Labshake search
Citations for New England Biolabs :
3251 - 3300 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Reprogramming enriches for somatic cell clones with small scale mutations in cancer-associated genesbioRxiv - Genomics 2020Quote: ... PCR products of 1400-1600bp were generated by running 20 PCR cycles with a Q5 Hot Start High Fidelity DNA Polymerase (NEB GmbH ...
-
bioRxiv - Genomics 2020Quote: ... PCR reactions were performed in 50 μl volumes with 1 ng pFA-MNase plasmid and the Q5 high fidelity polymerase (New England Biolabs). PCR thermocycling was executed as following ...
-
bioRxiv - Molecular Biology 2019Quote: ... were used in combination with mutation primers (Table 1) to generate overlapped PCR fragments (Phusion High-Fidelity DNA Polymerase, NEB), with disrupted RNA structure at each selected PAR-CL sites ...
-
bioRxiv - Cell Biology 2019Quote: ... the eGFP-FLAG-HA-MLKS2 sequence was PCR amplified with att flanking primers (Table S2) using high fidelity Q5 polymerase (NEB) and cloned into pDONR221 vector by BP cloning (Cat ...
-
bioRxiv - Immunology 2020Quote: ... The whole resulting product was then PCR-amplified using indexed primers with NEBNext High-Fidelity 2X PCR Master Mix (NEB). First ...
-
bioRxiv - Developmental Biology 2019Quote: ... Harvard Institute of Proteomics)(Zuo et al., 2007) using NheI and XbaI overhang primers and Phusion High-Fidelity Polymerase (NEB). A t2a-GFP backbone was subcloned from pLV hU6-sgRNA hUbC-dCas9-KRAB-t2a-GFP (Addgene plasmid #71237 ...
-
bioRxiv - Molecular Biology 2019Quote: ... The gene fragment covering the predicted middle domain (residues 807–1097) was amplified by PCR using Phusion High-Fidelity DNA Polymerase (New England Biolabs), using pcDNA3-Xpress-G5 as template (Francisco-Velilla et al. ...
-
bioRxiv - Cancer Biology 2020Quote: ... Custom oligos flanking the targeted sites were used to amplify genomic DNA from pooled edited cells (Supplementary Table 7) using High-Fidelity 2× Master Mix (New England Biolabs). Indel frequencies were quantified by comparing unedited control and knockout cell lines using Inference of CRISPR Edits (ICE)73.
-
bioRxiv - Developmental Biology 2019Quote: ... A short stretch of genomic region (∼600-800 bp) flanking the target site was amplified using Q5 Hot Start High Fidelity 2 × Master Mix (NEB). Amplified sequences were purified with the QIAquick PCR purification kit (Qiagen ...
-
bioRxiv - Genetics 2019Quote: ... Confirmation of the sequence of the alleles was achieved with the homozygous F2 fish using Phusion High-Fidelity DNA Polymerase (NEB) and Sanger sequencing.
-
bioRxiv - Microbiology 2019Quote: ... 1 kb fragments of the 5’ and 3’ flanks of the gene of interest (goi) ORF were generated by PCR using Phusion High Fidelity DNA polymerase (New England Biolabs) or Q5 High-Fidelity DNA polymerase (New England Biolabs ...
-
bioRxiv - Synthetic Biology 2019Quote: ... and 1 µL was used as the template in a PCR amplification containing 0.5 µL of Q5 High-Fidelity DNA Polymerase (NEB, M0491S), 1x Q5 polymerase reaction buffer (NEB ...
-
bioRxiv - Synthetic Biology 2019Quote: ... A PCR reaction adding a T7 promoter/terminator pair on each aptamer (see Table 1) was carried out using Q5 High-Fidelity DNA Polymerase (New England Biolabs Inc.(NEB) #M0491 ...
-
bioRxiv - Genomics 2019Quote: ... Libraries were produced by PCR amplification (12-14 cycles) of tagmented DNA using the NEB Next High-Fidelity 2x PCR Master Mix (New England Biolabs). Library quality was assessed in an Agilent Bioanalyzer 2100 ...
-
bioRxiv - Microbiology 2020Quote: ... in a new PCR tube 2.5 µL cDNA was combined with 12.5 µL Q5 High-Fidelity 2X Master Mix (NEB, Ipswich, USA), 8.8 µL nuclease free water (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... was used to generate thirteen amplicons covering the entire genome with the Q5 High-Fidelity DNA polymerase (New England Biolabs). PCR mixes (final volume 25µL ...
