Labshake search
Citations for New England Biolabs :
2701 - 2750 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2019Quote: ... library amplification was performed by setting up a PCR reaction (50 μl) with 1X Phusion HF master mix (NEB), 0.2 uM universal primer ...
-
bioRxiv - Genomics 2021Quote: DNA ends were marked with biotin-14-dATP by adding 60μL of biotin fill-in master mix (1X NEB 3.1 ...
-
bioRxiv - Genomics 2021Quote: ... Illumina libraries were then prepared using the NEBNext DNA Sample Prep Master Mix Set (New England Biolabs, MA, USA) and DNA fragments were PCR-amplified using Platinum Pfx DNA polymerase (Invitrogen ...
-
bioRxiv - Genetics 2021Quote: ... We added 1 µL 12.5% Triton-X to each well to quench the SDS and 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB). Samples were PCR-amplified (72 °C 5 min ...
-
bioRxiv - Genetics 2020Quote: ... The Rad21l1 PCR product was then cloned into pET28b using NEBuilder HiFi DNA Assembly Master Mix (NEB, Catalog #: E5520S). BL21 (DE3 ...
-
Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... of SPG15 was amplified from SPG15 cDNA by PCR and cloned in-frame into Xho1 and BamH1 cut pEGFP_C1 using Gibson Assembly Master Mix (E2611, New England BioLabs), and transferred into pLXINmod using EcoR1 and BamH1 and Rapid DNA Ligation Kit (11635379001 ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 μl of this lysate was directly used for PCR reactions with 2× Taq start master mix (M0496L, NEB), and the two NASP clone screening primers (see primer table) ...
-
bioRxiv - Microbiology 2021Quote: ... 22.5 µL of this was taken forward for native barcoding with the addition of 2.5 µL barcode and 25 µL Blunt/TA Ligase Master Mix (NEB) (final volume 50 µL) ...
-
bioRxiv - Genomics 2020Quote: ... followed by addition of 20 μl Ligation master mix (containing: 15 μl NEB Quick ligation buffer, 0.4 μl NEB Quick Ligase ...
-
bioRxiv - Genomics 2021Quote: ... DNA libraries were constructed using the NEBNext DNA Library Prep Master Mix Set for Illumina (NEB E6040, M0543L, E7335S). To elute DNA ...
-
bioRxiv - Microbiology 2021Quote: ... without codon optimization and was inserted into pcDNA 3.1 to g et pcDNA 3.1-SARS-CoV-2-Spike using NEBuilder® HiFi DNA Assembly Master Mix (NEB) a ccording to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... the linearized pDR111 was ligated with rapX and with spsX using Gibson Assembly® Master Mix (New England BioLabs), resulting in pDR111_rapX and pDR111_spsX ...
-
bioRxiv - Microbiology 2020Quote: ... regions of ~600 bp containing the sgRNA sequence were amplified using NEBNext Ultra II Q5 Master Mix (NEB #M0544S) in 25 amplification cycles ...
-
bioRxiv - Microbiology 2021Quote: ... End-prepared DNA was ligated with native barcode adapters NBD04 using Blunt/TA Ligase Master Mix (M0367S, NEB, USA). Following the barcode ligation reaction ...
-
bioRxiv - Molecular Biology 2021Quote: ... Each amplification template containing appropriate copy number of the gene was added into the master mix containing 1 μL of Bst 3.0 DNA polymerase (Cat. No. M0374L, NEB), 2.5 μL of 10 × isothermal amplification buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... the first step PCR reaction was performed in a 12.5 μl mixture containing 1X OneTaq Hot Start Master Mix (NEB), 0.4 μM of each DHPV-U 1538F and DHPV-U 1887R primer and 20 ng of DNA template ...
-
bioRxiv - Cell Biology 2021Quote: ... from E14 cells genomic DNA and cloned in pUC19 vector using Gibson Assembly Master Mix kit (New England Biolabs). The targeting construct was designed such that the guide RNA sequence used for the specific targeting was interrupted by the tag or contained silent mutations.
-
bioRxiv - Genomics 2021Quote: ... the Hi-C library was amplified for about 10 cycles of PCR with the Q5 master mix (NEB, M0492L), following the manufacturer’s instructions ...
-
bioRxiv - Genetics 2019Quote: ... EcoRV-digested amplicons encoding gRNA pairs were inserted into the vector by Gibson assembly (NEB Gibson Assembly Master Mix) according to manufacturer’s specifications ...
-
bioRxiv - Molecular Biology 2022Quote: ... were constructed from a pSB189L supF shuttle vector (11,12,15) using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, USA). Briefly ...
-
bioRxiv - Biochemistry 2022Quote: ... were assembled using 10 μl of 2× master mix at 50 °C for 1 h according to manufacturer’s instructions (NEB). 5α Competent Escherichia coli (30 μl ...
-
bioRxiv - Developmental Biology 2022Quote: ... All cDNA constructs were assembled in a pCDNA3.1+ vector by Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Sequences were confirmed with Genewiz sequencing service (South Plainfield ...
-
bioRxiv - Genetics 2022Quote: ... 1 µL of extracted genomic DNAs and 5 µL of 2× Hot Start High Fidelity Q5 Master Mix (NEB). PCR reactions were carried out under the following condition ...
-
bioRxiv - Microbiology 2022Quote: ... was generated by two fragment Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA). The plasmid pACYC184 (pACYCev ...
-
bioRxiv - Molecular Biology 2022Quote: ... the products were amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs) with 200 nM of Illumina multiplex primer and 200 nM of Illumina index barcode primers (98°C for 10 sec pre-denaturation followed by 12 cycles of 98°C for 5 sec ...
