the Phusion Hi-Fi PCR master mix with HF buffer (New England Biolabs M0531) or the Q5 Hot Start HiFi PCR master mix (New England Biolabs E6625AA ...
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bioRxiv - Synthetic Biology 2020Quote: All single gRNA-expressing plasmids were assembled by Gibson assembly reaction using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). Depending of the plasmid features ...
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bioRxiv - Cell Biology 2019Quote: ... red fluorescent protein coding sequence (dsRed) were cloned into the above derivative tetracycline inducible plasmid with NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs) at NheI and MluI restriction sites (pLVD-GRASLND and pLVD-dsRed) ...
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bioRxiv - Cell Biology 2019Quote: A derivative vector from modified TMPrtTA (3, 70) was created with NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). Backbone was digested with EcoRV-HF (New England Biolabs ...
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bioRxiv - Developmental Biology 2019Quote: ... One replicate of each ChIP-seq library was prepared with NEBNext ChIP-seq Library Prep Master Mix Set (NEB, Cat#E6240S) using insert size selection of 250 bp ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were cloned into NarI and AvrII cut WT DENV-Luc infectious clone using NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs) and transformed into Stbl3 bacteria (Berkeley MacroLabs) ...
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bioRxiv - Microbiology 2019Quote: ... All fragments contained ~20bp overhangs and were assembled into EcoRV cut pLenti CMV Puro DEST (w118-1) using NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs) or Gibson-like master mix (Berkeley MacroLabs ...
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bioRxiv - Genomics 2019Quote: ... 948 µL of ligation master mix was then added: 150 µL of 10× NEB T4 DNA ligase buffer with 10 mM ATP (NEB, B0202), 125 µL of 10% Triton X-100 ...
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bioRxiv - Molecular Biology 2019Quote: ... 948 µL of ligation master mix was then added: 150 µL of 10x NEB T4 DNA ligase buffer with 10 mM ATP (NEB, B0202), 125 µL of 10% Triton X-100 ...
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bioRxiv - Genetics 2020Quote: ... 50 pg of repaired and adapter-ligated products were amplified for 19 cycles with the NEBNext Ultra II Q5 Master Mix (New England Biolabs M0544) and dual-indexed with custom IDT Ultramer primers ...
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bioRxiv - Genomics 2021Quote: ... standard Gibson Cloning for S1 or S2 inserts plus PCR-amplified/DpnI-digested backbone was performed using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs) at 3:1 insert to vector ratio ...
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bioRxiv - Genomics 2021Quote: ... for 30 min at 37 °C and PCR amplification to add the Illumina smallRNA barcodes and handles by adding 25 μl of NEBNext Ultra II Q5 Master Mix (NEB, M0492L), 11 μl Nuclease free water and 2 μl of RP1 and RPIx primers (10 μM) ...
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bioRxiv - Microbiology 2021Quote: ... Gibson assembly was performed to ligate DNA fragments with their corresponding digested vectors using the NEBuilder® HiFi DNA Assembly Master Mix (NEB) according to the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2021Quote: ... homology arms were amplified from genomic DNA and introduced to the MCS of the linearized SIBR-Cas plasmid using the NEBuilder® HiFi DNA Assembly Master Mix (NEB). The plasmid was linearized using Esp3I (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... The amplification of bacterial DNA was achieved by targeting the V3–V4 region of 16S rRNA gene with 30 µL final volume containing 15 µL of 2× master mix (BioLabs, USA), 3 µL of template DNA ...
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bioRxiv - Cell Biology 2021Quote: ... CK1γ3 kinase dead mutants were generated using PCR mutagenesis and cloned into pDONR223 digested with BsrGI using Gibson Assembly Master Mix (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cell Biology 2021Quote: ... together with the sequence of SBP-mCherry using seamless cloning with the NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs, E2621). To construct the truncated fragments ...
