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Citations for New England Biolabs :
3101 - 3150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... the backbone PCR reaction was digested overnight with DpnI (New England Biolabs, Ipswich, MA) at 37°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... The variants were created from the wild type plasmids using the Q5 Site-Directed Mutagenesis Kit (NEB) and transformed into MegaX cells made chemically competent (Mix & Go E ...
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bioRxiv - Synthetic Biology 2024Quote: ... DpnI treated PCR products were purified with silica column (FastGene Gel/PCR Extraction Kit, Nippon Genetics Co., Ltd., Tokyo, Japan) and then HiFi assembly reaction (New England Biolabs, Ipswich, MA) was carried out to connect each end of linear DNA products ...
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bioRxiv - Synthetic Biology 2024Quote: ... Golden Gate Assembly using PaqCI (NEB #R0745) and T4 Ligase (NEB #M0202 ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCRs were performed with Q5 High-fidelity DNA polymerase (NEB, M0491L) plus GC buffer with the following reaction conditions ...
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bioRxiv - Plant Biology 2024Quote: ... The RNA samples were treated with DNase I (NEB, RNase-free, Ipswich, MA, USA) and approximately 1 μg of RNA was reverse transcribed using SuperScriptTM II RT (Invitrogen ...
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bioRxiv - Synthetic Biology 2024Quote: Using the HiScribe T7 High Yield RNA Synthesis Kit (NEB) 1 µg of the amplified and linearized DNA construct was transcribed in an 80 µL reaction volume and incubated overnight at 37 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Titers were determined by quantitative PCR on DNase I (NEB) treated and proteinase K (Qiagen ...
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bioRxiv - Synthetic Biology 2024Quote: ... and ligated into the previously digested backbone plasmid using a T4 ligase (NEB; 30 min, 37 °C). For each library candidate ...
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bioRxiv - Synthetic Biology 2024Quote: ... the ColE1 ORI was replaced with the R6Kγ ORI using NEBuilder HiFi DNA Assembly (New England Biolabs). The R6Kγ ORI was amplified from pBAM142 ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli (New England Biolabs, C3020K) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... 20 s annealing at 63 °C and 30 s elongation by Q5 High-Fidelity DNA Polymerase (New England Biolabs) at 72 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli (New England Biolabs, C3020K) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: Standard Q5 high-fidelity DNA polymerase (NEB) PCR conditions and protocols were used for the DNA amplification ...
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bioRxiv - Synthetic Biology 2024Quote: Gibson assemblies of each promoter library were executed using NEBuilder Hifi Assembly Master Mix (New England Biolabs, Ipswich, MA) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR assays were performed using the Luna Universal qPCR Master Mix (NEB) in a MasterCycler RealPlex 4 (Eppendorf) ...
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bioRxiv - Synthetic Biology 2024Quote: gRNA plasmids were generated by T4 PNK phosphorylating (NEB) and annealing two oligos ...
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bioRxiv - Synthetic Biology 2024Quote: ... and all PCR reactions were set up with Q5 Master Mix (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2024Quote: ... Enzymes used for plasmid construction were purchased from NEB. Primers were obtained from IDT and Eurofins genomics ...
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bioRxiv - Neuroscience 2024Quote: ... and OneTaq DNA polymerase mastermix (NEB, M0488L). PCR products were separated by agarose gel electrophoresis using 1.5% agarose (VWR ...
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bioRxiv - Systems Biology 2024Quote: ... at 16 °C overnight, followed by heat-inactivation (65°C, 15 min) and linearization using SbfI-HF (NEB) at 37°C for 2 h ...
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bioRxiv - Systems Biology 2024Quote: ... Libraries were generated from 1 μg DNA per sample using the NEBNext Ultra DNA Library Prep Kit (NEB #E7645, USA) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... first a highly active HIS4-targeting sgRNA was cloned into D-227 vector by digesting the plasmid with BbVCI enzyme (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... The digests were then purified using a Monarch PCR&DNA Cleanup Kit (NEB) and amplified using firstly a nested PCR reaction with KAPA HiFi HotStart ReadyMix (Roche) ...
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bioRxiv - Systems Biology 2024Quote: ... mRNA was then purified using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... the RNA was purified with the Monarch RNA Cleanup Kit (NEB, Cat. #T2050) and run on a 1.5% agarose gel with 0.3% hydrogen peroxide (Supplementary Figure S7a)45 ...