-
bioRxiv - Microbiology 2022Quote: ... and the three PCR products were joined using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs) according to manufacturer’s recommendations and transformed into NEB5α cells (New England Biolabs) ...
-
bioRxiv - Synthetic Biology 2022Quote: For all approaches DNA-Assembly was conducted using the NEBuilder DNA-Assembly Master Mix (New England Biolabs, Ipswich, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... All plasmids (Table S4) were constructed using the Gibson isothermal assembly method (NEBuilder HiFi DNA assembly master mix, NEB) and electrotransformed into E ...
-
bioRxiv - Zoology 2023Quote: ... We performed 5 PCR enrichment with 15 cycles (30 ng DNA input, NEB Phusion High-Fidelity PCR Master Mix) for each library to increase fragment diversity ...
-
bioRxiv - Systems Biology 2022Quote: ... PCRs for screens with all other libraries were performed using the NEBNext Ultra II Q5 Master Mix (NEB #M0544L) with 21 to 24 cycles ...
-
bioRxiv - Neuroscience 2022Quote: ... The last two PCR rounds were performed using NEBNext® Ultra™ II Q5® Master Mix (NEB #M0544). Samples were purified using AMPure XP beads (Beckman Coulter #A63880) ...
-
bioRxiv - Microbiology 2022Quote: ... Conventional PCR assays were carried out with Q5 Hot Start High-Fidelity 2× Master Mix (New England Biolabs, Australia). For some samples ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 0.18 pmol of the insert DNA fragment and 0.02 pmol of the vector DNA fragment were mixed with NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) to make a total volume of 10 µl ...
-
Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... 100 ng digested pMB1-10x and 2.4 ng amplified sgRNA library insert were assembled using Gibson Assembly Master Mix (NEB) in a 20 μL reaction for 30 min ...
-
bioRxiv - Synthetic Biology 2024Quote: Gibson assemblies of each promoter library were executed using NEBuilder Hifi Assembly Master Mix (New England Biolabs, Ipswich, MA) following the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2024Quote: ... The sequencing libraries were prepared by PCR amplification using the NEBNext PCR master mix (New England Biolabs, no. M0541S). The amplified libraries were purified with AMPure beads (Beckman Coulter ...
-
bioRxiv - Cancer Biology 2024Quote: ... Single stranded oligo pools were ordered from IDT (oPools™ Oligo Pools) and built into the digested PCC_01 vector using NEBuilder HiFi DNA Assembly Master Mix (E2621L, New England Biolabs) according to the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2024Quote: ... and menA were amplified by PCR from the IR715 genome using Q5 High Fidelity Master Mix (New England Biolabs). PCR products were purified using the QIAEX II Gel Extraction Kit (Qiagen) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and designed for library variant expression upon induction by IPTG—using NEBuilder Hifi DNA Assembly Master Mix (NEB, E2621L). All libraries were then precipitated with isopropanol ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.01 pmol (2:1 molar ratio of insert:backbone) library and 2.5 uL NEBuilder HiFi DNA Assembly Master Mix (NEB E2621) and incubated at 50 °C for 60 minutes ...
-
bioRxiv - Molecular Biology 2024Quote: ... The second strand synthesis was then performed by adding 100 μl Q5 Hot Start High Fidelity Master Mix (NEB), 10 μl RNase H (NEB) ...
-
bioRxiv - Neuroscience 2024Quote: ... Input genomic DNA was first amplified in a 10μL reaction for 30 cycles using NEBNext High-Fidelity 2×PCR Master Mix (NEB). Amplicons were purified using AMPure XP beads (Beckman Coulter ...
-
bioRxiv - Molecular Biology 2024Quote: All cloning was performed using Gibson cloning with the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). The PAGFP-TIRR plasmid was generated by amplifying a PAGFP fragment from a pPAGFP-C1 plasmid and a backbone containing TIRR using Q5® High-Fidelity DNA Polymerase and associated Q5® Reaction Buffer and GC enhancer (New England Biolabs) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and assembled with HindIII-digested pUC19mPheS plasmid using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs Inc.). The resulting plasmid was then transformed into E.coli DH10b strain for isolation via QIAprep Plasmid Mini-Prep Kit (Qiagen) ...
-
bioRxiv - Biophysics 2024Quote: ... pCAGEN_6×His-DNAJA2-HA was created from PCR of the ORF from pColdI_6×His-DNAJA2 and insertion into EcoRI-digested pCAGEN plasmids using NEBuilder HiFi DNA Assembly Master Mix (NEB). This plasmid was then used to produce pCAGEN_3xFLAG-DNAJA2 in a similar manner ...
-
bioRxiv - Cancer Biology 2023Quote: ... Addgene cat#52961) by PCR amplification using NEB Next High-Fidelity PCR Master Mix (New England Biolabs, cat#M0541S). The PCR product was digested with Esp3I (BsmBI ...
-
bioRxiv - Genetics 2024Quote: ... The PCR products were cloned into the pSpl3-Flu2-TK-del vector using Gibson Assembly Master Mix (NEB, USA).
-
bioRxiv - Genomics 2024Quote: ... using the barcodes provided and the NEB Blunt/TA Ligase Master Mix (New England Biolabs UK, Catalogue number: M0367L), with samples then being pooled together and cleaned as before ...
-
bioRxiv - Molecular Biology 2023Quote: The cloning procedures were performed as previously described (Düsterhöft et al., 2021): NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621L) was utilised to produce different plasmids with the desired inserts according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... the pooled oligo library was amplified using NEB Next High-Fidelity PCR Master Mix (New England Biolabs, cat#M0541S). The PCR product was digested with Esp3I (BsmBI ...