-
bioRxiv - Microbiology 2019Quote: The PCR reaction was motivated in 30 μL reaction systems after mixing 15μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers labelled with specific barcodes ...
-
bioRxiv - Plant Biology 2019Quote: HY5 genomic sequence was amplified from Col-0 gDNA using using Q5® High-Fidelity DNA Polymerase (New England Biolabs) and the primers HY5proFor-Not and HY5Rev-Sal ...
-
bioRxiv - Plant Biology 2019Quote: The promoter region and full-length CKX2I or coding DNA sequence (CDS) were amplified by PCR (Table S2) from genomic DNA or cDNA using Q5 High-Fidelity DNA Polymerase (NEB) and cloned either alone or under of the 35S promoter together with GFP and mScarlet-i into pPLV03 or pGEX5x3 using Gibson Assembly (NEB) ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... A region of the genome spanning all major reading frames and portions of the 5’ and 3’ UTRs was amplified (see Fig. S1 for primer sequences and locations) and Q5 High Fidelity DNA Polymerase (New England BioLabs) under the recommended conditions and 25 rounds of amplification ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... of HIV env was amplified using a nested PCR approach with Phusion High-Fidelity PCR Master Mix (New England Biolabs). The outer primers were ...
-
bioRxiv - Molecular Biology 2019Quote: ... Final libraries for each ChIP were produced using 150-200 ng of purified cDNA in a PCR reaction (High-Fidelity 2x master mix, New England BioLabs) for 8 cycles with 0.2 μM primers that carried a second 8bp barcode sequence ...
-
bioRxiv - Microbiology 2019Quote: ... 500 bp upstream and 500 bp downstream of the gene to be deleted were amplified by overlapping PCR with Q5 high fidelity DNA polymerase (NEB) using primers listed in Supplementary Table 1 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... the editing domain-coding segment of leuS gene was PCR amplified by using Phusion® High-Fidelity DNA Polymerase (NEB), primers 1 and 2 ...
-
bioRxiv - Genetics 2019Quote: The TDP-43 library was then prepared for deep sequencing by PCR amplification in two steps using Q5 High-Fidelity DNA Polymerase (NEB). In step 1 ...
-
bioRxiv - Microbiology 2019Quote: ... The full-length gene (with terminal NdeI and HindIII cut sites) was synthesized by overlap-extension PCR using Q5 polymerase with high GC buffer (NEB) and primers 979 and 987 (Supplemental Table 2) ...
-
bioRxiv - Cell Biology 2019Quote: ... All the OptoSrc and mutants plasmid construction were cloned in a Nhe1-Not1 digested pSico backbone amplified by PHUSION high fidelity DNA polymerase (NEB) using Gibson assembly (NEB ...
-
bioRxiv - Genomics 2019Quote: ... Ligated RNA was enriched with biotin-labeled products by another round of Streptavidin bead binding and washing (two washes each of High, Binding and Low salt buffers and one wash of 1x Thermo Pol Buffer (NEB)) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The cDNA was then used as template to amplify the envelope gene using High Fidelity Phusion DNA Polymerase (New England Biolabs). Nested PCRs were performed ...
-
bioRxiv - Biochemistry 2019Quote: ... fused to the 3’ end of the nucleotide sequence encoding full length Ypt7 was amplified by PCR from pET-19 Ypt7-tm (a kind gift from C Ungermann) with the Phusion high-fidelity DNA polymerase (NEB). The DNA fragment was cloned into BamHI and SalI digested pMBP-parallel1 vector (Sheffield et al. ...
-
bioRxiv - Cell Biology 2019Quote: ... Genomic regions of about 1 kb were amplified from wild-type genomic lysates using Q5 Hot Start high-fidelity polymerase (New England Biolabs) with the following primers ...
-
bioRxiv - Synthetic Biology 2019Quote: ... All the PCR reactions were performed according to the Phusion® High-Fidelity DNA Polymerase (New England Biolabs, MA, USA) protocol using 0.05 Units of Phusion and 100 µM dNTPs in a total volume of 25 µl.
-
bioRxiv - Molecular Biology 2019Quote: A 1 kb long DNA fragment with biotin molecules attached to the 5′ termini (dibiotin-DNA) was generated by PCR amplification using Q5 High-Fidelity Polymerase (NEB), with pAM075 as a template and the 5′-biotinylated primer pair oAM091/oAM092 ...
-
bioRxiv - Biochemistry 2020Quote: ... The region harboring the randomized hexamer and flanking constant tags was amplified from 1 µg ssDNA for 5 cycles using the Q5 Hot Start High-Fidelity PCR Master Mix (New England Biolabs) and the following primers ...
