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Citations for New England Biolabs :
51 - 100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... PCR-amplified ER-mRFP was cloned into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with BamHI-HF (New England Biolabs, R0136) and AgeI-HF (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR (qPCR) was performed using LUNA Universal qPCR Master Mix (New England Biolabs) on the 7500 real-time PCR machine (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, New England Biolabs) according to manufacturer’s instructions in combination with a poly(A ...
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bioRxiv - Cancer Biology 2024Quote: ... and phosphorylated with T4 polynucleotide kinase (NEB). First ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA (>300 ng) was reverse transcribed using the Lunascript RT Supermix Kit (NEB, E3010).
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bioRxiv - Cancer Biology 2024Quote: ... using the Luna Universal qPCR Master Mix (NEB, M3003), and included initial enzyme activation for 3 minutes at 95 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... and barcoded libraries were made using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Libraries were pooled and single-end sequenced (1×75 bp ...
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bioRxiv - Cancer Biology 2024Quote: ... Poly(A) RNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and barcoded libraries were made using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Cancer Biology 2024Quote: ... pA-Tn5 transposome was incubated with 150ng HMW Lambda DNA (NEB) in digestion buffer (5XTAPS-MgCl2-PEG8000 ...
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bioRxiv - Cancer Biology 2024Quote: ... prior to incubation with 1% SDS and proteinase K (20mg/mL, NEB) to inactivate and degrade pA-Tn5 ...
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bioRxiv - Cancer Biology 2024Quote: ... For the minigene constructs the intron and parts/complete adjacent upstream and downstream exons were PCR-amplified from K562 genomic DNA using Phusion Hot Start Flex polymerase (New England Biolabs) and cloned by the Hot Fusion (61 ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Placenta (M0307, New England Biolabs) and 25 U of M-MuLV Reverse Transcriptase with corresponding buffer (M0253 ...
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bioRxiv - Cancer Biology 2024Quote: ... Oligonucleotides for site-directed mutagenesis were designed using the NEBaseChanger version 1.3.3 (New England Biolabs) and are listed in Supplementary Table S4 ...
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bioRxiv - Cancer Biology 2024Quote: ... Mutations and insertions were introduced by site directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs). Oligonucleotides for site-directed mutagenesis were designed using the NEBaseChanger version 1.3.3 (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... and AgeI-HF (New England Biolabs, R3552) at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR-amplified HMGCR was cloned into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with BamHI (New England Biolabs, R0136) and XhoI (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by ligation with T4 DNA ligase (New England Biolabs, M0202) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... or pMAL-c2x (New England Biolabs), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... or Amylose Resin High Flow (New England Biolabs) respectively according to the manufacturers’ instructions.
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bioRxiv - Cancer Biology 2024Quote: ... After dephosphorylation with T4 Polynucleotide Kinase (NEB, #M0201S) the adapter Linker-1 (5’ rAppCTGTAGGCACCATCAAT/3ddC/ 3’ ...
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bioRxiv - Developmental Biology 2024Quote: ... pXPR_050 plasmids were digested using the Bsmb1 restriction enzyme (NEB; R0580). The synthesized double-strand oligo pools and digested pXPR_050 plasmids were ligated using T7 ligase (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... Purified RNAs were used for Illumina RNA-seq library preparation with NEBNext Ultra II Directional RNA Library Prep Kit (NEB; E7765), and a minimum of 20 million raw reads were obtained ...
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bioRxiv - Developmental Biology 2024Quote: ... both CRISPRi plasmid pools and genomic DNAs were PCR amplified with Miseq_Adaptor primers (Table S5) using Q5 High-Fidelity DNA Polymerase (NEB; M0491L). PCR samples were run on a 2% agarose gel and purified using a Gel Extraction Kit (Qiagen ...
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bioRxiv - Developmental Biology 2024Quote: ... Eluted DNA was amplified using NEBNext Ultra II PCR Master Mix (NEB) and purified using AMPure XP beads ...
