Labshake search
Citations for New England Biolabs :
2601 - 2650 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were ligated to pUCm-T vector (B522211, Sangon) with Blunt/TA Ligase Master Mix (M0367S, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... the primary probes were amplified using Phusion® High-Fidelity PCR Master Mix with HF Buffer (M0531S, NEB) by monitoring the amplification on a qPCR machine (CFX Connect ...
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bioRxiv - Genetics 2023Quote: All plasmids were generated using Gibson Assembly strategies67 using NEBuilder® HiFi DNA Assembly Master Mix (NEB #E2621) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... The T7 promoter and mtdTomato CDS were amplified from pmrPTRE-AAV using PTRE_floxed_F/R and Phusion High-Fidelity PCR Master Mix (NEB). NotI and SalI sites added by the primers were used to subclone this amplicon into pRM1506_TMM432 ...
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bioRxiv - Developmental Biology 2022Quote: ... Patient-derived variants were created by site-directed mutagenesis using Q5 High Fidelity Master Mix (NEB Catalog #M0492L), T4 polynucleotide kinase (NEB Catalog# M0201L ...
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bioRxiv - Cell Biology 2023Quote: ... A second PCR was performed using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs; #M0543) and Index 1 (i7 ...
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bioRxiv - Molecular Biology 2023Quote: ... while wheat AGPase LS was cloned into pUC-SYCE with the help of Gibson assembly master mix (NEB), using BamHI as restriction enzyme (Sharma et al. ...
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bioRxiv - Genomics 2023Quote: ... the oligo pool for each library was amplified with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541L) and the following primers ...
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bioRxiv - Biochemistry 2023Quote: ... Purified PCR products were ligated using the HiFi DNA Assembly master mix (New England Biolabs, catalog no. E2621S) into a Blasticidin-resistance encoding lentiviral vector (Addgene ...
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bioRxiv - Molecular Biology 2023Quote: ... Fragments were assembled using the NEBuilder® 2 X HiFi DNA assembly master mix (NEB, Cat. No. #E2621). The resulting plasmid will be referred to as pBd-phoD-HA-BSD.
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bioRxiv - Molecular Biology 2023Quote: ... Fragments were assembled using the NEBuilder® 2 X HiFi DNA assembly master mix (NEB, Cat. No. #E2621). The homology and guide RNA were inserted as described for pBdEF-Cas9-BSD-phodR ...
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bioRxiv - Genetics 2023Quote: ... Pooled DNA libraries were amplified in 50 µl reactions with 1x NEBNext High Fidelity PCR Master Mix (NEB) and 12.5 pmol PCR primers containing complementary sequences for adapter-ligated DNA ...
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bioRxiv - Synthetic Biology 2023Quote: ... The construct was built by Gibson Assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621). After assembling ...
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bioRxiv - Genomics 2023Quote: ... and were ligated at room temperature for 20 minutes using the Blunt/TA Ligase Master Mix (NEB M0367S). Transformation ...
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bioRxiv - Plant Biology 2023Quote: ... a new acceptor (pBy_01) was built using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, NEB #E2621). Four fragments were amplified by PCR using the Q5 High-Fidelity DNA Polymerase (NEB #M0492 ...
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bioRxiv - Plant Biology 2023Quote: ... a new acceptor (pBy_01) was built using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, NEB #E2621). Four fragments were amplified by PCR using the Q5 High-Fidelity DNA Polymerase (NEB #M0492 ...
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bioRxiv - Genomics 2023Quote: ... DNA was then pre-amplified 5 cycles with NEBNext Ultra II Q5 master mix (New England Biolabs, M0544) with a cycling protocol of 72°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were undertaken using sporocarp extractions using the PHusion High-Fidelity PCR Master Mix (New England Biolabs). Libraries were generated using the TruSeq DNA PCR-Free Prep Kit (Illumina ...
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bioRxiv - Genetics 2023Quote: ... Plasmids were constructed by Gibson Assembly using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs Cat. #E2621) and transformed into NEB 10-beta electrocompetent E.coli (New England BioLabs Cat ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmids were constructed in the present using NEB-HiFi DNA assembly master mix from NEB (Beverly, MA).
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bioRxiv - Synthetic Biology 2023Quote: The two plasmids pEKEx2_low_mCherry and pEKEx2_high_mCherry were assembled via Gibson cloning using the Gibson Assembly® Master Mix (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... muGFP and homology arms were PCR-amplified using Q5 High-Fidelity 2× Master Mix (New England BioLabs, M0492L). Primers for PCR were designed using the NEBuilder Assembly Tool ...
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bioRxiv - Microbiology 2023Quote: ... Purified PCR products were incubated together in a thermocycler with 1X Hi-Fi DNA Assembly master mix (NEB) at 50°C for one hour ...
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bioRxiv - Biochemistry 2024Quote: Plasmid constructs were cloned by Gibson assembly(51) using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Codon-optimised gene fragments for wild-type MxEnc and the triple mutants 3×His-MxEnc ...
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bioRxiv - Molecular Biology 2023Quote: ... were assembled into the Barcoded_AttB_EGFP-Link-PTEN-IRES-mCherry_Bgl2_160407 linearized vector by a Gibson reaction using the Gibson assembly master mix (New England Biolabs) by mixing the oligos with the vector in a 4:1 molar ratio ...
