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Citations for New England Biolabs :
2401 - 2450 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Transposed DNA was amplified in a solution containing 1X NEBNextHigh Fidelity PCR Master Mix (NEB #M0541L), 0.5 μM of Ad1.1 universal primer ...
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bioRxiv - Molecular Biology 2024Quote: ... The digested backbone and amplified genes were ligated using NEBuilder HiFi DNA Assembly Master Mix (NEB) and transformed into chemically competent E ...
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bioRxiv - Cell Biology 2024Quote: ... Eluted DNA was enriched by PCR using Phusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was conducted with NEBNext Library Prep Master Mix Set for Illumina (New England Biolabs) with NEBNext Multiplex Oligos for Illumina (New England Biolabs) ...
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bioRxiv - Plant Biology 2024Quote: ... Purified PCR fragments were assembled using Gibson Assembly® Master Mix (New England Biolabs, MA, USA) following the manufacturer’s procedures.
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bioRxiv - Bioengineering 2024Quote: ... Gibson assembly reactions were carried out with the NEBuilder Hifi DNA Assembly Master Mix (E2621, NEB) following supplier instructions ...
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bioRxiv - Genetics 2024Quote: ... and assemblies were performed using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, E2621). All regions amplified by PCR were confirmed by DNA sequencing at Genewiz from Azenta Life Sciences (South Plainfield ...
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bioRxiv - Microbiology 2024Quote: ... Gibson assembly were done using the NEB Builder HiFi DNA Assembly Master Mix (New England Biolabs) and transformed into E ...
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bioRxiv - Molecular Biology 2024Quote: ... Fragments were then ligated with Gibson assembly (NEBuilder® HiFi DNA Assembly Master Mix #E2621L, NEB).
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bioRxiv - Molecular Biology 2022Quote: ... Real-time PCR was performed using SYBR green gene expression assays (New England Biolabs, catalog no. M3003S) on a QuantStudio 6 instrument (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Q5 2X MM (NEB) was used following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 2X loading dye (NEB) was added the RNA and the sample was denatured at 70°C for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was amplified with a first set of oligos in a PCR reaction on a qPCR machine to detect amplification in real time to capture linear amplification (0.75 μl of 20 mM P3tall and P6tall oligo mix, 15 μl of 2x NEB Next (M0541L), 14 μl cDNA ...
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bioRxiv - Biochemistry 2023Quote: ... the δ’ fragment was inserted into the pET28PP backbone via Gibson assembly (2x NEBuilder Hifi DNA Assembly Mix, New England Biolabs). Expression plasmids were transformed into Mix & Go competent DH5a E ...
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bioRxiv - Genomics 2021Quote: ... Synthesis of the second cDNA strands were carried out with LongAmp Taq Master Mix (New England Biolabs). The double-stranded cDNAs were repaired (NEBNext End repair /dA-tailing Module ...
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bioRxiv - Cell Biology 2020Quote: All sequences were inserted into L4440 using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) and transformed into DH5a bacteria ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.2 μM primers (primer sequences are presented in Table S1) and 2 × PCR Master mix (M0482S, NEB), with the following cycling parameters ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We then performed PCR reactions at 12 cycles using Phusion High-Fidelity PCR Master Mix (NEB Biolabs) and primers that bind to common regions in the adaptors ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We then performed PCR reactions at 12 cycles using Phusion High-Fidelity PCR Master Mix (NEB Biolabs) and primers that bind to common regions in the adaptors ...
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bioRxiv - Microbiology 2021Quote: ... were combined in a Gibson reaction using NEBuilder HiFi DNA Assembly Master mix (New England Biolabs, E2621) using manufacturer’s protocols save for a 1 hour incubation step rather than 15 minutes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Assembly of the construct was done using the NEBuilder® HiFi DNA Assembly master mix (NEB, E2621)
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bioRxiv - Developmental Biology 2021Quote: ... Tn5 treated DNA was amplified with Nextera Library primers using NEBNext library amplification master mix (NEB M0541) and sequenced on an Illumina NextSeq ...
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bioRxiv - Genomics 2022Quote: ... digested empty vector A or P by gibson assembly NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621) in an 80 μL reaction ...
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bioRxiv - Genomics 2020Quote: ... and the NEBNext End repair/dA-tailing Module NEB Blunt/TA Ligase Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Barcode adapters were ligated to end-prepped samples using Blunt/TA Ligase Master Mix (New England Biolabs).
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bioRxiv - Genomics 2020Quote: ... Libraries for Illumina sequencing were prepared following the NEBNext DNA Library Prep Master Mix kit (NEB E6040). Amplified libraries were size selected using a 2% agarose gel to extract fragments between 200 and 600 bp.
