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Citations for New England Biolabs :
3101 - 3150 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Point mutations were introduced with a QuikChange site-directed mutagenesis protocol using Q5 High-Fidelity 2x Master Mix (NEB). NLRP3 mutants were named ...
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bioRxiv - Immunology 2020Quote: ... Ighγ1 heavy-chain sequences were amplified in a nested PCR using primers for the Ighγ1 constant region together with a specific primer for VH186.2 (Mayer et al., 2017)(Table S1) and using high-fidelity Q5 polymerase (NEB). Amplified products were cloned (CloneJET PCR Cloning Kit ...
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bioRxiv - Cell Biology 2020Quote: ... A two-step PCR was used to assemble pUR19-adh1-Cas9-rrk1-sgRNA:Fex1+ with a guide sequence targeting bgs1 using Q5 High-Fidelity polymerase (New England BioLabs). pUR19-adh1-Cas9-rrk1-sgRNA:Fex1+ and a 400-bp repair guide fragment containing the bgs1D277N mutation were introduced into fex1Δ fex2Δ S ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNAs encoding human elongation factor eEF2 were amplified by PCR using Q5 High-Fidelity 2X Master Mix (NEB) with primers (forward primer ...
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bioRxiv - Genomics 2020Quote: ... PCR products with sticky ends were highly diluted to a final concentration less than 1ng/μl and ligated with a high concentration (66.68U/uL) of T4 DNA ligase (New England BioLabs) at 16°C for at least 16 hours ...
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bioRxiv - Genomics 2021Quote: ... 750 ng of DNA was digested overnight with 20 units of high fidelity SspI restriction enzyme (New England Biolabs) in Cutsmart® buffer and electrophoresed through a 1% agarose (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNAs encoding the Senp5 open reading frame (ORF) were amplified by Q5 High-Fidelity DNA Polymerase (NEB Japan) with primer sets based on the predicted sequences of the mouse Senp5 isoforms (GenBank accession numbers ...
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bioRxiv - Molecular Biology 2020Quote: ... All fragments used for the cloning step were amplified via PCR using Q5 high fidelity DNA polymerase (NEB #M0491S) (table S4 ...
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bioRxiv - Cancer Biology 2020Quote: ... The cDNA was used as a template for amplification by Phusion high-fidelity DNA polymerase (New England Biolabs, M0530L) in PCR and PIWIL1 was cloned into the pcDNA3.1-3xFlag.
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bioRxiv - Microbiology 2020Quote: ... PCR reactions for cloning were performed by using Q5® High-Fidelity DNA Polymerase (New England Biolabs, Inc (NEB). All the products sequenced to verify the absence of any mutations (GATC-biotech).
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bioRxiv - Microbiology 2022Quote: ... The first fragment was generated by amplifying the GFP from pcDNA3.1-GFP (a gift from Carolyn Coyne, Duke University) using Q5 high-fidelity DNA polymerase (New England Biolabs) with NheI-GFP_F (5’-GAAGCTAGCCACCATGGTGAGCAAGGGCGAGGAG-3’ ...
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bioRxiv - Biophysics 2022Quote: ... and inserted to desired positions into the pCDNA.3.1-mPIEZO1 plasmid using High-Fidelity DNA Assembly (New England Biolabs). The presence of cpGFP inserts was confirmed by Sanger sequencing (GENEWIZ) ...
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bioRxiv - Molecular Biology 2022Quote: ... The RT reaction was then amplified using PHUSION® high-fidelity DNA polymerase using HF buffer (New England Biolabs) via PCR to generate a 1675-bp amplicon ...
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bioRxiv - Molecular Biology 2022Quote: ... The new construct was created from a PCR reaction that amplified the entire plasmid harbouring the substitution using the high-fidelity polymerase Q5 according to the manufacturer’s instructions (New England Biolabs; NEB). The unpurified PCR product was then treated with DpnI to digest the methylated template DNA which was subsequently transformed into DH5α ...
