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Citations for New England Biolabs :
3051 - 3100 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: All DNA fragments generated by PCR for cloning were amplified using the Q5 High-Fidelity DNA polymerase (NEB, USA), as indicated by manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... A 470 bp PCR product surrounding the UL23 cut site was amplified using Phusion high-fidelity polymerase (NEB, USA) and column purified (Macherey-Nagel ...
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bioRxiv - Microbiology 2020Quote: ... All PCR reactions were performed using Q5© high-fidelity DNA polymerase [2,000 units/ml] (New England Biolabs, Germany) in triplicates ...
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bioRxiv - Microbiology 2020Quote: ... Phage genes were amplified from Butters genomic DNA by PCR using Q5 High-Fidelity DNA polymerase (New England Biolabs). All PCR products contained the entire 179bp between gene 29 and gene 30 (containing the endogenous promoter and ribosome binding site [RBS] ...
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bioRxiv - Cell Biology 2021Quote: ... NCBI accession number NM_001098802) was used as template for amplification with the Phusion High-Fidelity DNA polymerase (New England BioLabs) under standard PCR conditions and attB forward and reverse primers (see Key Resources Table ...
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bioRxiv - Cell Biology 2021Quote: ... The targeting genomic PCR in CCR5-mutated clones was performed using Q5 Hot Start High Fidelity DNA polymerase (NEB) with the following primers ...
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bioRxiv - Bioengineering 2022Quote: ... The DNA fragments were amplified by PCR using Q5® High-Fidelity DNA polymerase (New England Biolabs Inc., USA).
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bioRxiv - Microbiology 2022Quote: ... two multiplex PCR tiling reactions were prepared by combining 2.5 μL cDNA with 12.5 μL Q5 High-Fidelity 2X Master Mix (NEB, USA), 6μL nuclease free water ...
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bioRxiv - Microbiology 2022Quote: ... and a 956 bp fragment of omp-pst2 gene was amplified using the primers OmpPst2_F4qs (5’-ATTATTCGCGGCGGGTGTTAC-3’) and OmpPst2_R4qs (5’-CAGCGGCCATATTCTTGTTGA-3’) using the Q5 High-Fidelity DNA Polymerase (New England BioLabs). The PCR program consisted in an initial step at 98°C for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... according to the manufacturer’s instructions and was then amplified using 0.02 U/ μl of Q5® High-Fidelity DNA Polymerase (NEB) according to manufacturer’s instructions with the primer sets listed in Supplementary Table 1.
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bioRxiv - Immunology 2022Quote: ... and three fragments covering the full-length genome were synthesized with high-fidelity Phusion HiFi PCR Master Mix (NEB,) using specific primers as shown by Yang et al ...
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bioRxiv - Genetics 2022Quote: ... All PCR reactions were conducted using Q5 High-Fidelity 2X PCR Master Mix following the manufacturer’s directions (New England Biolabs). These fragments were transformed into S ...
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bioRxiv - Developmental Biology 2020Quote: ... standard PCR reactions were performed on 1 ul of cDNA using Q5 High-Fidelity DNA Polymerase (New England Biolabs) and each of the variant PCR primer pairs independently ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplifications were carried out using high fidelity Phusion polymerase or Taq DNA polymerase (both from New England Biolabs). Electroporation of C ...
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bioRxiv - Molecular Biology 2020Quote: ... All PCRs for library preparation were carried out using Q5 High-Fidelity 2X Master Mix (NEB, Cat. No. M0492L). An initial enrichment amplification of 15 cycles was followed with a second round of PCR using unique P5 and P7 indexing primer combinations for 15 cycles and purified using 1.8X SPRI beads ...
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bioRxiv - Microbiology 2021Quote: ... cDNA across the samples was normalised and specific transcripts amplified using Phusion® high-fidelity PCR master mix (NEB) with primers outlined in Supplemental Table S2 ...
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bioRxiv - Microbiology 2021Quote: ... First-round PCR of 14 cycles was performed using Q5 High-Fidelity Master Mix (New England BioLabs, Ipswich, MA) and nested gene-specific primers at 20 μM ...
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bioRxiv - Neuroscience 2020Quote: ... primers were designed using NEBuilder Assembly Tool (v1.12.18) to generate overlapping (20-25 bps) PCR fragments amplified using Q5® High-Fidelity DNA Polymerase (NEB). Modified TALE constructs were created using PCR fragments amplified from pAAV-CW3SL-EGFP (Addgene # 61463) ...
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bioRxiv - Cancer Biology 2021Quote: PCR amplifications were carried out using Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB, Cat#M0531L) according to the manufacturer’s instructions (25 µl reaction) ...
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bioRxiv - Synthetic Biology 2021Quote: All PCR reactions for cloning purposes were performed using Q5® High Fidelity 2X Master Mix (New England Biolabs). The reactions mixtures were prepared using 1 ng of template ...
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bioRxiv - Genetics 2022Quote: ... PCR reactions were performed using NEBNext® High-Fidelity 2X PCR Master Mix (New England BioLabs, catalog number M0541) following the manufacture’s protocol unless specified differently ...
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bioRxiv - Developmental Biology 2022Quote: pTol2 HS:cachd1,GFP: Full-length cachd1 was amplified from cDNA using high-fidelity Phusion DNA polymerase (New England Biolabs) and then phosphorylated with PNK (New England BioLabs ...
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bioRxiv - Microbiology 2019Quote: ... Chromosomal deletion strains were constructed using the allele exchange vector pKAS32 digested with KpnI and SacI (NEB, High Fidelity). Luciferase reporters were constructed using the luciferase reporter vector pBBRlux digested with BamHI and SpeI (NEB) ...