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bioRxiv - Synthetic Biology 2021Quote: ... The plasmid of pAC-BETAipi-ispA/dxs was constructed by using Gibson assembly kit (Gibson Assembly® Master Mix, New England Biolabs). The insert was amplified from genomic DNA of E ...
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bioRxiv - Microbiology 2020Quote: ... Fragments were amplified by PCR (primers used in this study are shown in Table S6) then ligated into pUC19 (Yanisch-Perron et al., 1985) using NEBuilder® HiFi master mix (New England Biolabs). Resulting plasmids were transformed into E ...
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bioRxiv - Plant Biology 2021Quote: ... A 2.3 kb amplicon including the NAC18.1 gene and ∼800 bp of upstream sequence was amplified by PCR using primers NAC18F2 and NAC18R2 (Table S1) and Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB). PCR product size and purity was confirmed by agarose gel electrophoresis ...
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bioRxiv - Immunology 2020Quote: ... PCRs were pooled by germline and assembled sequentially into the phagemid using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). Transformations were performed using One Shot™ TOP10 or E ...
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bioRxiv - Microbiology 2021Quote: ... the sgRNA-encoding region was amplified from 500 ng genomic DNA with 17 cycles of PCR using NEBNext Ultra II Q5 master Mix (NEB #M0544L). Each PCR reaction contained a pool of forward primers (0.5 μM final concentration ...
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bioRxiv - Microbiology 2021Quote: ... The flanking regions were amplified from the Synechocystis genome through PCR and assembled with an aadA resistance cassette into a pRL271-based vector backbone by Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, USA). Transformants were streaked on BG-11 agar plates with increasing concentrations of spectinomycin up to 25 µg ml-1 and full segregation was tested by colony PCR ...
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bioRxiv - Cell Biology 2021Quote: ... pYX142 and pNH605 plasmids used in this study was performed by Gibson Assembly with the HiFi DNA Assembly Master Mix (New England Biolabs, #E2621L) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... SecM17 and NanoLuc sequences were synthesized (Integrated DNA Technologies) and inserted into the open reading frame with Gibson Assembly Master Mix (NEB E2611). All commercially available enzymes were purchased from NEB unless stated otherwise ...
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bioRxiv - Cell Biology 2021Quote: ... ChIP libraries were prepared for Illumina NextSeq 500 using NEBNext ChIP-Seq DNA Sample Prep Master Mix Set for Illumina (NEB E6240) and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 ...
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bioRxiv - Genomics 2020Quote: ... A 2µ -URA plasmid was constructed from three DNA fragments using HiFi DNA assembly Master Mix (New England Biolabs, Ipswich, MA) for cloning ...
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bioRxiv - Genomics 2022Quote: ... Illumina libraries and DNA splint were mixed at a 1:1 ng ratio using NEBuilder HiFi DNA assembly Master mix (NEB #E2621). Any non-circularized DNA was digested overnight using ExoI ...
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bioRxiv - Molecular Biology 2022Quote: The nCas9-RT fragment from pCMV-PE2 was retrieved by BglII digestion then cloned into the pET30a(+) expression vector in frame between the NotI and NdeI sites using NEBuilder® HiFi DNA Assembly Master Mix (NEB) with bridging gblocks (Supplemental Table 1 ...
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bioRxiv - Cancer Biology 2022Quote: All cloning was performed by PCR and DNA assembly using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs #E2621). Constructs for transient expression were cloned into pEGFP-C1 backbones encoding a CMV promoter ...
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bioRxiv - Genomics 2022Quote: ... The Hi-TrAC libraries were then amplified with multiplexing indexed primers in the following reaction mixture: 20 μL Phusion HF PCR Master Mix (NEB, M0531S), 1 μL Illumina Multiplexing PCR primer 1.0 (10 μM) ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA fragments to be assembled were amplified by PCR using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532S), purified with gel electrophoresis and Zymo clean Gel DNA Recovery Kit (Zymo Research,D4002) ...
