Labshake search
Citations for New England Biolabs :
2501 - 2550 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... PCR amplification was performed with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR products were digested with DpnI and assembled with Gibson assembly master mix (New England Biolabs) according to the manufacturer instructions ...
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bioRxiv - Genetics 2023Quote: ... Inserts were cloned into XbaI-and NotI-digested AAV backbones using HiFi DNA Assembly Master Mix (NEB). All PCRs were performed using Q5 High-Fidelity DNA Polymerase (NEB) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gibson Assemblies were performed using NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs Inc., U.S.A) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... PCR master mix (64 µL of H2O, 100 µL of Q5 high-fidelity DNA polymerase (NEB #M0544S), 8 µL of i5 primer ...
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bioRxiv - Microbiology 2023Quote: ... in a thermal cycler with a 2 × Q5 PCR master mix (New England Biolabs, Ipswich, MA, USA). The following program was used ...
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bioRxiv - Molecular Biology 2023Quote: Target sites of library-diverse were amplified by NEBNext Ultra II Q5 Master Mix (NEB, 26 cycles), and target sites from the TRIP library were amplified by GoTaq G2 Hot Start Green Master Mix (Promega ...
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bioRxiv - Bioengineering 2023Quote: ... PCR was performed using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs®) or RedTaq DNA Polymerase 2X Master Mix (VWR Life Science ...
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bioRxiv - Developmental Biology 2023Quote: ... The products were cloned into pRA638 using the NEBuilder HiFi DNA Assembly Master Mix (NEB, Ipswich, MA). All primers are listed in Supplemental Material (File S1 ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic PCR was carried out using NEBNext® Ultra™ II Q5® Master Mix (NEB, M0544S) to amply Mir96 loci ...
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bioRxiv - Immunology 2023Quote: ... 17 PCR cycles were performed using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532) with primers and template oligo each at a concentration of 10uM ...
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bioRxiv - Microbiology 2024Quote: ... inserted in plasmid vector pUC19 with the Gibson Assembly Master Mix kit (New England BioLabs, NEB # E2611S). Mutagenesis was carried out with the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Synthetic Biology 2024Quote: ... the plasmid is assembled with Gibson assembly protocol using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621S). Assembled DNA was transformed into competent cells ...
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Pre-implantation genome-wide methylation enables environmental adaptation in a social meso-carnivorebioRxiv - Genomics 2024Quote: ... These sub-libraries were barcoded and enriched using NEBNext Q5U Master Mix (New England Biolabs, United Kingdom). Sub-libraries were purified using 1.8× AMPureXP and pooled to equimolarity for the final library at ∼20 nM ...
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bioRxiv - Biophysics 2024Quote: ... A 673 bp digoxigenin-labeled DNA handle was amplified in Q5U PCR master mix (NEB, Cat# M0597L) with a forward primer containing a deoxyuridine and a reverse primer with a 5′ digoxigenin tag ...
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bioRxiv - Cell Biology 2024Quote: ... All sequences were inserted into L4440 using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) and transformed into DH5α bacteria.
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bioRxiv - Microbiology 2024Quote: ... amplified plasmid and insert PCR products were assembled using NEBuilder HiFi DNA Master Mix (New England Biolabs) following manufacturer’s protocols and transformed into Escherchia coli strain S-17 by electroporation for maintenance and replication ...
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bioRxiv - Microbiology 2024Quote: ... inserted in plasmid vector pUC19 with the Gibson Assembly Master Mix kit (New England BioLabs, NEB # E2611S). Mutagenesis was carried out with the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Cole) containing an N-terminal 6×His-tag using NEBuilder HiFi DNA Assembly Master Mix (NEB E2621L). The LSD1 constructs were expressed in BL21-CodonPlus (DE3)-RIPL competent E ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were generated using either standard restriction-ligation or assembled using the Gibson Assembly Master Mix (NEB) or In Vivo Assembly ...
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bioRxiv - Microbiology 2019Quote: ... or a homemade qPCR mix as described here: each 20 uL reaction contained 1X Thermopol Reaction Buffer (NEB), 125uM dNTPs ...
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bioRxiv - Cell Biology 2022Quote: ... were amplified from the corresponding pCMV6-AN-mGFP-PRR14 full-length vectors using Q5 Hot-start High-Fidelity Polymerase 2x Mix (New England Biolabs, cat# M0494S) and the following primers containing EcoRI and AgeI restriction sites (Forward (EcoRI) ...
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bioRxiv - Cell Biology 2022Quote: ... were amplified from the corresponding pCMV6-AN-mGFP-PRR14 full-length vectors using Q5 Hot-start High-Fidelity Polymerase 2x Mix (New England Biolabs, cat# M0494S) and the following primers containing HindIII and RsrII restriction sites (Forward (HindIII) ...
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bioRxiv - Molecular Biology 2021Quote: ... Phusion HF 2X (NEB, M0531L) 25 μl ...
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bioRxiv - Neuroscience 2021Quote: ... 2X Quick Ligase Buffer (NEB), ddH2O ...
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bioRxiv - Genetics 2021Quote: ... or LongAmp 2X (NEB, M0287S), according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... 12.5µL OneTaq 2X MasterMix (NEB) and 5µL nuclease-free water ...
