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Citations for New England Biolabs :
2851 - 2900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... the ExoI reaction mixture containing 1 x ExoI buffer and 1 U/μL ExoI (NEB) enzyme was prepared ...
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bioRxiv - Microbiology 2024Quote: ... truncated K227Q (NEB) was prepared on ice ...
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bioRxiv - Microbiology 2024Quote: ... in 1 x rCutSmart buffer (NEB) at 37 °C for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 U/µL T4 RNA ligase 1 (NEB) and 10 U/µL T4 RNA ligase 2 ...
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bioRxiv - Microbiology 2024Quote: ... Polyadenylated RNA was then isolated from the whole-cell RNA using NEB Oligo d(T)25 Magnetic beads (NEB, S1419S) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2024Quote: ... Samples were DNase I (NEB, M0303) treated for 20 mins at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... 200U/ml murine RNase inhibitor (NEB, M0314)) ...
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bioRxiv - Microbiology 2024Quote: ... The backbone fragments were generated using Q5 high fidelity polymerase (NEB, M0491), treated overnight with DpnI and the DNA was purified using MicroElute Cycle-Pure Kit (Omega Bio-Tek) ...
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bioRxiv - Microbiology 2024Quote: ... L742A and L786A mutations were inserted into pACEBac1-Strep-Psc-BICD2711-800 (either individually or in combination) by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB). The full-length mouse BICD1 sequence (UniProt Q8BR07 ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM DTT and 100 μM ATP) and incubated with 10 μM SNAP-Cell TMR-Star dye (NEB) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... A fragment containing the CRISPR array plus the extra BsrG1 restriction site was cut from the synthesized pUC19 by digestion with DraI and BstZ17I enzymes and cloned into the BsaI-linearized pSTU-1 plasmid using the NEBuilder HiFi cloning kit (New England Biolabs, UK). The plasmid sequence was confirmed by sequencing (Eurofins Genomics ...
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bioRxiv - Microbiology 2024Quote: ... His- tagged proteins were purified by NEBExpress® Ni-NTA Magnetic Beads (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... were digested with NotI and the wc-1 and wc-2 fragments were cloned into the respective plasmids with NEB HiFi DNA assembly mastermix (NEB, US). This resulted in the prey plasmid wc-1-pMB29 and bait plasmid wc-2-pMB28 ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of 10x r3.1 buffer (NEB), 2 µl of 100µM DTT and 1 µl of NudC (M0607S ...
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bioRxiv - Microbiology 2024Quote: ... PCR product was then directly used for circularization with KLD enzyme mix (NEB) according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... and the RNA was treated with DNase (New England Biolabs). Next ...
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bioRxiv - Microbiology 2024Quote: ... After restriction digest with the respective restriction endonucleases (NEB, USA) (supplementary Table 4) ...
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bioRxiv - Microbiology 2024Quote: Approximately 40mg of feces were used for RNA extraction using the Monarch® Total RNA Miniprep Kit (New England Biolabs, NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 μg of this amplified DNA (hereto referred as wga DNA) was digested by 1.5 μL of T7 endonuclease (New England Biolabs, Ipswich, MA) in a 50 μL reaction for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... Recorded data were further processed using CGQquant (Aquila Biolabs) for merging the replicates ...
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bioRxiv - Microbiology 2024Quote: ... the purified PCR products were ligated with the empty vectors in a molar ratio of 1:4 using T4 DNA ligase (NEB, USA) at 16 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR products were then assembled in an ApaI and SacII digested pSVA407 vector via Gibson Assembly following the manufacturer’s instructions (New England Biolabs). The respective deletion mutants were then transformed with the resulting knock-in plasmids pSVA407-ΔupsE:P1117 saci_1117 CtSS and pSVA407-ΔupsE:P2033 saci_2033-2034 ...
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bioRxiv - Microbiology 2024Quote: ... 7.5µl 2x NEBNext High Fidelity Master Mix (NEB), and 1.25µl of each barcoded Nextera N5x and N7x index primers per reaction ...
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bioRxiv - Microbiology 2024Quote: ... primers oAA033-034 were used to remove mprF using the Q5 site-directed mutagenesis kit (NEB Cat. No. E0554S) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and 1µl 100mM dNTPs (NEB), plus 100ng of the pDNA and UltraPure water ...
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bioRxiv - Microbiology 2024Quote: ... 50 μg RNA was treated with T4 polynucleotide kinase (New England Biolabs, MA, USA) according to the manufacturer’s instructions at 37°C ...