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bioRxiv - Microbiology 2023Quote: Plasmid mAID-5xTy/DHFR/OsTir1_N was constructed by Gibson assembly (NEBuilder® HiFi DNA Assembly Master Mix; NEB) of the following fragments ...
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bioRxiv - Biophysics 2023Quote: ... The DNA library was then prepared for Illumina NGS sequencing by PCR using Phusion HF master mix (NEB) and custom indexed primers for the PTM library 56 ...
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bioRxiv - Cell Biology 2023Quote: ChIP libraries were prepared using NEBNext ChIP-seq DNA Sample Prep Master Mix Set for Illumina (NEB E6240) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
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bioRxiv - Bioengineering 2023Quote: ... and NEBuilder® HiFi DNA Assembly Master Mix were purchased from New England Biolabs (NEB, Ipswich, MA, USA). Oligonucleotides for genetic manipulations were synthesized by Integrated DNA Technologies (IDT ...
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bioRxiv - Developmental Biology 2024Quote: ... were added to the amplicons using NEBNext® Ultra™ II Q5® Master Mix (NEB, cat. M0544S) for 7 cycles ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and all fragment ligation reactions were performed using NEBuilder HiFi DNA Assembly Master Mix (#M5520AA, New England Biolabs).
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bioRxiv - Cell Biology 2024Quote: ... Gibson Assembly of the three generated fragments was performed using NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621). The resulting assembly mixture was transformed into chemically competent Escherichia coli (C2987H ...
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bioRxiv - Genomics 2024Quote: ... 2 μL of 10% Tween-20 and 5 μL NEBNext High-fidelity 2× PCR Master Mix (NEB, M0541) was added to each well sequentially ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was performed on beads using NEBNext ChIP-Sequencing Library Prep Master Mix Set for Illumina (NEB). The manufacturer’s instructions were followed ...
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bioRxiv - Biochemistry 2024Quote: All truncations were made by using NEBuilder® HiFi DNA assembly master mix (M5520AVIAL, New England Biolabs, NEB) or using Q5 mutagenesis (E0555L ...
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bioRxiv - Biochemistry 2024Quote: All truncations were made by using NEBuilder® HiFi DNA assembly master mix (M5520AVIAL, New England Biolabs, NEB) or using Q5 mutagenesis (E0555L ...
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bioRxiv - Cancer Biology 2024Quote: ... and the adaptor ligated DNA was amplified using NEBNext Q5 Hot Start Hifi PCR Master Mix (NEB, M0543). The libraries were sequenced at a rate of 5 million reads per sample ...
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bioRxiv - Microbiology 2024Quote: ... and hrp2 terminator were seamlessly ligated into pSKA-001 vector using NEBuilder HiFi DNA Assembly Master Mix (NEB) and the intermediate fragment was cloned into pYCs-PbU6-hDHFR/yFCU-NS-p230p vector ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Transposed DNA was originally amplified and barcoded in a PCR reaction using NEBnext PCR master mix (NEB, M0544) and 1.25 μM forward and reverse primers originally described in 99 ...
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bioRxiv - Synthetic Biology 2024Quote: ... extracted PCR product with 2 μl of NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA expression was determined using SYBR green Luna Universal One-step RT-PCR kit (New England Biolabs, Cat #E3005L) with a BioRad CFX96 thermocycler ...
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bioRxiv - Pathology 2023Quote: ... Each 10 µL reaction contained Luna Probe One-Step RT-qPCR 4X Mix (New England Biolabs, Ipswich, MA, USA), primers (10 µM) ...
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bioRxiv - Genetics 2020Quote: ... and qPCR performed (NEB Luna qPCR MasterMix) on ald and rp49 (aldF2 ...
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bioRxiv - Microbiology 2020Quote: ... purified and ligated for 2 h at room temperature using the Instant Sticky-end Ligase Master Mix (NEB, Australia) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Tagmented library was amplified with P5_BRB and BRB_Idx7N5 primers (5 μL, Supplementary Table 14) using NEBNext UltraTM II Q5 Master Mix (NEB, M0544L) which was incubated at 98 °C for 30 sec before adding DNA with the following conditions ...
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bioRxiv - Genetics 2021Quote: ... The construct was assembled following the protocol described by NEBuilder® HiFi DNA Assembly Master Mix (NEB Catalog #E2621S) and confirmed by Sanger sequencing ...
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bioRxiv - Genetics 2021Quote: ... All other plasmids were assembled by Gibson cloning of relevant PCR products and/or custom designed gene blocks (gBlocks Gene Fragments, Integrated DNA Technologies) according to manufacturer’s protocol (Gibson Assembly Master Mix, NEB). The template plasmids for the codon-optimized SARS-CoV-2 S protein sequences were generously shared with us by Professor Robin Shattock from the Imperial College London (S-Imperial) ...
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bioRxiv - Microbiology 2020Quote: ... These gBlocks fragments and a NdeI-HindIII-digested pET21b backbone were assembled using a 2× Gibson master mix (NEB). Two and a half μL of each fragment at equimolar concentration was added to 5 μL 2× Gibson master mix (NEB) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... with 10–14 cycles of enrichment for each sample and using Phusion High-Fidelity Master Mix (NEB catalog #M0531). Samples were submitted for 101 bp paired-end sequencing on an Illumina HiSeq2000 at BGI-Hong Kong ...
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bioRxiv - Molecular Biology 2022Quote: ... The CXCL8 promoter was amplified by PCR with Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB) according to the manufacturer’s instruction with the following primers: