Labshake search
Citations for New England Biolabs :
2751 - 2800 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The PCR reaction was performed using NEBNext® High-Fidelity 2× PCR Master Mix (New England BioLabs, catalog #M0541) with a reaction volume of 10 ul including 5 ul 2× PCR Master Mix ...
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bioRxiv - Microbiology 2023Quote: ... Cloning of the expression vector was performed using NEBuilder HiFi DNA Assembly Master Mix (Gibson cloning) and was performed according to manufacturer’s guidelines (cat. n° E2621L, New England Biolabs). In addition ...
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bioRxiv - Genomics 2023Quote: ... we amplified pegRNA/ngRNA pair sequences from each sample using NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541L) and the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... Targeting constructs used as templates for homology-directed repair were Gibson-assembled using Gibson master-mix (New England Biolabs) and PCR-amplified homology arms corresponding to genomic sequence flanking the desired site of insertion ...
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bioRxiv - Molecular Biology 2023Quote: ... the product was purified using magnetic SPRI beads and amplified using 0.5 µM i5/i7 Illumina indexed primers in Q5 High-Fidelity Master Mix (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... Homology fragments were assembled using the NEBuilder® 2 X HiFi DNA assembly master mix (NEB, Cat. No. #E2621), while guide RNAs were inserted by ligation using T4 ligase (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... the parts were assembled with Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix from NEB, 1h at 50°C) with the linkers providing sequence overlaps ...
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bioRxiv - Microbiology 2023Quote: ... The purified products were ligated to obtain the rpoD-attB_KP35 fragment using the NEBuilder HiFi DNA assembly master mix (New England Biolabs). To obtain a high yield ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed and amplified using 1.25 μM Nextera index primers and NEBNext High-Fidelity 2xPCR Master Mix (New England BioLabs). A quantitative PCR was run to determine the optimal number of cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... These PCR amplicons were gel purified and then assembled using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs) and transformed into ChemiComp GT115 competent cells (InvivoGen) ...
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bioRxiv - Biochemistry 2023Quote: ... DNA oligonucleotides were amplified using Phusion® High-Fidelity PCR Master Mix with HF Buffer (M0531L, New England BioLabs). AmpliScribe™ T7-Flash™ Transcription Kit (ASF3507 ...
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bioRxiv - Genetics 2023Quote: ... Libraries for sequencing were amplified using unique dual index Illumina adapters and NEBNext Ultra II Q5 Master Mix (NEB). One purification step with Agencourt Ampure XP beads was performed before and after library amplification ...
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bioRxiv - Developmental Biology 2023Quote: ... A primer set annealing to the amplification sequences was used at a final concentration of 0.5 μM to amplify the pool of oligonucleotides using 12.5 μL 2 x NEBnext PCR master mix (New England BioLabs) and 3 μL of oligonucleotide pool (∼3 ng) ...
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bioRxiv - Genomics 2023Quote: ... All PCR reactions were performed with 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... PCR fragments and SrfI-digested vector were combined with NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs #E2621) in a 2:1 ratio of insert to vector ...
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bioRxiv - Biochemistry 2023Quote: ... and DDRGK1207-305-UFL127-200 (corresponding to DDRGK1-UFL1ΔN in the main text) (Figure 1b) were generated using Gibson assembly (Gibson assembly master mix, New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... 24–27 and were integrated into pENTR1A using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA) to obtain gateway entry clones (pENTR1A-LjbHLH32 and pENTR1A-LjbHLH50) ...
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bioRxiv - Immunology 2023Quote: ... Sequencing adapters were ligated to the end-repaired strands using Blunt/TA Ligase Master Mix (New England Biolabs, M0367L). The cDNA libraries were purified after each enzymatic reaction using the AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: ... Input genomic DNA was first amplified in a 10μL reaction for 30 cycles using NEBNext High-Fidelity 2×PCR Master Mix (NEB). Amplicons were purified using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Biophysics 2023Quote: ... and used in equimolar amounts in a Gibson assembly reaction with NEBuilder HiFi DNA Assembly Master mix (NEB # E2621S). The reaction was incubated at 50°C for 60 min and directly transformed into chemically competent NEB 10-beta E ...
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bioRxiv - Molecular Biology 2023Quote: ... Overlapping sequence was included to allow these amplicons to be combined with a 7.8kB NdeI/SalI fragment of the pCW57.1 lentiviral vector via Gibson assembly using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Products were confirmed with Sanger sequencing (Genewiz ...
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bioRxiv - Synthetic Biology 2024Quote: ... and designed for library variant expression upon induction by IPTG—using NEBuilder Hifi DNA Assembly Master Mix (NEB, E2621L). All libraries were then precipitated with isopropanol ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... 100 ng digested pMB1-10x and 2.4 ng amplified sgRNA library insert were assembled using Gibson Assembly Master Mix (NEB) in a 20 μL reaction for 30 min ...
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bioRxiv - Synthetic Biology 2024Quote: Gibson assemblies of each promoter library were executed using NEBuilder Hifi Assembly Master Mix (New England Biolabs, Ipswich, MA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... sgRNA libraries were recovered from gDNA via PCR amplification using NEBNext Ultra II Q5 Master Mix (New England BioLabs) according to manufacturer instructions and using custom primers (Extended Data Table 4) ...
