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Citations for New England Biolabs :
3451 - 3500 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Amplification of products for next-generation sequencing was conducted with Q5 Hot Start High-Fidelity 2X Master Mix (New England BioLabs). All DNA oligonucleotides used in this study were obtained from Integrated DNA Technologies ...
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bioRxiv - Genetics 2020Quote: ... three selected guide RNAs based on their high Guide Design Tool scores and low off-target probabilities were assessed by the T7 endonuclease I (NEB) assay per manufacturer’s direction ...
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bioRxiv - Genomics 2020Quote: ... 10uL tagmented DNA prepared as described above was used in a 25uL PCR reaction using NEBNext High-Fidelity Master Mix (New England Biolabs) and Nextera XT Dual-Indexed primers (Nextera) ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplification of DNA fragments for cloning was carried out using the high-fidelity DNA polymerase Q5 (New England BioLabs: NEB). Taq polymerase (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... VARV C3L and CMLV 032 single and overlapping mutations were generated by site-directed mutagenesis using Phusion High-Fidelity DNA polymerase (NEB). All ORFs were validated by Sanger sequencing (UC Davis sequencing facility) ...
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bioRxiv - Microbiology 2020Quote: ... into cDNA which was used in two-round nested PCR for amplification of envelope gene using High Fidelity Phusion DNA Polymerase (New England Biolabs). The envelope amplicons were purified ...
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bioRxiv - Molecular Biology 2021Quote: ... from RNA-seq samples was used as input for PCR amplification with Phusion High-Fidelity DNA polymerase (New England Biolabs) and UPF1 specific primers that flank the regulatory loop sequence (Forward ...
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bioRxiv - Molecular Biology 2021Quote: ... The pre-gRNA cassette was PCR amplified using Q5® High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA, USA) to encode MluI and BamHI digestion sites as described in Supplementary Table S2 ...
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bioRxiv - Immunology 2021Quote: ... Library DNA was synthesized as a pooled oligo library by BioXp and amplified with High Fidelity Phusion polymerase (New England Biolabs). PCR products were gel extracted (Qiagen Gel Extraction kit ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were carried out as described above (25 μL total volume, Q5 hot start high fidelity 2X master mix [New England Biolabs] ...
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bioRxiv - Developmental Biology 2020Quote: ... 2 PCR2 reactions were used per timepoint with 5 μL of PCR1 product amplified with 25 μL Q5 High Fidelity 2X Master Mix (NEB), 5 μL of 10 μM primer mix in 50 μL volume using the following PCR protocol ...
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bioRxiv - Genomics 2021Quote: ... was amplified and barcoded in parallel using the same PCR program in 50 μL PCR reaction (2 μL gap-repaired DNA, 25 μL 2× NEBNext High-Fidelity PCR Master Mix (NEB), 1.5 μL 10 μM i5 universal PCR primer ...
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bioRxiv - Genomics 2021Quote: ... 95°C/15 s and annealing/extension: 65°C/5 min) of PCR using Q5 high-fidelity DNA polymerase (NEB). Amplicons were purified using 1× AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplification of DNA fragments for cloning was carried out using the high-fidelity DNA polymerase Q5 (New England BioLabs: NEB). Taq polymerase (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... Pre-amplified PCR products were transferred to 96-well plates and further amplified for an additional 13 cycles using custom Nextera dual-index primers and NEBNext High-Fidelity 2X PCR master mix (New England Biolabs). Individually barcoded libraries were pooled and purified on a single MinElute column (Qiagen) ...
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Treatment history shapes the evolution of complex carbapenem-resistant phenotypes in Klebsiella spp.bioRxiv - Microbiology 2022Quote: The coding sequences of β-lactamase and DeepBL candidate genes were amplified from FK688 genomic DNA using Fusion High-Fidelity DNA polymerase (New England BioLabs) with the oligonucleotide primers listed in Table S10 ...
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bioRxiv - Cancer Biology 2022Quote: ... A 200-275 base pair region containing the relevant sgRNA target sequence was PCR-amplified from genomic DNA using the NEBNext High-Fidelity 2X PCR Master Mix (New England BioLabs) in a 25 µL PCR reaction volume (primer sequences appear below) ...