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bioRxiv - Systems Biology 2024Quote: ... Samples were then treated with 4 units of DNase I (NEB) for 1 hr at 37°C ...
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bioRxiv - Systems Biology 2024Quote: ... Libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit (NEB, E7420) and PCR amplified (15 min at 37°C ...
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bioRxiv - Systems Biology 2024Quote: ... using the NEBuilder Hi-Fi Assembly kit (New England Biolabs). To ensure representation of all variants in the population ...
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bioRxiv - Microbiology 2024Quote: ... Phusion polymerase (NEB) was used to amplify the DNA sequence (Table S4) ...
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bioRxiv - Systems Biology 2024Quote: ... Low complexity regions of JNK2α2 and ASK1 were deleted using Q5 Site-Directed Mutagenesis (NEB). Oligonucleotides used for cloning and site-directed mutagenesis are listed in the Table S2.
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bioRxiv - Systems Biology 2024Quote: ... Genomic DNA was extracted by Monarch Genomics DNA Preparation Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... 0.2 μL of Hot Start Taq DNA Polymerase (NEB), 4 μL of Primer Forward (2.5 μM) ...
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bioRxiv - Systems Biology 2024Quote: ... Plasmids were digested with restriction enzymes from New England Biolabs (NEB, Ipswich, MA). Ligations used T4 DNA ligase (NEB M0202 ...
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bioRxiv - Systems Biology 2024Quote: ... 1 µg gDNA was digested with 4U MmeI (NEB) in a 50 µl reaction at 37°C ...
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bioRxiv - Systems Biology 2024Quote: ... 2 μL of 10x Standard Taq Reaction Buffer (NEB), 0.4 μL of dNTPs (2.5 μM) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR protocol involved two amplification steps using New England Biolabs (NEB, Ipswich, MA, USA) reagents ...
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bioRxiv - Synthetic Biology 2024Quote: ... and Q5 Polymerase from NEB, followed by gel purification ...
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bioRxiv - Synthetic Biology 2024Quote: Cloning was performed using restriction enzyme digestion and ligation with T4 ligase (New England Biolabs) or Gibson assembly (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2024Quote: ... T4 DNA Ligase (NEB #M0202L), Antarctic Phosphatase (NEB #M0289L ...
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bioRxiv - Synthetic Biology 2024Quote: ... NEBridge Golden Gate Assembly BsaI-HFv2 and BsmBI-v2 kits (NEB) employing custom fusion sites ...
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bioRxiv - Synthetic Biology 2024Quote: ... or Gibson assembly (New England Biolabs). Plasmids were propagated in DH5alpha E ...
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bioRxiv - Neuroscience 2024Quote: ... 30 µl of protein A or G paramagnetic beads (73778S or 9006S; New England Biolabs) were washed and then complexed with primary antibodies or naïve rabbit IgG at 4°C with end-over-end rotation ...
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bioRxiv - Systems Biology 2024Quote: ... 5µl of RNA (roughly corresponding to 1µg) was reverse transcribed to cDNA using the LunaScript RT SuperMix kit - dye based qPCR detection (NEB) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purification of PCR products for downstream applications was accomplished using either phenol/chloroform ethanol precipitation or the Monarch® PCR & DNA Cleanup Kit (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: ... The HDRT integration site was PCR-amplified from genomic DNA using Q5 High-Fidelity Polymerase (NEB), with an expected amplicon size of ∼1kb for non-edited B2M alleles and ∼2kb for alleles with HDRT integration ...
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bioRxiv - Synthetic Biology 2024Quote: ... nucleic acids were digested by addition of 10 µL of DNaseI (2000 units/mL, New England Biolabs) and CaCl2 to a final concentration of 10 mM ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli (New England Biolabs, C3020K) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCRs were carried out using Q5 High-Fidelity DNA Polymerase (New England Biolabs). Following DpnI digest and PCR purification ...
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bioRxiv - Synthetic Biology 2024Quote: ... IVT-produced RNA samples were purified using a Monarch® RNA Cleanup Kit (New England Biolabs). ssRNA during RNA extractions was degraded with RNase T1 (Thermo Scientific) ...