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bioRxiv - Genomics 2022Quote: ... We then amplified the barcodes from each clone using Q5 High Fidelity 2X Master Mix (M0492, New England Biolabs) with primers specific to our reporter gene (CAS P58-59) ...
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bioRxiv - Genomics 2022Quote: ... Each PCR was run using 12.5μL Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA), 2.5μL template DNA ...
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bioRxiv - Genetics 2022Quote: ... 1 µL of extracted genomic DNAs and 5 µL of 2× Hot Start High Fidelity Q5 Master Mix (NEB). PCR reactions were carried out under the following condition ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA standards (ssDNA standards) were amplified from pRnc-gRNA DNA using Phusion High Fidelity DNA Polymerase (New England BioLabs) with the pWP252fluor reverse primer and forward primers annealing to the expected 5’ termini of the cleaved and uncleaved 5’UTR that would yield the desired size products ...
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bioRxiv - Molecular Biology 2022Quote: ... the products were amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs) with 200 nM of Illumina multiplex primer and 200 nM of Illumina index barcode primers (98°C for 10 sec pre-denaturation followed by 12 cycles of 98°C for 5 sec ...
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bioRxiv - Molecular Biology 2023Quote: ... The sir2 ORF was amplified by PCR using Q5 High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA, USA) and cloned into p123 within the NcoI and NotI restriction sites of p123 ...
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bioRxiv - Microbiology 2023Quote: ... Phage genes were amplified from Butters genomic DNA by PCR using Q5 high-fidelity DNA polymerase (New England Biolabs). PCR products were digested with XbaI overnight (O/N) ...
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bioRxiv - Zoology 2023Quote: ... We performed 5 PCR enrichment with 15 cycles (30 ng DNA input, NEB Phusion High-Fidelity PCR Master Mix) for each library to increase fragment diversity ...
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bioRxiv - Plant Biology 2023Quote: ... The GdMYBSG6 coding sequences were amplified from Spring morphotype cDNA using Phusion High-Fidelity DNA Polymerase (New England Biolabs) and cloned into pBLUESCRIPT KS(- ...
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bioRxiv - Microbiology 2024Quote: Test PCRs on cDNA were performed in a 50 µL scale using 1.0 µL precipitated cDNA in the presence of 1 x High-Fidelity PCR MasterMix (NEB), 2 µM forward primer with native barcode and 2 µM reverse primer with native barcode ...
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bioRxiv - Plant Biology 2022Quote: ... HvMON1i and CSEP0162 were amplified from Bgh- inoculated barley (Ingrid) cDNA by Q5® High-Fidelity DNA Polymerase (NEB). The primers are listed in Supplementary Table S1 ...
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bioRxiv - Microbiology 2022Quote: ... Conventional PCR assays were carried out with Q5 Hot Start High-Fidelity 2× Master Mix (New England Biolabs, Australia). For some samples ...
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bioRxiv - Cancer Biology 2024Quote: ... The purified DNA was amplified as described before with NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs). The PCR amplified product was purified and size selected twice with AMPure beads (Beckman).
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bioRxiv - Biochemistry 2024Quote: ... The PCR reaction was mixed by combining 25 μL of Q5 High-Fidelity DNA Polymerase (New England Biolabs #M0494S), 2 μL of the oligo pool solution ...
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bioRxiv - Biochemistry 2024Quote: ... All truncated or tagged versions of clones were generated using Q5 high-fidelity DNA polymerase (New England Biolabs #M0515) and NEB-Gibson Assembly (New England Biolabs #E2611S) ...
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bioRxiv - Cancer Biology 2024Quote: ... the pooled oligo library was amplified using NEB Next High-Fidelity PCR Master Mix (New England Biolabs, cat#M0541S). The PCR product was digested with Esp3I (BsmBI ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed with medium range primers 24 using Q5 High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The second strand synthesis was then performed by adding 100 μl Q5 Hot Start High Fidelity Master Mix (NEB), 10 μl RNase H (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... Input genomic DNA was first amplified in a 10μL reaction for 30 cycles using NEBNext High-Fidelity 2×PCR Master Mix (NEB). Amplicons were purified using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR reactions were performed using Phusion® high-fidelity DNA polymerase according to the manufacturer’s protocol (New Englang BioLabs). Amplified flanking recombination regions were merged with the MX cassette through Circular Polymerase Exchange Cloning into the pUC19 plasmid [67] ...
