Labshake search
Citations for New England Biolabs :
3051 - 3100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... crosslinked RNA fragments were dephosphorylated at their 3’-ends using T4-PNK (NEB, M0201S) and ligated to a pre-adenylated 3’-adapter (L3-App ...
-
bioRxiv - Biochemistry 2024Quote: The cloning of human wild type Mad2 and the mutant Mad2R133A by the USER® method (NEB) into the pRSFDuet-1 vector (71341-3 ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 µl T4-PNK (10 U/µl, NEB) and 9.5 µl H2OMQ were added ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 µl T4-PNK buffer (NEB), 2 µl T4-PNK (10 U/µl ...
-
bioRxiv - Biochemistry 2024Quote: Plasmids required for generating mutant strains were prepared using HiFi DNA assembly (NEB, USA) and then transformed into S ...
-
bioRxiv - Biochemistry 2024Quote: ... The DNA fragments for construction of the plasmids were obtained through either high-fidelity amplification using Q5 DNA Polymerase (NEB, USA) or restriction enzyme digestion ...
-
bioRxiv - Biochemistry 2024Quote: ... coli NEB511 were purchased from New England Biolabs (NEB). Headspace vials were purchased from Chemglass Inc.
-
bioRxiv - Biochemistry 2024Quote: ... NEBuilder HiFi DNA Assembly Master Mix (NEB) was used for subcloning ...
-
bioRxiv - Biochemistry 2024Quote: ... Methylated template DNA was digested using DpnI (NEB, Ipswich, MA, United States), before heat-shock transformation into chemically competent E ...
-
bioRxiv - Biochemistry 2024Quote: ... All cloning was performed using Gibson assembly cloning (NEB). All cDNA constructs were confirmed by sequencing.
-
bioRxiv - Biochemistry 2024Quote: ... and NEBStable (NEB) cells were used for molecular cloning ...
-
bioRxiv - Biochemistry 2024Quote: All PCR was performed with Q5 High-Fidelity DNA Polymerase kit (New England Biolabs) according to manufacturer’s protocols ...
-
bioRxiv - Biochemistry 2024Quote: ... we employed specific restriction enzymes (XhoI, NcoI, and NdeI from NEB) to cleave the plasmids at predetermined sites corresponding to the gene insertion ...
-
bioRxiv - Biochemistry 2024Quote: ... coli BL-21 cells (New England Biolabs) were used for the transformation of pET-28b(+ ...
-
bioRxiv - Bioengineering 2024Quote: ... dsDNA was mixed with lambda exonuclease (New England Biolabs, M0262) in the vendor supplied buffer ...
-
bioRxiv - Biochemistry 2024Quote: ... The strep tags were removed by incubating the proteins with 64 units of Enterokinase light chain (BioLabs) in 10 mM Tris ...
-
bioRxiv - Biochemistry 2024Quote: ... the larger DNA fragment of pNLX.Luc.R-.ΔAvrII73 following digestion with PflMI and AgeI restriction enzymes (New England Biolabs) was assembled with 1.85-kb synthetic fragments (Twist Bioscience ...
-
bioRxiv - Biochemistry 2024Quote: ... BsmBI-v2 0.5 µL (R0739L, New England Biolabs, Ipswich, Massachusetts, United States), pYTK001 0.5 µL ...
-
bioRxiv - Biochemistry 2024Quote: ... The on- and off-target genomic sites (experimentally determined from a previous study) were PCR amplified with Phusion plus DNA polymerases (New England Biolabs) and locus-specific primers having tails complementary to the Truseq adapters ...
-
bioRxiv - Biochemistry 2024Quote: ... T4 ligase 0.5 µL (M0202L, New England Biolabs, Ipswich, Massachusetts, United States), BsaI-HF®v2 0.5 µL (R3733L ...
-
bioRxiv - Biochemistry 2024Quote: ... BsaI-HF®v2 0.5 µL (R3733L, New England Biolabs, Ipswich, Massachusetts, United States), DNA parts 0.5 µL (20 fm) ...
-
bioRxiv - Biochemistry 2024Quote: ... cells were harvested 3 days after electroporation and genomic DNA was extracted with 100 μL lysis buffer (10 mM Tris-HCl, pH 7.5, 0.05% SDS, 25 μg/mL proteinase K (NEB)) at 37 °C with incubation for 1 hour ...
-
bioRxiv - Biochemistry 2024Quote: ... T4 ligase 0.5 µL (M0202L, New England Biolabs, Ipswich, Massachusetts, United States), BsmBI-v2 0.5 µL (R0739L ...
-
bioRxiv - Biochemistry 2024Quote: ... Template DNA was removed by treatment with DpnI (New England Biolabs), and the PCR products were subsequently 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs ...
-
bioRxiv - Biochemistry 2024Quote: ... PCR reactions were carried out using Taq polymerase (New England Biolabs) and contained 2.5 µM gene-specific primers that flanked the intron ...
-
bioRxiv - Biochemistry 2024Quote: ... The PCR product was purified by phenol chloroform extraction and RNA was synthesized using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). 1 ug of starting linear DNA template was used and transcription was performed for 2hrs at 37°C ...
