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Citations for New England Biolabs :
2801 - 2850 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was then diluted 1:10 with nuclease free water prior to adding 1 µL to a PCR reaction containing 500 uM forward and reverse primer and 1X Q5 Master Mix (NEB). Reactions were cycled 35 times with annealing temperatures calculated by NEB Tm calculator prior to loading on 2% agarose gels ...
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bioRxiv - Molecular Biology 2020Quote: ... Lipidation-deficient LC3B proteins were generated by mutagenic PCR using Q5 Hot Start High-Fidelity Master Mix (New England Biolabs) to introduce the GGG to GCT change that leads to a glycine to alanine (G120A ...
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bioRxiv - Cell Biology 2020Quote: ... GFP and AID were amplified via PCR and assembled via Gibson cloning (using the Gibson Assembly Master Mix, cat# E2611, NEB). The resulting plasmid was sequenced thoroughly ...
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bioRxiv - Immunology 2021Quote: ... PCR-product and linearized vector containing the constant part of IgG1 heavy or kappa/lambda light chain sequences respectively were assembled using Gibson cloning with HiFi DNA Assembly Master Mix (NEB). Cloning was considered successful when sequence identity was >99.5% as verified by the cBASE module of BASE software ...
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bioRxiv - Immunology 2021Quote: ... PCR products were purified and cloned into human-IgG (Heavy, Kappa or Lambda) expression plasmids(von Boehmer et al., 2016) using the Gibson Assembly Master Mix (NEB) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: VH and VL sequences of candidate sequences were cloned into a pcDNA.3 based vector with dual CMV promotor harboring IgG1 heavy chain and light chain backbone using NEBuilder Hifi DNA Assembly Master Mix (New England Biolabs).
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bioRxiv - Genetics 2021Quote: ... input genomic DNA was amplified in a 20 μL reaction for 25 cycles using NEBNext High-Fidelity 2× PCR Master Mix (NEB). PCR products were purified using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: ... Illumina adaptors for paired-end sequencing were ligated using the NEB Next DNA Library Prep Master Mix for Illumina kit (New England Biolabs). After each step ...
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bioRxiv - Genomics 2021Quote: ... UK) and NEBNext End repair/dA-tailing Module NEB Blunt/TA Ligase Master Mix (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... This gBlocks fragment and a NdeI-HindIII-digested pET21b backbone were assembled together using a 2× Gibson master mix (NEB). Gibson assembly was possible due to a 23-bp sequence shared between the NdeI-HindIII-cut pET21b backbone and the gBlocks fragment ...
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bioRxiv - Microbiology 2020Quote: ... Two and a half μL of each fragment at equimolar concentration was added to 5 μL 2× Gibson master mix (NEB), and the mixture was incubated at 50°C for 60 min ...
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bioRxiv - Molecular Biology 2021Quote: ... These gBlocks were assembled into an expression vector on the pETDuet-1 plasmid backbone with the help of the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). First ...
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bioRxiv - Evolutionary Biology 2021Quote: ... this region was amplified using 10 separate pools of 33 nt primers containing NNN in the middle of the primer and using Gibson Assembly Master Mix (NEB) was cloned into pNL4-3 rev-in-nef after digesting by BamHI and XhoI ...
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bioRxiv - Microbiology 2021Quote: ... The resulting upstream and downstream fragments were cloned into digested (BamHI and SacI) pMSR0 vector using Gibson Assembly Master Mix (New England BioLabs). The desired clones were confirmed by PCR and sequencing of the inserts ...
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bioRxiv - Developmental Biology 2021Quote: ... and inserted in the PhiC31 integration pattB cloning vector (Potter et al., 2010) digested with XbaI-BamHI using the Gibson assembly method (Gibson Assembly Master mix NEB). To generate the pattB -sqhT20A construct ...
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bioRxiv - Genomics 2020Quote: ... 0.8 μM sequencing index primer and Q5 high-fidelity 1x master mix (New England Biolabs, Ipswich, MA, Cat. No. M0492) were added to perform PCR amplification ...
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bioRxiv - Genomics 2020Quote: ... Beads containing the ssDNA extension products were suspended in 10 μl of polyadenylation master mix containing 2 units of terminal transferase TdT (New England Biolabs), 1x TdT buffer ...
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bioRxiv - Biochemistry 2022Quote: ... trcP or fragments of the trcP ORF were cloned into pHL100’s SmaI site with the 23 nt upstream sequence and the Flag tag sequence on the C-terminal using NEBuilder HiFi DNA Assembly Master Mix (NEB); subsequently ...
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bioRxiv - Biochemistry 2022Quote: Reporter protein-expressing plasmids for measuring Tyr decoding ability were generated by integrating frameshift sequences testing Tyr codon decoding and linearized from pMMB207 encoding mCherry and bright GFP using NEBuilder HiFi DNA Assembly Master Mix (NEB).
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bioRxiv - Biophysics 2022Quote: ... Oct4 was cloned into the pOPIN expression vector using the SLIC method and Phusion Flash High-Fidelity PCR Master Mix (Finnzymes/New England Biolabs). SLIC reactions were then transformed into One Shot™ OmniMAC™ 2 T1® Chemically Competent E ...
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bioRxiv - Microbiology 2022Quote: ... and amplicons were generated by PCR (primers 341F and 806R) using a Phusion High-Fidelity PCR Master Mix (New England Biolabs). Amplification product quality was assessed by gel electrophoreses and samples were pooled in equimolar ratios ...
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bioRxiv - Neuroscience 2021Quote: ... Resulting PCR products were gel purified using Roti-Prep Gel Extraction (Carl Roth) and assembled with Bbsl-linearized pCFD6 backbone using NEBuilder HiFi DNA Assembly Master Mix (NEB) or In-Fusion HD Enzyme Premix (Takara Bio) ...
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bioRxiv - Neuroscience 2021Quote: ... was amplified from the LRP1 plasmid15 and inserted into the TOPO genomic sequence plasmid to replace the stop codon using NEBuilder HiFi DNA assembly master mix (New England Biolabs). CRIPSR guide RNA (gRNA ...
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bioRxiv - Molecular Biology 2020Quote: ... ChIP DNA was subject to library prep using NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (NEB E6240) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA was cleaved from the beads with DpnII and libraries were prepared for sequencing using the NEBNext DNA Library Prep Master Mix Set for Illumina (New England BioLabs).
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bioRxiv - Molecular Biology 2019Quote: ... a DNA fragment containing TDP-43 was inserted into pCold I-GstTev-HA by NEBuilder HiFi DNA Assembly Master Mix (NEB).
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bioRxiv - Molecular Biology 2019Quote: ... followed by the insertion of a DNA fragment containing C-terminal 2×HA tag by NEBuilder HiFi DNA Assembly Master Mix (NEB). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... and were assembled into pHaloTag-C1 to produce the pHalo-Baf in one-step using the Gibson Assembly Master Mix (New England Biolabs). The cGAS cDNA was amplified by the KOD One from pLPC-cGAS-Flag (Dou et al. ...
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bioRxiv - Genomics 2020Quote: ... centered on the candidate miR-122 ORF site was cloned with NEB HiFi DNA Assembly Master Mix (New England Biolabs) into the open reading frame of firefly luciferase in pmirGLO ...
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bioRxiv - Genomics 2020Quote: ... single-indexed libraries using the NEBNext® DNA Library Prep Master Mix Set for 454™ (E6070, New England Biolabs) and Illumina-specific adaptors52 following established protocols52–54 ...
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bioRxiv - Microbiology 2021Quote: ... Sequence-independent PCR amplification was conducted with 5 μl of purified dsDNA obtained after Klenow reaction in 50 μl of final reaction which contained 1x Q5 High-Fidelity Master Mix (NEB), 2.5 μl of 10 μM primer K (5’-GACCATCTAGCGACCTCCAC-3’ ...