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bioRxiv - Cancer Biology 2022Quote: ... The locus-specific HDR donors were generated by PCR amplification of the MACHETE bicistronic cassette using a high-fidelity DNA polymerase (Herculase II, Agilent or Q5, NEB). PCR fragments were column purified (Qiagen ...
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bioRxiv - Biochemistry 2022Quote: ... 3.75 μl (corresponding to ~7,500 cells) of lysate was used as a template for PCR amplification with Q5 Hot-Start High Fidelity DNA Polymerase (NEB) and unique primer pairs containing an internal locus-specific region and an outer Illumina-compatible adapter sequence ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplification was carried out using NEB Q5 High Fidelity 2x Master Mix (New England Biolabs Inc., Ipswich, Massachusetts, USA). The PCR reaction consisted of 2.5 ul each of 10 μM Forward and Reverse Primers ...
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bioRxiv - Molecular Biology 2022Quote: Promoter regions were PCR amplified from S2 cell genomic DNA using primers containing gibson overhangs corresponding to the BglII and HindII restriction sites on pGL3 with Q5 high-fidelity 2x master mix (NEB). PCR products were cleaned with AMPURE beads and eluted in water ...
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bioRxiv - Molecular Biology 2022Quote: ... The new construct was created from a PCR reaction that amplified the entire plasmid harbouring the substitution using the high-fidelity polymerase Q5 according to the manufacturer’s instructions (New England Biolabs; NEB). The unpurified PCR product was then treated with DpnI to digest the methylated template DNA which was subsequently transformed into DH5α ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were then subjected to library RT using random octamers (63) and to cDNA amplification thereafter using the Phusion High-Fidelity DNA Polymerase (New England Biolabs). A forward primer sequence against the 5’ Illumina adapter (63 ...
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bioRxiv - Bioengineering 2022Quote: All gene amplifications were performed using polymerase chain reaction (PCR) in a 50 µL mix with Q5 High-Fidelity DNA Polymerase (New England Biolabs) according to the manufactureŕs protocol ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: ... RT-PCR was performed on cDNAs from murine Th17 or human HEK293 cells using the Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs) with the standard thermocycling protocol ...
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bioRxiv - Biochemistry 2022Quote: ... PDGFRβ DNA inserts containing the desired mutations were generated by PCR amplification using the Phusion High-Fidelity DNA Polymerase (New England Biolabs) and subcloned into pLenti CMV Hygro DEST for cellular studies ...
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bioRxiv - Biochemistry 2022Quote: ... KIT DNA inserts containing the desired mutations were generated by PCR amplification using the Phusion High-Fidelity DNA Polymerase (New England Biolabs). The inserts were subcloned into either pFastBac 1 vector for structural studies or pBABE-puro vector for cell-based studies ...
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bioRxiv - Cancer Biology 2022Quote: PCR products were amplified from the specified genomic DNA samples with Q5 High-Fidelity 2X Master Mix (New England BioLabs) according to the manufacturer’s protocol ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... DNA fragments were amplified with 15-20 bp of homology regions in primers using Phusion R High Fidelity DNA polymerase (New England Biolabs; NEB) and homology based assemblies were performed using NEBuilder® HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... A 4699 bp HDR template dsDNA (HDRT) was generated by amplification from a plasmid template using the Q5 High-Fidelity 2X Master Mix (New England BioLabs) with primers containing truncated Cas9 target sequences (IDT ...
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bioRxiv - Biochemistry 2022Quote: The DNA templates for the different RNA designs were produced by PCR amplification using Phusion High-Fidelity DNA polymerase (NEB) of double stranded gene fragments (gBlocks ...
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bioRxiv - Bioengineering 2022Quote: Genomic DNA samples were amplified with PCR using Q5 Hot Start High-Fidelity 2X Master Mix (New England BioLabs M0494). Primer pairs for all sequences are listed in Table S3 ...
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bioRxiv - Biophysics 2022Quote: ... The mutations were introduced into the huVinculin coding sequence by the TPCR method62 using Q5 High-Fidelity Master Mix (NEB). In the initial step the 4 mutations (K944A_R945A_D1013A_E1015A ...
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bioRxiv - Developmental Biology 2022Quote: ... musculus was cloned into the pOPIN expression vector using the SLIC method and Phusion Flash High-Fidelity PCR Master Mix (Finnzymes/New England Biolabs). SLIC reactions were then transformed into One Shot™ OmniMAC™ 2 T1® Chemically Competent E ...