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bioRxiv - Biochemistry 2022Quote: ... Linearised plasmid was mixed with 2-3-fold excess of the three insert fragments followed by addition of Gibson Assembly® Master Mix (New England Biolabs) and incubation at 50°C for 60 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... The sequence was amplified from 50-100 ng of genomic DNA using Phusion High-Fidelity DNA Polymerase master mix PCR protocol (NEB #M0530). Gel purified PCR amplicons were sent to Integrated Genomic Operation (IGO ...
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bioRxiv - Synthetic Biology 2022Quote: ... and assembled by Gibson Assembly (Gibson et al., 2009) reaction according to the manufacturer’s instructions (Gibson Assembly Master Mix, New England Biolabs, Ipswich, USA). Two microliters of the assembly reaction were taken for the consequent bacterial transformation.
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bioRxiv - Microbiology 2022Quote: ... the primer sets amplifying sar5 and the pKT1 vector backbone were extended with overlaps to enable Gibson Assembly with the Gibson Assembly® Master Mix (NEB). KOD Xtreme™ Hot Start DNA Polymerase (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... and amplicons were generated by PCR (primers 341F and 806R) using a Phusion® High-Fidelity PCR Master Mix (New England Biolabs). Amplification product quality was assessed by gel electrophoreses and samples were pooled in equimolar ratios ...
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bioRxiv - Microbiology 2022Quote: ... The sgRNA-encoding region was amplified from 500ng genomic DNA with 17 cycles of PCR using NEBNext Ultra II Q5 master mix (NEB M0544L) as described in ...
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bioRxiv - Microbiology 2020Quote: ... faecalis ΔessB was generated by allelic replacement by cloning an in frame essB deletion product into pLT06 using Gibson Assembly® Master Mix (New England Biolabs), integrating this construct into the chromosome ...
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Sterilizing immunity against SARS-CoV-2 in hamsters conferred by a novel recombinant subunit vaccinebioRxiv - Microbiology 2020Quote: ... These target genes were constructed into a modified PiggyBac (PB) transposon vector EIRBsMie using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, E2621L) described previously (44).
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bioRxiv - Microbiology 2021Quote: ... The same plasmid was then digested with AfeI and AflII and assembled with the gel purified PCR products using the HiFi Assembly Master Mix (NEB, E5520).
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bioRxiv - Molecular Biology 2020Quote: Plasmids were constructed using PCR fragments and restriction cloning or Gibson assembly methods using Gibson Assembly Master Mix (New England Biolabs E5510). Plasmids and assembly templates were designed in silico using SnapGene software (GSL Biotech ...
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bioRxiv - Biochemistry 2020Quote: ... and inserted into the EcoRI/NcoI-sites of the bicistronic pRF vector (Yoon et al., 2006) by Gibson assembly using the NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621S). Sequences were based off the ENSEMBL database (Zerbino et al. ...
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bioRxiv - Neuroscience 2022Quote: ... G-blocks for this sequence were then generated by Azenta Life Sciences and were assembled using Gibson Assemble master mix (NEB: E2611) into a mammalian expression vector modified from pCDNA3.1 to contain a T2A self-cleaving peptide followed by an in-frame blue fluorescent protein (BFP ...
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bioRxiv - Neuroscience 2022Quote: ... and a PCR amplicon containing the CRISPRoff-v2.1 protein from Addgene plasmid 167981 (gifted from Luke Gilbert [45]) was inserted into the backbone downstream of the TRE using NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) following the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Filled-in chromatin was then ligated by adding 970 μl of Ligation Master Mix (150 μl of 10x NEB T4 DNA ligase buffer with ATP (NEB, B0202), 125 μl of 10% Triton X-100 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The reaction with randomly mutated promoter variants was DpnI- treated and column-purified before Gibson assembly with the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). The assembly mix was then electroporated into the MG1655 strain and colonies that grew on LB with Kanamycin were picked for Sanger sequencing and stored as glycerol stocks ...