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bioRxiv - Genomics 2019Quote: ... these three DNA fragments were assembled with NEBuilder® HiFi DNA Assembly Master Mix (Cat. E2621L, NEB). BamHI restriction site was introduced between the two homology arms during the PCR amplification ...
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bioRxiv - Molecular Biology 2019Quote: ... was introduced via PCR overlap extension using Gibson Assembly® Master Mix (New England Biolabs cat. #E2611), following the manufacturer’s instructions ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... The fragments were then joined using the NEB builder HiFi DNA Assembly Master Mix (New England Biolabs). The construct was made using pUC19 digested with BamHI as the vector backbone ...
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bioRxiv - Microbiology 2019Quote: ... PCR was performed on a plasmid template using the Q5 High-fidelity 2× Master Mix (NEB, M0492L), with primers at final concentration of 500nM ...
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bioRxiv - Cell Biology 2019Quote: ... 0.25μl of each primer (from 25μM stock) and 4.5μl of Luna Universal Master Mix (New England Biolabs). The q-RT-PCR reactions were run on the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... from our construct using primers 3xFLAG_F and 3xFLAG_R and cloned into petSUMO_NSP1_WT vector using Gibson Assembly® Master Mix (NEB). The resulting proteins were expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Genomics 2019Quote: ... The deaminated DNA were amplified with Q5 dU Bypass DNA polymerase Master Mix (NEB, Ipswich MA, USA) and the resulting libraries were analyzed and quantified with an Agilent Bioanalyzer 2100 DNA High-sensitivity chip ...
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bioRxiv - Genomics 2021Quote: ... The purified fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs Japan, Tokyo) at 50 °C (3 h) ...
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bioRxiv - Bioengineering 2019Quote: ... whereas colony PCRs were performed using 2xOneTaq Quick-Load Master Mix with Standard Buffer (New England Biolabs). Genomic DNA was extracted from overnight cultures using Yeast DNA Extraction Kit (Thermo Scientific) ...
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... in frame with the N-terminal His6-tag using the Gibson assembly master mix (New England Biolabs) for TaSS4-1B ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions were performed with the Q5 Hot Start High-Fidelity 2× Master Mix (New England Biolabs) with 100 ng of genomic DNA ...
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bioRxiv - Microbiology 2021Quote: ... and 20 ng of mRFP PCR products were incubated with Gibson master mix (New England Biolabs, #E2611S) at 50°C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... All PCR steps were carried out using the Phusion High-Fidelity PCR Master Mix (New England Biolabs). The PCR products were electrophoresed on a 2% agarose gel and stained with SYBR Safe DNA gel stain ...
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bioRxiv - Cell Biology 2021Quote: ... Annealed protospacer fragments were transferred to vectors using Instant Sticky-End Ligation Master Mix (New England Biolabs). Sec23B and G protospacers designed to tag these genes via HDR as well as Sec23A ...
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bioRxiv - Molecular Biology 2022Quote: ... 12.5 µl of Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB, Catalog no. M0531S) 0.8 µM of each primer (final concentration ...
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bioRxiv - Microbiology 2022Quote: ... The cloning procedure of gRNA was carried out using DNA Assembly Master mix (New England Biolabs, Inc). Wild type D10 P ...
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bioRxiv - Genetics 2022Quote: ... Phusion® High-Fidelity PCR Master Mix was used to prepare the PCR library (New England Biolabs). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... 45 nM pegRNA3-3AGTGA (Appendix Table S1) and 5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB) and incubated at 37°C for 1 h.
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bioRxiv - Neuroscience 2020Quote: ... PCR(20 total cycles) was then carried out using the Q5 Ultra II Q5 Master Mix(NEB) to amplify final libraries using universal Illumina primers ...
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bioRxiv - Microbiology 2021Quote: The pLentiCMV-TurboGFP::Luciferase fusion reporter gene was constructed using Gibson Assembly Master Mix (New England Biolabs) to exchange the insert in pLenti-CMV-Blast-EphA7-Strep (described elsewhere (57) ...
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bioRxiv - Genetics 2022Quote: ... These fragments were cloned with the pMTU-DO-G418-C5 backbone using Gibson Assembly Master Mix (NEB) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Library was prepared using NEBNext DNA Library Prep Master Mix Set for Illumina (NEB E6040, M0543L, E7335S) according to manufacture instruction ...
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bioRxiv - Synthetic Biology 2019Quote: ... both home-made mix36 and Gibson Assembly Master Mix were used (New England Biolabs, Ipswich, Massachusetts, USA). The complete list of constructed and used plasmids and primers can be found in Supplementary Tables S5 and S6 ...
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bioRxiv - Molecular Biology 2019Quote: ... ChIP-seq library construction was performed using the NEBNext ChIP-Seq Library Prep Master Mix Set (NEB). Libraries were sequenced on the Illumina HiSeq 2000/2500 platform ...