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Citations for New England Biolabs :
3001 - 3050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... washed and incubated with T cell medium containing 5 μM (Snap-Cell Block S9106S, New England Biolabs, NEB) for 30 min at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... and A-tailed using the NEB Next Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Linkers were then ligated to the DNA using the NEB Next Ultra II Ligation Master Mix and Ligation Enhancer (New England Biolabs) ...
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bioRxiv - Bioengineering 2024Quote: ... Linkers were then ligated to the DNA using the NEB Next Ultra II Ligation Master Mix and Ligation Enhancer (New England Biolabs). The CAST-seq PCR reactions were performed in forward and in reverse in respect to the cleavage site ...
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bioRxiv - Bioengineering 2024Quote: ... barcoded with NEB Next Multiplex Oligos for Illumina (New England Biolabs) and sequenced by Illumina MiSeq (MiSeq Reagent Kit v3 600-cycle) ...
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bioRxiv - Bioengineering 2024Quote: ... amplified with Q5® Hot Start 2x Master Mix (New England Biolabs, Ipswich, Massachusetts, US). Target DNA was purified with a PureLink® PCR cleanup kit (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... along with 1x NEB 3.1r buffer (New England Biolabs). Cas9/Cas12a preformed RNP was a positive control and AuNP samples at various stages were added at 5 ug Au core ± BME (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: DNA cutting assays were performed following New England Biolabs (NEB) method.39,40 Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA was extracted using full B2M locus primers (Supplement Table 1) and amplified with Q5® Hot Start 2x Master Mix (New England Biolabs, Ipswich, Massachusetts, US). The target DNA was purified by PureLink® PCR cleanup (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... and tRNAGln with 5 nt-long 5’ leader and same 24 nt-long 3’ trailer as tRNATyr (5–tRNAGln–24) – were transcribed in reactions containing 1x T7 RNA polymerase reaction buffer (NEB), 0.001% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... coli PPIase (NEB), 5 U µL-1 T7 RNA polymerase (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... coli Shuffle T7 Express cells (C3029J, New England Biolabs) to aid disulfide bond formation ...
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bioRxiv - Biochemistry 2024Quote: ... library indexing was carried out with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Cat. E7645S) and sequenced on a NextSeq 1000 P2 cartridge (Illumina ...
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bioRxiv - Biochemistry 2024Quote: ... 5 U µL-1 T7 RNA polymerase (NEB) and 0.2 µg µL-1 template DNA ...
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bioRxiv - Biochemistry 2024Quote: ... Targeted regions were amplified using Q5 High-Fidelity DNA polymerase (New England Biolabs) with target-specific primers containing NGS barcodes ...
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bioRxiv - Biochemistry 2024Quote: ... then subsequent addition of 4 units of proteinase K (New England Biolabs) and incubation at 55 °C for 30 min ...
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bioRxiv - Biochemistry 2024Quote: The 1D4 purified receptor was mixed with purified jsGiq or hGi1 heterotrimer at a molar ratio of 1:1.25 and supplemented with apyrase (New England Biolabs) (25 mU/mL ...
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bioRxiv - Biochemistry 2024Quote: ... 100 ng of template DNA was used in a 2 hour in vitro transcription reaction using the HiScribe T7 High Yield RNA (New England Biolabs, Cat. E2040S) kit according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were further quenched by the addition of 0.2 ug of RNAse A (New England Biolabs) and incubation at 37 °C for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 NICO DE3 (New England Biolabs) chemically competent cells for expression ...
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bioRxiv - Biochemistry 2024Quote: ... Cleavage reactions were incubated at 37°C for 1 hour and subsequently worked up with 0.2 mg/mL RNase A (New England Biolabs) and 4 units of Proteinase K (New England Biolabs).
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bioRxiv - Biochemistry 2024Quote: ... E.coli BL21 (D3) strains (New England Biolabs) were transformed with a T7-driven effector plasmid and an E.coli constitutive promoter J23119 to drive sgRNA expression ...
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bioRxiv - Biochemistry 2024Quote: ... PURExpress-derived protein was mixed in a reaction containing 1x NEBuffer 2.1 (New England Biolabs), three-fold diluted PURExpress-derived protein ...
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bioRxiv - Biochemistry 2024Quote: ... coli constitutive ribosomal promoter J23119 with a poly-T region directly downstream of the sgRNA sequence for transcription termination using KLD mutagenesis (New England Biolabs). pBR322 and pET28 expression plasmids were co-transformed into E ...