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bioRxiv - Cancer Biology 2023Quote: ... The amplified crRNA library was then gel purified and assembled into BsmBI/Xhol-digested pLentiRNAGuide_001 using NEBuilder HiFi DNA Assembly master mix (NEB). The assembled plasmids were purified and concentrated by isopropanol precipitation ...
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bioRxiv - Plant Biology 2024Quote: ... The sequencing libraries were prepared by PCR amplification using the NEBNext PCR master mix (New England Biolabs, no. M0541S). The amplified libraries were purified with AMPure beads (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... and menA were amplified by PCR from the IR715 genome using Q5 High Fidelity Master Mix (New England Biolabs). PCR products were purified using the QIAEX II Gel Extraction Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... Single stranded oligo pools were ordered from IDT (oPools™ Oligo Pools) and built into the digested PCC_01 vector using NEBuilder HiFi DNA Assembly Master Mix (E2621L, New England Biolabs) according to the manufacturer’s instruction ...
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bioRxiv - Synthetic Biology 2024Quote: ... and mCherry coding sequences at an AGGT site via Gibson assembly using Hifi DNA Assembly Master Mix (NEB, M5520) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... the HIRA-WT and HIRA-W799A-D800A coding sequences.59 using NEBuilder® HiFi DNA Assembly Master Mix (NEB) and verified all constructs by Sanger sequencing ...
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bioRxiv - Microbiology 2024Quote: ... The RT RNA sample (5 μL) was amplified using the LongAmp Taq 2× Master Mix (NEB, Ipswich, MA, USA) with IVT Nanopore T7 Fw and IVT Nanopore T7 Rv primers (Supplementary Table 5) ...
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bioRxiv - Genomics 2024Quote: ... were carried out for a final volume of 12.5 µL containing 1x OneTaq HS Quick-Load Master Mix with Standard Buffer (NEB), 0.2 µM of each forward and reverse Intron-flanking primers (Supplementary Table 7) ...
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bioRxiv - Microbiology 2024Quote: ... Chromosomal integration of fluorescent tags was confirmed by colony PCR (touchdown protocol) using Q5 HF Master mix (NEB, USA). The primer pair Fwd_full series_Tn7 with Rev_Tn5/7_gt were used to confirm the integration of fluorescent tags ...
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bioRxiv - Bioengineering 2024Quote: ... The two obtained PCR fragments were assembled by NEBuilder® HiFi DNA Assembly Master Mix (E2621, New England BioLabs) into a pET28a backbone generated by PCR with primers 13 and 14 ...
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bioRxiv - Bioengineering 2024Quote: ... were mixed in molar ratio 1:1 and 0.37 pmol of total DNA was then treated by Gibson Assembly Master Mix (New England Biolabs) for 3 hours at 50°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and introduced into the pLIC expression vector55 by Gibson cloning (Gibson Assembly Master Mix, New England Biolabs, USA-MA). All other extant and ancestral CS sequences were obtained as gene fragments from Twist Bioscience (USA-CA ...
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bioRxiv - Immunology 2024Quote: ... Samples were then processed for library barcoding and amplification with Q5 High-Fidelity 2× Master Mix (cat. M0492S, NEB). Prepared libraries were sent for sequencing after quantification using Qubit and size distribution as determined using an Agilent 4200 TapeStation.
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bioRxiv - Synthetic Biology 2024Quote: ... Protein expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix, New England Biolabs, catalog no. E2611) into a pET-28c plasmid backbone and were designed to overexpress recombinant proteins as C-terminus His-tagged fusions ...
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bioRxiv - Molecular Biology 2024Quote: ... pBAD-His6-Ocr was obtained by cloning 0.3 gene from T7 phage into pBAD-HisB43 vector using Gibson Assembly Master Mix (NEB). Introduction of the overlapping BREX/AluI site to the pHERD30t vector was also achieved via Q5 site-directed mutagenesis kit (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... standard curve cDNAs were then serially diluted ten-fold from 10-1 to 10-8 and run through the SYBR Green assay (New England Biolabs) together with sample cDNAs ...
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bioRxiv - Cancer Biology 2021Quote: ... OneTaq 2x MasterMix (New England Biolabs), and 10μM each of F/R primers ...
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bioRxiv - Cancer Biology 2021Quote: ... OneTaq 2x MasterMix (New England Biolabs), and 10 μM each of F/R primers ...
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bioRxiv - Cancer Biology 2021Quote: ... OneTaq 2x MasterMix (New England Biolabs), and 10 μM each of F/R primers ...
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bioRxiv - Cancer Biology 2021Quote: ... OneTaq 2x MasterMix (New England Biolabs), and 10 μM each of F/R primers ...
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bioRxiv - Cancer Biology 2021Quote: ... OneTaq 2x MasterMix (New England Biolabs), and 10μM of each F/R pre-amplification primer to common flanking sequences (Forward ...
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bioRxiv - Cancer Biology 2021Quote: ... OneTaq 2x MasterMix (New England Biolabs), and 10 μM each of F/R primers ...
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bioRxiv - Immunology 2021Quote: ... 2X Q5 High Fidelity Mastermix (NEB), dNTP (10 mM each) ...
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bioRxiv - Molecular Biology 2022Quote: ... via Gibson assembly (NEB 2X HiFi) with a HindIII-digested pGL3-Basic and a gBlock (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... RT-qPCR was performed on a CFX Connect thermocycler with the Luna Universal or Probe one-step qPCR Master Mixes (New England Biolabs, Ispwich, MA). RNA (1-4 ng ...