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bioRxiv - Developmental Biology 2024Quote: ... The repair template was made by NEB assembly of upstream flanking sequence and degron from the repair template of degron::gfp and a synthetic gene fragment from IDT that contains a 3xFLAG tag and the downstream flanking sequence ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was amplified by PCR using the Q5 High-Fidelity DNA polymerase kit (New England BioLabs) following the manufacturer’s recommendations and with primers designed around the mutations (supplementary Table S9) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR reactions contained 2 μl cDNA in 50 μl PCR reaction with Phusion Hot Start Flex (New England Biolabs). Reaction conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using NEB UltraII RNA library Prep kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 7 ng of RNA per sample using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, E6420). Libraries were pooled and sequenced using a P3-100 kit from Illumina in a NextSeq2000 sequencer following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... RNA was quantified by Nanodrop and 1 µg of RNA per sample was treated for ribosomal RNA depletion using the NEBNext rRNA Depletion Kit (NEB, E6350) and then immediately followed by Library prep using NEBNext Ultra II kit (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 2 ng of RNA per sample using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, E6420). Libraries were pooled and sequenced using a P3-100 kit from Illumina in a NextSeq2000 sequencer following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... and then immediately followed by Library prep using NEBNext Ultra II kit (NEB, E7770). Libraries were pooled and sequenced using a H75 kit from Illumina in a NextSeq500 sequencer following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were quantified using the NEBNext Library Quant Kit (NEB, E7630S), then pooled at same molarity and paired-end sequenced with a P2-100 kit from Illumina in a NextSeq2000 sequencer following manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... Each 50 µL PCR2 reaction contains 25 µL of 2xQ5 Master Mix (New England Biolabs, M0494L), 5 µL of each index primer (N7xx or S5xx) ...
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bioRxiv - Cell Biology 2024Quote: ... Each 100 µL PCR1 reaction contains 50 µL of 2xQ5 Master Mix (New England Biolabs, M0494L), 0.2 µL of MgCl2 (stock concentration at 1M) ...
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bioRxiv - Molecular Biology 2024Quote: ... and further cleaning (to get rid of DNA contamination) with Monarch® Total RNA Miniprep Kit (NEB, T2010S) by following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by dA-tailing (NEB – E6053L) as per the manufacturers’ reaction conditions and instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The sonicated DNA was end-repaired by the End Repair Module (NEB – E6050L) and cleaned up with 2.2x AmpureXP beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... Illumina compatible adapters (IDT – xGEN UDI-UMI adapters) were ligated by using T4 ligase (NEB – M0202S) overnight at 16ºC ...
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bioRxiv - Molecular Biology 2024Quote: ... 2µL/sample Proteinase K(20ug/uL)(NEB,P8107S) and 1µL/sample Turbo DNase in 1X Turbo DNase Buffer (100µL/sample)(Thermo fisher ...
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bioRxiv - Microbiology 2024Quote: ... MssI and XhoI restriction enzymes and a Quick-Load® 1 kb Extend DNA Ladder (NEB). Plasmid curing was confirmed by loss of pESI related antibiotic resistance ...
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bioRxiv - Cancer Biology 2024Quote: ... and/or RNase H (New England Biolabs, M0297S) and incubated with rocking for 1 hr ...
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bioRxiv - Cancer Biology 2024Quote: ... ShortCut RNase III (New England Biolabs, M0245S), and/or RNase H (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... Then the PCR amplicon of Cdk1 CpG was digested by KpnI and MfeI (New England Biolabs) to remove the overhangs ...
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bioRxiv - Cell Biology 2024Quote: ... orthovanadate (200 nM; New England Biolabs, P0758S) and β-glycerophosphate (25 mM ...
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bioRxiv - Molecular Biology 2024Quote: ... were mixed with an equal volume of RNA loading dye (New England Biolabs), respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... adding nuclease-free water to a final volume of 1 L) with 1:1000 (vol/vol) proteinase K (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... the total RNA of each sample (50 μg) was treated with 1 μL of polynucleotide kinase (New England Biolabs) in 50 μL of total solution at 37℃ for 6 h ...
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bioRxiv - Molecular Biology 2024Quote: ... and XbaI (NEB #R0145) following the manufacturer’s instructions overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA fragments were combined with the digested pLenti plasmid in a Gibson Assembly reaction mix (NEB #M5510AA) at a molar ratio of 1:3:3 [vector:TurboID:UL26] for 1 hour at 50C ...