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bioRxiv - Microbiology 2019Quote: ... The reverse transcription was followed by PCR amplifications using the Q5® high-fidelity DNA polymerase (New England Biolabs) with primers amplifying two overlapping fragments per RNA segment ...
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bioRxiv - Genomics 2019Quote: ... We added 1 μL 12.5% Triton-X to each well to quench the SDS and 12.5 μL NEBNext High-Fidelity 2× PCR Master Mix (NEB). Samples were PCR-amplified for 12 cycles (72 °C 5 min ...
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bioRxiv - Neuroscience 2019Quote: Enhancers were cloned from C57Bl/6J genomic DNA using enhancer-specific primers and Phusion high-fidelity polymerase (M0530S; NEB). Individual enhancers were then inserted into an rAAV or scAAV backbone that contained a minimal beta-globin promoter ...
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bioRxiv - Immunology 2019Quote: ... First-round Ig-encoding sequence amplification (20 cycles) was performed using Q5 High-Fidelity Master Mix (New England BioLabs) and nested gene-specific primers ...
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bioRxiv - Microbiology 2019Quote: ... Ten ng DNA isolated from vegetative cells grown in AA/8 and AA/8N and 10 ng DNA isolated from heterocysts were added to a 20-μL reaction containing 0.2 units of Phusion High-Fidelity DNA Polymerase (NEB), 1X Phusion buffer ...
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bioRxiv - Microbiology 2020Quote: ... Template DNA was amplified in duplicate 20 μl reactions containing 1.0 U Q5 high fidelity DNA polymerase (NEB, UK), 0.25 μM primers ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 50 ul PCR reactions were set up with approximately 1 ug cDNA and Phusion High-Fidelity DNA Polymerase (NEB) was used ...
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bioRxiv - Immunology 2020Quote: ... The ligated plasmids pAF217 and pAF218 were then transformed into NEB® Stable Competent Escherichia coli (High Efficiency) (NEB). Single colonies were selected ...
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bioRxiv - Microbiology 2020Quote: ... Amplification was performed on 4 ng of pKD3 plasmid using Q5 High-Fidelity 2X Master Mix (New England Biolabs). The PCR product was digested for 1 hour with the restriction enzymes DpnI and ClaI at 37°C and then the PCR product was run on a 1% agarose gel ...
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bioRxiv - Genomics 2021Quote: ... AK4P3 and RPL13AP20 were amplified from cDNA with Q5® High-Fidelity DNA Polymerase (New England Biolabs cat # M0492S) using primers that amplify the novel full-length transcript and contain HindIII and NotI restriction sites (Table S5) ...
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bioRxiv - Synthetic Biology 2021Quote: Linear DNA templates for expression in IVTT reactions were created by PCR using Phusion High-Fidelity DNA Polymerase (NEB) with primers pBEST_LinL_F and pBEST_LinL_R (Table S1 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We engineered the Thrβ119Ser substitution by whole plasmid amplification using mutagenic primers and Phusion High-Fidelity DNA Polymerase (New England BioLabs), phosphorylation with T4 Polynucleotide Kinase (New England BioLabs) ...
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bioRxiv - Genomics 2019Quote: ... We added 1 µL 12.5% Triton-X to each well to quench the SDS and 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB). Samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Genomics 2019Quote: ... we enriched the purified DNA templates through PCR using Phusion Hot Start High-Fidelity DNA Polymerase (NEB, M0530S, USA). After purification of the PCR products (MinElute PCR Purification Kit ...
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bioRxiv - Genomics 2019Quote: ... Template DNA (genomic DNA from HeLa S3 cells) was amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs) to generate NCAPH homology arms ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were amplified using a one-step barcoding PCR using NEBNext High Fidelity 2X PCR Master Mix (NEB, M0541L) and the following primers:
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bioRxiv - Molecular Biology 2019Quote: ... then amplified and indexed with unique library indices using NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs). PCR products were purified with AMPure XP beads (Beckman-Coulter ...
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bioRxiv - Genetics 2021Quote: ... We added 1 µL 12.5% Triton-X to each well to quench the SDS and 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB). Samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Genetics 2020Quote: ... PCR analysis was carried out with different primer sets (Supplementary Table 1, Fig. 2B) using Phusion High-Fidelity DNA Polymerase following the manufacturer’s instructions (New England Biolabs).
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bioRxiv - Molecular Biology 2020Quote: σA (MSMEG_2758) and rbpA (MSMEG_3858) genes were amplified from genomic DNA by PCR with Phusion High-Fidelity DNA Polymerase (NEB) with primers #1155 + #1156 (σA ...
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bioRxiv - Synthetic Biology 2021Quote: Sub-parts (modules, Supplemental Table 1) were amplified via high-fidelity polymerase chain reaction (PCR) (New England Biolabs #M0491S) using dsDNA templates and ssDNA primers listed in Supplemental Table 2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The purified DNA was amplified as described before with NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs). The PCR amplified product was cleaned twice with (0.9X ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated by 7 rounds of PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L), purified using SPRIselect reagent kit (Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: ... of SEMA3C (ENST00000265361.7) were PCR amplified from neonatal LEC cDNA using Phusion high-fidelity DNA polymerase (New England BioLabs), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: All cloning PCR amplifications were performed using the Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich MA) according to standard procedures.
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bioRxiv - Microbiology 2020Quote: ... Amplification of regions of interest was achieved through PCR using Q5 high-fidelity DNA polymerase (New England Biolabs Inc.). The total reaction volume of 50μL consisted of the 200ng/μL DNA extract ...
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bioRxiv - Microbiology 2020Quote: ... 2μL of the resulting mix was transformed into chemically competent Escherichia coli (High efficiency DH5-alpha, New England Biolabs) according to manufacturer’s instructions and cultured for 16 hours on Luria broth (LB ...