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bioRxiv - Plant Biology 2020Quote: The mCherry plasmid FC1011 plasmid was constructed by NEBuilder HiFi DNA assembly Master Mix (New England Biolabs, MA) using pKA1 (CDKB plasmid ...
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bioRxiv - Synthetic Biology 2021Quote: ... The tagged pore variant plasmids were constructed by Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (NEB), where the variant encapsulin genes were individually amplified by PCR from the untagged pore variant plasmids ...
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bioRxiv - Cancer Biology 2021Quote: ... The regular PCR was performed using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs). The PCR products that targeted exon18-10 and exon9-10 were then combined for each sample and run on 2% agarose gel ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR reactions (total 30 μL) included 15 μL PhusionR High-Fidelity PCR Master Mix (New England Biolabs), 0.2 mM primers ...
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bioRxiv - Cell Biology 2019Quote: ... All constructs were generated using the NEBuilder® HiFi DNA Assembly Master Mix system (New England BioLabs, E2621) and sequenced to validate correct cDNA insertion ...
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bioRxiv - Molecular Biology 2021Quote: ... 6×50 µl PCR reactions were set up using NEBNext Ultra II Q5 master mix (New England Biolabs) with 5 nM oligos as template ...
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bioRxiv - Molecular Biology 2019Quote: ... or prepared for sequencing using the NEBNext DNA Library Prep Master Mix Set for Illumina (New England BioLabs). ChIP-seq data are available on the NCBI GEO database (GSE128317).
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bioRxiv - Molecular Biology 2019Quote: ... and a DNA fragment containing GFP were inserted into pCAGEN by NEBuilder HiFi DNA Assembly Master Mix (NEB).
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bioRxiv - Microbiology 2022Quote: ... The two fragments were ligated using the NEBuilder HiFi DNA Assembly master mix (New England BioLabs, Ipswich, MA) yielding the pHT009-cfr plasmid (VHp439 ...
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bioRxiv - Genomics 2020Quote: ... instead of SuperScript IV (Thermo) and half volume native barcoding reactions using Blunt/TA Ligase Master Mix (NEB) instead of NEBNext Ultra II Ligation Module (NEB).
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bioRxiv - Microbiology 2019Quote: ... genomic DNA libraries were prepared using NEBnext DNA Library Prep Master Mix Set for Illumina (New England Biolabs) following the instructions for 400 bp insert libraries ...
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bioRxiv - Cancer Biology 2019Quote: MIC ligands and orthogonal variants were cloned by ligation-independent assembly (HiFi DNA Assembly Master Mix, NEB #E2621) as fusions to the C-terminus of either the kappa light-chain or the heavy-chain of human IgG1 antibodies via either an APTSSSGGGGS or GGGS linker ...
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bioRxiv - Genetics 2020Quote: ... The four fragments were assembled using the NEB HiFi Assembly Master Mix according to the manufacturer’s recommendations (NEB), transformed into chemically competent DH5α (Invitrogen) ...
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bioRxiv - Immunology 2019Quote: ... and second strand synthesis was carried out by resuspending beads in a master mix containing Klenow Fragment (NEB), dNTPs ...
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bioRxiv - Genomics 2020Quote: ... Strand extension reaction was performed by directly adding 0.32 U/μl Bst 3.0 DNA Polymerase and 1X NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB) to tagmentation products and incubating at 72°C for 15 min ...
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bioRxiv - Systems Biology 2020Quote: ... and second strand synthesis was carried out by resuspending beads in a master mix containing Klenow Fragment (NEB), dNTPs ...
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bioRxiv - Bioengineering 2020Quote: ... DNA assembly was mainly done by using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, US) unless specified otherwise ...
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bioRxiv - Biochemistry 2020Quote: ... (1.5 μM x 2.5 μL in H2O) followed by addition of 4.5 μL NEBNext Ultra II Pol master mix (NEB). The reaction mixture was denatured at 98 °C for 1 min ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplification was performed in 1X Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB M0531) and PCR cycling at 98°C for 30s ...
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bioRxiv - Biophysics 2021Quote: ... Other constructs were cloned by PCR and DNA assembly (NEBuilder HiFi DNA Assembly Master Mix; New England Biolabs). The RGG domain used here is the N-terminal IDR (residues 1-168 ...
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bioRxiv - Plant Biology 2021Quote: ... transposed DNA was PCR amplified with 12 cycles using Next High-Fidelity 2×PCR Master Mix (NEB, M0541) with Nextera DNA CD Index primers ...
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bioRxiv - Systems Biology 2021Quote: ... The primers were designed to support HiFi DNA assembly (NEBuilder HiFi DNA Assembly Master Mix, New England BioLabs): dxs_thermus_F (5’-AAACCATGGAGGTGCGCGATATGATCTTGGACAAGGTGAAC-3’ ...
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bioRxiv - Systems Biology 2020Quote: ... DNA assembly reactions using 0.4 pmol insert and 0.1 pmol vector per 20 μL HiFi Master Mix (New England Biolabs) were incubated at 50°C for 1 h ...