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Citations for New England Biolabs :
2901 - 2950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490, New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... This end-repaired DNA was subjected to A-tailing using the Klenow 3′–5′ exo− enzyme (New England Biolabs). The subsequent step involved the ligation of methylated adapters to the A-tailed DNA ...
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bioRxiv - Synthetic Biology 2024Quote: ... The resulting helper plasmids were transformed into DH5α competent cells (NEB, C2987H), yielding four individual helper cell lines (M13cp-dg1 ...
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bioRxiv - Plant Biology 2024Quote: ... and the genes were PCR-amplified using Q5® High-Fidelity 2X Master Mix (New England Biolabs).
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bioRxiv - Plant Biology 2024Quote: ... using T4 DNA Ligase (New England Biolabs). Following adapter ligation ...
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bioRxiv - Synthetic Biology 2024Quote: ... library concentrations were quantified with qPCR using the NEBNext Library Quant Kit for Illumina (NEB, E7630S), and pre- and post-selection libraries were combined as two pools ...
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bioRxiv - Synthetic Biology 2024Quote: ... and designed for library variant expression upon induction by IPTG—using NEBuilder Hifi DNA Assembly Master Mix (NEB, E2621L). All libraries were then precipitated with isopropanol ...
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bioRxiv - Synthetic Biology 2024Quote: ... transformed into electrocompetent DH5α cells (NEB, C2989K), and plated on 2XYT-carbenicillin-1% glucose ...
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bioRxiv - Synthetic Biology 2024Quote: ... or Q5 (New England Biolabs, Ipswich, Massachusetts, USA). The construction method for each plasmid built in this work can be consulted in Table S1 ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA was cloned using either classical restriction and ligation ([55]; restriction enzymes, T4 DNA ligase and Quick Ligation kit from New England Biolabs, Ipswich, Massachusetts, USA) or isothermal assembly ([56] ...
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bioRxiv - Cancer Biology 2024Quote: ... NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs) and Multiplex Oligos for Illumina Systems (Cell Signaling ...
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bioRxiv - Synthetic Biology 2024Quote: ... The area of the coverslips around the tissue slices was then dabbed dry with a Kimwipe before mounting a SecureSeal hybridization chamber (Grade Biolabs #621502) on top of the tissue slices ...
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bioRxiv - Synthetic Biology 2024Quote: ... Minipreps were performed using Monarch Plasmid Miniprep kit (New England Biolabs, Ipswich, Massachusetts, USA) and PCR products were purified with Monarch PCR and DNA Cleanup kit (New England Biolabs ...
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bioRxiv - Synthetic Biology 2024Quote: ... restriction enzymes and T4 DNA ligase from NEB and PCRs were done using the PrimeSTAR Max DNA polymerase from Takara Bio ...
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bioRxiv - Synthetic Biology 2024Quote: ... or isothermal assembly ([56]; Gibson Assembly MasterMix, New England Biolabs, Ipswich, Massachusetts, USA). Plasmid pBLAM1-2-TS2-msfGFP-mKate2 was subjected whole-plasmid site-directed mutagenesis[57] to eliminate MmeI site in the TetR gene ...
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bioRxiv - Cell Biology 2024Quote: ... Following BamHI (NEB #R3136)-XhoI (NEB #R0146) digest of the MDM2 PCR product and destination vector ...
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bioRxiv - Cell Biology 2024Quote: ... Following BamHI (NEB #R3136)-XhoI (NEB #R0146 ...
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bioRxiv - Cell Biology 2024Quote: ... following NdeI (NEB #R0111S)-XhoI (NEB #R0146) digest.
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bioRxiv - Cell Biology 2024Quote: ... following NdeI (NEB #R0111S)-XhoI (NEB #R0146 ...
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bioRxiv - Cell Biology 2024Quote: ... ligations were performed with T4 ligase (NEB #M0202). Ligated plasmids were transformed into competent XL-1 blue cells (Agilent Technologies #200249) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and PCR products were purified with Monarch PCR and DNA Cleanup kit (New England Biolabs, Ipswich, Massachusetts, USA).
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bioRxiv - Systems Biology 2024Quote: Approximately 100 ng of each RNA sample was used to create Illumina sequencing libraries using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... Approximately 100 ng of each DNA sample was used to create Illumina sequencing libraries using a NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs (NEB), E7805S) ...
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bioRxiv - Systems Biology 2024Quote: ... After PCR amplification by Phusion DNA polymerase (NEB) and TBS380 (Picogreen ...