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bioRxiv - Cell Biology 2022Quote: ... the DNA template was amplified by PCR using Phusion High-Fidelity DNA polymerase and Phusion GC Buffer (New England Biolabs). The PCR product was purified using the DNA Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid hcm1 sequence was amplified by PCR for 21 cycles with vector specific primers using Phusion High-Fidelity DNA polymerase (New England Biolabs). Products were extracted from a 1% agarose gel using the QIAquick Gel Extraction Kit (Qiagen) ...
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bioRxiv - Genomics 2022Quote: ... Transposed DNA fragments were amplified for 13 cycles in the presence of Custom Nextera PCR primers (Buenrostro et al., 2013) using the NEBNext High-Fidelity 2x PCR Master Mix (Cat. #M0541, New England Biolabs). Libraries were purified using the High Pure PCR Production Purification Kit (11732676001 ...
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bioRxiv - Genetics 2022Quote: Genotyping samples of kcnh6a editants submitted to Sanger sequencing (Eurofins Genomics) were PCR amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs) and locus-specific primers (kcnh6a_fwd 5’-GCTTTGCAAGGTATAGAGCACAG-3’ and kcnh6a_rev 5’-AACGTTGCCAAAACCCACAC-3’ ...
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bioRxiv - Immunology 2022Quote: ... cells were brought to 5×105 cells/ml and incubated with transposition reaction mix (NEBNext High-Fidelity 2X PCR master mix, NEB) for 30 min ...
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bioRxiv - Genetics 2022Quote: ... To confirm library composition and even sgRNA representation by deep-sequencing a PCR reaction was carried out to add illumina adaptors and a barcode by using the Phusion High Fidelity DNA Polymerase (New England Biolabs), with an annealing temperature of 56 °C and 15 cycles (LR177/LR175 ...
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bioRxiv - Genetics 2022Quote: ... 5 ng of the synthesized oligo pool were carried out using the Phusion High Fidelity DNA Polymerase (New England Biolabs), with a total of 15 cycles and an annealing temperature of 56 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We amplified the wild-type EST1 gene from the genome of the haploid strain BY4742 by polymerase chain reaction (PCR) using the high-fidelity Q5 polymerase (NEB) and inserted it into the ΔEST1 cells used in [1] by CRISPR/Cas9 editing ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All single-codon substitutions were generated by inverse PCR with Phusion High Fidelity polymerase in HF Buffer (New England Biolabs) using degenerative codon NNN targeted to each codon of the rnc gene ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 40bp downstream) were PCR-amplified from the PN16 (IncI2) plasmid using Q5® High-Fidelity DNA Polymerase (New England BioLabs). The amplified fragment was cloned into pSEVA121 using the NEBuilder® HiFi DNA Assembly kit (New England BioLabs ...
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bioRxiv - Plant Biology 2022Quote: ... Individual clones were selected and confirmed via colony PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA) with primers 1BF and 8R which were designed to amplify the coiled coil and NB-ARC domain coding sequences of MRI-R1 ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by a single-cycle of PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs) (98°C for 10 sec pre-denaturation followed by 98°C for 5 sec ...
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bioRxiv - Molecular Biology 2022Quote: ... The thyA gene region was amplified from the genomic DNA (5 ng) with Phusion High-Fidelity PCR Master Mix (New England Biolabs) using 200 nM of thyA forward and reverse primers that give amplicons of 750 bp ...
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bioRxiv - Molecular Biology 2022Quote: ... Illumina multiplex and bar code primers were added by PCR (1 µL of MMEJ product and 200 nM primers in Phusion High-Fidelity PCR Master Mix (New England Biolabs) with 98°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR was done with 1-2 ng of plasmid and 200 nM of each primer in Phusion High-Fidelity PCR Master Mix (New England Biolabs) with pre-denaturation at 98°C for 5 sec followed by 12 cycles of 98°C for 5 sec ...
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bioRxiv - Neuroscience 2022Quote: ... The heavy-chain variable domain was then amplified from the cDNA using Q5 high-fidelity DNA polymerase (New England Biolabs) with the described primers (CALL001 ...