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Citations for New England Biolabs :
2651 - 2700 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 22.5 µl of cDNA was combined with 2.5 µl native barcode and 25 µl Blunt/TA Ligase Master Mix (New England Biolabs). Samples were incubated at room temperature for 10 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... and No Template Controls (NTC) for each primer pair using Luna Universal qCPR Master Mix (NEB, Ipswich, MA, #M3003) following the manufacturer’s protocol (1ul cDNA ...
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bioRxiv - Immunology 2022Quote: ... PCR fragments were assembled into a linearized pcDNA vector using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Transposed DNA was PCR amplified using the NEB Next High-Fidelity PCR Master Mix (New England Biolabs, Hitchin, UK) and primer Ad1_noMX in combination with one of six barcoded primers (Table 1 ...
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bioRxiv - Genomics 2020Quote: Quantification of several genes in sequence libraries was performed by real-time PCR using NEBnext PCR Master Mix (NEB) supplemented with SYBR Green (Applied Biosystems) ...
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bioRxiv - Genomics 2019Quote: ... 0.5 ng of genomic DNA was digested with 20 µL of MspI master mix (1 µL of MspI (NEB), 2 µL 10x NEB CutSmart Buffer in a total volume of 20 µL ...
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bioRxiv - Genomics 2020Quote: ... 0.5 μL of Ultra II Ligation Module Enhancer and 10 μL of Ultra II Ligation Module master mix (NEB) made up of a total of 20 μL with NFW ...
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bioRxiv - Developmental Biology 2019Quote: ... and the Tol2-5xUAS-TATA:M2H37A Bleeding Heart plasmid was assembled from the digested pBH and 5xUAS-TATA:M2H37A PCR product using Gibson Assembly Master Mix (NEB). The entire plasmid was sequenced to confirm ligation fidelity.
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bioRxiv - Genomics 2019Quote: ... with the amplified feature-cassette (0.2 pmol) was performed using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) in a total reaction volume of 20 µL at 50 °C for 30 min ...
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bioRxiv - Cancer Biology 2019Quote: ... The second round PCRs were performed with NEBNext® Ultra(tm) II Q5® Master Mix (NEB, cat. M0544). Amplified samples were purified with AMPure XP SPRI beads (Beckman Coulter ...
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bioRxiv - Genetics 2020Quote: ... 5.7 μl of a master mix was added such that each well got the equivalent of 3.2 μl 10X Ligase buffer (NEB), 0.25 μl T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Bioengineering 2021Quote: The LAMP reagent mix in a total volume of 25 μL contained 12.5 μL of WarmStart or Colorimetric WarmStart 2 × Master Mix (New England BioLabs) or Bsm polymerase (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... The promoter region and nanoluciferase coding region was PCR amplified from the pNL1.1 reporter constructs using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB), and 400 nM of forward and reverse primers (pNL1.1_forward 5’-AATTATCTTAAGATTTCTCTGGCCTAACTGGCCGG and pNL1.1_reverse 5’-AATTATCTTAAGTGGGTTGAAGGCTCTCAAGGGCATC) ...
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bioRxiv - Biochemistry 2021Quote: ChIP-seq library construction was performed using NEBNext ChIP-Seq Library Prep Master Mix Set (E6240, NEB, Ipswich, MA) following the manufacturer’s instruction with modifications ...
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bioRxiv - Bioengineering 2021Quote: 2 μl of genomic DNA was used to amplify strain barcodes by PCR (Q5 NEB master mix, 22 cycles) using primers containing sequence-optimized spacers to maximize nucleotide diversity in Illumina sequencing ...
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bioRxiv - Microbiology 2020Quote: ... and ligated into HindIII digested gel-purified pHERD20T (37) using isothermal assembly (Gibson Assembly Master Mix, New England BioLabs). The reaction was transformed into chemically competent DH5α and selected for on carbenicillin ...
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bioRxiv - Plant Biology 2021Quote: ... and assembled with a mammalian expression vector pcDNA3.1 Hyg(+) digested with BamHI and XhoI using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). HEK293T cells were co-transfected with constructs carrying the GA sensor GPS1 (Rizza et al. ...
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bioRxiv - Genomics 2021Quote: ... All gDNA from day 2 and 10 μg gDNA corresponding to 3 million haploid cells from day 8 was amplified in PCR reactions with maximum of 2.5 μg of DNA per reaction using NEBNext High Fidelity master mix (New England Biolabs) and target-specific primers with Illumina adaptor flanks (Supplementary Tables 1 ...
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bioRxiv - Genetics 2020Quote: ... the suspension was then mixed with 200 μl of master mix containing 50 μl 10x T4 ligase buffer (NEB), 2.5 μl 20 mg/ml BSA ...
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bioRxiv - Microbiology 2019Quote: ... The two PCR products were then assembled into the vector using the NEBuilder HiFi DNA Assembly Master Mix (NEB).
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bioRxiv - Zoology 2021Quote: ... The PCR mixtures for first step and nested PCR step (12.5 μl) contained 1X OneTaq Hot Start Master Mix (NEB) and 0.2 μM of each primer ...
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bioRxiv - Microbiology 2021Quote: ... The highly purified fragments were used for assembly using the Gibson assembly master mix (New England Biolabs, Tokyo, Japan). First ...
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bioRxiv - Genomics 2020Quote: ... a PCR reaction was run on the diluted sample using 25μL Q5 Hot Start PCR Master Mix (M0494S, NEB) and 2.5μL (20μM ...
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bioRxiv - Cancer Biology 2022Quote: ... Following amplification using Cell3™ Target: DNA Target Enrichment kit and NEBNext® Q5U® Master Mix (NEB, # M0597), DNA quality and quantity were measured using TapeStation 4200 system ...
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bioRxiv - Developmental Biology 2022Quote: ... The inserts with the mutations were assembled using the NEBuilder HiFi assembly master mix (New England Biolabs, Cat# E2621L) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and three fragments covering the full-length genome were synthesized with high-fidelity Phusion HiFi PCR Master Mix (NEB,) using specific primers as shown by Yang et al ...
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bioRxiv - Genetics 2022Quote: ... subcloning the resulting PCR product into the NcoI and EcoRI sites of pHIS.parallel (Sheffield et al., 1999) using HiFi DNA assembly master mix (NEB), and sequence verifying the resulting plasmid to confirm the absence of any PCR-induced errors.
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bioRxiv - Microbiology 2022Quote: ... The gel-purified PCR products were assembled together using NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... ChIP-seq libraries were prepared using NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (New England BioLabs). Rabbit polyclonal antibody for Rad21 has been described in39 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Filled-in chromatin was then ligated by adding 970 μl of Ligation Master Mix (150 μl of 10x NEB T4 DNA ligase buffer with ATP (NEB ...
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bioRxiv - Microbiology 2021Quote: ... cDNA across the samples was normalised and specific transcripts amplified using Phusion® high-fidelity PCR master mix (NEB) with primers outlined in Supplemental Table S2 ...
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bioRxiv - Microbiology 2021Quote: ... First-round PCR of 14 cycles was performed using Q5 High-Fidelity Master Mix (New England BioLabs, Ipswich, MA) and nested gene-specific primers at 20 μM ...
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bioRxiv - Cancer Biology 2021Quote: ... and genome-integrated sgRNA sequences were amplified by PCR using NEBNext Ultra II Q5 Master Mix (New England Biolabs). i5 and i7 multiplexing barcodes were added in a second round of PCR and final gel-purified products were sequenced on an Illumina NextSeq500 system at the LTRI NBCC facility (https://nbcc.lunenfeld.ca/ ...
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bioRxiv - Cancer Biology 2021Quote: PCR amplifications were carried out using Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB, Cat#M0531L) according to the manufacturer’s instructions (25 µl reaction) ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial 16S rRNA V4 fragments were PCR amplified using NEBNext Q5 Hot Start HiFi PCR master mix (NEB, U.K.) with universal 16S rRNA V4 primers [564F (TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-AYTGGGYDTAAAGNG ...
