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Citations for New England Biolabs :
2951 - 3000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Libraries for sequencing were prepared by using NEB-Next end repair module (NEB, cat. E6050), followed by Klenow fragment exo-A-tailing (NEB ...
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bioRxiv - Plant Biology 2024Quote: 5 μl of ribosomal depleted RNA from each sample were used to prepare 64 individually barcoded RNA-seq libraries using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: Whole Methylome Sequencing (WMS) was performed on genomic libraries prepared using the NEBNext Enzymatic Methyl-seq Kit (New England BioLabs) following the manufacturer instructions ...
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bioRxiv - Systems Biology 2024Quote: ... The resulting full-length cDNA was gel-purified and ligated to the 5′ adaptor OWG920 with High Concentration T4 RNA Ligase (NEB M0437M) overnight on a shaking thermomixer ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli cells (New England Biolabs). Plasmids were validated by Sanger sequencing.
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bioRxiv - Plant Biology 2024Quote: ... Sequence fragments of ATML1 (START1, START2, and didomain) were generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with the following primers ...
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bioRxiv - Plant Biology 2024Quote: ... Site-directed mutagenesis transgenic plants were generated by using the Q5 ® Site-Directed Mutagenesis Kit (New England Biolabs) in the pDONR221 vector and recombined with the above-mentioned promotor constructs into the pFR7m24GW vector using the Gateway Technology ...
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bioRxiv - Systems Biology 2024Quote: ... The pool RNA was then gel-purified and capped using the Vaccinia Capping System (NEB M2080S). For co-transcriptional capping ...
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bioRxiv - Plant Biology 2024Quote: ... and PE 2.0 (5′-CAA GCA GAA GAC GGC ATA CGA GAT CGG TCT CGG CAT TCC TGC TGA ACC GCT CTT CCG ATC* T-3′) for 15 cycles using Phusion polymerase (NEB M0530S). The library was purified by electrophoresis on a 1.2% agarose gel to get rid of adapter dimers ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was A-tailed with Fermentas Klenow 3′ to 5′ exonuclease (Cat# EP0421) and ligated with adaptor oligonucleotides (NEB NEXT adaptor oligos) using Mighty Mix Ligase (Cat# TAK6023) ...
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bioRxiv - Genomics 2024Quote: ... circularized library is then used as a template for rolling circle amplification (RCA) using Phi29 (NEB) with a random hexamer primer for 18 hours at 30C then heat inactivated for 10 minutes at 65C ...
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bioRxiv - Physiology 2024Quote: ... cDNA was synthesized using a cDNA Reverse Transcriptase Kit (New England Biolabs). TaqMan-based real time quantitative polymerase chain reactions (qRT-PCR ...
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bioRxiv - Physiology 2024Quote: ... 20 mg of protein from each sample was subjected to PNGase F treatment (NEB #P0704) to remove N-linked oligosaccharides prior to SDS-PAGE ...
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bioRxiv - Genetics 2024Quote: ... coli (NEB cat no. C2992H) and resulting bacterial colonies used to inoculate 50 mL of LB and grown at 37°C for 6 h at 250 rpm in an orbital shaker ...
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bioRxiv - Biochemistry 2024Quote: ... The lysates were then incubated with 80 μL streptavidin magnetic beads (New England Biolabs) at 4°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Strep-GRP94 FL was cloned from C2C12 cDNA into pET21d by Gibson assembly (NEB Hifi Builder). His6-BiP-NBD (27-411 ...
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bioRxiv - Biochemistry 2024Quote: ... The activating Q163C/Q309C cysteine mutations were introduced into αMI-domain using the Q5 Site-Directed Mutagenesis Kit (NEB). The plasmids were transformed into either BL21(DE3 ...
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bioRxiv - Biochemistry 2024Quote: Point mutants were generated using inverse polymerase chain reaction (PCR) with Phusion DNA polymerase (New England Biolabs) or Herculase II (Agilent) ...
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bioRxiv - Biochemistry 2024Quote: Plasmid constructs were cloned by Gibson assembly(51) using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Codon-optimised gene fragments for wild-type MxEnc and the triple mutants 3×His-MxEnc ...
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bioRxiv - Biochemistry 2024Quote: ... and self-ligated by T4 DNA ligase (New England Biolabs) before being used to transform Top10 competent cells (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2024Quote: ... All PCRs were performed using LongAmp Taq DNA polymerase (NEB, cat no. 50994936) in a 25 μL reaction volume according to manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... M13KO7 helper phage (New England Biolabs N0315S) was added to each culture (final concentration of 1 x 108 pfu/mL ...
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bioRxiv - Genetics 2024Quote: ... ssDNA was isolated the following day per M13KO7 helper phage standard manufacturer protocol (NEB cat no. N0315S).
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bioRxiv - Immunology 2024Quote: ... and PCR amplified with NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs). Following purification with the PCR Purification Kit (QIAGEN) ...