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bioRxiv - Systems Biology 2024Quote: 50 ng RNA of each sample were reverse transcribed and barcoded by using the PCR-cDNA barcoding kit (SQK-PCB111.24) and NEBNext Companion Module (NEB E7180L). Libraries were then sequenced on the Nanopore PromethION platform.
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bioRxiv - Systems Biology 2024Quote: ... Approximately 100 ng of each DNA sample was used to create Illumina sequencing libraries using a NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs (NEB), E7805S) ...
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bioRxiv - Cancer Biology 2024Quote: ... coli (High Efficiency) (NEB, C2987H) and Stbl3 Competent E ...
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bioRxiv - Plant Biology 2024Quote: ... The sgRNA was first synthesised using the EnGen sgRNA synthesis kit New England Biolabs (NEB #E3322) before mixing with the Cas9-NLS to form the RNP complex ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting cDNA was then amplified using Phusion High-Fidelity DNA Polymerase (New England Biolabs). GAPDH level was utilized as the transcriptional control ...
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bioRxiv - Developmental Biology 2024Quote: ... runx1 (using pBS-runx1a, linearized with HindIII (R0104S, NEB), T7 polymerase for IVT)183 (a kind gift from Dr ...
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bioRxiv - Cancer Biology 2024Quote: ... was conducted using the Monarch® Genomic DNA Purification Kit (NEB, Cat # T3010S). Primers were procured from Integrated DNA Technologies and designed with the PrimerQuest™ Tool (IDT ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA fragments were then cloned into a lentiviral expression vector with a Gibson assembly product (NEB, E2611S). DNA sequencing was performed to confirm accuracy of the vector ...
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bioRxiv - Cancer Biology 2024Quote: ... Bound antibodies were detected by incubation with DyLight 800-conjugated secondary antibodies (New England BioLabs), and analysed using an InnoScan 710-IR scanner (Innopsys) ...
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed using the NEBNext Ultra II Library Prep Kit for Illumina (New England BioLabs, #E7645S) per manufacturer’s instructions with minor modifications ...
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bioRxiv - Zoology 2024Quote: ... PCR products were purified with a Monarch PCR & DNA clean-up kit (New England Biolabs, Whitby, ON, Canada) and used as template to reamplify the products to incorporate a Kozak translation initiation sequence prior to the start codon (Kozak ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... IDT synthesized oligonucleotides were annealed and phosphorylated using PNK (NEB#C3019) at 37° C for 30min ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... and subsequently transformed into NEB10beta (NEB#C3019). Purified DNA from propagated colonies were confirmed for gRNA insertion via Sanger sequencing (Genewiz) ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... in an all-in-one T7 ligase (NEB#M0318S) based Golden-Gate assembly (37° C for 5min ...
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bioRxiv - Microbiology 2024Quote: ... Purified RNA was depleted of ribosomal RNA (rRNA) using a NEBNext® rRNA Depletion Kit (Bacteria) (NEB, #E7850L/X) and library preparation was done using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Murine (NEB, #M0314S/L) and nuclease-free water at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... or Phusion (NEB) polymerases according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Specific mutations in human PSEN1 were obtained by Q5 site-directed mutagenesis of the pMSCV-puro vector containing wild type human PSEN1.26 We designed primers for Q5 site-directed mutagenesis (New England Biolabs) with NEBaseChanger.neb.com and performed site-directed mutagenesis according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... which contained 5mM Na3VO4 (#P0758S, New England Biolabs) and a protease inhibitor (cOmplete ...
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bioRxiv - Cancer Biology 2024Quote: ... rRNA was depleted from total RNA using the rRNA depletion kit (NEB #E6310). After fragmentation of the rRNA reduced RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Dot blot samples were prepared from 1 µg gDNA with or without RNaseH1 2500 U treatment (NEB, M0297) O/N 37°C.Samples were denatured in Denaturation Buffer (NaOH 400 mM ...
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bioRxiv - Neuroscience 2024Quote: ... Illumina library was made from polyA RNA with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s manual74 ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using unique dual index primer pairs (NEBNext Multiplex Oligos for Illumina from New England BioLabs, # E6440S) according the following parameters ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by Klenow fragment exo-A-tailing (NEB, cat. M0212), adaptors ligation with Quick Ligation kit (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... adaptors ligation with Quick Ligation kit (NEB, cat. M2200) and amplification with Phusion Flash HF PCR master mix (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA was extracted in native conditions and digested by restriction enzyme cocktail fragmentation (BsrGI-HF NEB cat. R3575, EcoRI-HF NEB cat. R3101, HindIII-HF NEB cat ...