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bioRxiv - Cancer Biology 2022Quote: ... sequencing adapters were added to the library preparations and ligated with Blunt/TA Ligase Master Mix (New England Biolabs), followed by further quality control using a Qubit ...
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bioRxiv - Genomics 2019Quote: ... We added 1 μL 12.5% Triton-X to each well to quench the SDS and 12.5 μL NEBNext High-Fidelity 2× PCR Master Mix (NEB). Samples were PCR-amplified for 12 cycles (72 °C 5 min ...
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bioRxiv - Molecular Biology 2019Quote: ... The samples were then transferred on to the ice and the components (15 μl Blunt ligase master mix, 2.5 μl NEB Next adaptor for Illumina ...
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bioRxiv - Cell Biology 2019Quote: ... was cloned to the C-terminus of pHluo_M153R-CD63 in pLenti6/V5-DEST using Gibson Assembly Master Mix (NEB) with two pairs of primers (Forward ...
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bioRxiv - Immunology 2019Quote: ... First-round Ig-encoding sequence amplification (20 cycles) was performed using Q5 High-Fidelity Master Mix (New England BioLabs) and nested gene-specific primers ...
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bioRxiv - Molecular Biology 2020Quote: ... Targeting constructs with appropriate homology arms were generated by Gibson assembly using the Gibson Assembly Master Mix kit (NEB). Targeting constructs were designed such that Cas9 recognition sites were disrupted by the presence of the LoxP sites ...
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bioRxiv - Genomics 2020Quote: ... Locus-specific PCR was performed using the standard protocol for Q5 Hot Start Master Mix (New England BioLabs M094S). PCR was also performed on unedited DNA extracted from PGP1 iPSCs as a control for background.
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bioRxiv - Microbiology 2020Quote: ... The resulting PCR product was cloned into pDC_A1G3 pre-digested with Sal I using Gibson Assembly master mix (NEB) according to the manufacturer’s instructions giving rise to plasmid pDC_A1G2/3.
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bioRxiv - Evolutionary Biology 2020Quote: ... were adjusted to 2.5 µL and mixed with 7.5 µL of Gibson assembly master mix prepared accordingly with manufacturer instructions (NEB, USA). Reactions were incubated for 1 hour at 50°C ...
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bioRxiv - Cell Biology 2020Quote: ... EmGFP and Rab6 PCR products were gel purified and ligated using NEBuilder HiFi DNA assembly master mix as per manufacturer’s instructions (New England BioLabs). Plasmids were transfected into NEB5α bacteria and positive clones screened by PCR and verified by Sanger sequencing.
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bioRxiv - Cell Biology 2020Quote: ... Duplexed ultramers were diluted 1:100 in molecular biology grade water and ligated to digested plasmid using NEBuilder HiFi DNA assembly master mix as per manufacturer’s instructions (New England BioLabs). Plasmids were transfected into NEB5α bacteria and verified by Sanger sequencing.
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bioRxiv - Synthetic Biology 2021Quote: ... USA). DNA amplification from a single colony (i.e. colony PCR) was performed with One Taq 2× Master Mix (NEB). Electrocompetent P ...
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bioRxiv - Biochemistry 2020Quote: ... was constructed by assembling two fragments amplified from pON106 using two primer pairs (NeoRH1 and P5_A5_reverse, P5_A5_forward and NeoRH2) with Gibson Assembly Master Mix (New England Biolabs, Ipswich, MA, USA). Plasmid No ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant plasmids were constructed using the Gibson assembly method (51) following the manufacturer’s instructions for the Gibson assembly master mix (NEB). DNA fragments ymfM ...
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bioRxiv - Genomics 2019Quote: ... We added 1 µL 12.5% Triton-X to each well to quench the SDS and 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB). Samples were PCR-amplified (72 °C 5 min ...