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bioRxiv - Developmental Biology 2024Quote: ... cutting efficiency was determined by T7-endonuclease assay (T7 Endonuclease I, New England Biolabs, M0302) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... NEBnext dual indices (NEB #E7600S) were added to the amplicons using NEBNext® Ultra™ II Q5® Master Mix (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... All clones were subjected to DpnI (New England Biolabs Inc.) digestion followed by transformation ...
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bioRxiv - Biochemistry 2024Quote: ... containing plates and checked by the consequent release of correct-sized fragments following double digestion with NdeI and BamHI (New England Biolabs Inc.). All the clones were confirmed by sequencing.
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bioRxiv - Cell Biology 2024Quote: ... coli BL21 (DE3) (C2527I, New England Biolabs) and grown in 1 L of Terrific Broth (TB ...
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bioRxiv - Cell Biology 2024Quote: ... NEB)/EcoRI-HF (Cat #: R310S; NEB) digestion and subsequent ligation (Cat # ...
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bioRxiv - Cell Biology 2024Quote: ... was used to generate pcDNA3.1-ABDTS from pcDNA3.1 vector digested with EcoRI-HF (Cat #: R310S; NEB)/NotI-HF (Cat # ...
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bioRxiv - Cell Biology 2024Quote: ... digestion and subsequent ligation (Cat #: M0202S; NEB). All newly generated constructs were verified by DNA sequencing (GENEWIZ from Azenta).
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bioRxiv - Cell Biology 2024Quote: ... Gibson Assembly (with Gibson Assembly Master Mix, E2611S; NEB, Ipswich, MA) was used to generate pcDNA3.1-ABDTS from pcDNA3.1 vector digested with EcoRI-HF (Cat # ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3.1-ABDTL was generated via PCR from pcDNA3.1-ABDTS and inserted into pcDNA3.1 via BamHI-HF (Cat #: R3136S; NEB)/EcoRI-HF (Cat # ...
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bioRxiv - Cell Biology 2024Quote: ... NEB)/NotI-HF (Cat #: R3189S; NEB,) and the following three fragments containing complementary regions ...
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bioRxiv - Neuroscience 2024Quote: ... and then restriction digested using HindIII and PspXI (New England BioLabs, Ipswich, MA) to create compatible sticky ends ...
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bioRxiv - Developmental Biology 2024Quote: ... Fragments were digested using EcoRI-HF (New England Biolabs) and purified using the MinElute Cleanup kit (Qiagen) ...
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bioRxiv - Developmental Biology 2024Quote: ... pUAST vector was double digested using EcoRI-HF and HpaI (New England Biolabs), then the digested vector was run in an electrophoresis gel ...
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bioRxiv - Developmental Biology 2024Quote: The mRNA isolation utilized the NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB, #E7490) with 1 μg of total RNA ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA-seq libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, #E7420L), followed by sequencing on an Illumina Hiseq 4000 system ...
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bioRxiv - Cell Biology 2024Quote: ... under a CamKII promoter were both restriction digested with AgeI and the HALO protein was ligated with Quick Ligase (NEB) into the ER-GCaMP yielding the CamKII ER-Halo-GCaMP6-150 ...
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bioRxiv - Cell Biology 2024Quote: ... The ER-Halo-GCaMP6-150 was PCR amplified and the resulting fragment was combined with the hDLXI56i backbone with HiFi (NEB). The hDLXI56i enhancer was originally obtained from CN1851-rAAV-hI56i-minBglobin-iCre-4×2C-WPRE3-BGHpA (Graybuck et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... Total RNA was then purified with Monarch RNA Cleanup Kit (NEB) and quantified using Qubit Fluorimetric Quantification (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... The sorted spermatid fractions were collected in Monarch DNA/RNA protection reagent (NEB) and flash frozen in liquid nitrogen before the RNA extraction.
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bioRxiv - Developmental Biology 2024Quote: ... The sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB Catalog# E7645S) following manufacturer instructions.
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bioRxiv - Developmental Biology 2024Quote: ... The amplified pools were subjected to PCR product purification by Monarch PCR and DNA Cleanup Kit (NEB). The purified cDNAs were cloned by NEB PCR Cloning Kit and individual clones were isolated and sequenced (Azenta Life Sciences).
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bioRxiv - Developmental Biology 2024Quote: Total RNA was extracted from collected spermatids fractions with Monarch RNA extraction kit (NEB) according to manufacturers’ protocol for RNA extraction from tissue or leukocytes ...
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bioRxiv - Developmental Biology 2024Quote: ... 400 µL MNase complete buffer (85 mM Tris-HCl pH 7.5, 3mM MgCl2, 2mM CaCl2, 0.3M Sucrose, 60 IU of MNase (NEB Catalog# 0247S) was added to each chromatin preparation ...
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bioRxiv - Developmental Biology 2024Quote: The Rapid Amplification of 5’cDNA end was carried out with Template Switching Reverse Transcription protocol (Wulf et al, 2019) provided by New England Biolabs (NEB #M0466). In the first step ...
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bioRxiv - Developmental Biology 2024Quote: ... The purified cDNAs were cloned by NEB PCR Cloning Kit and individual clones were isolated and sequenced (Azenta Life Sciences).