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Citations for New England Biolabs :
2801 - 2850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... 5 μl of CutSmart buffer 10x (NEB), 5 μl of ATP 10 mM ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... The concatemers are further treated with T7 Endonuclease (NEB) to remove branches before being prepped for Nanopore sequencing.
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bioRxiv - Genomics 2024Quote: ... 5 μl of CutSmart buffer 10x (NEB), 5 μl of ATP 10 mM, ...
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bioRxiv - Genomics 2024Quote: ... PCR1 (UMI addition) was performed in a 50 μl reaction with Q5 Polymerase (NEB M0492S), with 2.5 μL of each 10 μM primer (oSP1008 and oSP997 ...
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bioRxiv - Genomics 2024Quote: ... 0.67x NEB buffer 4 (NEB, B7004S), 0.13% Triton X-100 (sigma ...
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bioRxiv - Genomics 2024Quote: ... gel-purified (NEB), and ligated to the annealed shRNA oligos ...
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bioRxiv - Genomics 2024Quote: ... Tet-puro-pLKO plasmid was digested with AgeI and EcoRI (NEB) restriction enzymes ...
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bioRxiv - Genomics 2024Quote: ... and 4000 U T4 DNA ligase (NEB). The ligation reaction was stopped by adding 0.5 M EDTA (final concentration 10 mM) ...
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bioRxiv - Genomics 2024Quote: ... Nuclei were digested using 800 U of BamHI (for 5’-5’ loop) or BglII (for junction loop) (NEB) on a shaker overnight at 37°C ...
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bioRxiv - Genomics 2024Quote: ... 2 µg genomic DNA was used to construct a sequencing library following the manufacturer’s instructions using NEBNext Ultra DNA Library Prep Kit (NEB Inc., America). Paired-end sequencing libraries with an insert size of approximately 400 bp were sequenced on an Illumina HiSeq 4000 sequencer ...
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bioRxiv - Genomics 2024Quote: ... The digested nuclei were washed and resuspended by 1 x T4 ligase buffer (NEB). Nuclei were then ligated for 4 h at room temperature by adding 1% Triton X-100 ...
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bioRxiv - Genomics 2024Quote: ... Coli (High Efficiency) cells (NEB) and incubated overnight at 37°C on ampicillin LB agar plates ...
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bioRxiv - Genomics 2024Quote: ... RNA was the digested by adding 0.5 µL each of RNAse A and RNAse H (ThermoFisher R1253 and NEB M0297L) to each reaction and incubating for 30 min at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... libraries were prepared using NEBNext Ultra II Library Prep Kit for Illumina (NEB), size selected (150-500 bp) ...
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bioRxiv - Immunology 2024Quote: ... DNA library construction was performed using the NEBNext Ultra II DNA Library Prep Kit from NEB (E7645L). The libraries were sequenced on Illumina NovaSeq 6000 system (50 bp paired-end ...
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bioRxiv - Genomics 2024Quote: ... and 1X BsmBI-v2 Golden Gate Enzyme Mix (New England Biolabs E1602S) with the thermal cycling protocol ...
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bioRxiv - Genomics 2024Quote: ... dephosphorylated with rSAP (New England Biolabs M0371L), and gel purified with the QIAquick PCR & Gel Cleanup Kit (Qiagen 28506) ...
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bioRxiv - Genomics 2024Quote: ... 1X T4 DNA ligase buffer (New England Biolabs M0202S), and 400 U/µL of T4 DNA ligase (New England Biolabs M0202S) ...
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bioRxiv - Genomics 2024Quote: ... Plasmid libraries were amplified using 1X NEBNext Ultra II Q5 Master Mix (New England Biolabs M0544), 1X EvaGreen dye (Biotium 31000) ...
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bioRxiv - Genomics 2024Quote: ... mixed with 50 µL 2x NEBuilder HiFi DNA assembly Master mix (NEB E2621L) and incubated at 50 °C for 2 hr ...
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bioRxiv - Genomics 2024Quote: ... Enzymatic digestion was performed using enzymes Taq1-v2 and Pst1-HF (New Englands Biolabs, 20assachusetts, USA), in agreement with the simulation results of Herry et al (2023) ...
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bioRxiv - Genomics 2024Quote: ... Nuclei were then resuspended in 500 μl of 1× NEBuffer3 (NEB) containing 0.3% SDS and incubated for 1 h at 37°C ...
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Recurrent loss of crossover interference punctuates the recombination landscape across yeast speciesbioRxiv - Genomics 2024Quote: DNA extraction was performed using the Monarch HWW DNA extraction kit (NEB) following the manufacturers protocol ...
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bioRxiv - Genomics 2024Quote: ... the reverse crosslinked nucleic acids were purified with Monarch RNA purification kit (NEB, 76307-460) and eluted into 21 µL of nuclease free water ...
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bioRxiv - Genomics 2024Quote: ... Small RNA sequencing libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina following the manufacturer’s recommendations with the 3’ ligation step changed to 16°C for 18 hours to improve capture of methylated small RNAs (New England Biolabs, cat# E7300S). Small RNA PCR amplicons were size selected on 10% polyacrylamide non-denaturing gels ...