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bioRxiv - Microbiology 2024Quote: Final PCRs on cDNAs were performed in a 100 µL scale using 2.0 µL precipitated cDNA in the presence of 1 x High-Fidelity PCR MasterMix (NEB), 2 µM forward primer with native barcode and 2 µM reverse primer with native barcode (e.g BC1 fwd and rev ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of isolated phage T4 in Pi-Mg buffer was used as a PCR template in the first PCR with the following reaction mix: 0.125 µM each primer (Supplementary Table 2) in 1x High Fidelity Master Mix (NEB) with a total volume of 10 µl ...
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bioRxiv - Microbiology 2023Quote: ... followed by a dephosphorylation step using the shrimp alkaline phosphatase (Phusion High-fidelity DNA polymerase, New England Biolabs, France). hcp and tssB flanking regions were PCR-amplified from CFBP13503 with the Phusion High-Fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... ATAC-seq libraries were prepared by ∼8 cycles of PCR using NEBNext High Fidelity 2X PCR Master Mix (NEB) and primers containing Illumina barcodes (Buenrostro et al ...
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bioRxiv - Genomics 2023Quote: ... Accessible DNA libraries were amplified by PCR using NEBNext High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA), custom Nextera PCR primers as previously described (29) ...
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bioRxiv - Genomics 2023Quote: ... The number of library amplification cycles was determined by qPCR analysis using NEBNext High-Fidelity PCR MasterMix (NEB, M0541) on LightCycler® 96 System (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... Addgene cat#52961) by PCR amplification using NEB Next High-Fidelity PCR Master Mix (New England Biolabs, cat#M0541S). The PCR product was digested with Esp3I (BsmBI ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fragments for Gibson assembly were generated by PCR using Q5 Hot Start High-Fidelity polymerase (New England Biolabs, USA). PCR products were analyzed by agarose gel electrophoresis and bands of the expected size were excised ...
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bioRxiv - Microbiology 2023Quote: ... PCR analysis of total DNA extracted from oysters (N=60) used the high fidelity Q5 polymerase (New England Biolabs) in a total volume of 50 μL under the following conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... Amplicons for long-range sequencing were generated with Q5 High-Fidelity polymerase in 100 uL reactions (New England Biolabs) including500 nM primers and up to 1000 ng genomic DNA using the following PCR protocol ...
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bioRxiv - Microbiology 2023Quote: ... The 30 μl PCR reaction system contained 15 μl of Phusion high-fidelity PCR Master Mix (New England Biolabs), 0.2 μM forward and reverse primers ...
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bioRxiv - Molecular Biology 2023Quote: ... Deletion variants of ATI2 were amplified from ATI2 (AT4G00355.1) CDS by overlap extension PCR using Phusion High Fidelity DNA polymerase (NEB) and subcloned into pCR™4/TOPO™ vectors (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... N- and C-terminal linkers of R-iLACCO0.1 were deleted using Q5 high-fidelity DNA polymerase (New England Biolabs) to provide variants with different linker length ...
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bioRxiv - Genetics 2023Quote: ... The PCR reaction was performed using NEBNext® High-Fidelity 2× PCR Master Mix (New England BioLabs, catalog #M0541) with a reaction volume of 10 ul including 5 ul 2× PCR Master Mix ...
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bioRxiv - Neuroscience 2023Quote: SadCas9-2xKRAB-p2a segment was amplified by PCR from backbone using high-fidelity polymerase (Phusion, NEB, Cat. No. M0530S); and tdTomato was PCR amplified from a proprietary expression vector using high-fidelity polymerase ...
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bioRxiv - Genetics 2023Quote: ... The target loci were PCR amplified in 30 PCR cycles using Q5 High-Fidelity DNA Polymerase (New England Biolabs), locus-specific primer pairs (Supplementary Information ...