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bioRxiv - Biochemistry 2024Quote: ... For the in-vitro transcription and translation reactions in PURExpress (New England Biolabs), nuclease PCR products were added at 5 nM final concentration and expressed at 37°C for 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Recovered cleavage products were blunted via Klenow fragment and dNTPs (New England Biolabs) to prepare them for adapter ligation ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were reverse transcribed with Induro Reverse Transcriptase (New England Biolabs, Cat. M0681L) for 30 minutes at 55°C using the appropriate reverse primer for each library according to the manufacturer protocol ...
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bioRxiv - Biochemistry 2024Quote: ... in vitro transcription templates were prepared via 8 cycles of PCR using 2x Q5 Master Mix (New England Biolabs, Cat. M0492S) with a T7 promoter containing forward primer as previously described ...
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bioRxiv - Biochemistry 2024Quote: ... and 4 units of Proteinase K (New England Biolabs).
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bioRxiv - Biochemistry 2024Quote: ... linearized with NdeI and XhoI (NEB), using HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... Cloning reagents were purchased from New England BioLabs (NEB). Codon-optimized gene fragments were purchased from Twist Biosciences ...
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bioRxiv - Biochemistry 2024Quote: A Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) was used to introduce mutations in amino acid residues predicted to play a role in stabilizing zinc ions ...
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bioRxiv - Biochemistry 2024Quote: ... using HiFi DNA Assembly Master Mix (NEB) following the manufacturer’s instructions (Supplementary Table 3) ...
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bioRxiv - Biochemistry 2024Quote: Fab Fragments were generated by taking .5 mg of IgG and digesting with 2 µL of Lys C (NEB#P8109S) at 37℃ ...
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bioRxiv - Biochemistry 2024Quote: ... Correct constructs were re-transformed into 10-beta chemically competent cells (NEB), from which clones were used to prepare glycerol stocks (25% glycerol ...
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bioRxiv - Biochemistry 2024Quote: ... coli cells (New England Biolabs) was inoculated into 1L of LB medium and induced with 0.1mM IPTG at 18C for 18hours ...
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bioRxiv - Biochemistry 2024Quote: Sequencing libraries for each sorted bin were generated by two-step PCR using a Phusion® DNA Polymerase (NEB) kit ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Sequencing libraries were generated using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA), following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µg of genomic DNA was digested with one of few combinations of restriction enzymes (Table S5, all enzymes from NEB, Ipswich, MA). Digested genomic DNA was ligated using T4 ligase (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were incubated with pre-washed Amylose resin (NEB) and incubated with gentle rotation for 2 hours at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... All mutangenesis were performed by Q5 Site-Directed Mutagenesis Kit (NEB; E0554S). For pCAG-OSF-FOXA1 (N-terminus 2xStrep-Flag-tagged ...
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bioRxiv - Biochemistry 2024Quote: ... were PCR amplified with indicated primers (see Table S1) and inserted into backbone plasmids via restriction digest and ligation (all enzymes from NEB) or Gateway cloning (Thermo) ...
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bioRxiv - Biochemistry 2024Quote: ... Digested genomic DNA was ligated using T4 ligase (NEB, Ipswich, MA) at 16°C for 16 hr ...
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bioRxiv - Biochemistry 2024Quote: ... and 10 U T4 polynucleotide kinase (New England Biolabs) in a final volume of 10 µL ...
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bioRxiv - Biochemistry 2024Quote: ... All truncated or tagged versions of clones were generated using Q5 high-fidelity DNA polymerase (New England Biolabs #M0515) and NEB-Gibson Assembly (New England Biolabs #E2611S) ...
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bioRxiv - Biochemistry 2024Quote: ... The vector was digested with PmeI and XbaI restriction enzymes (New England Biolabs). The inserts for pmirGLO miR144 and pmiRGLO miR31 plasmids were purchased from Integrated DNA Technologies (Table S5 ...
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bioRxiv - Biochemistry 2024Quote: ... 0.2 unit/mL yeast inorganic pyrophosphatase (New England Biolabs) (36) ...
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bioRxiv - Cancer Biology 2024Quote: ... or BsmBI (NEB, #R0739S). All plasmids were verified by sequencing and deposited with Addgene (#214678–214692) ...
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bioRxiv - Cancer Biology 2024Quote: ... and introduced by In Fusion cloning into pDual_dsCas9_Venus/BFP/Puro/Blast linearized with SbfI (NEB, #R0642S) or BsmBI (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.025 U/ml Taq polymerase (NEB, #M0267) in a final reaction volume of 15 µl and published thermal cycling parameters [17] ...
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bioRxiv - Cancer Biology 2024Quote: ... Double-stranded cDNA was then synthesized by reverse transcription and underwent end-repair and ligation using New England Biolabs (NEB) adapters ...