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bioRxiv - Microbiology 2024Quote: SV40 enhancer was amplified with PCR using Q5 High-Fidelity DNA Polymerase (NEB) from plasmid template ...
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bioRxiv - Microbiology 2024Quote: ... Cells were treated with 200 U/mL of α2,3,6,8,9 neuraminidase A (NEB Bioke, P0722L) and 0.6 U of α1,2,3,4,6-L-fucosidase (Megazyme ...
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bioRxiv - Microbiology 2024Quote: SV40 LT area was amplified with PCR using Q5 High-Fidelity DNA Polymerase (NEB). Template was SV40 genome WT4 plasmid which was received as a gift from James DeCaprio ...
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bioRxiv - Microbiology 2024Quote: ... gel purified with Monarch DNA Gel Extraction Kit (NEB), and ligated with T4 DNA ligase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... PCR product and pSIP409 plasmid were digested with NcoI and XhoI enzymes (NEB), gel purified with Monarch DNA Gel Extraction Kit (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... Taq 2X Master Mix (New England Biolabs) was used for the reaction and PCR primers are listed in Supplementary Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... DNA was PCR-amplified using Phusion polymerase (New England Biolabs; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA was PCR-amplified using Phusion polymerase (New England Biolabs; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Restriction enzymes and T4 DNA ligase were purchased from NEB. M ...
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bioRxiv - Microbiology 2024Quote: ... Ligations were transformed into NEB10β competent cells following the manufacturer recommendations (New England Biolabs). Correct plasmids were verified by PCR and sanger sequencing ...
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bioRxiv - Microbiology 2024Quote: Plasmids were constructed by amplifying inserts by PCR using specific oligonucleotides and the Q5 high fidelity enzyme (New England Biolabs). PCRs were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Microbiology 2024Quote: ... we used a similar protocol but library preparation was performed using the NEBNext Ultra DNA Library Prep Kit (New England BioLabs, ref. E7370) for Illumina following manufacturer’s specifications except for end repair ...
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bioRxiv - Microbiology 2024Quote: ... or Restriction Free cloning (van den Ent and Löwe 2006) and site-directed mutations were introduced by the method of Stratagene QuikChangeTM using Q5 polymerase (NEB). In experiments relating to promoter repression ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were cleaned using the Monarch PCR & DNA cleanup kit (NEB, T1030L). VSG sequences were determined by amplicon nanopore sequencing performed by Plasmidsaurus using Oxford Nanopore Technology with custom analysis and annotation or Sanger sequencing with the sequencing primer (5’-AGAGAATACTAAGCTAGTTGGC-3’ ...
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bioRxiv - Microbiology 2024Quote: ... Lister427 parasites were electroporated with pHH-HYG-VSG plasmids digested with BamHI-HF (NEB, R3136S). After 16-24 hours recovery ...
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bioRxiv - Microbiology 2024Quote: ... digested with NotI-HF (NEB, R3189S). Parasites were immediately selected in 5ug/mL Blasticidin (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The fragments were cloned into a pBSIIKS+ plasmid digested with SacI and BamHI (NEB) using Infusion (Takara ...
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bioRxiv - Microbiology 2024Quote: ... and in vitro transcription and polyadenylation was performed using HiScribe T7 ARCA mRNA Kit (with tailing) from NEB, following recommended procedure ...
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bioRxiv - Microbiology 2024Quote: ... extension 15s) genes for 30 cycles using OneTaq DNA Polymerase (NEB, M0480L).
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bioRxiv - Microbiology 2024Quote: ... sticky-end annealed sgRNAs were ligated into a BbsI-HF (NEB, R0539S) digestion of pT7sgRNA ...
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bioRxiv - Microbiology 2024Quote: ... An aliquot of 1 A260 unit of ribosome-bound mRNA fraction was digested with 800 units Micrococcal nuclease (NEB) for 20 min at 25°C ...
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bioRxiv - Microbiology 2024Quote: ... AnTat1.1 sequence was obtained from AnTat1.1 specific cDNA from mouse infection D6 cloned into a pMiniT vector with the PCR Cloning Kit (NEB, E1202S). VSG-228 was partially amplified from VSG PCR (see below ...
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bioRxiv - Microbiology 2024Quote: ... and prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manual ...
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bioRxiv - Microbiology 2024Quote: ... reactions were performed in a final volume of 100 μl using the PURExpress ΔRibosome In vitro Protein Synthesis Kit (New England Biolabs) according to the manufacturer instructions and with ribosomes purified from E ...