-
bioRxiv - Biochemistry 2024Quote: ... and the PCR products were subsequently 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs) and self-ligated by T4 DNA ligase (New England Biolabs ...
-
bioRxiv - Biochemistry 2024Quote: ... and 20U RNase inhibitor (NEB). The translation reactions were incubated in 384 well plates at 30°C for 1hr ...
-
bioRxiv - Biochemistry 2024Quote: ... Parental plasmids were digested by adding 5 µL 10x cutsmart buffer (NEB), and 1 µL DpnI (NEB ...
-
bioRxiv - Biochemistry 2024Quote: ... A colony PCR using Taq Polymerase (New England Biolabs) on positive resistant colonies can help identifying false positives for Ampicillin resistance ...
-
bioRxiv - Biochemistry 2024Quote: ... coli (Suppl. Table 1) were cloned into a pBAD24 with ampicillin resistance using the USER cloning method (New England Biolabs, Table 2). All sequences were cloned including a 10x histidine tag located in the N-terminus for BtuG1-3 and the C-terminus for BtuG2 and all its mutants ...
-
bioRxiv - Biochemistry 2024Quote: ... reactions were quenched on ice with STOP Buffer (0.4 U proteinase K (NEB), 18 mM EDTA ...
-
bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted using the Monarch Total RNA Miniprep Kit (T2010S, New England Biolabs) and cDNA was synthesized using the SuperScript IV First-Strand Synthesis System (18091050 ...
-
bioRxiv - Biochemistry 2024Quote: Ribosome-protected fragments were dephosphorylated using T4 Polynucleotide Kinase (M0201, New England Biolabs). Dephosphorylated RNA was extracted using ROTI Acqua P/C/I for RNA extraction (X985.1 ...
-
bioRxiv - Biochemistry 2024Quote: ... the collected cell pellets were processed using Monarch RNA extraction kit (New England BioLabs). The RNA samples were then quantified with nanodrop and normalized before cDNA synthesis (Lambda Biotechnologies) ...
-
bioRxiv - Biochemistry 2024Quote: ... Libraries were PCR amplified using Phusion DNA Polymerase (M0530, New England Biolabs). Quality assessment of the libraries and sequencing were performed by the NGS platform of the University of Bern.
-
bioRxiv - Biochemistry 2024Quote: ... RNA was ligated using T4 RNA ligase 2 (M0242, New England Biolabs) to a preadenylated linker and purified with ROTI Aqua P/C/I for RNA extraction and precipitated with NaOAc ...
-
bioRxiv - Biochemistry 2024Quote: ... The plasmids were transformed into either BL21(DE3) (NEB) or OrigamiB(DE3 ...
-
bioRxiv - Biochemistry 2024Quote: ... Fragments were assembled into the pBAD24 vector using HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA) with 1-hour ...
-
bioRxiv - Biochemistry 2024Quote: ... followed by affinity-purification with amylose resin (New England Biolabs). The amylose eluates were purified further by anion exchange chromatography using Source 15Q Resin (Cytiva) ...
-
bioRxiv - Biochemistry 2024Quote: ... The HDV sequence was cloned into the XbaI/EcoRV sites of the AVA421 vector to enable linearization for run-off transcription by digestion with EcoRV-HF (NEB) in a reaction containing 1X CutSmart buffer and 0.4 U/μl EcoRV-HF.
-
bioRxiv - Biochemistry 2024Quote: ... a pET-derived vector that is normally used for T7 RNA polymerase-dependent protein expression (AVA421) was linearized by digestion with XbaI (NEB) in a reaction containing 1X CutSmart buffer and 0.5 U/μl XbaI to be used for run-off transcription using T7 RNA polymerase to transcribe the first 26 nucleotides following the T7 promoter sequence to generate the standard model RNA substrate ...
-
bioRxiv - Biochemistry 2024Quote: ... and 1 µL DpnI (NEB) directly to the PCR reaction mixture after PCR and continued incubation for 1h at 37°C ...
-
bioRxiv - Biochemistry 2024Quote: ... coli NEB C2566 cells (New England Biolabs). The cells were grown at 37°C in TB medium for HINT1 ...
-
bioRxiv - Bioengineering 2024Quote: ... and mCherry inserts were generated via PCR and cloned into the linearized viral backbone as a single fusion protein using HiFi cloning mix (NEB). H2B-iRFP nuclear marker was (Addgene Plasmid #90237) ...
-
bioRxiv - Biophysics 2024Quote: ... coli SHuffle T7 Express (New England Biolabs, Ipswich, MA) cells were transformed with 100 ng pET32-Trx-LqhαIT and ...
-
bioRxiv - Biophysics 2024Quote: ... Monarch RNase A (NEB) (0.1 ng/mL in final concentration ...
-
bioRxiv - Biochemistry 2024Quote: ... ExoIII (NEB#M0206V), APE1 (NEB#M0282S) ...
-
bioRxiv - Biochemistry 2024Quote: ... 1 µL T4 DNA Ligase (NEB), 100 ng DNA (oligo 3) ...
-
bioRxiv - Biochemistry 2024Quote: ... and LbCas12a (NEB#M0653S) were bought from New England Biolabs (Ipswich ...