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bioRxiv - Synthetic Biology 2020Quote: ... Transcriptional units were amplified directly from the golden gate mixture using 1 µL of reaction mixture as template in 50 µL Q5 Hot-Start Master Mix reactions (NEB). PCR reactions were performed according to manufacturer’s instructions and cleaned up using clean and concentrate kits (D4004-Zymo Research) ...
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bioRxiv - Molecular Biology 2019Quote: ... Targeting constructs with appropriate homology arms were generated by Gibson assembly using the Gibson Assembly Master Mix kit (New England Biolabs), or in the case of single LoxP sites with 150 bp homology arms ...
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bioRxiv - Cell Biology 2019Quote: Plasmids used in this study were cloned using the Gibson Assembly method and the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). The PSR reporter ...
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bioRxiv - Developmental Biology 2019Quote: ... and Tol2-elavl3:mCherry-CAAX was assembled from the digested plasmid and PCR products using Gibson Assembly Master Mix (NEB). The insert was sequenced to confirm ligation fidelity.
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bioRxiv - Developmental Biology 2019Quote: ... and Tol2-elavl3:GAVPO was assembled from the resulting vector and GAVPO PCR product using Gibson Assembly Master Mix (NEB). The entire plasmid was sequenced to confirm ligation fidelity.
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bioRxiv - Biochemistry 2019Quote: ... The site-directed mutagenesis reaction was performed in two parts: (i) diluted mutagenic oligonucleotides were mixed with a master mix solution composed of Hot-start Phusion DNA polymerase (NEB), GC Phusion buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Custom oligos flanking the targeted sites were used to amplify genomic DNA from pooled edited cells (Supplementary Table 7) using High-Fidelity 2× Master Mix (New England Biolabs). Indel frequencies were quantified by comparing unedited control and knockout cell lines using Inference of CRISPR Edits (ICE)73.
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bioRxiv - Developmental Biology 2019Quote: ... A short stretch of genomic region (∼600-800 bp) flanking the target site was amplified using Q5 Hot Start High Fidelity 2 × Master Mix (NEB). Amplified sequences were purified with the QIAquick PCR purification kit (Qiagen ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... We cloned our single-guide amplicon library into this vector using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs), incubating the Gibson assembly reaction at 50°C for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: The PCR reaction was motivated in 30 μL reaction systems after mixing 15μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers labelled with specific barcodes ...
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bioRxiv - Genomics 2019Quote: ... we eluted the RNA by adding 4 µl of master mix containing 1 µl of 10 mM dNTPs (New England Biolabs), 0.1 µl of 100 µM 3’ SMART reverse transcriptase (RT ...
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bioRxiv - Genomics 2020Quote: ... The oligo-pool amplicons were assembled into the linearized Lenti-gRNA-Puro plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB) for 1 h at 50 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Ligation of DNA ends between the cross-linked DNA fragments was performed by addition of 992 μL of ligation master mix (150 μL of 10x ligation buffer (B0202S, NEB), 759 μL sterile water ...
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments were ligated to the Barcode Adapter from the barcode Kit 1D (EXP-PBC001, Nanopore) using Blunt/TA Ligase Master Mix (M0367L, NEB). Following purification with AMPure XP beads ...
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bioRxiv - Cell Biology 2019Quote: ... The MADBUB plasmid was generated using Gibson cloning using the NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 100 ng of library fragment from PCR3 were used together with 100 ng linearized sgRNA expression vector for Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... of HIV env was amplified using a nested PCR approach with Phusion High-Fidelity PCR Master Mix (New England Biolabs). The outer primers were ...
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bioRxiv - Physiology 2020Quote: ... and the Esp3I fragment of the pPVxRF3 vector (a gift from S. Kondo) containing Venus and 3xP3-dsRed-Express2 using NEBuilder HiFi DNA Assembly Master Mix (NEB). The combined fragment was cloned into pBluescript ...
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bioRxiv - Microbiology 2019Quote: ... pKH12 [26] and pGR tetM/ermB [39] were amplified using Q5 DNA polymerase and assembled using NEB HiFi DNA Assembly Master Mix (New England Biolabs). For simplicity ...