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bioRxiv - Genomics 2024Quote: ... RNA from each sample was enriched from 1 µg total RNA by the NEBNext® Poly(A) mRNA Magnetic Isolation Module (Catalog #: E7490, New England Biolabs Inc.). RNA-Seq libraries were prepared using NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (Catalog # ...
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bioRxiv - Genomics 2024Quote: ... Libraries were then created from each fraction using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645L) and the Unique Dual Index NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Genomics 2024Quote: ... and the Unique Dual Index NEBNext Multiplex Oligos for Illumina (NEB, E6440S); final qualitative and quantitative controls were performed through an Agilent 2100 Bioanalyzer System and a Qubit 4.0 fluorometer ...
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bioRxiv - Genomics 2024Quote: ... Metagenomic libraries were generated with the NEBNext Ultra DNA Library Preparation kit following the manufacturer’s instructions (New England Biolabs, Ipswich, MA, United States) and paired-end sequenced (2 x 150 bp ...
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bioRxiv - Genomics 2024Quote: ... SphI and MluCI (New England Biolabs), and ligated to specific adapters (P1 and P2 ...
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bioRxiv - Genomics 2024Quote: ... DM and DM+DAM treated gDNA was digested with DpnI (NEB, USA), which selectively cleaves methylated GATC sites ...
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bioRxiv - Genomics 2024Quote: ... The DNA was treated with NEBNext Ultra II End Repair/dA-Tailing Module (NEB, USA) and NEBNext FFPE DNA Repair Mix (NEB ...
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bioRxiv - Genomics 2024Quote: ... The barcodes were ligated to samples using the NEBNext Ultra II Ligation Module (NEB, USA) after which the samples were purified and quantified ...
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bioRxiv - Genomics 2024Quote: ... and NEBNext FFPE DNA Repair Mix (NEB, USA), purified using Ampure XP (Beckmann Coulter ...
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bioRxiv - Genomics 2024Quote: ... The genomic DNA was incubated in a mix containing S-Adenosyl methionine (SAM) along with the DNA adenine Methyltransferase (dam, NEB, USA) and its corresponding buffer for 4 hours at 37°C ...
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bioRxiv - Genomics 2024Quote: ... we prepared two additional libraries with DNA extracts to which a DNA repair step was applied using the Sequential Reaction Protocol for PreCR Repair Mix (New England BioLabs) prior to the actual library preparation.
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bioRxiv - Genomics 2024Quote: ... and second-strand synthesis was performed using Bst 2.0 DNA polymerase (NEB cat. M0537) with an increasing temperature gradient of 15-31°C with shaking at 1750 rpm ...
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bioRxiv - Genomics 2024Quote: 1 μl of Cas9 nuclease (NEB) and 2 μl of single-guide RNA (check concentration ...
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bioRxiv - Microbiology 2024Quote: ... 1.25 µL native ligation barcode (ONT SQK-NBD114-96) and 3 µL Blunt/TA Ligase Master Mix (NEB). The reaction was mixed by pipetting ...
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bioRxiv - Microbiology 2024Quote: ... 30 µL of the barcoded DNA was ligated onto 2.5 µL Native Ligation Adapter (NA, ONT SQK-NBD114-96) by addition of 12.5 µL NEBNext Quick Ligation Reaction Buffer (NEB) and 5 µL high conc ...
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bioRxiv - Microbiology 2024Quote: ... T4 DNA Ligase (NEB). After incubation for 20 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... We then assembled the digested backbone with the mutagenized GPC inserts with NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621) at a 1:2 vector to insert ratio for a 1 hr reaction ...
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bioRxiv - Microbiology 2024Quote: ... and ASP14_lasA_KO_DN_F and ASP15_lasA_KO_SacI_DN_R (downstream) (Table S3) and cloned into pEXG2-Tc with Gibson assembly (New England Biolabs). pEXG2-Tc-ΔlasA (Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2L-O-methyltransferase (New England Biolabs). The mRNA was purified ...
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bioRxiv - Microbiology 2024Quote: ... coli NiCo21(DE3) (NEB BioLabs) was used to express recombinant proteins ...
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bioRxiv - Microbiology 2024Quote: ... The PCR amplifications were performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs) with supplementation with High GC-enhancer to the reaction following the manufacturer’s instructions and primers synthesized by Eurofins Genomics ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM MgCl2) and either 25 nM NudE.1 WT or NudE.1 E64,65Q or NudC WT (NEB). For NAD spike-in kinetics ...
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bioRxiv - Microbiology 2024Quote: ... coli protein synthesis system (New England Biolabs) was used for in vitro protein expression ...
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bioRxiv - Microbiology 2024Quote: Final PCRs on cDNAs were performed in a 100 µL scale using 2.0 µL precipitated cDNA in the presence of 1 x High-Fidelity PCR MasterMix (NEB), 2 µM forward primer with native barcode and 2 µM reverse primer with native barcode (e.g BC1 fwd and rev ...