-
bioRxiv - Molecular Biology 2021Quote: ... PCR amplification was performed in 1X Phusion® High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs M0531) and PCR cycling at 98°C for 30s ...
-
bioRxiv - Genetics 2021Quote: ... DNA samples were digested with the rare-cutting enzyme PstI-HF (High-Fidelity; New England Biolabs Inc., Ipswich, MA, USA) and the common-cutting enzyme MspI (New England Biolabs Inc. ...
-
bioRxiv - Genetics 2021Quote: ... 10 ug of gel-purified mononucleosome DNA was prepared for high-throughput sequencing using the NEBNext DNA Library Prep Master Mix Set for Illumina (NEB). Sequencing was performed by the University of Oregon Genomics and Cell Characterization Core Facility.
-
bioRxiv - Genomics 2021Quote: ... Second PCR amplification with 8 cycles was used for generating sequencing-ready libraries using NEBNext High Fidelity master mix and Illumina Universal and Index primers (E7335S, New England Biolabs) in four and twelve parallel reactions on M-280 beads for day 2 and day 8 samples ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and re-amplified the size-selected libraries using a NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs, Canada). Finally ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Riboprobe templates were synthesized from cDNA via standard PCR using opsin specific primers (Table S1) and Q5 High Fidelity DNA polymerase (New England Biolabs). Primers were designed to bind to the coding sequence of target opsins (SWS2B ...
-
bioRxiv - Evolutionary Biology 2021Quote: We amplified pMini with different primer pairs for each mutation to be engineered (Table S5) using high fidelity Q5 polymerase (NEB). We transformed the PCR products into E.coli BW27784 cells made transformation-competent with the CaCl2 method60 ...
-
bioRxiv - Cell Biology 2021Quote: ... and DNA fragments were amplified by PCR with NEXTFlex primers (Primer 1 - 5’-AATGATACGGCGACCACCGAGATCTACAC; Primer 2 - 5’-CAAGCAGAAGACGGCATACGAGAT) and Phusion High-Fidelity DNA Polymerase (NEB) and further purified with AMPure XP beads to eliminate unligated primers and adapters ...
-
bioRxiv - Cell Biology 2021Quote: ... Libraries were amplified by PCR using NextFlex PCR primers (Primer 1 - 5’-AATGATACGGCGACCACCGAGATCTACAC; Primer 2 - 5’-CAAGCAGAAGACGGCATACGAGAT) and Phusion High-Fidelity DNA Polymerase (NEB) before three further rounds of AMPure XP purification were performed to collect fragments 150-300 bp in size ...
-
bioRxiv - Microbiology 2021Quote: PCR reactions to amplify the trailer sequences from the circularized cDNA using Q5 High-Fidelity DNA Polymerase (New England Biolabs) using 60 ng of circularized template were then set up with the following parameters ...
-
bioRxiv - Microbiology 2021Quote: ... Forward and reverse complementary primers consisting of the nucleotide codon sequence encoding for the mutation of interest were used to separately amplify the pMQ30 (for chromosomal mutations) or pMQ72 (ectopic expression) parental plasmids with the gene of interest using high fidelity Phusion polymerase (NEB). After four cycles of amplification ...
-
bioRxiv - Cancer Biology 2020Quote: ... PCR was carried out with primers targeting UPF1 exons 9 and 12 using Q5® High-Fidelity DNA Polymerase (NEB) (Table S5) ...
-
bioRxiv - Molecular Biology 2021Quote: ... genomic DNA isolated from Xanthomonas albilineans CFBP7063 was PCR amplified using Q5 Hot Start High-Fidelity 2X Master Mix (NEB) and cloned into pET28a using Gibson Assembly ...
-
bioRxiv - Microbiology 2020Quote: ... ∼1.2 kb upstream and downstream of the tcdR gene were PCR amplified with Phusion High-Fidelity DNA polymerase (NEB M0530S). All primers used for construction of the mutagenesis vector and for PCR screening of transconjugants can be found in Supplemental Table 1 ...
-
bioRxiv - Microbiology 2021Quote: ... All primers (Supplementary Table 2) were purchased from Eurofins Genomics and all PCR reactions were performed using Q5 High-Fidelity (New England Biolabs). HIV-1NL4-3 (pHIV-1WT ...
-
bioRxiv - Biophysics 2021Quote: ... Hybridization reactions were split into two and libraries re-amplified using Post LM-PCR oligos (Nimblegen) and Q5 High-Fidelity DNA polymerase (NEB) directly from the beads ...